Standard name | |
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Human Ortholog | |
Description | Basic leucine zipper (bZIP) iron-sensing transcription factor; involved in diauxic shift; YAP5 has a paralog, YAP7, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 1 | 2 | 2 | 1 | 1 | 4 |
Bud | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 3 | 3 | 3 | 6 | 7 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 1 | 4 |
Bud Neck | 1 | 1 | 4 | 3 | 2 | 5 | 7 | 11 | 2 | 3 | 1 | 4 | 0 | 1 | 0 | – | – | – | 0 | 0 | 0 | 0 | 2 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | – | – | – | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 0 | 0 | 2 | 2 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | – | – | – | 0 | 1 | 0 | 0 | 0 | 0 |
Cytoplasm | 92 | 135 | 153 | 127 | 161 | 245 | 271 | 368 | 94 | 164 | 141 | 126 | 97 | 284 | 399 | – | – | – | 89 | 137 | 113 | 168 | 216 | 268 |
Endoplasmic Reticulum | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 3 | 1 | 1 | – | – | – | 0 | 0 | 1 | 0 | 2 | 1 |
Endosome | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 0 | – | – | – | 2 | 1 | 2 | 1 | 1 | 4 |
Golgi | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 2 | 0 | 0 | 0 |
Mitochondria | 2 | 27 | 0 | 11 | 5 | 13 | 24 | 40 | 93 | 111 | 118 | 119 | 1 | 2 | 1 | – | – | – | 13 | 10 | 24 | 9 | 7 | 13 |
Nucleus | 0 | 0 | 0 | 2 | 1 | 3 | 8 | 5 | 4 | 7 | 5 | 7 | 22 | 7 | 1 | – | – | – | 2 | 2 | 2 | 4 | 2 | 1 |
Nuclear Periphery | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 1 | 0 | 1 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 1 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 1 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 7 | 3 | 9 | 5 | 4 | 0 | 2 | 1 | – | – | – | 2 | 3 | 5 | 3 | 4 | 7 |
Vac/Vac Membrane | 9 | 1 | 11 | 15 | 8 | 8 | 8 | 10 | 5 | 10 | 7 | 13 | 18 | 14 | 6 | – | – | – | 16 | 6 | 15 | 17 | 9 | 10 |
Unique Cell Count | 96 | 157 | 165 | 149 | 175 | 258 | 301 | 415 | 173 | 252 | 238 | 237 | 134 | 301 | 403 | 133 | 174 | 184 | 214 | 260 | 333 | |||
Labelled Cell Count | 104 | 167 | 173 | 163 | 179 | 279 | 321 | 445 | 207 | 310 | 286 | 281 | 143 | 312 | 411 | 133 | 174 | 184 | 214 | 260 | 333 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.9 | 3.0 | 5.0 | 4.8 | 5.4 | 4.3 | 4.0 | 4.3 | 3.4 | 3.7 | 3.5 | 3.5 | 4.6 | 4.8 | 4.7 | 4.0 | 4.4 | 4.5 | 5.1 | 5.6 | 5.3 |
Std Deviation (1e-4) | 0.3 | 0.5 | 1.4 | 1.4 | 2.4 | 1.6 | 1.2 | 1.2 | 1.3 | 1.3 | 0.9 | 0.7 | 0.7 | 1.0 | 1.1 | 1.6 | 1.0 | 1.2 | 1.2 | 1.8 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.06 | 0.11 | -0.24 | -0.32 | -0.23 | -0.57 | -0.45 | -0.51 | -0.53 | -0.14 | -0.05 | -0.11 | -0.33 | -0.2 | -0.16 | 0.02 | 0.15 | 0.08 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.806 | -0.3655 | -0.7313 | -0.9915 | -0.5533 | -0.7207 | 2.2766 | 2.2606 | 2.1556 | 2.5652 | 1.8365 | 2.2733 | -1.4007 | -0.8121 | -0.9491 | -0.6249 | -1.0972 | -0.9236 |
Actin | 0.0014 | 0.0007 | 0.0005 | 0.0002 | 0.024 | 0.0035 | 0.0339 | 0.0003 | 0.0024 | 0.0007 | 0.0137 | 0.0031 | 0.024 | 0.0003 | 0.0064 | 0.0083 | 0.0275 | 0.0008 |
Bud | 0.0004 | 0.0035 | 0.0022 | 0 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0008 | 0.0003 | 0.0006 | 0.0006 | 0.0002 | 0.0004 |
Bud Neck | 0.0009 | 0.0005 | 0.0009 | 0.0006 | 0.0029 | 0.0049 | 0.0019 | 0.0002 | 0.0003 | 0.001 | 0.0007 | 0.0017 | 0.0021 | 0.0002 | 0.0014 | 0.0012 | 0.001 | 0.0043 |
Bud Periphery | 0.0005 | 0.0006 | 0.0003 | 0 | 0.0002 | 0.0006 | 0.0008 | 0 | 0.0004 | 0.0001 | 0.0004 | 0.0002 | 0.0013 | 0.0002 | 0.0011 | 0.0019 | 0.0004 | 0.0002 |
