Standard name
Human Ortholog
Description Integral plasma membrane protein; required for axial budding in haploid cells; localizes to the incipient bud site and bud neck; glycosylated by Pmt4p; potential Cdc28p substrate
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.1 0.26 0.11 0.09 0 0 – 0 0 0 0 0 0.29 0.24 0.19 0.12 0.05 0.13 0.08 0.09 0.08 0.07 0 0
Bud 0 0.05 0.06 0.06 0 0 – 0.08 0.08 0.09 0.08 0.08 0.09 0.06 0.05 0 0 0 0.06 0.08 0.05 0.05 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0.11 0.09 0.15 0 0 0 0.18 0.17 0.22 0.22 0.07 0
Bud Site 0.11 0.16 0.11 0.13 0 0.12 – 0.07 0.1 0.07 0.09 0.07 0.27 0.27 0.18 0.07 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.07 0.1 0.1 0.17 0.18 0.25 – 0.27 0.31 0.28 0.41 0.36 0.18 0.09 0.14 0.18 0.25 0.27 0 0 0 0 0 0.05
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0
Endosome 0.11 0 0.11 0.08 0.15 0.11 – 0.11 0 0 0 0 0 0 0 0.05 0.06 0 0.07 0.06 0.07 0.05 0.05 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.13 0.2 0.11 0.15 0.1 0.18 – 0.18 0.32 0.34 0.31 0.32 0 0 0 0.13 0.1 0.08 0 0 0 0.05 0.06 0.07
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0.06 0.07 0 0.05 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0.05 0.08 – 0.11 0.05 0.07 0 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.22 0.16 0.35 0.29 0.18 0.18 – 0.06 0.1 0.09 0.06 0.06 0.13 0.12 0.14 0.19 0.08 0.14 0.06 0.05 0.06 0 0 0
SpindlePole 0.09 0 0.12 0.1 0 0.07 – 0.09 0 0.05 0 0.06 0.33 0.53 0.39 0.15 0.14 0.14 0 0 0 0 0 0
Vac/Vac Membrane 0.35 0.34 0.29 0.24 0.43 0.3 – 0.3 0.23 0.25 0.16 0.16 0 0 0 0.34 0.39 0.31 0.48 0.46 0.43 0.5 0.69 0.69
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 44 70 36 21 6 16 – 10 23 15 10 11 59 41 52 47 13 20 48 37 39 15 9 8
Bud 14 14 20 13 7 14 – 59 45 53 39 48 18 11 13 9 3 2 35 33 23 9 11 10
Bud Neck 0 0 1 3 0 0 – 1 1 0 1 1 23 16 42 1 1 0 113 72 106 45 26 10
Bud Site 49 44 36 29 15 55 – 51 56 45 47 46 54 47 50 29 7 4 – – – – – –
Cell Periphery 4 0 1 0 1 2 – 3 2 1 0 1 0 0 0 2 1 0 0 0 0 0 0 0
Cytoplasm 30 28 35 38 65 118 – 191 175 175 204 228 36 15 38 71 63 42 0 3 3 2 7 18
Endoplasmic Reticulum 5 5 1 2 5 9 – 6 10 6 16 4 3 1 4 19 12 6 4 4 4 2 11 13
Endosome 49 7 36 18 56 54 – 75 9 21 10 19 2 2 6 19 15 7 45 25 32 9 17 16
Golgi 5 2 2 2 1 0 – 1 2 0 0 1 1 0 0 4 7 5 9 5 2 1 2 4
Mitochondria 57 54 39 33 35 83 – 127 179 208 154 203 1 1 0 52 26 13 27 10 14 10 23 26
Nucleus 12 2 1 2 5 13 – 26 10 11 10 14 4 11 20 13 13 6 0 2 2 0 1 0
Nuclear Periphery 1 3 2 1 2 2 – 8 2 10 5 9 0 1 2 0 0 0 4 2 9 2 2 4
Nucleolus 17 7 1 7 20 36 – 77 26 42 17 58 3 4 3 3 1 2 0 0 0 0 0 0
Peroxisomes 94 45 120 64 66 86 – 42 54 54 28 41 26 20 38 76 20 22 39 20 28 1 2 0
SpindlePole 39 5 42 22 16 32 – 63 15 30 21 41 68 91 106 62 34 22 2 4 4 0 0 1
Vac/Vac Membrane 153 92 99 54 157 144 – 209 132 156 78 104 8 2 9 138 98 48 303 195 203 100 258 262
Unique Cell Count 431 274 342 222 365 473 707 565 619 495 633 203 172 274 404 249 156 638 420 479 203 377 379
Labelled Cell Count 573 378 472 309 457 664 949 741 827 640 829 306 263 383 545 314 199 638 420 479 203 377 379


