Standard name
Human Ortholog
Description Peptidyl-prolyl cis/trans-isomerase; activator of the phosphotyrosyl phosphatase activity of PP2A; involved in G1 phase progression, microtubule dynamics, bud morphogenesis and DNA repair; required for rapid reduction of Sgs1p levels in response to rapamycin; subunit of the Tap42p-Sit4p-Rrd1p complex; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.98 0.95 0.93 0.85 0.78 0.68 0.64 0.6 0.56 0.54 0.47 0.98 0.97 0.98 0.92 0.92 0.91 0.93 0.93 0.94 0.95 0.95 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.07 0.27 0.3 0.28 0.32 0.39 0.43 0.43 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.07 0.07 0.12 0.08 0.16 0.16 0.19 0.18 0.16 0.28 0 0.07 0 0.1 0.11 0.17 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 1 0 1 6 15 7 7 16 4 14 0 0 0 1 0 0 0 1 2 0 0 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2
Bud Site 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 2 1 4 4 3 5 3 4 10 0 3 3 4 5 3 0 0 0 0 0 0
Cytoplasm 337 376 389 327 387 441 471 455 337 368 285 317 415 444 458 435 497 361 329 371 390 336 249 317
Endoplasmic Reticulum 0 2 3 0 1 1 4 4 1 5 5 1 1 2 2 13 14 5 1 0 1 0 1 1
Endosome 0 0 0 0 1 1 1 1 2 1 0 0 1 2 2 2 1 1 4 2 0 1 0 5
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 3 0 3 0 0 1
Mitochondria 8 6 3 14 33 152 205 202 180 253 225 292 0 0 0 1 9 2 5 2 1 0 1 2
Nucleus 4 7 2 3 5 11 18 19 23 29 19 29 5 3 6 25 17 7 3 8 2 6 3 5
Nuclear Periphery 0 0 0 1 0 1 6 4 8 1 0 4 0 0 0 0 0 0 0 0 0 0 0 2
Nucleolus 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
SpindlePole 1 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1
Vac/Vac Membrane 6 1 28 25 54 45 111 117 105 120 84 190 12 32 14 49 61 66 2 8 6 4 5 7
Unique Cell Count 347 384 411 352 453 566 691 714 558 652 525 679 423 460 467 473 540 397 354 400 415 353 264 356
Labelled Cell Count 357 393 426 372 485 662 835 813 668 798 626 857 434 487 485 531 604 445 354 400 415 353 264 356


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.0 6.7 6.6 6.2 6.3 5.7 5.7 5.6 5.7 5.4 5.2 5.3 8.5 8.8 8.2 10.2 8.9 8.7 8.7 9.1 9.6
Std Deviation (1e-4) 0.9 0.9 1.0 0.9 1.4 1.1 1.2 1.1 1.1 1.0 1.0 1.1 1.2 1.1 1.0 1.7 1.4 1.5 1.2 1.4 1.6
Intensity Change (Log2) – – – -0.08 -0.06 -0.22 -0.22 -0.23 -0.2 -0.28 -0.35 -0.32 0.38 0.42 0.32 0.63 0.43 0.4 0.4 0.46 0.55


