Standard name
Human Ortholog
Description Putative cytoplasmic short-chain dehydrogenase/reductase
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.94 – 1.0 0.96 0.87 0.87 0.73 0.78 0.65 0.55 0.98 0.99 1.0 0.97 0.93 0.95 0.93 0.96 0.93 0.87 0.92 0.87
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 – 0.05 0 0.06 0.08 0.39 0.33 0.41 0.42 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0.06 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 – 0 0 0.1 0.1 0 0 0.05 0.13 0 0 0 0 0.07 0.08 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 1 3
Bud 0 0 0 – 0 0 0 1 2 0 2 1 0 0 0 0 0 0 3 4 0 2 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 1 0 – 0 1 0 0 0 0 1 0 0 0 0 1 5 2 0 0 0 0 0 0
Cytoplasm 360 487 16 – 19 109 135 161 77 71 95 60 203 173 275 150 168 154 334 467 39 159 222 192
Endoplasmic Reticulum 1 2 0 – 0 2 0 0 0 1 0 0 0 0 0 11 4 5 0 0 0 1 1 1
Endosome 0 1 0 – 0 0 2 0 0 0 2 0 2 0 0 0 0 1 2 1 0 3 0 2
Golgi 3 1 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 0 4 2 0 0 0 0
Mitochondria 5 4 0 – 1 5 10 14 41 30 60 46 2 0 1 2 2 1 3 5 0 3 1 5
Nucleus 2 2 0 – 0 5 3 3 2 3 4 6 0 1 0 1 1 3 1 0 0 2 0 0
Nuclear Periphery 0 2 0 – 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 1 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 1 4 2
Vac/Vac Membrane 1 2 0 – 0 0 15 19 3 3 8 14 2 0 2 3 12 13 1 2 0 3 2 3
Unique Cell Count 367 496 17 19 114 155 186 105 91 147 110 207 174 276 154 180 162 360 488 42 183 241 222
Labelled Cell Count 375 502 17 20 122 166 198 125 108 172 128 209 175 278 169 192 179 360 488 42 183 241 222


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.1 4.4 5.1 – 4.2 5.1 5.7 6.0 4.9 5.0 5.3 5.4 6.1 6.3 5.5 7.1 8.8 9.2 6.8 6.9 6.7
Std Deviation (1e-4) 1.1 0.8 0.4 – 1.0 1.0 1.5 1.4 1.2 0.9 1.3 1.2 1.4 1.3 1.2 1.3 1.7 1.6 1.7 1.6 1.7
Intensity Change (Log2) – – – – -0.27 0.0 0.17 0.24 -0.07 -0.01 0.05 0.08 0.27 0.31 0.12 0.48 0.79 0.86 0.41 0.43 0.41


