Standard name
Human Ortholog
Description Subunit of spindle-assembly checkpoint complex; involved in delaying anaphase onset in cells with defects in mitotic spindle assembly; pseudosubstrate inhibitor of APC(Cdc20), the anaphase promoting complex involved in securin (Pds1p) turnover; MAD3 has a paralog, BUB1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.56 0.63 0.67 0.62 0.35 0.25 0.24 0.25 0.27 0.22 0.19 0.23 0.72 0.79 0.8 0.35 0.25 0.24 0.44 0.39 0.39 0.65 0.72 0.75
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.07 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0.07 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.33 0 0.12 0.28 0.25 0.58 0.65 0.66 0.77 0.81 0.85 0.8 0.06 0 0 0.08 0 0 0.05 0.05 0.12 0 0 0
Nucleus 0.13 0.18 0.11 0.1 0.31 0.37 0.34 0.29 0.26 0.2 0.2 0.21 0.15 0.09 0.06 0.32 0.45 0.4 0.21 0.2 0.16 0.12 0.07 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.13 0.3 0.18 0.12 0.25 0.11 0.11 0.07 0.05 0.08 0 0.06 0.11 0.18 0.18 0.28 0.31 0.35 0.19 0.24 0.24 0.13 0.08 0.12
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 4 0 0 1 1
Bud 1 0 0 0 0 4 2 10 3 9 3 3 0 0 0 0 0 0 3 8 1 0 1 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 1 3 3
Bud Site 0 0 0 0 0 0 5 5 5 2 3 3 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 147 235 138 100 74 58 86 92 72 78 43 75 102 212 213 158 93 68 145 173 97 45 141 134
Endoplasmic Reticulum 1 2 1 0 0 1 0 0 1 0 0 1 1 0 0 29 27 20 0 1 3 0 2 0
Endosome 9 1 1 1 7 1 1 0 0 0 0 0 0 7 3 33 19 20 5 8 0 0 2 0
Golgi 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 2 2 1 1 0 0 0 0 0
Mitochondria 87 11 24 44 54 136 232 246 209 295 191 258 8 3 2 35 4 6 16 20 30 2 8 4
Nucleus 35 66 22 16 65 87 121 108 70 73 45 68 21 23 16 144 167 111 69 90 41 8 14 2
Nuclear Periphery 3 0 0 0 0 0 2 2 2 2 4 4 1 0 1 0 0 0 0 1 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 1 0 0 1 0 0 2 1 1 2 1 0 0 0 2 1 1 11 14 4 1 3 2
Vac/Vac Membrane 35 111 37 20 53 25 40 26 13 29 9 18 15 47 47 128 114 98 61 108 61 8 16 21
Unique Cell Count 263 374 207 160 213 236 358 373 270 362 224 321 141 268 266 450 370 278 330 445 252 70 198 179
Labelled Cell Count 319 427 223 181 254 312 491 493 376 489 300 432 148 292 282 531 427 325 330 445 252 70 198 179


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.4 4.2 3.8 3.9 4.0 3.5 3.4 3.4 3.2 2.9 2.9 2.9 4.6 4.4 4.3 5.9 6.7 6.1 4.0 4.4 4.9
Std Deviation (1e-4) 0.6 0.7 0.8 1.5 1.4 1.0 1.7 1.0 1.4 1.0 0.6 1.8 1.0 1.2 1.3 1.6 1.6 1.4 0.8 1.7 1.0
Intensity Change (Log2) – – – 0.01 0.07 -0.13 -0.17 -0.17 -0.25 -0.39 -0.42 -0.38 0.25 0.21 0.18 0.61 0.8 0.68 0.05 0.2 0.34


