Standard name | |
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Human Ortholog | |
Description | Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex; role in anaphase progression; required for recovery after DSB repair; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 3 | 0 | 2 | 4 | 7 |
Bud | 1 | 2 | 0 | 1 | 0 | 2 | 5 | 0 | 2 | 10 | 6 | 4 | 2 | 1 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 6 | 9 | 21 |
Bud Neck | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 9 | 1 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 2 | 6 | 15 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 |
Cytoplasm | 51 | 326 | 113 | 65 | 103 | 234 | 246 | 289 | 239 | 181 | 147 | 124 | 352 | 419 | 475 | 142 | 132 | 134 | 75 | 343 | 127 | 164 | 364 | 384 |
Endoplasmic Reticulum | 2 | 7 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 5 | 12 | 14 | 0 | 1 | 0 | 3 | 7 | 6 |
Endosome | 1 | 5 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 7 | 14 | 12 | 2 | 10 | 10 | 1 | 7 | 0 | 1 | 3 | 4 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 2 | 1 | 0 | 0 | 2 | 0 | 0 | 1 | 1 |
Mitochondria | 1 | 19 | 0 | 2 | 0 | 15 | 47 | 20 | 111 | 92 | 123 | 74 | 6 | 1 | 2 | 4 | 4 | 4 | 1 | 17 | 9 | 10 | 15 | 9 |
Nucleus | 7 | 22 | 5 | 3 | 0 | 4 | 3 | 4 | 6 | 7 | 6 | 5 | 4 | 2 | 1 | 12 | 10 | 10 | 20 | 48 | 13 | 8 | 16 | 17 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Nucleolus | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 12 | 3 | 4 | 5 | 6 |
Vac/Vac Membrane | 25 | 68 | 16 | 10 | 5 | 5 | 5 | 8 | 4 | 10 | 7 | 5 | 113 | 83 | 65 | 46 | 75 | 67 | 8 | 58 | 26 | 43 | 83 | 92 |
Unique Cell Count | 76 | 410 | 127 | 79 | 105 | 249 | 278 | 306 | 299 | 246 | 227 | 173 | 451 | 479 | 524 | 181 | 204 | 193 | 115 | 516 | 187 | 253 | 529 | 586 |
Labelled Cell Count | 88 | 453 | 136 | 82 | 109 | 261 | 308 | 332 | 367 | 302 | 292 | 218 | 489 | 522 | 560 | 214 | 246 | 239 | 115 | 516 | 187 | 253 | 529 | 586 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.2 | 4.4 | 5.4 | 5.0 | 5.8 | 4.3 | 4.4 | 4.7 | 3.6 | 3.6 | 3.7 | 4.2 | 5.4 | 5.4 | 5.4 | 7.1 | 7.0 | 6.6 | 5.0 | 5.2 | 5.3 |
Std Deviation (1e-4) | 0.9 | 0.5 | 0.9 | 1.5 | 2.0 | 1.5 | 1.3 | 1.5 | 0.8 | 0.8 | 1.0 | 1.2 | 1.1 | 1.0 | 1.1 | 1.1 | 1.4 | 1.4 | 1.2 | 2.2 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.1 | 0.1 | -0.31 | -0.28 | -0.2 | -0.57 | -0.58 | -0.55 | -0.37 | -0.01 | 0.01 | 0.01 | 0.4 | 0.38 | 0.29 | -0.11 | -0.06 | -0.03 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 2.8 | 4.8 | 4.4 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.5245 | 2.6096 | 2.54 | 2.2704 | 2.6191 | 2.3215 | 1.3959 | 1.2141 | 1.2637 | 0.8816 | 1.6407 | 1.2932 | 0.2873 | 0.6229 | 0.3925 | 0.7181 | 0.1985 | 0.4603 |
Actin | 0.0347 | 0.0003 | 0.0096 | 0.0025 | 0.0343 | 0.0034 | 0.0068 | 0.0008 | 0.0068 | 0.0302 | 0.0358 | 0.0144 | 0.0136 | 0.0006 | 0.0011 | 0.0002 | 0.0029 | 0.0007 |
Bud | 0.0014 | 0.002 | 0.0012 | 0.0004 | 0.001 | 0.0013 | 0.0037 | 0.0044 | 0.0022 | 0.0008 | 0.0032 | 0.0024 | 0.0003 | 0.0002 | 0.0006 | 0.0002 | 0.0106 | 0.0002 |
Bud Neck | 0.008 | 0.0005 | 0.0034 | 0.0074 | 0.003 | 0.0028 | 0.0021 | 0.0008 | 0.0033 | 0.0031 | 0.0021 | 0.0066 | 0.002 | 0.0003 | 0.0008 | 0.0009 | 0.001 | 0.0027 |
Bud Periphery | 0.0031 | 0.0015 | 0.0023 | 0.0003 | 0.0021 | 0.0053 | 0.0023 | 0.003 | 0.0046 | 0.0007 | 0.0078 | 0.0044 | 0.0003 | 0.0001 | 0.0004 | 0.0001 | 0.0064 | 0.0001 |