Bud Site | 0.0055 | 0.0037 | 0.0033 | 0.0001 | 0.0004 | 0.0001 | 0.0017 | 0.0006 | 0.0006 | 0.0005 | 0.0005 | 0.0001 | 0.0132 | 0.0031 | 0.0027 | 0.0007 | 0.0005 | 0.0002 |
Cell Periphery | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0044 | 0 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0 |
Cytoplasm | 0.1746 | 0.1175 | 0.1493 | 0.2378 | 0.0934 | 0.1509 | 0.1339 | 0.2566 | 0.1732 | 0.2018 | 0.1494 | 0.2614 | 0.2754 | 0.2842 | 0.237 | 0.2692 | 0.3978 | 0.3698 |
Cytoplasmic Foci | 0.0149 | 0.0162 | 0.0073 | 0.0074 | 0.0092 | 0.0142 | 0.0197 | 0.0077 | 0.0085 | 0.004 | 0.0092 | 0.0121 | 0.017 | 0.0097 | 0.0061 | 0.0027 | 0.0085 | 0.0139 |
Eisosomes | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0013 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0002 | 0.0002 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.0042 | 0.0025 | 0.0035 | 0.0012 | 0.0017 | 0.002 | 0.0074 | 0.0024 | 0.0025 | 0.0046 | 0.0015 | 0.0022 | 0.0055 | 0.0077 | 0.0051 | 0.0044 | 0.0044 | 0.0044 |
Endosome | 0.0062 | 0.0029 | 0.0034 | 0.0025 | 0.0042 | 0.0032 | 0.0083 | 0.0025 | 0.002 | 0.0026 | 0.004 | 0.0042 | 0.0104 | 0.0138 | 0.0094 | 0.0189 | 0.0155 | 0.0049 |
Golgi | 0.0005 | 0.0002 | 0.0001 | 0.0001 | 0.0024 | 0.0002 | 0.0034 | 0.0001 | 0.0002 | 0.0001 | 0.0021 | 0.0005 | 0.0044 | 0.0022 | 0.0032 | 0.0061 | 0.0234 | 0.0004 |
Lipid Particles | 0.0036 | 0.0008 | 0.0005 | 0.0002 | 0.0021 | 0.001 | 0.008 | 0.0002 | 0.0005 | 0.0003 | 0.0118 | 0.0018 | 0.0076 | 0.0005 | 0.0059 | 0.0021 | 0.0416 | 0.0021 |
Mitochondria | 0.0009 | 0.001 | 0.0007 | 0.0008 | 0.0007 | 0.0007 | 0.0017 | 0.0006 | 0.0007 | 0.0008 | 0.0027 | 0.0007 | 0.0053 | 0.001 | 0.0062 | 0.0083 | 0.0062 | 0.0006 |
None | 0.5322 | 0.7312 | 0.6036 | 0.5866 | 0.7274 | 0.5191 | 0.6827 | 0.6279 | 0.7109 | 0.6071 | 0.6685 | 0.5288 | 0.4658 | 0.5704 | 0.5665 | 0.4016 | 0.3963 | 0.3476 |
Nuclear Periphery | 0.0153 | 0.0066 | 0.0195 | 0.0076 | 0.007 | 0.0118 | 0.0134 | 0.0053 | 0.0049 | 0.0129 | 0.0095 | 0.0092 | 0.0146 | 0.0078 | 0.0107 | 0.0117 | 0.0063 | 0.0084 |
Nucleolus | 0.0034 | 0.0027 | 0.0034 | 0.0013 | 0.002 | 0.0047 | 0.001 | 0.001 | 0.0012 | 0.0013 | 0.0019 | 0.0014 | 0.0019 | 0.001 | 0.0007 | 0.0013 | 0.0003 | 0.002 |
Nucleus | 0.1963 | 0.0566 | 0.1549 | 0.1068 | 0.0919 | 0.1972 | 0.0494 | 0.075 | 0.0692 | 0.1445 | 0.0846 | 0.1281 | 0.1185 | 0.0838 | 0.1233 | 0.248 | 0.0566 | 0.1993 |
Peroxisomes | 0.0018 | 0.0012 | 0.0006 | 0.0002 | 0.0045 | 0.0004 | 0.0124 | 0.0002 | 0.0028 | 0.0003 | 0.0154 | 0.002 | 0.0067 | 0.0001 | 0.0008 | 0.0002 | 0.0036 | 0.0036 |
Punctate Nuclear | 0.0349 | 0.0502 | 0.0437 | 0.0456 | 0.0247 | 0.0843 | 0.0123 | 0.0176 | 0.0179 | 0.0161 | 0.0224 | 0.041 | 0.0189 | 0.01 | 0.0079 | 0.004 | 0.0073 | 0.0346 |
Vacuole | 0.0018 | 0.0011 | 0.0015 | 0.0006 | 0.0006 | 0.0007 | 0.0017 | 0.0012 | 0.0012 | 0.001 | 0.0008 | 0.0008 | 0.005 | 0.0031 | 0.0032 | 0.005 | 0.0014 | 0.0021 |
Vacuole Periphery | 0.0006 | 0.0002 | 0.0004 | 0.0002 | 0.0003 | 0.0002 | 0.0006 | 0.0002 | 0.0002 | 0.0003 | 0.0004 | 0.0003 | 0.0012 | 0.0005 | 0.0012 | 0.0033 | 0.0009 | 0.0003 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 12.7086 | 9.3559 | 18.8438 | 18.8725 | 19.385 | 32.8746 | 25.0007 | 37.3959 | 33.3274 | 24.8004 |
Translational Efficiency | 1.5857 | 2.3147 | 1.0666 | 0.9319 | 0.7005 | 0.9133 | 0.9594 | 0.7882 | 0.8741 | 0.8912 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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