Standard name
Human Ortholog
Description 3'-5' DNA helicase involved in error-free bypass of DNA lesions; binds flap DNA, stimulates activity of Rad27p and Dna2p; prevents crossovers between ectopic sequences by removing substrates for Mus81-Mms4 or Rad1-Rad10 cleavage; homolog of human FANCM Fanconi anemia protein that is involved in stabilizing and remodeling blocked replication forks; member of SF2 DExD/H superfamily of helicases; nonsense or missense mutations in FANCM can make people more likely to get cancer
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.07 0.17 0.2 0.21 0.24 0.2 0.2 0.23 0.18 – 0.2 0.17 0.18 0.26 0.29 0.18 0.15 0.2 0 0.07 0.06 0.05 0.06 0.09
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.16 0.44 0.56 0.38 0.67 0.71 0.65 0.82 – 0.82 0.83 0.07 0 0.22 0 0 0 0 0 0.05 0 0.05 0.05
Nucleus 0.82 0.67 0.47 0.5 0.47 0.38 0.36 0.39 0.29 – 0.22 0.23 0.69 0.63 0.38 0.68 0.66 0.61 0.72 0.68 0.51 0.56 0.41 0.25
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Vac/Vac Membrane 0.17 0.18 0.07 0 0.11 0.08 0.06 0 0.06 – 0.07 0.08 0.13 0.15 0.22 0.17 0.16 0.16 0.18 0.17 0.29 0.28 0.37 0.43
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 2 2 3 8 7 7 – 7 12 0 0 2 0 0 0 3 4 5 4 3 3
Bud Neck 0 0 1 0 0 1 1 0 0 – 0 0 0 0 0 0 0 0 0 1 0 2 2 2
Bud Site 0 0 0 2 4 4 10 13 9 – 10 14 0 0 1 0 0 0 – – – – – –
Cell Periphery 0 1 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 0 0 0 0 0 1
Cytoplasm 18 33 59 52 66 80 89 128 58 – 74 69 31 65 74 50 26 28 5 15 24 11 13 23
Endoplasmic Reticulum 3 0 0 0 1 0 0 0 0 – 0 1 0 0 0 11 12 5 0 0 1 1 0 2
Endosome 2 0 1 0 4 0 2 1 0 – 0 1 1 2 1 3 4 4 4 2 3 0 1 9
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 1 0 2 1 3 1 0 1 0 0 0
Mitochondria 0 32 132 139 105 276 320 358 267 – 306 343 12 6 57 8 4 4 5 5 19 2 10 14
Nucleus 220 134 142 124 127 157 162 213 94 – 81 96 120 157 99 192 114 85 223 156 196 125 97 67
Nuclear Periphery 1 0 0 0 0 0 2 1 0 – 0 1 0 0 0 1 0 0 0 0 1 1 0 0
Nucleolus 0 0 1 0 0 1 0 3 0 – 0 2 1 0 0 0 0 2 2 1 0 2 4 7
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 2 1 3 5 1 6 4 – 2 1 0 3 1 2 1 2 3 3 8 6 9 14
Vac/Vac Membrane 45 36 22 11 31 33 25 22 18 – 25 35 23 37 58 48 28 22 54 38 113 63 87 116
Unique Cell Count 268 199 301 247 273 410 449 549 324 372 414 173 249 258 284 172 140 309 230 386 226 236 271
Labelled Cell Count 289 236 360 331 343 560 620 752 457 505 575 188 271 293 318 190 155 309 230 386 226 236 271


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.6 4.1 3.7 3.7 3.9 3.4 3.3 3.4 2.8 – 2.8 2.7 4.6 4.7 4.0 6.3 6.8 7.3 4.8 4.7 5.1
Std Deviation (1e-4) 0.8 0.7 1.2 1.6 1.6 1.4 1.5 1.5 1.3 – 0.8 0.9 1.2 1.1 1.0 1.5 1.6 2.3 1.5 1.3 1.6
Intensity Change (Log2) – – – -0.01 0.06 -0.15 -0.18 -0.13 -0.4 – -0.42 -0.45 0.31 0.32 0.09 0.75 0.86 0.97 0.37 0.34 0.47


