Standard name
Human Ortholog
Description Pantothenate synthase; also known as pantoate-beta-alanine ligase, required for pantothenic acid biosynthesis, deletion causes pantothenic acid auxotrophy, homologous to E. coli panC
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.95 – 1.0 0.97 0.97 0.91 0.81 0.75 0.69 0.75 0.73 0.66 0.98 0.97 1.0 0.94 0.94 0.96 0.94 0.9 0.88 0.94 0.93 0.93
Endoplasmic Reticulum 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 – 0 0 0 0 0.05 0 0.14 0.12 0.22 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 – 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0.05 0 0 0
Nuclear Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 – 0 0 0 0.05 0.12 0.17 0.19 0.12 0.12 0.2 0 0 0 0.08 0.05 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 0 0 2 0 0 1 1 2 0 0 0 0 1 0 0 0 0 0 0 1
Bud 0 – 0 0 0 1 1 3 1 1 4 1 0 0 0 1 0 0 0 0 0 0 0 1
Bud Neck 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 – 0 0 3 7 6 8 3 6 6 6 2 2 3 2 7 11 0 0 0 0 1 0
Cytoplasm 207 – 20 195 242 204 316 253 151 161 157 199 203 204 214 301 171 311 206 29 69 86 230 325
Endoplasmic Reticulum 1 – 0 1 1 4 13 11 5 6 0 12 1 0 0 4 4 9 0 0 0 0 3 5
Endosome 0 – 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1
Golgi 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1 0 0 0 0 0
Mitochondria 2 – 0 0 0 0 21 14 31 25 48 26 0 0 0 0 0 1 0 0 0 0 0 1
Nucleus 4 – 0 2 1 9 7 8 5 9 6 15 3 0 1 4 3 1 3 0 3 1 0 1
Nuclear Periphery 1 – 0 0 0 0 0 0 0 2 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 – 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 8 – 0 3 9 12 45 56 42 25 27 59 0 9 1 24 9 12 2 1 2 0 3 3
Unique Cell Count 218 20 201 250 224 389 336 219 216 216 300 207 210 214 319 182 324 221 33 79 92 248 349
Labelled Cell Count 226 20 201 256 237 412 353 238 236 249 323 209 215 219 337 195 346 221 33 79 92 248 349


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 13.1 – 11.7 13.5 12.2 10.8 9.7 10.4 9.0 9.0 7.9 9.0 16.5 15.7 15.7 20.5 22.4 22.5 12.8 14.0 14.5
Std Deviation (1e-4) 2.3 – 1.7 2.3 1.9 1.7 1.7 2.1 2.0 1.6 1.5 1.8 2.6 2.8 2.5 4.5 4.6 4.8 2.7 3.4 3.1
Intensity Change (Log2) – – – 0.2 0.06 -0.12 -0.27 -0.17 -0.39 -0.39 -0.56 -0.38 0.49 0.42 0.42 0.81 0.94 0.94 0.13 0.26 0.31


