Standard name
Human Ortholog
Description Splicing protein; required for splicing of two mitochondrial group I introns (BI3 in COB and AI5beta in COX1); forms a splicing complex, containing four subunits of Mrs1p and two subunits of the BI3-encoded maturase, that binds to the BI3 RNA; MRS1 has a paralog, CCE1, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.13 0 0 0 0 0 0 0 0 0 0.09 0 0 0 0.07 0 0 0 0 0 0 0
Bud 0 0 0.09 0.05 0 0 0.05 0.06 0.08 0 0.08 0.06 0 0 0 0.07 0 0.07 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.09 0.1 0 0 0 0 0 0
Cytoplasm 0 0.13 0.24 0.19 0.2 0.19 0.17 0.17 0.17 0.21 0.12 0.18 0.3 0.43 0.5 0.12 0.1 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0.05 0.05 0.07 0 0 0 0 0 0 0 0 0 0.05 0 0 0.06 0 0 0 0 0 0 0 0
Endosome 0 0 0 0.06 0.19 0 0 0 0 0 0 0 0.11 0.16 0.18 0.19 0.18 0.24 0.09 0.08 0.1 0.1 0.09 0.08
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.18 0.06 0 0 0 0 0 0
Mitochondria 0.94 0.84 0.43 0.58 0.47 0.69 0.79 0.73 0.81 0.76 0.88 0.78 0.36 0.17 0.1 0.22 0.1 0.07 0.7 0.72 0.73 0.68 0.63 0.65
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0.1 0.12 0 0 0 0 0 0 0 0 0.12 0.18 0.09 0.09 0.11 0.06 0 0 0 0.06 0.07
SpindlePole 0 0 0.14 0.19 0.17 0.11 0.11 0.17 0.13 0.08 0.08 0.1 0.09 0.08 0 0.14 0.13 0.1 0 0 0 0 0 0
Vac/Vac Membrane 0 0.09 0.05 0 0.13 0.07 0 0 0 0 0 0 0.1 0.09 0.12 0.17 0.19 0.2 0 0.06 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 4 14 6 0 0 0 1 0 0 0 0 19 8 0 5 5 3 1 3 2 6 9 13
Bud 5 5 10 13 15 14 29 33 37 19 35 23 7 6 1 10 2 5 0 0 0 1 5 5
Bud Neck 0 0 3 4 6 4 4 10 4 6 4 7 3 3 1 0 0 0 1 0 0 2 7 4
Bud Site 0 0 0 2 2 2 7 5 6 10 6 7 0 0 0 0 2 0
Cell Periphery 0 5 2 4 8 1 2 1 3 4 1 2 7 9 6 12 6 7 0 0 0 0 1 0
Cytoplasm 11 21 26 55 91 69 94 87 76 93 53 74 66 103 163 16 7 5 3 3 3 3 10 8
Endoplasmic Reticulum 12 8 7 1 0 6 0 0 1 1 1 0 10 6 3 8 3 3 2 0 0 0 3 1
Endosome 2 3 3 17 87 10 10 6 2 4 0 3 24 38 59 27 12 17 24 14 16 19 36 29
Golgi 1 1 2 1 1 0 0 0 0 0 0 1 2 0 1 11 12 4 6 1 1 1 7 5
Mitochondria 247 134 46 166 216 253 447 374 372 340 379 326 79 40 33 30 7 5 189 132 125 128 246 250
Nucleus 2 3 1 6 2 6 11 11 6 9 8 13 1 2 0 4 1 5 0 1 0 0 0 1
Nuclear Periphery 6 1 1 0 0 2 4 0 3 1 1 2 1 0 0 0 0 1 7 1 1 0 2 1
Nucleolus 3 0 0 0 0 1 1 5 4 1 2 2 0 0 0 0 0 0 0 1 0 0 3 3
Peroxisomes 1 1 0 27 54 6 7 8 8 5 2 8 5 28 59 13 6 8 16 7 6 8 22 26
SpindlePole 8 5 15 53 78 40 61 88 59 38 35 43 20 18 5 20 9 7 5 2 1 4 12 11
Vac/Vac Membrane 5 15 5 6 61 24 25 13 7 12 14 15 21 22 40 24 13 14 5 10 5 6 14 18
Unique Cell Count 262 160 107 284 455 365 568 513 458 450 433 418 220 237 323 139 68 71 270 184 172 189 394 388
Labelled Cell Count 304 206 135 361 621 438 702 642 588 543 541 526 265 283 371 180 85 84 270 184 172 189 394 388


