Standard name
Human Ortholog
Description Mitochondrial outer membrane protein of unknown function; major constituent of the outer membrane, extending into the intermembrane space; interacts with porin (Por1p) and with Om14p; imported via the presequence pathway involving the TOM and TIM23 complexes, then assembled in the outer membrane by Mim1p; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0.07 0 0 0 0 0 0.05 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0
Cytoplasm 0.1 0.12 0.09 0.09 0 0 0 0 0 0 0 0 0.15 0.13 0.1 0.09 0.19 0.22 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.06 0 0 0 0 0 0
Endosome 0.1 0.08 0 0 0.05 0 0 0 0 0 0 0 0.12 0.11 0.16 0.18 0.11 0.15 0.09 0.09 0.08 0.08 0.09 0.09
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.13 0.17 0.1 0.08 0 0 0 0 0 0 0
Mitochondria 0.67 0.71 0.87 0.85 0.89 0.91 0.87 0.86 0.92 0.91 0.95 0.95 0.44 0.47 0.43 0.22 0.11 0.14 0.71 0.67 0.77 0.66 0.61 0.6
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0.07 0.07 0.06 0.05 0.06 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.08 0.1 0.11 0.06 0.06 0.08 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.05 0.06 0 0.05 0 0 0 0 0 0 0 0 0.1 0.08 0.17 0.18 0.12 0.11 0 0 0 0.05 0.05 0
SpindlePole 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0.05 0 0.15 0.13 0.13 0 0 0 0 0 0
Vac/Vac Membrane 0.22 0.12 0 0 0.08 0.08 0.08 0.08 0.06 0.06 0.05 0 0.23 0.27 0.28 0.16 0.21 0.21 0.05 0.05 0 0.06 0.08 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 3 0 0 1 1 0 3 2 0 0 4 4 3 2 6 3 2 7 28 10 6 10 15
Bud 2 8 39 26 19 29 32 22 42 34 28 48 4 3 1 15 8 7 3 8 1 5 8 10
Bud Neck 0 0 2 0 0 1 0 0 0 0 0 0 0 0 0 2 1 0 1 6 1 4 8 8
Bud Site 1 2 2 3 6 17 6 5 11 6 9 12 0 0 0 1 5 1 – – – – – –
Cell Periphery 11 27 19 11 9 1 13 4 5 6 10 7 16 15 16 23 6 6 0 1 1 0 1 0
Cytoplasm 41 73 51 55 36 29 26 20 10 13 12 10 61 56 53 25 38 39 5 9 1 2 3 7
Endoplasmic Reticulum 9 13 6 3 6 6 2 4 1 3 4 7 3 5 4 11 16 11 3 7 3 4 3 4
Endosome 42 50 16 22 47 3 14 18 1 12 0 5 46 49 81 52 23 27 41 65 49 29 36 42
Golgi 5 11 1 2 1 0 1 4 1 1 4 5 29 56 84 28 17 4 16 30 14 13 15 18
Mitochondria 269 431 492 503 847 943 1103 998 825 887 855 1099 174 208 219 65 23 25 322 493 477 234 256 274
Nucleus 2 5 1 2 7 18 19 12 10 6 4 7 6 2 4 5 11 7 0 1 0 1 2 2
Nuclear Periphery 2 3 2 5 39 71 90 65 47 63 37 57 0 4 5 1 3 2 4 4 8 2 4 2
Nucleolus 2 2 2 1 37 87 123 124 57 58 70 85 1 0 4 2 1 0 1 1 1 0 4 1
Peroxisomes 21 37 21 28 13 9 13 17 5 11 3 10 41 37 86 52 24 19 12 16 24 18 21 18
SpindlePole 11 13 31 21 15 13 15 7 9 10 9 17 14 20 9 43 26 24 4 10 8 3 7 7
Vac/Vac Membrane 90 75 19 24 77 81 104 96 56 62 41 35 91 120 140 45 43 37 23 37 7 22 33 34
Unique Cell Count 401 609 568 594 957 1034 1263 1163 897 970 896 1161 395 443 509 289 204 178 457 732 624 354 423 456
Labelled Cell Count 510 753 704 706 1160 1309 1561 1399 1082 1172 1086 1408 490 578 708 376 248 211 457 732 624 354 423 456


