Standard name | |
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Human Ortholog | |
Description | Putative membrane glycoprotein; has strong similarity to Vth2p and Pep1p/Vps10p; may be involved in vacuolar protein sorting |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 4 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 5 | 5 | 2 | – | – | – | 14 | 25 | 4 | 6 | 8 | 9 |
Bud | 4 | 13 | 4 | 1 | 3 | 4 | – | 8 | 6 | 8 | 9 | 7 | 3 | 1 | 0 | – | – | – | 8 | 11 | 0 | 5 | 15 | 10 |
Bud Neck | 1 | 0 | 4 | 0 | 0 | 1 | – | 4 | 0 | 1 | 1 | 1 | 4 | 5 | 0 | – | – | – | 2 | 8 | 2 | 1 | 4 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | – | – | – | – | – | – | – | – | – |
Cell Periphery | 1 | 1 | 0 | 0 | 0 | 2 | – | 0 | 0 | 1 | 1 | 2 | 4 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 1 | 0 |
Cytoplasm | 306 | 486 | 217 | 125 | 177 | 243 | – | 278 | 257 | 298 | 148 | 160 | 227 | 296 | 293 | – | – | – | 93 | 146 | 68 | 71 | 118 | 91 |
Endoplasmic Reticulum | 5 | 27 | 1 | 1 | 0 | 0 | – | 2 | 0 | 0 | 0 | 0 | 48 | 19 | 18 | – | – | – | 7 | 8 | 5 | 8 | 13 | 16 |
Endosome | 162 | 176 | 59 | 18 | 17 | 1 | – | 2 | 0 | 0 | 0 | 0 | 13 | 21 | 30 | – | – | – | 306 | 455 | 135 | 160 | 283 | 202 |
Golgi | 4 | 3 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 9 | 7 | 2 | 4 | 8 | 2 |
Mitochondria | 19 | 49 | 8 | 12 | 9 | 66 | – | 98 | 128 | 177 | 99 | 106 | 0 | 1 | 0 | – | – | – | 58 | 104 | 63 | 66 | 110 | 81 |
Nucleus | 0 | 2 | 0 | 0 | 0 | 1 | – | 3 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | – | – | – | 0 | 1 | 0 | 0 | 2 | 0 |
Nuclear Periphery | 1 | 2 | 0 | 0 | 0 | 0 | – | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | – | – | 2 | 3 | 0 | 1 | 1 | 2 |
Nucleolus | 3 | 1 | 1 | 0 | 0 | 1 | – | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 1 | 0 | 0 | 0 |
Peroxisomes | 6 | 0 | 6 | 3 | 2 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | – | – | 2 | 2 | 1 | 0 | 2 | 1 |
SpindlePole | 27 | 21 | 46 | 9 | 11 | 1 | – | 2 | 3 | 7 | 1 | 8 | 17 | 17 | 11 | – | – | – | 19 | 38 | 12 | 13 | 22 | 17 |
Vac/Vac Membrane | 61 | 60 | 13 | 6 | 5 | 5 | – | 3 | 0 | 1 | 1 | 1 | 6 | 11 | 17 | – | – | – | 102 | 100 | 41 | 61 | 150 | 112 |
Unique Cell Count | 525 | 765 | 316 | 164 | 214 | 272 | 328 | 317 | 388 | 201 | 219 | 298 | 365 | 358 | 642 | 930 | 348 | 412 | 753 | 563 | ||||