Ambiguous, Bud, Bud Neck, Cell Periphery, Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.0 6.9 5.7 5.6 6.0 5.2 – 5.2 4.8 4.6 4.5 4.5 6.5 6.3 6.3 6.0 6.7 7.6 6.9 6.7 6.7
Std Deviation (1e-4) 1.5 1.3 1.4 1.3 1.7 1.5 – 1.4 1.3 1.3 1.2 1.2 1.3 1.7 1.2 1.3 1.8 2.0 1.9 1.5 1.4
Intensity Change (Log2) – – – -0.03 0.06 -0.16 – -0.14 -0.27 -0.33 -0.36 -0.36 0.19 0.12 0.14 0.07 0.23 0.41 0.26 0.23 0.22


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.4 -5.0 -4.1 – -6.8 0 0 0 0 5.5 4.0 3.0 0.5 -2.3 0.8
Bud 0 -2.7 -2.0 – 1.4 0 0 0 0 1.3 0.2 -0.6 -2.5 -2.9 -2.3
Bud Neck 0 0 0 – 0 0 0 0 0 6.1 5.4 7.3 0 0 0
Bud Site 0.9 -3.3 0.5 – -1.8 0 0 0 0 4.9 4.9 2.7 -1.6 -3.6 -3.0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.4 2.9 5.3 – 6.2 7.2 6.5 9.8 8.7 2.5 -0.5 1.4 2.9 4.9 4.8
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 3.7 3.7 0
Endosome -1.0 1.9 0.4 – 0 0 0 0 0 -4.2 -3.8 -4.1 -3.0 -1.9 -2.2
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.2 -0.8 2.4 – 2.7 0 0 0 0 -4.7 -4.3 -5.8 0.6 -0.4 -1.0
Nucleus 0 0 2.7 – 3.2 0 0 0 2.3 0 0 4.8 2.9 3.9 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 4.1 5.0 – 6.1 0 0 0 0 0 0 0 0 0 0
Peroxisomes -1.5 -5.1 -5.5 – -12.2 0 0 0 0 -5.7 -5.6 -6.0 -5.0 -7.6 -4.8
SpindlePole -0.9 -3.8 -2.7 – -1.7 0 0 0 0 6.0 9.9 7.6 1.2 0.5 0.6
Vacuole -1.2 3.9 0.5 – 0.2 -1.9 -1.3 -4.6 -4.6 -7.1 -7.5 -8.3 1.5 2.7 0.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.1895 3.8635 3.9822 3.2343 2.7589 3.4781 3.4871 4.7937 5.5495 4.8517 4.4772 4.765 3.4544 5.9831 6.1805 5.5991 5.5361 5.5214
Actin 0.0924 0.0142 0.0596 0.0019 0.0274 0.0251 0.0444 0.0044 0.0212 0.0004 0.0015 0.0317 0.04 0.0253 0.0223 0.0036 0.0076 0.0108
Bud 0.0086 0.009 0.0051 0.0018 0.0024 0.0023 0.0091 0.0068 0.004 0.0005 0.0008 0.0021 0.0111 0.0049 0.0029 0.0008 0.0011 0.0018
Bud Neck 0.1997 0.116 0.5734 0.8547 0.6906 0.7354 0.3477 0.1143 0.5913 0.9071 0.9592 0.8087 0.3059 0.1151 0.6253 0.9634 0.8448 0.7798
Bud Periphery 0.0167 0.0173 0.0087 0.0041 0.0068 0.0061 0.0227 0.0088 0.0083 0.0012 0.0022 0.0067 0.0203 0.0068 0.0058 0.002 0.0033 0.0048
Bud Site 0.1541 0.5194 0.1712 0.0107 0.0219 0.0064 0.1822 0.6638 0.1969 0.0039 0.0113 0.0055 0.3081 0.5247 0.1554 0.0063 0.0044 0.0117
Cell Periphery 0.004 0.0081 0.0067 0.0032 0.0061 0.0034 0.0067 0.007 0.0048 0.0017 0.0026 0.0028 0.0063 0.0073 0.0069 0.0016 0.005 0.0048
Cytoplasm 0.0195 0.0348 0.0029 0.0013 0.0022 0.0049 0.0199 0.0264 0.006 0.0011 0.0009 0.0058 0.0359 0.0386 0.0053 0.0007 0.0018 0.0178
Cytoplasmic Foci 0.1053 0.0375 0.0254 0.011 0.0132 0.0228 0.0815 0.0159 0.0249 0.0043 0.0027 0.0136 0.0358 0.0126 0.0144 0.0041 0.026 0.0093