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 5.2 4.1 4.3 5.1 5.7 6.5 7.1 8.2 8.4 9.6 10.2 6.6 7.0 7.8 6.3 6.2 6.0 6.9 6.8 7.0
Std Deviation (1e-4) 1.2 1.2 1.1 1.4 1.4 1.7 2.4 2.6 4.0 4.7 6.0 6.3 1.6 2.0 3.4 1.4 1.6 1.3 4.9 5.5 5.8
Intensity Change (Log2) – – – 0.09 0.32 0.49 0.68 0.8 1.01 1.05 1.24 1.33 0.7 0.78 0.93 0.64 0.6 0.55 0.75 0.75 0.79


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 -4.8 -3.9 -4.4 -5.3 -1.8 -3.0 -3.3 -2.4 -4.3 -4.9 -5.8 -1.0 -1.5 -1.4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 2.3 0 0 1.7 0 0 0 0 0 0 0 0 0
Cell Periphery 0 -3.4 -5.6 -3.5 -5.1 -4.1 -4.1 -3.0 -4.4 0.6 0 -0.2 3.0 -0.3 0
Cytoplasm 0.2 -4.2 -5.4 -6.8 -7.2 -7.3 -7.2 -7.0 -8.6 3.1 1.9 0.8 -0.2 3.7 4.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 2.7 5.0 0
Endosome 0 2.0 -4.5 -2.7 -1.8 -4.7 -2.2 -5.1 -4.3 5.5 5.3 7.5 7.8 4.7 6.2
Golgi 0 0 0 0 0 0 0 0 0 6.3 8.5 9.9 7.3 0 0
Mitochondria 0 1.1 2.9 0.4 -0.5 3.3 3.0 6.1 5.8 -14.1 -13.6 -15.1 -18.6 -19.6 -18.3
Nucleus 0 0 2.8 2.5 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 4.3 6.0 6.1 5.3 5.1 5.8 4.4 4.9 0 0 0 0 0 0
Nucleolus 0 4.2 6.7 7.4 7.8 5.7 5.5 6.4 6.2 0 0 0 0 0 0
Peroxisomes 0 -3.0 -4.0 -3.9 -3.0 -4.4 -3.4 -4.9 -4.2 4.2 3.2 7.2 7.1 4.2 3.6
SpindlePole 0 -4.3 -4.9 -5.4 -6.5 -5.1 -5.2 -5.1 -4.7 -1.4 -0.7 -3.2 4.6 3.4 3.6
Vacuole 0.6 3.7 3.6 3.9 3.9 2.5 2.6 1.2 -0.4 9.4 10.9 11.2 6.4 8.0 7.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.3911 1.1335 1.3294 0.6168 1.4579 1.3723 4.5383 4.5686 4.3356 3.2569 4.0783 4.7638 1.498 1.544 1.3388 1.2203 0.8443 1.4456
Actin 0.0073 0.0153 0.0199 0.0071 0.0222 0.0111 0.0316 0.0076 0.0246 0.0072 0.0228 0.0097 0.0256 0.023 0.0099 0.0043 0.0275 0.0051
Bud 0.0033 0.0138 0.0102 0.009 0.0044 0.0032 0.0009 0.0027 0.007 0.0017 0.0011 0.0008 0.0016 0.0061 0.0039 0.0029 0.0005 0.0017
Bud Neck 0.0016 0.0015 0.0065 0.0098 0.0006 0.0015 0.0026 0.0006 0.0006 0.0033 0.0002 0.0045 0.0022 0.0009 0.005 0.0004 0.0049 0.0041
Bud Periphery 0.0075 0.0225 0.011 0.0331 0.0181 0.0044 0.0017 0.0085 0.0204 0.0052 0.0039 0.0023 0.0033 0.0092 0.0149 0.0126 0.0011 0.0049
Bud Site 0.0096 0.0453 0.0227 0.0065 0.0026 0.0016 0.0081 0.022 0.0146 0.0032 0.0007 0.0009 0.0032 0.039 0.0105 0.0009 0.001 0.001
Cell Periphery 0.0014 0.002 0.0017 0.0027 0.0004 0.0006 0.0012 0.0008 0.0007 0.0005 0.0002 0.0004 0.001 0.0011 0.0011 0.0005 0.0004 0.0007
Cytoplasm 0.0168 0.03 0.0194 0.01 0.0159 0.0314 0.0184 0.0077 0.0042 0.0022 0.0012 0.0064 0.0172 0.026 0.0249 0.0082 0.0055 0.0215
Cytoplasmic Foci 0.0632 0.0666 0.0688 0.0399 0.091 0.0552 0.0422 0.0334 0.0324 0.028 0.034 0.0284 0.0397 0.0462 0.0549 0.0488 0.0835 0.0462
