Standard name
Human Ortholog
Description Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex; role in anaphase progression; required for recovery after DSB repair; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.67 0.8 0.89 0.82 0.98 0.94 0.88 0.94 0.8 0.74 0.65 0.72 0.78 0.87 0.91 0.78 0.65 0.69 0.65 0.67 0.68 0.65 0.69 0.66
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.05 0 0 0 0.06 0.17 0.07 0.37 0.37 0.54 0.43 0 0 0 0 0 0 0 0 0.05 0 0 0
Nucleus 0.09 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0.05 0.18 0.09 0.07 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.33 0.17 0.13 0.13 0.05 0 0 0 0 0 0 0 0.25 0.17 0.12 0.25 0.37 0.35 0.07 0.11 0.14 0.17 0.16 0.16
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 3 0 2 4 7
Bud 1 2 0 1 0 2 5 0 2 10 6 4 2 1 2 0 0 0 0 1 0 6 9 21
Bud Neck 0 1 1 0 0 0 0 9 1 0 1 2 0 0 0 0 0 0 1 2 1 2 6 15
Bud Site 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 1 1 0 1 1 1 1 0 1 0 0 0 0 0 1 0 0 1 0
Cytoplasm 51 326 113 65 103 234 246 289 239 181 147 124 352 419 475 142 132 134 75 343 127 164 364 384
Endoplasmic Reticulum 2 7 1 1 0 0 0 1 0 0 0 0 2 1 1 5 12 14 0 1 0 3 7 6
Endosome 1 5 0 0 1 0 0 0 0 0 0 1 7 14 12 2 10 10 1 7 0 1 3 4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 2 1 0 0 2 0 0 1 1
Mitochondria 1 19 0 2 0 15 47 20 111 92 123 74 6 1 2 4 4 4 1 17 9 10 15 9
Nucleus 7 22 5 3 0 4 3 4 6 7 6 5 4 2 1 12 10 10 20 48 13 8 16 17
Nuclear Periphery 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 2
Nucleolus 0 1 0 0 0 0 0 0 0 0 0 0 1 0 1 0 1 0 0 1 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 0 0 0 0 1 1 1 0 1 2 0 0 0 0 0 0 1 12 3 4 5 6
Vac/Vac Membrane 25 68 16 10 5 5 5 8 4 10 7 5 113 83 65 46 75 67 8 58 26 43 83 92
Unique Cell Count 76 410 127 79 105 249 278 306 299 246 227 173 451 479 524 181 204 193 115 516 187 253 529 586
Labelled Cell Count 88 453 136 82 109 261 308 332 367 302 292 218 489 522 560 214 246 239 115 516 187 253 529 586