Labelled Cell Count | 600 | 845 | 359 | 175 | 224 | 325 | 402 | 396 | 495 | 261 | 288 | 327 | 376 | 372 | 642 | 930 | 348 | 412 | 753 | 563 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.0 | 4.8 | 4.3 | 4.0 | 3.7 | 3.1 | – | 3.1 | 2.8 | 2.6 | 2.6 | 2.6 | 5.4 | 5.7 | 6.1 | 4.3 | 4.9 | – | 5.8 | 5.6 | 5.9 |
Std Deviation (1e-4) | 1.0 | 0.8 | 1.3 | 1.5 | 1.2 | 1.6 | – | 0.9 | 0.8 | 1.0 | 0.8 | 1.2 | 0.8 | 0.8 | 1.0 | 1.3 | 1.5 | – | 1.4 | 1.3 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.09 | -0.22 | -0.47 | – | -0.48 | -0.6 | -0.74 | -0.74 | -0.73 | 0.34 | 0.43 | 0.5 | 0.01 | 0.18 | – | 0.44 | 0.39 | 0.46 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.4973 | 1.2705 | 0.8017 | 0.7522 | 0.9211 | 1.1073 | 3.3038 | 3.7671 | 3.7976 | 3.8194 | 3.384 | 3.8835 | 4.4538 | 2.7358 | 2.1067 | 2.3253 | 2.6084 | 2.8623 |
Actin | 0.0038 | 0.0019 | 0.0033 | 0.0022 | 0.0209 | 0.001 | 0.0048 | 0.0018 | 0.0082 | 0.0123 | 0.0024 | 0.0055 | 0.0001 | 0.0016 | 0.0006 | 0.0001 | 0.0001 | 0.0047 |
Bud | 0.0007 | 0.0158 | 0.0037 | 0.0048 | 0.002 | 0.002 | 0.0077 | 0.0058 | 0.0044 | 0.0087 | 0.0086 | 0.0032 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Bud Neck | 0.0016 | 0.0011 | 0.0003 | 0.0009 | 0.0007 | 0.001 | 0.0021 | 0.0011 | 0.0023 | 0.0006 | 0.0017 | 0.0022 | 0.0001 | 0.0004 | 0.0001 | 0.0004 | 0.0002 | 0.001 |
Bud Periphery | 0.0006 | 0.0036 | 0.0015 | 0.0042 | 0.0019 | 0.0007 | 0.0047 | 0.0087 | 0.0038 | 0.0215 | 0.0042 | 0.0025 | 0.0001 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Bud Site | 0.003 | 0.0204 | 0.0016 | 0.0019 | 0.0011 | 0.0006 | 0.0075 | 0.0131 | 0.0096 | 0.0015 | 0.0017 | 0.0006 | 0.0009 | 0.0014 | 0.0003 | 0.0005 | 0.0001 | 0.0002 |
Cell Periphery | 0.0003 | 0.0005 | 0.0002 | 0.0002 | 0.0003 | 0.0002 | 0.0005 | 0.0005 | 0.0005 | 0.0002 | 0.0005 | 0.0003 | 0.0006 | 0.0003 | 0.0001 | 0 | 0.0001 | 0.0001 |
Cytoplasm | 0.1337 | 0.1671 | 0.1383 | 0.114 | 0.086 | 0.1717 | 0.0475 | 0.1087 | 0.0552 | 0.0442 | 0.0729 | 0.0725 | 0.0388 | 0.1326 | 0.0915 | 0.0728 | 0.2086 | 0.1113 |
Cytoplasmic Foci | 0.1835 | 0.1932 | 0.1983 | 0.2138 | 0.1894 | 0.2106 | 0.2164 | 0.238 | 0.2331 | 0.1639 | 0.2172 | 0.2762 | 0.0268 | 0.1734 | 0.2017 | 0.198 | 0.1698 | 0.2323 |
Eisosomes | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0005 | 0.0002 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.006 | 0.0049 | 0.005 | 0.0024 | 0.0038 | 0.0044 | 0.0039 | 0.0037 | 0.0022 | 0.0119 | 0.0037 | 0.0021 | 0.0034 | 0.0066 | 0.0054 | 0.0012 | 0.0056 | 0.0027 |