Eisosomes 0.001 0.0006 0.0009 0.0005 0.0003 0.0004 0.0018 0.0008 0.0006 0.0004 0.0002 0.0004 0.0007 0.0005 0.0007 0.0003 0.0004 0.0004
Endoplasmic Reticulum 0.0031 0.0013 0.0015 0.0009 0.0026 0.0019 0.0039 0.0023 0.0009 0.0005 0.0003 0.0003 0.0028 0.0012 0.0025 0.0008 0.0014 0.0014
Endosome 0.1148 0.0764 0.0937 0.0846 0.1257 0.0756 0.0942 0.0459 0.0416 0.0167 0.0455 0.0259 0.1168 0.0916 0.0706 0.0368 0.0548 0.0631
Golgi 0.0489 0.0388 0.0406 0.0375 0.0321 0.0328 0.0528 0.0651 0.0347 0.035 0.058 0.0382 0.0454 0.0461 0.0377 0.0364 0.0211 0.0384
Lipid Particles 0.0325 0.0064 0.0204 0.0119 0.0554 0.025 0.0319 0.0076 0.0052 0.0037 0.0035 0.0146 0.0428 0.0186 0.0223 0.0371 0.0059 0.025
Mitochondria 0.512 0.5658 0.5288 0.6394 0.4799 0.5936 0.6279 0.7334 0.7586 0.8387 0.7416 0.8108 0.5614 0.576 0.6465 0.622 0.7181 0.6871
None 0.0693 0.0536 0.0597 0.0459 0.0739 0.0754 0.0231 0.0177 0.0045 0.0098 0.0008 0.013 0.0656 0.0267 0.0358 0.142 0.0043 0.034
Nuclear Periphery 0.0125 0.0008 0.0012 0.0009 0.0036 0.0016 0.007 0.0013 0.0004 0.0002 0.0001 0.0001 0.0017 0.0006 0.0024 0.0017 0.0018 0.0014
Nucleolus 0.0055 0.0031 0.0057 0.0015 0.0011 0.002 0.0007 0.0006 0.0007 0.0003 0.0001 0.0002 0.0036 0.0022 0.0008 0.0007 0.0004 0.0009
Nucleus 0.0067 0.0025 0.0031 0.0014 0.0025 0.0031 0.0021 0.0005 0.0008 0.0002 0.0001 0.0001 0.0024 0.001 0.001 0.0006 0.001 0.0011
Peroxisomes 0.0496 0.0352 0.0635 0.0459 0.0523 0.0579 0.0343 0.0346 0.0368 0.0395 0.0818 0.0397 0.0249 0.0611 0.0347 0.0343 0.0625 0.0386
Punctate Nuclear 0.0096 0.0012 0.0041 0.0017 0.0025 0.0068 0.0032 0.0002 0.0029 0.0005 0.0001 0.0003 0.001 0.0003 0.0007 0.0026 0.0009 0.0021
Vacuole 0.016 0.0121 0.012 0.0059 0.0085 0.0095 0.0063 0.004 0.0048 0.0014 0.0018 0.0014 0.0271 0.0164 0.0123 0.0031 0.0015 0.0144
Vacuole Periphery 0.0075 0.0053 0.0044 0.004 0.0044 0.0054 0.0041 0.0025 0.0032 0.0018 0.0018 0.0015 0.0102 0.0061 0.007 0.0031 0.0015 0.0068

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 2.3338 0.8345 4.3346 6.3739 7.8672 10.2629 2.7422 11.9591 15.9036 6.2534
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency –
Standard name
Human Ortholog
Description Mitochondrial outer membrane protein of unknown function; major constituent of the outer membrane, extending into the intermembrane space; interacts with porin (Por1p) and with Om14p; imported via the presequence pathway involving the TOM and TIM23 complexes, then assembled in the outer membrane by Mim1p; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages mitochondrion (77%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Om45

Om45


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Om45-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available