Standard name
Human Ortholog
Description Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.06 0.06 0.05 0.05 0.09 0 – 0 0 0.07 0.13 0.16 0.08 0.08 0.13 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.09 0.09 0.06 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.09 0.16 0.2 0.06 0.3 0.25 0.31 0.51 – 0.52 0.57 0.07 0 0.13 0.09 0.05 0.12 0 0 0 0 0 0
Nucleus 0.69 0.81 0.75 0.79 0.82 0.67 0.63 0.63 0.57 – 0.58 0.45 0.8 0.77 0.7 0.67 0.65 0.66 0.51 0.7 0.53 0.74 0.59 0.52
Nuclear Periphery 0 0 0 0 0 0 0.05 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.39 0.21 0.25 0.15 0.15 0.26 0.26 0.24 0.31 – 0.35 0.33 0.18 0.17 0.16 0.11 0.11 0.09 0.34 0.16 0.3 0.15 0.24 0.24
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.1 0.15 0.11 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.06 0.05 0.05 0 0 0 0 0.07 0.11
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 0 0 1 0 0 0
Bud 0 0 0 0 0 2 1 6 4 – 5 3 1 2 0 1 1 1 0 2 0 3 2 4
Bud Neck 0 0 0 0 0 0 0 0 0 – 2 2 0 0 0 0 0 0 0 0 0 0 1 1
Bud Site 1 0 2 0 1 6 3 18 16 – 14 21 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 1 0 0 0 0 0 0
Cytoplasm 1 3 7 16 20 17 23 42 13 – 16 15 14 34 47 35 26 39 0 1 1 1 0 3
Endoplasmic Reticulum 0 0 0 0 0 0 1 1 0 – 0 0 0 1 0 16 7 11 0 0 4 0 1 1
Endosome 4 0 3 1 4 0 2 2 0 – 0 1 3 0 1 39 28 18 7 3 6 3 9 9
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 2 1 1 0 1
Mitochondria 6 16 39 54 21 101 111 150 194 – 192 260 15 12 39 42 14 36 2 2 1 0 3 5
Nucleus 148 143 182 216 281 223 280 303 214 – 217 207 165 208 206 301 202 202 129 140 141 159 149 150
Nuclear Periphery 4 2 3 3 13 13 21 10 10 – 5 15 1 2 6 2 2 5 5 0 2 0 0 2
Nucleolus 84 37 61 42 53 86 116 116 118 – 128 150 38 45 48 50 34 29 87 32 80 32 59 69
Peroxisomes 0 0 0 0 0 0 1 0 0 – 0 1 0 0 1 0 2 0 0 0 0 0 0 0
SpindlePole 5 0 9 1 0 4 2 4 8 – 0 2 3 3 4 47 47 33 11 2 11 4 4 6
Vac/Vac Membrane 0 4 1 2 3 2 2 4 1 – 1 6 0 2 5 27 15 15 9 8 11 5 16 30
Unique Cell Count 214 177 244 273 342 334 441 480 377 371 455 206 270 294 452 309 308 254 200 267 215 253 290
Labelled Cell Count 253 205 307 335 396 454 563 656 578 580 683 240 309 357 560 379 391 254 200 267 215 253 290


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.4 6.3 5.6 5.5 6.0 5.2 5.2 5.0 4.2 – 4.2 4.1 6.1 6.2 5.4 6.2 6.7 6.4 6.8 6.9 7.5
Std Deviation (1e-4) 1.1 1.1 1.1 1.5 1.6 1.7 2.0 1.7 1.3 – 1.1 1.2 1.8 1.6 1.5 1.2 1.4 1.4 1.8 1.8 2.2
Intensity Change (Log2) – – – -0.0 0.11 -0.08 -0.1 -0.15 -0.39 – -0.39 -0.44 0.12 0.15 -0.03 0.15 0.28 0.2 0.3 0.32 0.43