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.8 -0.8 -1.4 -2.1 -2.5 -2.9 -2.6 -2.7 -3.1 -0.6 -0.8 0 -1.1 -1.1 -0.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 11.6277 14.6841 12.7156 12.6944 10.9683 12.9288 10.0857 13.2876 11.7158 10.7818 8.4008 11.6715 9.716 13.1389 12.5628 9.931 10.7581 10.7476
Actin 0.0145 0.0002 0.0061 0.0013 0.0322 0.0017 0.0163 0.0001 0.0013 0.01 0.0424 0.0007 0.0128 0.0002 0.0021 0.038 0.0235 0.0106
Bud 0.0024 0.0004 0.0021 0.0025 0.0262 0.0002 0.0004 0.0001 0.0001 0.0004 0.0021 0.0002 0.0021 0.0001 0.0001 0.0014 0.0002 0.001
Bud Neck 0.0021 0.0004 0.002 0.0005 0.001 0.004 0.0009 0.0002 0.0003 0.0044 0.0021 0.0009 0.0009 0.0001 0.0002 0.0028 0.0005 0.0028
Bud Periphery 0.0009 0.0003 0.001 0.0007 0.0094 0.0003 0.0003 0 0 0.0003 0.0014 0.0001 0.002 0 0.0001 0.0026 0.0001 0.0013
Bud Site 0.0025 0.0005 0.0038 0.0011 0.0035 0.0001 0.0007 0.0001 0.0002 0.0012 0.0024 0.0001 0.0039 0 0.0003 0.0031 0.0014 0.0012
Cell Periphery 0.0002 0 0.0001 0.0001 0.0004 0 0.0001 0 0 0.0001 0.0002 0 0.0002 0 0 0.0004 0.0001 0.0002
Cytoplasm 0.8654 0.9637 0.8941 0.9262 0.7118 0.9248 0.8785 0.9771 0.9384 0.8384 0.7626 0.9438 0.8408 0.9889 0.9675 0.7435 0.9115 0.902
Cytoplasmic Foci 0.0133 0.0019 0.0143 0.0085 0.0099 0.0034 0.0176 0.0006 0.013 0.0254 0.0283 0.0035 0.0227 0.0006 0.0016 0.0436 0.0092 0.0079
Eisosomes 0.0001 0 0 0 0.0002 0 0.0002 0 0 0.0001 0.0012 0 0.0001 0 0 0.0003 0.0001 0.0001
Endoplasmic Reticulum 0.0073 0.0012 0.0053 0.0018 0.0142 0.0032 0.0038 0.0005 0.0019 0.0028 0.0036 0.0015 0.0037 0.0004 0.0046 0.0035 0.0023 0.0034
Endosome 0.0175 0.0012 0.0212 0.0167 0.0597 0.0062 0.0172 0.0002 0.0044 0.0252 0.0218 0.0034 0.0216 0.0002 0.0049 0.021 0.0046 0.0115
Golgi 0.0053 0.0001 0.0053 0.0036 0.0362 0.0014 0.0041 0 0.0009 0.0053 0.0143 0.002 0.005 0 0.0007 0.0171 0.0044 0.0044
Lipid Particles 0.0037 0 0.0025 0.0003 0.0163 0.0009 0.0054 0 0.0016 0.005 0.0347 0.0026 0.0088 0 0.0003 0.0148 0.0043 0.006
Mitochondria 0.0027 0.0002 0.005 0.0018 0.0306 0.0016 0.0023 0.0001 0.0003 0.0045 0.0203 0.0012 0.0095 0.0001 0.0024 0.0374 0.0021 0.012
None 0.0078 0.001 0.0032 0.0016 0.0022 0.0016 0.0058 0.0005 0.0018 0.0096 0.0036 0.0008 0.0046 0.0005 0.0011 0.0006 0.013 0.0017
Nuclear Periphery 0.0095 0.0023 0.0054 0.003 0.0079 0.0044 0.0052 0.0011 0.0028 0.0077 0.0064 0.0033 0.0088 0.0012 0.0035 0.0028 0.0017 0.0081
Nucleolus 0.0003 0.0001 0.0002 0.0001 0.0016 0.0001 0.0002 0 0.0001 0.0004 0.0004 0.0001 0.001 0 0 0.0003 0.0001 0.0011
Nucleus 0.036 0.0245 0.0201 0.0272 0.0192 0.0416 0.0313 0.0191 0.0255 0.0305 0.0275 0.0337 0.0282 0.0075 0.0093 0.0073 0.0047 0.0149
Peroxisomes 0.0024 0 0.003 0.0007 0.0057 0.0001 0.0042 0 0.0027 0.0235 0.0192 0.0006 0.0074 0 0.0001 0.0549 0.013 0.0037
Punctate Nuclear 0.0031 0.0007 0.0026 0.0006 0.0016 0.0016 0.0027 0.0003 0.0042 0.0019 0.0027 0.0009 0.0066 0.0001 0.0004 0.0008 0.0007 0.0033
Vacuole 0.002 0.0011 0.0022 0.0015 0.0061 0.002 0.0021 0.0001 0.0003 0.0023 0.0016 0.0005 0.0068 0.0001 0.0005 0.0021 0.002 0.0018
Vacuole Periphery 0.0009 0.0001 0.0006 0.0004 0.0038 0.0006 0.0007 0 0.0001 0.001 0.0012 0.0002 0.0023 0 0.0004 0.0016 0.0005 0.0008

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 47.4583 42.4248 48.0086 42.7969 39.2265 33.2024 52.5472 38.7229 55.5386 46.1731
Translational Efficiency 0.9153 1.1212 1.0081 1.1772 1.0806 1.4132 0.912 1.2357 0.8743 1.0327

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Pantothenate synthase; also known as pantoate-beta-alanine ligase, required for pantothenic acid biosynthesis, deletion causes pantothenic acid auxotrophy, homologous to E. coli panC
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Pan6

Pan6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pan6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available