Standard name
Human Ortholog
Description Formin; nucleates the formation of linear actin filaments; involved in processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables; activity is regulated by Hof1p and by the Bud14p-Kel1p-Kel2p complex; dephosphorylated and delocalized from the division site in a Glc7p/Ref2p-dependent manner; functionally redundant with BNI1
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.05 0 0 0 0.06 0 0 0 0 0 0 0 0.08 0.1 0.07 0 0 0 0 0 0 0 0.05 0.05
Bud 0.06 0.05 0.12 0.15 0.17 0.15 0.12 0.1 0.1 0.11 0.1 0.1 0.05 0.06 0.11 0 0 0 0.18 0.12 0.09 0 0 0
Bud Neck 0.14 0.09 0.18 0.18 0.22 0.15 0.11 0.11 0.09 0.09 0.1 0.07 0.1 0.13 0.05 0.15 0.14 0.12 0.31 0.3 0.3 0 0 0
Bud Site 0.09 0.09 0.1 0.06 0.07 0.07 0.07 0 0.06 0 0 0 0.14 0.07 0.09 0.06 0 0.07 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Cytoplasm 0.6 0.64 0.62 0.65 0.54 0.62 0.6 0.72 0.62 0.6 0.53 0.54 0.51 0.51 0.55 0.56 0.57 0.62 0.29 0.31 0.29 0.78 0.69 0.67
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.06 0.07 0.12 0.11 0.19 0.26 0.27 0.4 0.43 0.52 0.5 0 0 0 0 0 0.05 0.05 0 0.09 0.1 0.11 0.1
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.05 0 0 0 0 0 0 0 0 0 0 0 0.15 0.17 0.17 0 0.06 0.07 0 0 0 0 0 0
SpindlePole 0.18 0.12 0.11 0 0.12 0.1 0.12 0.07 0.08 0.07 0.07 0.06 0.17 0.18 0.13 0.24 0.28 0.18 0.08 0.09 0.09 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 25 10 8 3 13 7 2 4 3 0 0 0 33 43 29 8 3 8 11 14 10 7 23 26
Bud 28 23 36 15 36 47 42 29 30 37 21 31 19 24 47 14 6 10 95 68 27 4 11 15
Bud Neck 69 43 53 18 48 47 39 35 25 29 21 22 41 54 23 67 37 40 165 165 87 7 8 14
Bud Site 45 42 30 6 14 23 24 9 17 6 2 10 55 31 39 26 9 25 – – – – – –
Cell Periphery 4 0 2 0 1 2 2 2 1 4 0 2 2 3 3 10 13 10 0 1 2 3 6 7
Cytoplasm 297 308 180 64 115 196 208 219 181 199 115 175 203 214 244 242 155 213 154 171 84 255 306 341
Endoplasmic Reticulum 1 2 1 0 0 0 0 0 0 0 1 0 1 1 2 27 6 15 3 1 2 0 7 6
Endosome 4 5 1 0 0 0 0 0 0 0 0 0 12 15 18 8 1 9 13 21 10 2 4 11
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 2 0 6 2 0 2 2 3
Mitochondria 6 30 19 12 23 59 90 82 117 143 112 162 3 1 9 16 3 17 24 24 26 31 49 49
Nucleus 6 10 4 0 0 2 3 5 1 4 0 2 2 2 3 12 13 11 0 3 0 0 0 0
Nuclear Periphery 1 0 0 0 0 0 0 0 0 1 1 0 0 1 0 2 0 0 3 1 1 1 1 0
Nucleolus 3 0 0 1 0 3 1 0 1 2 0 1 1 3 0 0 2 2 2 5 0 0 0 0
Peroxisomes 23 20 4 4 1 5 4 1 4 1 0 3 61 72 74 13 16 24 2 3 2 0 1 0
SpindlePole 89 58 32 3 25 33 41 22 23 23 15 19 70 75 56 105 75 63 42 50 28 0 3 6
Vac/Vac Membrane 5 8 1 0 0 1 0 0 0 0 0 3 2 0 2 11 3 5 5 18 6 4 8 17
Unique Cell Count 495 481 289 98 214 318 349 305 294 332 217 323 400 418 441 434 270 342 537 560 296 327 443 510
Labelled Cell Count 607 559 371 126 276 425 456 408 403 449 288 430 505 539 549 565 344 452 537 560 296 327 443 510


Bud Neck

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.4 3.9 3.5 3.3 3.3 3.0 2.8 2.9 2.5 2.4 2.6 2.4 4.5 4.7 4.1 6.4 6.9 6.4 4.7 4.9 5.1
Std Deviation (1e-4) 0.7 0.6 0.8 1.4 0.9 1.1 1.2 0.8 1.1 0.7 0.9 1.1 1.1 1.2 1.2 1.7 2.2 2.0 1.5 1.9 1.5
Intensity Change (Log2) – – – -0.07 -0.07 -0.18 -0.28 -0.28 -0.46 -0.51 -0.42 -0.53 0.37 0.43 0.25 0.88 1.0 0.89 0.44 0.49 0.57