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 2.3 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 1.6 1.7 1.3 1.4 3.0 0.4 – 0.9 0.3 2.0 4.1 5.0 2.6 2.7 4.1
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 3.0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 3.9 4.0 2.8
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 1.1 -3.9 4.0 2.8 4.4 0 – 0 0 -2.8 -4.4 -0.9 -2.6 -4.5 -1.5
Nucleus 1.2 2.2 -2.0 -3.0 -3.1 -4.5 – -4.1 -7.4 1.4 0.6 -1.2 -2.2 -2.3 -2.3
Nuclear Periphery 0 1.9 1.9 2.4 0 0 – 0 0 0 0 0 0 0 0
Nucleolus -2.7 -2.9 0.2 0.4 -0.2 0 – 0 0 -1.7 -2.3 -2.5 -4.8 -4.3 -4.9
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 -2.0 0 0 0 – 0 0 -1.5 -1.9 -1.8 3.1 4.5 3.1
Vacuole 0 0 0 0 0 0 – 0 0 0 0 0 3.6 3.1 3.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.5444 3.2557 3.3545 2.5495 2.5285 3.1181 4.003 4.4011 3.6058 4.0534 3.9993 3.722 5.1294 4.8849 4.7677 4.5906 4.2241 4.6452
Actin 0.0558 0.0005 0.0142 0.0004 0.0265 0.0013 0.0265 0.0001 0.015 0.0327 0.0314 0.0007 0.0023 0 0.0167 0.0006 0.0003 0.0021
Bud 0.0005 0.0001 0.0005 0.0001 0.0002 0.0001 0.0006 0.0001 0.0005 0.0004 0.0004 0.0001 0.0006 0 0.0002 0.0001 0 0.0002
Bud Neck 0.002 0.0013 0.0026 0.0004 0.0026 0.0017 0.0024 0.0008 0.0043 0.0011 0.0011 0.0011 0.0025 0.0002 0.0023 0.0003 0.0007 0.0016
Bud Periphery 0.0009 0.0002 0.001 0.0003 0.0006 0.0003 0.0018 0.0003 0.0013 0.0011 0.0021 0.0003 0.0017 0 0.0003 0.0002 0.0001 0.0007
Bud Site 0.0047 0.0079 0.0072 0.0002 0.0008 0.0003 0.0021 0.0051 0.0064 0.0083 0.002 0.0002 0.0046 0 0.0046 0.0002 0.0002 0.0003
Cell Periphery 0.0003 0.0001 0.0004 0 0.0001 0.0001 0.0004 0.0001 0.0004 0.0022 0.0009 0 0.0004 0 0.0006 0 0 0.0001
Cytoplasm 0.0053 0.0001 0.001 0.0002 0.0008 0.0001 0.0114 0.0001 0.0032 0.0012 0.0142 0.0003 0.0019 0.0002 0.0022 0.0006 0.0002 0.0003
Cytoplasmic Foci 0.0127 0.0002 0.0059 0.0001 0.0046 0.0003 0.0222 0.0002 0.0235 0.0148 0.0067 0.0006 0.0072 0 0.0092 0.0001 0.004 0.0012
Eisosomes 0.0007 0 0.0003 0 0.0002 0 0.0004 0 0.0003 0.0011 0.0003 0 0.0001 0 0.0001 0 0 0
Endoplasmic Reticulum 0.003 0 0.0032 0.0001 0.002 0.0001 0.0073 0 0.0027 0.0009 0.0018 0.0006 0.0021 0.0001 0.003 0.0002 0.0002 0.0006
Endosome 0.023 0.0001 0.0083 0.0002 0.0154 0.0006 0.0377 0.0002 0.0132 0.0408 0.0517 0.0036 0.0138 0.0001 0.0098 0.0006 0.0064 0.0052
Golgi 0.0094 0.0001 0.0052 0 0.0075 0.0006 0.0098 0.0001 0.0074 0.0107 0.0068 0.0005 0.0077 0 0.005 0.0001 0.0011 0.0025
Lipid Particles 0.0215 0.0002 0.0151 0.0001 0.0216 0.0038 0.024 0.0002 0.0131 0.0117 0.008 0.0029 0.01 0 0.0106 0.0002 0.0306 0.0023
Mitochondria 0.0071 0.0007 0.0246 0.0006 0.0024 0.0032 0.0189 0.0003 0.0123 0.0152 0.0357 0.003 0.0076 0.0003 0.0041 0.0033 0.0003 0.0066
None 0.0061 0.0001 0.0006 0.0002 0.0013 0.0001 0.0096 0.0001 0.0016 0.0005 0.0087 0.0002 0.0037 0 0.0024 0.0006 0.0001 0.0002
Nuclear Periphery 0.0186 0.0035 0.0175 0.0185 0.0147 0.0015 0.0283 0.0079 0.005 0.0291 0.0178 0.0029 0.0106 0.0077 0.0076 0.0258 0.0262 0.0083
Nucleolus 0.0904 0.0888 0.0734 0.0439 0.2284 0.1351 0.0808 0.0691 0.0589 0.0454 0.1831 0.1197 0.0711 0.0813 0.0704 0.0691 0.1388 0.1517
Nucleus 0.6794 0.8765 0.7944 0.8907 0.6423 0.8453 0.674 0.8863 0.7827 0.7728 0.566 0.8543 0.8221 0.9024 0.8267 0.8525 0.7116 0.8063
Peroxisomes 0.0123 0.0001 0.0121 0.0001 0.002 0.001 0.0139 0.0001 0.0222 0.0016 0.0034 0.0004 0.0043 0 0.0019 0.0001 0.0008 0.0007
Punctate Nuclear 0.0413 0.019 0.0086 0.0433 0.0246 0.004 0.0209 0.0281 0.0226 0.005 0.0322 0.0079 0.0184 0.0073 0.0204 0.0446 0.0778 0.0076
Vacuole 0.0036 0.0002 0.0023 0.0003 0.0006 0.0002 0.0036 0.0005 0.0024 0.0024 0.0141 0.0004 0.0043 0.0001 0.0015 0.0003 0.0002 0.0007
Vacuole Periphery 0.0016 0.0001 0.0017 0.0003 0.0007 0.0002 0.0035 0.0003 0.001 0.0009 0.0115 0.0003 0.0028 0.0001 0.0005 0.0006 0.0003 0.0008

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.4469 14.5603 11.0556 15.9944 9.5767 10.9787 16.7761 18.6017 13.7507 18.8493
Translational Efficiency 0.5651 0.5951 0.6187 0.3991 0.7718 0.7251 0.5748 0.4684 0.5527 0.4531

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
Localization
Cell Percentages nucleus (92%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Dal81

Dal81


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dal81-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available