Standard name
Human Ortholog
Description ER associated glutathione S-transferase; capable of homodimerization; glutathione transferase for Yvc1p vacuolar cation channel; expression induced during the diauxic shift and throughout stationary phase; functional overlap with Gtt2p, Grx1p, and Grx2p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0 0.07 0 0.07 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0.06 0 0 0 0.1 0.09 0.12 0 0 0 0 0 0
Cytoplasm 0.94 0.94 0.97 0.96 0.76 0.74 0.63 – 0.67 0.64 0.64 0.63 0.98 0.98 0.95 0.72 0.81 0.73 0.55 0.57 0.5 0.68 0.61 0.51
Endoplasmic Reticulum 0 0 0 0 0.17 0.1 0.23 – 0.08 0.14 0.08 0.12 0 0 0.05 0.05 0 0.05 0.07 0.05 0.06 0 0.06 0.08
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.16 0 – 0.12 0.07 0.1 0.08 0 0 0 0 0 0 0.23 0.16 0.27 0.17 0.13 0.14
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.06 0 0.11 – 0.15 0.17 0.18 0.15 0 0 0 0.07 0.1 0.05 0 0.06 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 1 0 12 6 11 – 13 13 11 28 1 1 0 12 5 6 4 17 11 4 8 11
Bud 0 1 0 1 1 10 6 – 19 20 18 13 1 1 0 3 0 2 2 5 1 0 13 13
Bud Neck 1 0 11 4 3 2 0 – 0 0 0 1 0 0 0 0 0 0 2 7 4 1 2 5
Bud Site 0 0 0 0 0 1 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 4 10 6 15 6 14 – 12 18 17 33 4 2 8 17 11 10 2 4 6 1 6 1
Cytoplasm 225 340 287 262 288 214 294 – 295 294 280 352 234 309 403 118 100 60 150 223 149 110 185 146
Endoplasmic Reticulum 0 9 4 0 64 30 106 – 35 65 36 69 1 7 21 8 3 4 18 20 18 5 18 24
Endosome 5 2 1 0 5 1 4 – 3 7 5 5 0 1 3 7 7 2 7 15 2 2 3 10
Golgi 1 1 1 1 0 0 0 – 0 0 0 0 1 0 0 2 1 1 2 3 0 0 1 4
Mitochondria 9 3 1 7 2 47 16 – 55 32 46 44 0 1 1 2 3 3 63 62 80 27 38 39
Nucleus 0 2 1 0 4 4 3 – 4 4 5 0 0 0 1 0 0 0 0 0 0 0 0 1
Nuclear Periphery 0 0 0 0 0 0 0 – 2 0 0 3 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 1
SpindlePole 0 1 0 0 3 1 3 – 0 3 2 0 0 0 2 5 0 0 1 2 4 0 5 6
Vac/Vac Membrane 5 5 0 3 21 10 54 – 67 79 81 86 0 0 6 11 12 4 9 24 9 2 13 13
Unique Cell Count 240 360 295 272 380 291 470 441 457 439 560 239 316 426 164 124 82 272 394 301 162 302 285
Labelled Cell Count 247 369 317 284 418 332 511 506 535 501 634 243 322 445 185 142 92 272 394 301 162 302 285


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 4.5 4.9 5.1 7.5 5.8 7.9 – 7.5 8.6 9.1 9.4 5.1 6.0 6.7 8.5 9.4 8.6 4.7 4.8 5.5
Std Deviation (1e-4) 0.8 1.1 1.1 1.7 1.8 1.8 1.6 – 2.4 2.9 3.4 3.7 1.2 2.1 2.7 2.0 2.9 2.4 5.1 2.5 2.7
Intensity Change (Log2) – – – 0.06 0.61 0.22 0.68 – 0.61 0.81 0.88 0.92 0.06 0.29 0.44 0.79 0.93 0.81 -0.06 -0.03 0.15