Bud Site | 0.0059 | 0.0052 | 0.017 | 0.0041 | 0.0052 | 0.0007 | 0.0053 | 0.0057 | 0.0025 | 0.0032 | 0.0063 | 0.0022 | 0.0037 | 0.0013 | 0.0011 | 0.0003 | 0.0025 | 0.0002 |
Cell Periphery | 0.001 | 0.0001 | 0.0007 | 0.0001 | 0.0007 | 0.0002 | 0.0004 | 0.0002 | 0.0002 | 0.0001 | 0.0008 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 |
Cytoplasm | 0.3355 | 0.5026 | 0.3578 | 0.3499 | 0.3717 | 0.4522 | 0.5442 | 0.6171 | 0.4967 | 0.5467 | 0.5508 | 0.5695 | 0.4929 | 0.6142 | 0.4896 | 0.4739 | 0.5006 | 0.5178 |
Cytoplasmic Foci | 0.0308 | 0.0048 | 0.0186 | 0.019 | 0.0433 | 0.0242 | 0.0231 | 0.0121 | 0.0118 | 0.043 | 0.0357 | 0.0159 | 0.0172 | 0.0062 | 0.0063 | 0.0047 | 0.0122 | 0.0165 |
Eisosomes | 0.0007 | 0.0001 | 0.0003 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0005 | 0.0003 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0088 | 0.0029 | 0.0049 | 0.0038 | 0.0063 | 0.0047 | 0.0173 | 0.0044 | 0.0104 | 0.0041 | 0.0059 | 0.0048 | 0.0071 | 0.004 | 0.0046 | 0.0037 | 0.0028 | 0.003 |
Endosome | 0.0254 | 0.0019 | 0.0177 | 0.0079 | 0.0393 | 0.0093 | 0.0371 | 0.0084 | 0.0127 | 0.0181 | 0.0422 | 0.0118 | 0.0122 | 0.0042 | 0.0049 | 0.008 | 0.0125 | 0.0066 |
Golgi | 0.0081 | 0.0001 | 0.0097 | 0.0055 | 0.0161 | 0.001 | 0.0043 | 0.0005 | 0.004 | 0.0044 | 0.0169 | 0.0032 | 0.002 | 0.0002 | 0.0002 | 0.0001 | 0.0021 | 0.0006 |
Lipid Particles | 0.0161 | 0.0001 | 0.0072 | 0.0163 | 0.0141 | 0.001 | 0.0143 | 0.0002 | 0.0081 | 0.0031 | 0.0108 | 0.0031 | 0.0022 | 0.0002 | 0.0002 | 0.0001 | 0.0057 | 0.0012 |
Mitochondria | 0.0139 | 0.0005 | 0.0267 | 0.0039 | 0.038 | 0.009 | 0.0072 | 0.0009 | 0.0124 | 0.0016 | 0.0472 | 0.0038 | 0.0012 | 0.0006 | 0.0008 | 0.001 | 0.0017 | 0.0007 |
None | 0.3514 | 0.3727 | 0.3659 | 0.3819 | 0.2611 | 0.3568 | 0.179 | 0.2303 | 0.2305 | 0.142 | 0.1529 | 0.1836 | 0.2937 | 0.2738 | 0.3049 | 0.2742 | 0.3313 | 0.2086 |
Nuclear Periphery | 0.0158 | 0.0033 | 0.0121 | 0.0049 | 0.006 | 0.0064 | 0.0374 | 0.0072 | 0.0109 | 0.0084 | 0.0076 | 0.0174 | 0.015 | 0.0055 | 0.0094 | 0.0135 | 0.0109 | 0.009 |
Nucleolus | 0.0041 | 0.0005 | 0.0026 | 0.0009 | 0.0027 | 0.0011 | 0.0013 | 0.0007 | 0.006 | 0.0011 | 0.0008 | 0.0011 | 0.0024 | 0.0006 | 0.001 | 0.0014 | 0.0013 | 0.001 |
Nucleus | 0.0701 | 0.094 | 0.0974 | 0.1597 | 0.0875 | 0.0848 | 0.0878 | 0.0898 | 0.147 | 0.1338 | 0.0478 | 0.1334 | 0.1018 | 0.0777 | 0.1597 | 0.2028 | 0.0709 | 0.2007 |
Peroxisomes | 0.0257 | 0.0001 | 0.0125 | 0.0125 | 0.043 | 0.0034 | 0.0025 | 0.0003 | 0.0043 | 0.0149 | 0.0064 | 0.0027 | 0.006 | 0.0002 | 0.0003 | 0.0001 | 0.0009 | 0.0007 |
Punctate Nuclear | 0.0329 | 0.0056 | 0.0288 | 0.0175 | 0.016 | 0.0296 | 0.0126 | 0.0103 | 0.021 | 0.0365 | 0.0082 | 0.0158 | 0.0221 | 0.0078 | 0.0118 | 0.0103 | 0.0211 | 0.0278 |
Vacuole | 0.005 | 0.001 | 0.0025 | 0.0011 | 0.0071 | 0.002 | 0.0069 | 0.0027 | 0.0038 | 0.003 | 0.0062 | 0.0023 | 0.0031 | 0.002 | 0.002 | 0.0037 | 0.0015 | 0.0014 |
Vacuole Periphery | 0.0016 | 0.0001 | 0.0012 | 0.0003 | 0.001 | 0.0005 | 0.0043 | 0.0004 | 0.0008 | 0.0006 | 0.0042 | 0.0013 | 0.0007 | 0.0003 | 0.0004 | 0.0008 | 0.0008 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 10.0349 | 5.0703 | 5.4989 | 11.0342 | 7.071 | 7.8491 | 9.3659 | 9.9811 | 8.8918 | 12.6186 |
Translational Efficiency | 0.4883 | 0.8838 | 0.9357 | 0.5055 | 0.6688 | 0.6521 | 0.6292 | 0.4583 | 0.7024 | 0.4962 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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