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.8 4.8 5.2 4.8 5.0 4.0 3.8 4.2 3.5 3.6 3.2 3.7 5.2 5.4 5.3 6.2 7.2 6.7 4.8 5.2 5.5
Std Deviation (1e-4) 0.6 0.8 1.3 1.3 1.5 1.2 1.6 1.3 1.0 1.1 1.1 1.3 0.9 1.1 1.0 1.3 1.7 1.4 1.0 1.0 1.2
Intensity Change (Log2) -0.13 -0.05 -0.38 -0.45 -0.32 -0.59 -0.53 -0.69 -0.49 0.0 0.05 0.02 0.25 0.46 0.35 -0.12 0.0 0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 3.0 3.1 3.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.9287 1.1258 0.7818 0.436 0.7128 0.9386 -0.2374 0.5809 0.3429 -0.0046 -0.3815 0.3716 -0.2632 -0.3122 -0.4942 -0.1969 -0.3237 -0.4239
Actin 0.0264 0.0173 0.0073 0.0053 0.0319 0.0077 0.0278 0.0112 0.017 0.0097 0.051 0.0177 0.0433 0.0247 0.0113 0.0284 0.1208 0.0151
Bud 0.0019 0.0061 0.0027 0.0008 0.0039 0.0017 0.0014 0.0018 0.0012 0.0005 0.0149 0.0008 0.0025 0.0032 0.0039 0.0042 0.0027 0.0016
Bud Neck 0.0021 0.0009 0.0026 0.0022 0.0015 0.0048 0.0021 0.0028 0.0024 0.0029 0.0015 0.0087 0.0028 0.0014 0.0027 0.0024 0.0008 0.0121
Bud Periphery 0.0038 0.0134 0.0095 0.002 0.0062 0.004 0.0036 0.003 0.0038 0.0008 0.0332 0.002 0.0059 0.0049 0.0088 0.0094 0.0046 0.0054
Bud Site 0.0097 0.0166 0.0146 0.0019 0.0045 0.0009 0.0064 0.0268 0.0101 0.001 0.0112 0.0012 0.009 0.0356 0.0112 0.0079 0.0025 0.0036
Cell Periphery 0.0012 0.0008 0.0035 0.0008 0.0011 0.0005 0.002 0.0022 0.0007 0.0002 0.0008 0.0007 0.0015 0.0019 0.001 0.001 0.0002 0.0014
Cytoplasm 0.0132 0.0206 0.0403 0.0111 0.0403 0.0275 0.0355 0.0697 0.0366 0.0557 0.024 0.0679 0.0402 0.073 0.0507 0.0659 0.0182 0.0417
Cytoplasmic Foci 0.1274 0.1349 0.09 0.109 0.1206 0.106 0.0963 0.1071 0.0781 0.0755 0.1007 0.1193 0.1239 0.1304 0.1075 0.1287 0.0716 0.086
Eisosomes 0.0017 0.0007 0.0012 0.0004 0.0008 0.0003 0.001 0.0009 0.0006 0.0001 0.0008 0.0004 0.002 0.001 0.0011 0.0011 0.0003 0.0007
Endoplasmic Reticulum 0.0034 0.0017 0.0023 0.0057 0.0014 0.0013 0.0059 0.0068 0.0057 0.0098 0.0025 0.0051 0.0081 0.0051 0.0045 0.0046 0.0011 0.0031
Endosome 0.1228 0.1322 0.0846 0.1218 0.1067 0.0856 0.1418 0.1349 0.0911 0.1165 0.0741 0.1137 0.2016 0.1542 0.1219 0.1176 0.1929 0.1241
Golgi 0.0739 0.1044 0.0477 0.0983 0.0809 0.0654 0.0649 0.0869 0.0406 0.0543 0.0315 0.0618 0.0854 0.0876 0.0665 0.0632 0.1186 0.0554
Lipid Particles 0.0716 0.0126 0.0261 0.0136 0.0371 0.041 0.0752 0.0216 0.0129 0.0122 0.0104 0.0286 0.0554 0.0227 0.03 0.0146 0.0639 0.0332
Mitochondria 0.3177 0.3598 0.4517 0.5012 0.33 0.4172 0.3315 0.2949 0.4772 0.4927 0.4477 0.3458 0.1998 0.2862 0.3532 0.3175 0.226 0.4002
None 0.0897 0.0736 0.0965 0.0416 0.1286 0.1006 0.1181 0.1606 0.1502 0.111 0.0788 0.1306 0.1298 0.0981 0.1527 0.1399 0.0479 0.1375
Nuclear Periphery 0.0043 0.0007 0.0009 0.0005 0.001 0.0012 0.0039 0.0016 0.0024 0.0102 0.0143 0.0016 0.0096 0.0019 0.0087 0.0016 0.0009 0.0011
Nucleolus 0.0016 0.0006 0.0009 0.0002 0.0065 0.0007 0.0016 0.0012 0.0007 0.0006 0.0016 0.0025 0.0012 0.0006 0.0008 0.0006 0.0003 0.0005
Nucleus 0.0012 0.0007 0.0012 0.0003 0.0019 0.001 0.0019 0.0018 0.0012 0.0033 0.004 0.0013 0.0039 0.001 0.0015 0.001 0.0005 0.0007
Peroxisomes 0.1121 0.0941 0.1069 0.0754 0.0824 0.122 0.0613 0.048 0.0525 0.0229 0.0812 0.078 0.0389 0.0512 0.0426 0.0703 0.1126 0.0623
Punctate Nuclear 0.0022 0.0007 0.0016 0.0007 0.0041 0.0025 0.003 0.0025 0.0024 0.0028 0.0064 0.0044 0.0077 0.0017 0.0037 0.0009 0.0005 0.001
Vacuole 0.0091 0.0052 0.0054 0.0041 0.0063 0.0054 0.0103 0.01 0.0078 0.009 0.0054 0.005 0.019 0.0098 0.0101 0.0113 0.0091 0.007
Vacuole Periphery 0.003 0.0025 0.0025 0.0029 0.0023 0.0029 0.0042 0.0036 0.0048 0.0084 0.0038 0.0029 0.0085 0.0039 0.0055 0.0077 0.0039 0.0061

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.1253 6.3229 16.0865 17.6566 11.7908 7.0692 16.8961 14.4858 17.0535 16.707
Translational Efficiency 0.7764 1.039 0.3805 0.6527 0.6846 1.2653 0.603 0.5702 0.6184 0.6874

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Splicing protein; required for splicing of two mitochondrial group I introns (BI3 in COB and AI5beta in COX1); forms a splicing complex, containing four subunits of Mrs1p and two subunits of the BI3-encoded maturase, that binds to the BI3 RNA; MRS1 has a paralog, CCE1, that arose from the whole genome duplication
Localization
Cell Percentages mitochondrion (86%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Mrs1

Mrs1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mrs1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available