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.6398 -0.7816 -0.8574 -1.1981 -0.8683 -0.9953 0.0038 0.5215 0.5325 1.1214 0.6808 0.4521 2.7702 2.896 2.923 2.4885 3.0004 3.0994
Actin 0.041 0.0006 0.0156 0.0055 0.0108 0.0039 0.0077 0.0065 0.008 0.058 0.0011 0.0121 0.002 0.001 0.0016 0.0047 0.0012 0.0008
Bud 0.0007 0.0005 0.0006 0.0058 0.0004 0.0002 0.0002 0.0079 0.0033 0.0068 0.0019 0.0015 0.0005 0.0007 0.001 0.0003 0.0009 0.0003
Bud Neck 0.0019 0.0002 0.001 0.0009 0.001 0.0011 0.0011 0.0003 0.005 0.0008 0.0004 0.0043 0.0054 0.0002 0.0003 0.0006 0.0003 0.0013
Bud Periphery 0.0011 0.002 0.0009 0.0252 0.0005 0.0005 0.0002 0.003 0.0021 0.0211 0.0017 0.0039 0.0006 0.0007 0.0011 0.0006 0.0035 0.0008
Bud Site 0.0048 0.0018 0.0018 0.0012 0.0008 0.0001 0.0046 0.005 0.0069 0.0062 0.0004 0.0006 0.0018 0.0074 0.0013 0.001 0.0006 0.0001
Cell Periphery 0.0006 0.0004 0.0006 0.0004 0.0004 0.0002 0.0007 0.0013 0.0006 0.0008 0.0002 0.0004 0.0009 0.0012 0.0008 0.0009 0.0004 0.0003
Cytoplasm 0.2385 0.3022 0.3514 0.3119 0.4992 0.4136 0.1924 0.3928 0.3152 0.2594 0.3719 0.3633 0.2637 0.334 0.2901 0.2231 0.4089 0.3255
Cytoplasmic Foci 0.0338 0.0418 0.0357 0.0216 0.0436 0.0247 0.0301 0.0146 0.0178 0.0533 0.0193 0.0141 0.0082 0.0235 0.0164 0.0101 0.0048 0.0086
Eisosomes 0.0005 0.0003 0.0004 0.0003 0.0002 0.0001 0.0004 0.0003 0.0003 0.0005 0.0002 0.0001 0.0003 0.0001 0.0002 0.0003 0.0002 0.0001
Endoplasmic Reticulum 0.0199 0.0118 0.0191 0.0067 0.0137 0.0109 0.0047 0.0085 0.0113 0.0064 0.0041 0.0079 0.0231 0.025 0.0361 0.0173 0.0097 0.0092
Endosome 0.0403 0.0136 0.025 0.0058 0.0313 0.0157 0.02 0.0038 0.0128 0.0643 0.0402 0.0055 0.0069 0.0158 0.0073 0.0056 0.0025 0.0022
Golgi 0.0134 0.0006 0.0065 0.0007 0.0035 0.0014 0.0052 0.0029 0.0078 0.0405 0.028 0.001 0.0008 0.001 0.0009 0.0007 0.0002 0.0002
Lipid Particles 0.0254 0.0035 0.0176 0.0012 0.0116 0.0039 0.0118 0.0014 0.0077 0.0064 0.0044 0.0012 0.0019 0.0043 0.0019 0.0008 0.0005 0.0005
Mitochondria 0.0167 0.0005 0.0077 0.0011 0.0272 0.0009 0.0042 0.001 0.0051 0.0735 0.0139 0.0012 0.0007 0.0007 0.0007 0.0005 0.0004 0.0003
None 0.5155 0.6089 0.4918 0.6041 0.3355 0.5139 0.6741 0.5401 0.5717 0.3742 0.4936 0.5601 0.6729 0.5762 0.631 0.7271 0.563 0.647
Nuclear Periphery 0.0111 0.0022 0.0065 0.0014 0.0036 0.0028 0.0033 0.0009 0.007 0.002 0.0011 0.0027 0.0024 0.0016 0.0013 0.0013 0.0007 0.0004
Nucleolus 0.0014 0.0004 0.0006 0.0002 0.0009 0.0002 0.0007 0.0002 0.0005 0.0007 0.0003 0.0004 0.0003 0.0002 0.0004 0.0003 0.0001 0.0001
Nucleus 0.0085 0.002 0.0023 0.0011 0.0025 0.0011 0.0141 0.0013 0.0048 0.0036 0.0014 0.0029 0.0021 0.0014 0.0016 0.0014 0.0006 0.0007
Peroxisomes 0.0145 0.0009 0.0063 0.0009 0.0042 0.0008 0.0183 0.0049 0.0064 0.0119 0.0013 0.0024 0.0004 0.0006 0.0008 0.0003 0.0002 0.0001
Punctate Nuclear 0.0048 0.002 0.0036 0.0014 0.0029 0.0023 0.0031 0.0014 0.0026 0.005 0.0019 0.0052 0.0008 0.001 0.003 0.0017 0.0003 0.0008
Vacuole 0.0042 0.0034 0.0043 0.0021 0.0048 0.0016 0.0025 0.0018 0.0024 0.0034 0.0096 0.0087 0.0038 0.0031 0.0022 0.0014 0.0008 0.0007
Vacuole Periphery 0.0014 0.0003 0.0011 0.0004 0.0015 0.0003 0.0004 0.0001 0.0006 0.0012 0.0031 0.0003 0.0003 0.0002 0.0003 0.0002 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 53.9964 25.1841 29.0697 28.2629 27.3947 46.9274 45.2686 38.1916 43.8585 45.5986
Translational Efficiency 1.3716 2.0116 1.4394 1.9959 1.4372 1.7251 1.4164 1.619 1.7387 1.656

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description ER associated glutathione S-transferase; capable of homodimerization; glutathione transferase for Yvc1p vacuolar cation channel; expression induced during the diauxic shift and throughout stationary phase; functional overlap with Gtt2p, Grx1p, and Grx2p
Localization
Cell Percentages cytoplasm (10%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Gtt1

Gtt1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gtt1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available