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.0908 0.8751 0.8006 0.5811 0.7614 0.8152 4.5147 3.5256 3.7985 3.2736 4.9722 3.7711 3.5333 3.1867 3.0982 3.4438 4.1366 3.493
Actin 0.0075 0.006 0.0026 0.0001 0.0012 0.0041 0.0559 0.0074 0.0283 0.0207 0.0653 0.0122 0.064 0.0201 0.0033 0.0014 0.0002 0.0123
Bud 0.0047 0.0034 0.0006 0.0001 0.0012 0.0005 0.0088 0.0059 0.0021 0.0045 0.0041 0.0016 0.0019 0.01 0.0011 0.0001 0.0002 0.0047
Bud Neck 0.0382 0.0605 0.6772 0.9245 0.8499 0.3554 0.0206 0.0412 0.6027 0.8647 0.5361 0.2874 0.0764 0.0446 0.6717 0.9962 0.5822 0.2881
Bud Periphery 0.0066 0.004 0.0005 0.0001 0.0019 0.0014 0.0147 0.0076 0.0035 0.0123 0.0082 0.0024 0.0027 0.0043 0.0015 0.0001 0.0002 0.0052
Bud Site 0.1575 0.3463 0.1833 0.0027 0.0125 0.003 0.1067 0.2965 0.2234 0.0644 0.0091 0.0116 0.2104 0.3214 0.2269 0.0006 0.0059 0.0031
Cell Periphery 0.0013 0.0033 0.0005 0.0001 0.0013 0.0007 0.0022 0.0035 0.0019 0.0013 0.0005 0.0011 0.0016 0.003 0.0013 0.0001 0.0003 0.0009
Cytoplasm 0.0951 0.1528 0.0063 0.042 0.0107 0.2401 0.0517 0.0781 0.0132 0.0016 0.0295 0.1243 0.025 0.0536 0.0093 0.0001 0.0025 0.0789
Cytoplasmic Foci 0.135 0.0309 0.028 0.0044 0.0465 0.066 0.0847 0.0205 0.0165 0.0109 0.0497 0.0302 0.0648 0.0184 0.0059 0.0005 0.1698 0.0695
Eisosomes 0.0011 0.0004 0.0006 0 0.0001 0.0003 0.001 0.0008 0.0004 0.0002 0.0007 0.0003 0.0072 0.001 0.0003 0 0.0001 0.0007
Endoplasmic Reticulum 0.0012 0.0023 0.0002 0.0001 0.0002 0.0014 0.0097 0.0012 0.0017 0.0005 0.0052 0.0013 0.0013 0.0017 0.0001 0 0.0001 0.0013
Endosome 0.0023 0.0049 0.0009 0.0002 0.0018 0.005 0.029 0.0015 0.0066 0.0005 0.0313 0.0039 0.0025 0.0012 0.0001 0 0.0076 0.0039
Golgi 0.0011 0.0008 0.0004 0.0001 0.0008 0.0007 0.0048 0.0004 0.0056 0.0004 0.0092 0.0017 0.0029 0.0006 0.0002 0 0.0017 0.0023
Lipid Particles 0.0041 0.0015 0.0008 0.0001 0.0015 0.0022 0.0131 0.0011 0.0045 0.0003 0.0139 0.0043 0.0065 0.001 0.0002 0 0.0034 0.0032
Mitochondria 0.0007 0.0007 0.0002 0 0.001 0.0003 0.0052 0.0009 0.0025 0.0002 0.0054 0.0018 0.0065 0.0043 0.0002 0 0.0001 0.0094
None 0.5103 0.3385 0.0787 0.0219 0.0233 0.3081 0.4554 0.5073 0.0532 0.001 0.0982 0.492 0.4862 0.4887 0.0736 0.0001 0.1479 0.4992
Nuclear Periphery 0.0002 0.0005 0.0004 0 0.0002 0.0002 0.0379 0.0005 0.0012 0.0003 0.0193 0.0024 0.0009 0.0007 0 0 0 0.0004
Nucleolus 0.0029 0.0076 0.0025 0.001 0.0128 0.0009 0.008 0.0043 0.0051 0.0046 0.0072 0.0035 0.0139 0.004 0.0016 0.0003 0.0327 0.0011
Nucleus 0.0013 0.0136 0.0011 0.0006 0.0104 0.0011 0.0263 0.0043 0.0029 0.0041 0.0283 0.003 0.0051 0.0108 0.0008 0.0002 0.0102 0.0013
Peroxisomes 0.0101 0.0024 0.0013 0 0.0012 0.0014 0.0313 0.0018 0.0089 0.0002 0.0166 0.0041 0.008 0.0013 0.0004 0 0.0013 0.0045
Punctate Nuclear 0.0173 0.0138 0.0136 0.0018 0.0179 0.0056 0.0241 0.0109 0.0144 0.0062 0.0547 0.0074 0.01 0.0076 0.001 0.0003 0.032 0.0085
Vacuole 0.0014 0.0055 0.0003 0.0001 0.0032 0.0015 0.0062 0.004 0.001 0.0008 0.0056 0.0031 0.0018 0.0016 0.0004 0 0.0017 0.0011
Vacuole Periphery 0.0001 0.0002 0 0 0.0003 0.0001 0.0027 0.0003 0.0002 0.0001 0.0018 0.0003 0.0005 0.0002 0 0 0 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.0928 5.4958 6.7378 9.9419 10.5702 8.2816 6.946 6.3595 8.5969 13.0029
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency –
Standard name
Human Ortholog
Description Formin; nucleates the formation of linear actin filaments; involved in processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables; activity is regulated by Hof1p and by the Bud14p-Kel1p-Kel2p complex; dephosphorylated and delocalized from the division site in a Glc7p/Ref2p-dependent manner; functionally redundant with BNI1
Localization
Cell Percentages cytoplasm (24%), bud neck (20%), mixed (7%)
Cell Cycle Regulation Yes
cytoplasm - T/G1 (5.2e-11)
bud neck - S/G2 (5.1e-05)
bud neck - MA (1.7e-06)
Subcompartmental Group N/A

Bnr1

Bnr1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bnr1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available