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 2.9 3.4 4.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.0276 -0.576 -0.6796 -0.4106 -1.059 -0.5053 1.1094 1.7118 1.546 2.5466 1.7383 1.7901 2.8158 2.7764 2.7099 3.8443 3.5387 3.097
Actin 0.0039 0.0015 0.0027 0.0001 0.0044 0.0019 0.0058 0.0023 0.0023 0.0011 0.0152 0.0009 0.0134 0.0003 0.0093 0.0034 0.001 0.0033
Bud 0.0001 0.0001 0.0001 0 0.0001 0.0001 0.001 0.0004 0.0005 0.0004 0.0004 0.0002 0.001 0.0041 0.0031 0.0003 0.0002 0.0004
Bud Neck 0.0014 0.0003 0.0003 0.0001 0.0007 0.0032 0.0021 0.0005 0.0011 0.0056 0.0128 0.0015 0.0094 0.0004 0.0003 0.0015 0.0019 0.0072
Bud Periphery 0.0001 0.0001 0.0001 0 0.0001 0.0002 0.0021 0.0004 0.001 0.0005 0.001 0.0004 0.0023 0.0024 0.0032 0.0004 0.0004 0.0008
Bud Site 0.0033 0.0018 0.0007 0 0.0003 0.0003 0.0067 0.004 0.0007 0.0172 0.002 0.0004 0.0022 0.0027 0.0012 0.0006 0.0006 0.0002
Cell Periphery 0.0001 0.0002 0.0001 0 0.0001 0.0001 0.0002 0.0001 0.0001 0.0005 0.0003 0.0001 0.0003 0.0002 0.0001 0.0001 0.0001 0.0001
Cytoplasm 0.1608 0.0759 0.108 0.0403 0.1388 0.1192 0.0818 0.0998 0.0778 0.0635 0.0685 0.1021 0.0648 0.1621 0.083 0.0529 0.0947 0.1172
Cytoplasmic Foci 0.052 0.0135 0.0048 0.0025 0.0094 0.0153 0.0078 0.0074 0.0041 0.0067 0.0112 0.008 0.0079 0.0049 0.0073 0.0076 0.0029 0.0104
Eisosomes 0.0004 0.0001 0.0004 0.0001 0.0007 0.0003 0.0002 0.0001 0.0001 0.0002 0.0008 0.0001 0.0004 0.0001 0.0001 0.0002 0.0001 0.0001
Endoplasmic Reticulum 0.0025 0.0022 0.0019 0.0014 0.0034 0.0019 0.002 0.0014 0.0016 0.0007 0.0015 0.0009 0.0022 0.0014 0.0015 0.0019 0.0025 0.0015
Endosome 0.0046 0.0035 0.0018 0.0004 0.0041 0.0051 0.0041 0.0034 0.0016 0.0198 0.0051 0.0021 0.0043 0.0019 0.0038 0.012 0.0034 0.0035
Golgi 0.0021 0.001 0.0002 0 0.0004 0.0005 0.0012 0.0002 0.0002 0.0031 0.0015 0.0002 0.0015 0.0001 0.0011 0.0016 0.0002 0.0008
Lipid Particles 0.006 0.0074 0.0015 0.0002 0.0019 0.0041 0.0025 0.0004 0.0004 0.0122 0.0026 0.001 0.0052 0.0002 0.002 0.0042 0.0005 0.0023
Mitochondria 0.0003 0.0008 0.0069 0.0002 0.0007 0.0006 0.0011 0.0005 0.0007 0.0027 0.0015 0.0005 0.0038 0.0009 0.0013 0.0011 0.0013 0.0007
None 0.6487 0.8062 0.7161 0.8056 0.712 0.6915 0.7865 0.6805 0.7779 0.6867 0.7461 0.743 0.8183 0.7135 0.7855 0.7657 0.7139 0.7013
Nuclear Periphery 0.0028 0.0036 0.0035 0.0018 0.0085 0.0053 0.0062 0.0051 0.0041 0.0029 0.0035 0.0032 0.0069 0.0034 0.0039 0.0111 0.0065 0.0036
Nucleolus 0.0013 0.0027 0.0015 0.0007 0.0085 0.0032 0.0017 0.0029 0.0021 0.0109 0.0017 0.002 0.002 0.0018 0.0016 0.0027 0.0092 0.0015
Nucleus 0.0903 0.0611 0.1311 0.1433 0.0833 0.1286 0.0653 0.1535 0.0973 0.1233 0.1054 0.0988 0.0188 0.0814 0.0737 0.0958 0.1315 0.1182
Peroxisomes 0.0062 0.0018 0.0048 0.0001 0.0008 0.0009 0.0049 0.0005 0.001 0.0036 0.0018 0.0006 0.0224 0.0003 0.0035 0.0014 0.0003 0.0031
Punctate Nuclear 0.0108 0.0143 0.0123 0.0027 0.0199 0.0156 0.015 0.0349 0.0247 0.0286 0.016 0.0335 0.011 0.0162 0.0135 0.0331 0.0267 0.0227
Vacuole 0.0022 0.0015 0.0012 0.0004 0.0017 0.0016 0.0014 0.0015 0.0007 0.009 0.0007 0.0005 0.0015 0.0015 0.0007 0.0017 0.0016 0.0009
Vacuole Periphery 0.0001 0.0002 0.0002 0.0001 0.0003 0.0004 0.0003 0.0002 0.0002 0.0009 0.0003 0.0002 0.0005 0.0002 0.0003 0.0008 0.0005 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.5535 8.9207 3.7826 6.1477 4.6208 7.124 11.7579 12.8277 10.4411 9.9666
Translational Efficiency 0.796 0.7379 1.0945 0.7696 1.161 1.1828 0.7409 0.6348 0.78 0.8746

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of spindle-assembly checkpoint complex; involved in delaying anaphase onset in cells with defects in mitotic spindle assembly; pseudosubstrate inhibitor of APC(Cdc20), the anaphase promoting complex involved in securin (Pds1p) turnover; MAD3 has a paralog, BUB1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (13%), nucleus (11%), mixed (55%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Mad3

Mad3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mad3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available