Eisosomes 0.0007 0.0002 0.0006 0.0001 0.0013 0.0001 0.0013 0.0002 0.0003 0 0 0.0003 0.0005 0.0002 0.0002 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0129 0.0019 0.0011 0.0003 0.002 0.0013 0.0198 0.0021 0.001 0.0002 0.0001 0.001 0.0027 0.0055 0.0011 0.0001 0.0003 0.0018
Endosome 0.11 0.0442 0.0251 0.0267 0.0548 0.0419 0.0609 0.0232 0.024 0.0154 0.0033 0.0194 0.0409 0.0329 0.0343 0.0039 0.0276 0.0249
Golgi 0.0411 0.0131 0.0175 0.0137 0.0275 0.0255 0.016 0.0039 0.0122 0.0031 0.0023 0.0128 0.0073 0.0045 0.0129 0.0041 0.0131 0.0067
Lipid Particles 0.0482 0.0081 0.0173 0.0109 0.0107 0.0105 0.0204 0.0024 0.0085 0.0016 0.0005 0.0015 0.005 0.0039 0.005 0.0009 0.0078 0.0018
Mitochondria 0.0135 0.0106 0.0094 0.0125 0.0492 0.0057 0.0117 0.0045 0.0038 0.0012 0.0005 0.0056 0.0041 0.0026 0.0043 0.0003 0.0044 0.0027
None 0.0065 0.0033 0.0007 0.0002 0.0009 0.0004 0.0441 0.0389 0.017 0 0.0001 0.024 0.0426 0.0228 0.002 0 0.0005 0.0031
Nuclear Periphery 0.0527 0.0014 0.0007 0.0002 0.0026 0.0012 0.0277 0.0014 0.0018 0.0001 0 0.0004 0.0013 0.0048 0.0005 0 0.0001 0.0006
Nucleolus 0.0063 0.0074 0.0055 0.0089 0.0038 0.0018 0.0053 0.0032 0.0039 0.003 0.0036 0.0016 0.0035 0.0054 0.0058 0.0017 0.0036 0.0026
Nucleus 0.0116 0.0047 0.0023 0.0021 0.0027 0.0016 0.0078 0.0034 0.0053 0.0013 0.0009 0.0012 0.0035 0.0114 0.0024 0.0006 0.0016 0.0018
Peroxisomes 0.0348 0.0071 0.0262 0.0052 0.0036 0.0071 0.0172 0.0031 0.0079 0.0007 0.0007 0.0031 0.0078 0.0023 0.0031 0.0012 0.0099 0.0021
Punctate Nuclear 0.0094 0.0012 0.0013 0.0005 0.0011 0.001 0.006 0.0012 0.0038 0.0002 0.0002 0.0004 0.0017 0.0035 0.0007 0.0003 0.0008 0.0003
Vacuole 0.045 0.1342 0.0357 0.0263 0.0561 0.0872 0.0421 0.0615 0.049 0.0478 0.006 0.0463 0.1071 0.1605 0.0831 0.0038 0.0338 0.1019
Vacuole Periphery 0.007 0.0063 0.0036 0.0036 0.0131 0.0083 0.0053 0.0033 0.0041 0.005 0.0004 0.0056 0.0086 0.0044 0.0061 0.0003 0.0023 0.0087

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 39.9493 8.7784 2.9609 9.2635 37.3279 32.1202 12.9103 8.0329 7.2491 41.9323
Translational Efficiency 1.6513 1.3903 1.1089 0.789 1.3831 2.3131 1.1154 1.0435 1.051 1.3674

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✔
Standard name
Human Ortholog
Description Integral plasma membrane protein; required for axial budding in haploid cells; localizes to the incipient bud site and bud neck; glycosylated by Pmt4p; potential Cdc28p substrate
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Axl2

Axl2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Axl2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available