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 2.6 0 0 2.8 0 2.5 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.0 -4.5 -7.2 -10.3 -11.5 -12.2 -13.4 -13.6 -16.1 2.7 1.4 2.7 -1.6 -1.6 -2.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 2.2 2.1 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.0 4.8 11.0 11.9 11.5 12.4 14.1 14.9 15.2 0 0 0 0 1.3 0
Nucleus 0 0 2.0 2.5 2.6 3.5 3.7 3.2 3.6 0 0 0 4.1 2.9 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.2 2.6 0.7 4.5 4.6 5.4 5.3 4.3 8.5 -2.7 0.1 -2.6 1.9 2.4 4.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.9933 5.2293 5.1455 4.9406 4.6478 5.1555 3.7832 4.4785 4.1005 3.3889 4.2729 4.1087 3.482 3.5329 3.2895 3.3096 2.7492 3.2945
Actin 0.0012 0.0007 0.0006 0.0005 0.0004 0.0047 0.0461 0.005 0.0262 0.0201 0.0043 0.0121 0.0431 0.001 0.0054 0.0399 0.0405 0.0075
Bud 0.0044 0.0045 0.0008 0.0004 0.0002 0.0008 0.0015 0.0006 0.0014 0.0014 0.0012 0.0014 0.0048 0.0047 0.0007 0.0052 0.0005 0.0007
Bud Neck 0.0031 0.0012 0.0008 0.001 0.0013 0.0016 0.0075 0.0005 0.0012 0.0063 0.0034 0.0033 0.001 0.0004 0.0009 0.0046 0.0011 0.0059
Bud Periphery 0.0012 0.0014 0.0002 0.0002 0.0001 0.0003 0.002 0.0002 0.0009 0.0016 0.002 0.0013 0.0107 0.0014 0.0007 0.004 0.0004 0.0011
Bud Site 0.0014 0.0082 0.0042 0.0005 0.0002 0.0003 0.0202 0.0105 0.0137 0.0066 0.0003 0.0059 0.0034 0.0095 0.0068 0.0056 0.0028 0.0024
Cell Periphery 0.0001 0.0001 0.0001 0.0001 0 0 0.0013 0.0002 0.0002 0.0009 0.0002 0.0002 0.0003 0.0001 0.0002 0.0007 0.0001 0.0001
Cytoplasm 0.793 0.8162 0.7954 0.7062 0.7917 0.7595 0.6798 0.9133 0.8252 0.819 0.7248 0.8077 0.7135 0.9149 0.8519 0.8111 0.6851 0.8343
Cytoplasmic Foci 0.011 0.0109 0.0106 0.0061 0.0104 0.011 0.0385 0.0049 0.0154 0.0245 0.0048 0.0259 0.0274 0.0162 0.0149 0.0224 0.032 0.0159
Eisosomes 0 0 0 0.0001 0 0 0.0005 0 0.0001 0.0001 0.0001 0.0001 0.0002 0 0 0.0001 0.0003 0
Endoplasmic Reticulum 0.0041 0.0035 0.0033 0.0021 0.0035 0.0024 0.0176 0.0034 0.0077 0.0098 0.0047 0.0035 0.0133 0.0029 0.0047 0.003 0.0027 0.0051
Endosome 0.0073 0.0056 0.004 0.0073 0.0059 0.0054 0.0274 0.0026 0.0311 0.0238 0.0398 0.0194 0.0284 0.0023 0.0142 0.0104 0.0154 0.0106
Golgi 0.0004 0.0003 0.0002 0.0005 0.0001 0.0009 0.0115 0.0006 0.022 0.0057 0.0114 0.0093 0.0134 0.0002 0.0019 0.0029 0.0156 0.0018
Lipid Particles 0.0002 0.0001 0.0001 0.001 0.0001 0.0001 0.0189 0.0005 0.0027 0.0031 0.0028 0.0099 0.0078 0.0008 0.001 0.0044 0.0375 0.0014
Mitochondria 0.0048 0.0007 0.0007 0.0033 0.0006 0.0009 0.0039 0.0004 0.0045 0.0037 0.0526 0.0165 0.011 0.0004 0.0068 0.0031 0.0194 0.0018
None 0.0861 0.0462 0.0952 0.1643 0.1325 0.0829 0.0386 0.0282 0.0083 0.0062 0.0042 0.0104 0.0378 0.0141 0.0346 0.0095 0.0078 0.0083
Nuclear Periphery 0.0061 0.0057 0.0057 0.0049 0.0049 0.0048 0.012 0.003 0.0045 0.0114 0.0314 0.0074 0.0199 0.0035 0.0048 0.0057 0.0043 0.0101
Nucleolus 0.0012 0.0006 0.0004 0.0061 0.0002 0.0002 0.0016 0.0001 0.0007 0.0003 0.0007 0.0009 0.0003 0.0007 0.0005 0.0047 0.0009 0.0005
Nucleus 0.0645 0.0817 0.0659 0.0881 0.0369 0.1088 0.0462 0.0213 0.024 0.0402 0.0802 0.0502 0.0389 0.0194 0.0333 0.0296 0.0448 0.0782
Peroxisomes 0.0005 0.0005 0.0005 0.0004 0.0003 0.0007 0.0119 0.0011 0.0026 0.0035 0.0002 0.0035 0.009 0.0008 0.0034 0.0016 0.0689 0.0013
Punctate Nuclear 0.007 0.0094 0.0099 0.0038 0.0083 0.0133 0.0073 0.0022 0.0037 0.0082 0.0017 0.0083 0.0125 0.0053 0.0057 0.0224 0.0183 0.0101
Vacuole 0.0019 0.0021 0.0011 0.0025 0.002 0.0012 0.0046 0.0012 0.0031 0.0026 0.0096 0.0021 0.0023 0.0012 0.0063 0.0082 0.0012 0.0025
Vacuole Periphery 0.0004 0.0003 0.0002 0.0009 0.0004 0.0002 0.0011 0.0002 0.0007 0.0008 0.0196 0.0008 0.0012 0.0002 0.0013 0.001 0.0004 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 34.5397 35.0489 26.0078 25.9989 28.4428 41.0515 35.227 43.5653 40.1307 28.289
Translational Efficiency 0.4574 0.352 0.5184 0.6229 0.4074 0.3361 0.374 0.4158 0.4713 0.5155

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Peptidyl-prolyl cis/trans-isomerase; activator of the phosphotyrosyl phosphatase activity of PP2A; involved in G1 phase progression, microtubule dynamics, bud morphogenesis and DNA repair; required for rapid reduction of Sgs1p levels in response to rapamycin; subunit of the Tap42p-Sit4p-Rrd1p complex; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress
Localization
Cell Percentages cytoplasm (96%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Rrd1

Rrd1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rrd1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available