Endosome | 0.3961 | 0.3333 | 0.3674 | 0.4228 | 0.4349 | 0.3688 | 0.3527 | 0.3448 | 0.3015 | 0.3605 | 0.342 | 0.2777 | 0.052 | 0.3163 | 0.3892 | 0.2729 | 0.4106 | 0.3508 |
Golgi | 0.0195 | 0.015 | 0.0212 | 0.0156 | 0.0202 | 0.0221 | 0.0269 | 0.0169 | 0.0298 | 0.0285 | 0.0353 | 0.0191 | 0.0017 | 0.0075 | 0.0293 | 0.0158 | 0.0065 | 0.0246 |
Lipid Particles | 0.0318 | 0.0116 | 0.0174 | 0.0157 | 0.0206 | 0.0457 | 0.0636 | 0.0318 | 0.0345 | 0.0467 | 0.053 | 0.0532 | 0.0302 | 0.0264 | 0.009 | 0.0171 | 0.0049 | 0.0421 |
Mitochondria | 0.0052 | 0.0056 | 0.0064 | 0.0038 | 0.0126 | 0.0078 | 0.014 | 0.0082 | 0.0174 | 0.0131 | 0.0139 | 0.0069 | 0.0025 | 0.0022 | 0.0049 | 0.0005 | 0.001 | 0.0054 |
None | 0.1673 | 0.1769 | 0.1902 | 0.1416 | 0.1701 | 0.1281 | 0.1909 | 0.1438 | 0.2189 | 0.2328 | 0.139 | 0.2179 | 0.7826 | 0.293 | 0.2053 | 0.3928 | 0.162 | 0.1844 |
Nuclear Periphery | 0.0032 | 0.002 | 0.0027 | 0.0016 | 0.0043 | 0.0027 | 0.0053 | 0.0024 | 0.0018 | 0.006 | 0.0033 | 0.0015 | 0.0061 | 0.0034 | 0.0019 | 0.0006 | 0.0025 | 0.001 |
Nucleolus | 0.0006 | 0.0017 | 0.0006 | 0.0008 | 0.0007 | 0.0004 | 0.0013 | 0.003 | 0.0021 | 0.001 | 0.0009 | 0.0007 | 0.006 | 0.001 | 0.0002 | 0.0004 | 0.0002 | 0.0002 |
Nucleus | 0.0037 | 0.0057 | 0.002 | 0.002 | 0.0018 | 0.0015 | 0.0026 | 0.0043 | 0.0038 | 0.0019 | 0.0033 | 0.0013 | 0.0072 | 0.001 | 0.0005 | 0.0005 | 0.0007 | 0.0005 |
Peroxisomes | 0.0117 | 0.0064 | 0.0182 | 0.0066 | 0.0117 | 0.0117 | 0.0249 | 0.0279 | 0.0496 | 0.0228 | 0.0785 | 0.0402 | 0.0009 | 0.0079 | 0.044 | 0.0122 | 0.001 | 0.0129 |
Punctate Nuclear | 0.0054 | 0.0041 | 0.0022 | 0.0038 | 0.0048 | 0.002 | 0.005 | 0.004 | 0.0049 | 0.0035 | 0.0047 | 0.0043 | 0.0048 | 0.0014 | 0.0009 | 0.0043 | 0.001 | 0.0016 |
Vacuole | 0.0186 | 0.0247 | 0.0156 | 0.0334 | 0.0091 | 0.014 | 0.0141 | 0.0265 | 0.0134 | 0.0127 | 0.0092 | 0.0098 | 0.0313 | 0.0196 | 0.0099 | 0.0086 | 0.021 | 0.0208 |
Vacuole Periphery | 0.0034 | 0.0044 | 0.0041 | 0.0078 | 0.0028 | 0.003 | 0.0033 | 0.0047 | 0.0028 | 0.0056 | 0.0037 | 0.0023 | 0.0032 | 0.0031 | 0.0047 | 0.001 | 0.004 | 0.0032 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 19.5765 | 12.9396 | 18.1016 | 18.5898 | 16.1521 | 22.5302 | 21.0725 | 22.5823 | 22.1021 | 22.4936 |
Translational Efficiency | 0.2248 | 0.232 | 0.1987 | 0.1776 | 0.2314 | 0.1608 | 0.1852 | 0.2229 | 0.1187 | 0.1904 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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