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 3.6 3.1 2.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.456 1.2714 1.2148 0.8313 0.9167 1.0516 2.0005 2.4442 2.3177 1.9071 2.8659 2.2121 2.7644 3.9394 2.9383 2.7882 2.6441 3.1586
Actin 0.064 0.0006 0.0161 0.0001 0.0136 0.0017 0.0169 0.0001 0.0224 0.0075 0.0254 0.0011 0.0034 0.0006 0.0056 0.0004 0.0005 0.0002
Bud 0.0009 0.0002 0.001 0.0002 0.0005 0.0016 0.0007 0.0002 0.0004 0.0011 0.0021 0.0001 0.0011 0.0001 0.0004 0.0001 0.0001 0
Bud Neck 0.0058 0.0009 0.0011 0.001 0.0015 0.0066 0.0022 0.0006 0.001 0.003 0.0036 0.0035 0.0003 0.0006 0.0022 0.0008 0.0017 0.0026
Bud Periphery 0.0022 0.0003 0.0018 0.0014 0.002 0.0047 0.0006 0.0002 0.0005 0.0012 0.0019 0.0001 0.0005 0.0001 0.0009 0.0001 0.0001 0
Bud Site 0.0048 0.0015 0.0169 0.0007 0.001 0.0011 0.0059 0.0015 0.0058 0.0115 0.0142 0.0002 0.0005 0.0005 0.0028 0.0002 0.0004 0.0001
Cell Periphery 0.0005 0.0002 0.0003 0.0006 0.0004 0.0002 0.0002 0.0001 0.0002 0.0004 0.0002 0 0.0001 0.0001 0.0001 0 0.0001 0
Cytoplasm 0.0384 0.0787 0.0401 0.0566 0.0298 0.0312 0.0521 0.0275 0.0313 0.0642 0.0207 0.0258 0.0633 0.0372 0.0467 0.0213 0.0486 0.0308
Cytoplasmic Foci 0.0158 0.004 0.007 0.0008 0.0023 0.0059 0.0195 0.001 0.0074 0.0335 0.0392 0.0024 0.0019 0.0021 0.0023 0.0003 0.0013 0.0008
Eisosomes 0.0011 0 0.0002 0 0.0007 0 0.0004 0 0.0002 0.0001 0.0003 0 0.0001 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0082 0.0035 0.0061 0.0017 0.0069 0.0024 0.0026 0.0018 0.0068 0.0027 0.0065 0.0012 0.003 0.0024 0.0018 0.0009 0.0026 0.0007
Endosome 0.0261 0.0034 0.0168 0.0059 0.0226 0.0072 0.0162 0.0021 0.0077 0.0272 0.021 0.0068 0.0024 0.0018 0.0053 0.0008 0.0027 0.0011
Golgi 0.0122 0.0002 0.0043 0.0001 0.0058 0.0018 0.0045 0.0001 0.0035 0.0078 0.0206 0.0019 0.001 0.0001 0.0009 0 0.0001 0
Lipid Particles 0.0297 0.0009 0.013 0.0005 0.0061 0.0085 0.0068 0.0007 0.0025 0.017 0.0063 0.0028 0.0026 0.0002 0.0022 0 0.0002 0.0001
Mitochondria 0.0124 0.0007 0.0085 0.0017 0.0275 0.0013 0.0029 0.0008 0.0033 0.0049 0.0076 0.0034 0.0014 0.0004 0.0014 0.0011 0.0005 0.0003
None 0.0915 0.0469 0.0662 0.0294 0.0256 0.038 0.1181 0.0844 0.0668 0.0749 0.1181 0.0761 0.1005 0.0558 0.0817 0.0384 0.0621 0.0662
Nuclear Periphery 0.0334 0.0209 0.0253 0.0098 0.0375 0.0152 0.0189 0.0165 0.024 0.0152 0.0159 0.013 0.0104 0.0272 0.0178 0.0123 0.0259 0.0066
Nucleolus 0.0085 0.0101 0.008 0.0082 0.0086 0.017 0.0099 0.0124 0.007 0.0081 0.0065 0.0167 0.0045 0.0069 0.0101 0.0074 0.0093 0.0132
Nucleus 0.5697 0.7982 0.732 0.8188 0.7692 0.8178 0.6452 0.817 0.7691 0.5838 0.6272 0.8006 0.7888 0.8045 0.779 0.8483 0.8114 0.8218
Peroxisomes 0.023 0.0003 0.006 0.0001 0.0014 0.0027 0.0071 0.0001 0.0059 0.0516 0.0099 0.0027 0.0004 0.0001 0.0033 0.0001 0.0001 0
Punctate Nuclear 0.0441 0.0217 0.021 0.0314 0.0316 0.0329 0.0655 0.0299 0.0322 0.0744 0.0509 0.0404 0.0116 0.057 0.0268 0.0668 0.0283 0.0545
Vacuole 0.0057 0.0059 0.0057 0.0265 0.0027 0.0018 0.0027 0.0022 0.0016 0.0087 0.0012 0.0008 0.0019 0.0019 0.0057 0.0004 0.0035 0.0007
Vacuole Periphery 0.0021 0.0007 0.0024 0.0045 0.0029 0.0004 0.001 0.0006 0.0006 0.0012 0.0008 0.0003 0.0003 0.0004 0.0029 0.0002 0.0007 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.0304 9.9233 7.1181 9.6405 9.3551 5.9171 9.8662 8.9163 8.8961 9.6984
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –
Standard name
Human Ortholog
Description 3'-5' DNA helicase involved in error-free bypass of DNA lesions; binds flap DNA, stimulates activity of Rad27p and Dna2p; prevents crossovers between ectopic sequences by removing substrates for Mus81-Mms4 or Rad1-Rad10 cleavage; homolog of human FANCM Fanconi anemia protein that is involved in stabilizing and remodeling blocked replication forks; member of SF2 DExD/H superfamily of helicases; nonsense or missense mutations in FANCM can make people more likely to get cancer
Localization
Cell Percentages nucleus (45%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Mph1

Mph1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mph1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available