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.6582 2.68 2.4644 2.2549 2.1715 2.378 8.0813 8.5553 8.8724 7.7468 8.5418 8.5042 4.7921 5.6426 5.4116 5.8643 5.3287 5.3343
Actin 0.0002 0.0007 0.0026 0.0004 0.0022 0.0015 0.03 0.0015 0.0095 0.0072 0.0552 0.0078 0.0118 0.0041 0.0076 0.0513 0.0068 0.0008
Bud 0.0002 0.0031 0.0106 0.0006 0.0011 0.0035 0.0065 0.0012 0.0024 0.002 0.0002 0.0008 0.0005 0.0008 0.0006 0.0006 0.0023 0.0002
Bud Neck 0.0003 0.0003 0.0004 0.0003 0.0007 0.0025 0.0185 0.0005 0.0029 0.0009 0.0016 0.0013 0.0006 0.0007 0.0003 0.0007 0.0056 0.0009
Bud Periphery 0.0001 0.0005 0.0026 0.0004 0.001 0.0016 0.0092 0.0004 0.0028 0.002 0.0002 0.001 0.0007 0.0007 0.0005 0.0003 0.0034 0.0001
Bud Site 0.0005 0.0051 0.0023 0.0003 0.0006 0.0036 0.0094 0.0047 0.0061 0.002 0.0004 0.0005 0.0018 0.0034 0.0006 0.0009 0.0094 0.0001
Cell Periphery 0 0.0002 0.0001 0 0.0001 0.0002 0.0008 0.0001 0.0003 0.0002 0 0.0001 0.0002 0.0002 0.0001 0.0001 0.0002 0.0001
Cytoplasm 0.8075 0.8197 0.8297 0.8242 0.8293 0.8581 0.5464 0.8832 0.7255 0.7721 0.6859 0.8095 0.7872 0.8985 0.8421 0.7666 0.8098 0.8799
Cytoplasmic Foci 0.0115 0.0073 0.0092 0.0108 0.0165 0.0112 0.046 0.0058 0.0223 0.0386 0.0289 0.0167 0.0269 0.0044 0.0082 0.0484 0.0376 0.014
Eisosomes 0 0 0 0 0 0 0.0005 0 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0 0.0002 0.0001 0
Endoplasmic Reticulum 0.0037 0.0029 0.0043 0.0041 0.0067 0.0031 0.0096 0.002 0.0027 0.0056 0.0014 0.0028 0.0047 0.0018 0.0029 0.0019 0.004 0.0026
Endosome 0.0058 0.0038 0.0043 0.0073 0.0091 0.0051 0.0378 0.0024 0.0186 0.0174 0.0041 0.007 0.0101 0.0012 0.0046 0.0091 0.0203 0.003
Golgi 0.0004 0.0002 0.0003 0.0003 0.0004 0.0016 0.0109 0.0003 0.0087 0.0163 0.0009 0.0023 0.0016 0.0004 0.0013 0.0058 0.0073 0.0003
Lipid Particles 0.0006 0.0001 0.0002 0.0001 0.0006 0.0013 0.0071 0.0001 0.0095 0.0072 0.0004 0.0016 0.0015 0.0003 0.0032 0.0046 0.0165 0.0001
Mitochondria 0.0004 0.0003 0.0011 0.0005 0.0007 0.0011 0.027 0.0003 0.0073 0.0095 0.0002 0.0022 0.0011 0.0003 0.0005 0.0011 0.0035 0.0008
None 0.1486 0.1354 0.1093 0.1335 0.1118 0.0869 0.1841 0.088 0.1599 0.0941 0.1898 0.122 0.1194 0.0761 0.1111 0.0436 0.0311 0.0856
Nuclear Periphery 0.002 0.0038 0.0035 0.0028 0.0024 0.0017 0.0227 0.0012 0.0015 0.0025 0.0007 0.004 0.011 0.0009 0.0024 0.0025 0.0071 0.001
Nucleolus 0.0003 0.0001 0.0002 0.0001 0.0002 0.0002 0.0009 0.0001 0.0004 0.0007 0.0002 0.0001 0.0004 0.0001 0.0001 0.0004 0.0016 0
Nucleus 0.0063 0.0128 0.0119 0.0088 0.0105 0.0109 0.0082 0.0062 0.0041 0.0077 0.0027 0.0054 0.0084 0.0038 0.0047 0.0051 0.0078 0.0072
Peroxisomes 0.0008 0.0003 0.0003 0.0001 0.0003 0.0008 0.0121 0.0003 0.0101 0.006 0.0009 0.0104 0.0032 0.0005 0.0022 0.0075 0.0072 0.0003
Punctate Nuclear 0.0089 0.0012 0.0054 0.0012 0.0026 0.0037 0.0049 0.0009 0.0024 0.0041 0.0257 0.0032 0.0061 0.0009 0.006 0.0484 0.0148 0.002
Vacuole 0.0017 0.0018 0.0014 0.0036 0.0027 0.0013 0.0051 0.0008 0.0024 0.0032 0.0004 0.0009 0.0021 0.0007 0.0008 0.0006 0.0026 0.0006
Vacuole Periphery 0.0002 0.0003 0.0003 0.0007 0.0004 0.0003 0.0023 0.0001 0.0005 0.0008 0.0001 0.0004 0.0008 0.0001 0.0002 0.0003 0.001 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 31.0107 21.9195 25.833 29.2858 32.7264 28.8312 38.7848 22.4375 33.5291 32.667
Translational Efficiency 1.3308 2.176 1.8489 1.3998 1.4816 1.2339 0.9173 1.8618 1.0729 0.9728

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Putative cytoplasmic short-chain dehydrogenase/reductase
Localization
Cell Percentages cytoplasm (45%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Nre1

Nre1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nre1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available