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 4.4 5.4 5.0 5.8 4.3 4.4 4.7 3.6 3.6 3.7 4.2 5.4 5.4 5.4 7.1 7.0 6.6 5.0 5.2 5.3
Std Deviation (1e-4) 0.9 0.5 0.9 1.5 2.0 1.5 1.3 1.5 0.8 0.8 1.0 1.2 1.1 1.0 1.1 1.1 1.4 1.4 1.2 2.2 1.5
Intensity Change (Log2) -0.1 0.1 -0.31 -0.28 -0.2 -0.57 -0.58 -0.55 -0.37 -0.01 0.01 0.01 0.4 0.38 0.29 -0.11 -0.06 -0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 2.8 4.8 4.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.5245 2.6096 2.54 2.2704 2.6191 2.3215 1.3959 1.2141 1.2637 0.8816 1.6407 1.2932 0.2873 0.6229 0.3925 0.7181 0.1985 0.4603
Actin 0.0347 0.0003 0.0096 0.0025 0.0343 0.0034 0.0068 0.0008 0.0068 0.0302 0.0358 0.0144 0.0136 0.0006 0.0011 0.0002 0.0029 0.0007
Bud 0.0014 0.002 0.0012 0.0004 0.001 0.0013 0.0037 0.0044 0.0022 0.0008 0.0032 0.0024 0.0003 0.0002 0.0006 0.0002 0.0106 0.0002
Bud Neck 0.008 0.0005 0.0034 0.0074 0.003 0.0028 0.0021 0.0008 0.0033 0.0031 0.0021 0.0066 0.002 0.0003 0.0008 0.0009 0.001 0.0027
Bud Periphery 0.0031 0.0015 0.0023 0.0003 0.0021 0.0053 0.0023 0.003 0.0046 0.0007 0.0078 0.0044 0.0003 0.0001 0.0004 0.0001 0.0064 0.0001
Bud Site 0.0059 0.0052 0.017 0.0041 0.0052 0.0007 0.0053 0.0057 0.0025 0.0032 0.0063 0.0022 0.0037 0.0013 0.0011 0.0003 0.0025 0.0002
Cell Periphery 0.001 0.0001 0.0007 0.0001 0.0007 0.0002 0.0004 0.0002 0.0002 0.0001 0.0008 0.0001 0.0002 0.0001 0.0001 0 0.0001 0
Cytoplasm 0.3355 0.5026 0.3578 0.3499 0.3717 0.4522 0.5442 0.6171 0.4967 0.5467 0.5508 0.5695 0.4929 0.6142 0.4896 0.4739 0.5006 0.5178
Cytoplasmic Foci 0.0308 0.0048 0.0186 0.019 0.0433 0.0242 0.0231 0.0121 0.0118 0.043 0.0357 0.0159 0.0172 0.0062 0.0063 0.0047 0.0122 0.0165
Eisosomes 0.0007 0.0001 0.0003 0.0001 0.0004 0.0001 0.0001 0 0.0001 0.0005 0.0003 0.0001 0.0002 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0088 0.0029 0.0049 0.0038 0.0063 0.0047 0.0173 0.0044 0.0104 0.0041 0.0059 0.0048 0.0071 0.004 0.0046 0.0037 0.0028 0.003
Endosome 0.0254 0.0019 0.0177 0.0079 0.0393 0.0093 0.0371 0.0084 0.0127 0.0181 0.0422 0.0118 0.0122 0.0042 0.0049 0.008 0.0125 0.0066
Golgi 0.0081 0.0001 0.0097 0.0055 0.0161 0.001 0.0043 0.0005 0.004 0.0044 0.0169 0.0032 0.002 0.0002 0.0002 0.0001 0.0021 0.0006
Lipid Particles 0.0161 0.0001 0.0072 0.0163 0.0141 0.001 0.0143 0.0002 0.0081 0.0031 0.0108 0.0031 0.0022 0.0002 0.0002 0.0001 0.0057 0.0012
Mitochondria 0.0139 0.0005 0.0267 0.0039 0.038 0.009 0.0072 0.0009 0.0124 0.0016 0.0472 0.0038 0.0012 0.0006 0.0008 0.001 0.0017 0.0007
None 0.3514 0.3727 0.3659 0.3819 0.2611 0.3568 0.179 0.2303 0.2305 0.142 0.1529 0.1836 0.2937 0.2738 0.3049 0.2742 0.3313 0.2086
Nuclear Periphery 0.0158 0.0033 0.0121 0.0049 0.006 0.0064 0.0374 0.0072 0.0109 0.0084 0.0076 0.0174 0.015 0.0055 0.0094 0.0135 0.0109 0.009
Nucleolus 0.0041 0.0005 0.0026 0.0009 0.0027 0.0011 0.0013 0.0007 0.006 0.0011 0.0008 0.0011 0.0024 0.0006 0.001 0.0014 0.0013 0.001
Nucleus 0.0701 0.094 0.0974 0.1597 0.0875 0.0848 0.0878 0.0898 0.147 0.1338 0.0478 0.1334 0.1018 0.0777 0.1597 0.2028 0.0709 0.2007
Peroxisomes 0.0257 0.0001 0.0125 0.0125 0.043 0.0034 0.0025 0.0003 0.0043 0.0149 0.0064 0.0027 0.006 0.0002 0.0003 0.0001 0.0009 0.0007
Punctate Nuclear 0.0329 0.0056 0.0288 0.0175 0.016 0.0296 0.0126 0.0103 0.021 0.0365 0.0082 0.0158 0.0221 0.0078 0.0118 0.0103 0.0211 0.0278
Vacuole 0.005 0.001 0.0025 0.0011 0.0071 0.002 0.0069 0.0027 0.0038 0.003 0.0062 0.0023 0.0031 0.002 0.002 0.0037 0.0015 0.0014
Vacuole Periphery 0.0016 0.0001 0.0012 0.0003 0.001 0.0005 0.0043 0.0004 0.0008 0.0006 0.0042 0.0013 0.0007 0.0003 0.0004 0.0008 0.0008 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.0349 5.0703 5.4989 11.0342 7.071 7.8491 9.3659 9.9811 8.8918 12.6186
Translational Efficiency 0.4883 0.8838 0.9357 0.5055 0.6688 0.6521 0.6292 0.4583 0.7024 0.4962

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex; role in anaphase progression; required for recovery after DSB repair; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p
Localization
Cell Percentages cytoplasm (28%), nucleus (12%), mixed (47%)
Cell Cycle Regulation Yes
nucleus - S/G2 (1.4e-06)
Subcompartmental Group N/A

Rtt101

Rtt101


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rtt101-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available