Standard name
Human Ortholog
Description Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.51 0.49 0.89 0.84 0.87 0.67 0.67 – 0.52 0.41 0.31 0.41 0.46 0.54 0.62 0.34 0.31 0.29 0.31 0.33 0.54 0.34 0.36 0.43
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.11 0.15 0 0.11 0.06 0.3 0.31 – 0.54 0.64 0.73 0.53 0.14 0.09 0.06 0 0 0.05 0 0.05 0.08 0.1 0.11 0.07
Nucleus 0.15 0.15 0 0.07 0.05 0.08 0.09 – 0.12 0.12 0.12 0.16 0.22 0.13 0.1 0.45 0.45 0.39 0.26 0.26 0.07 0.1 0.07 0.05
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.33 0.34 0.05 0 0.06 0.07 0 – 0.05 0.05 0 0 0.26 0.32 0.3 0.21 0.34 0.31 0.24 0.25 0.2 0.37 0.33 0.28
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 2 1 2 3 3 6
Bud 0 1 1 1 0 1 4 – 7 11 9 5 1 0 0 0 0 0 4 4 0 4 12 12
Bud Neck 0 0 11 1 8 2 10 – 1 8 3 4 0 0 0 0 0 0 1 1 0 3 3 7
Bud Site 0 0 0 0 0 0 2 – 4 2 8 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 0 0 0 2 – 0 1 0 1 0 0 0 0 0 0 0 0 1 0 0 1
Cytoplasm 95 137 220 132 278 202 305 – 184 123 88 131 127 205 266 46 34 33 76 111 155 98 152 195
Endoplasmic Reticulum 0 1 2 0 0 0 0 – 0 0 1 1 1 2 1 6 4 6 0 1 2 2 7 6
Endosome 4 2 0 1 0 0 0 – 0 0 0 0 1 10 5 6 2 5 6 4 0 0 4 6
Golgi 1 0 0 0 0 0 0 – 1 0 0 0 0 1 1 0 0 1 5 1 2 0 3 1
Mitochondria 21 42 5 17 19 91 142 – 193 193 208 167 39 33 26 6 1 6 10 18 23 28 46 33
Nucleus 28 42 10 11 15 23 42 – 43 36 34 51 61 50 42 61 49 45 65 88 19 30 28 21
Nuclear Periphery 0 0 0 0 0 0 0 – 0 2 3 0 0 1 1 0 0 1 1 0 0 0 0 0
Nucleolus 1 2 0 0 0 0 0 – 0 0 0 0 1 0 0 0 0 0 1 1 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 2
SpindlePole 0 1 1 1 4 2 7 – 2 3 0 3 0 2 1 1 0 0 9 11 7 8 8 16
Vac/Vac Membrane 61 94 13 5 18 20 14 – 17 15 8 14 72 121 128 28 37 36 59 87 57 106 137 125
Unique Cell Count 185 278 246 158 318 300 452 356 303 284 318 278 381 429 135 108 115 250 344 288 292 421 450
Labelled Cell Count 211 322 264 169 342 341 528 452 394 362 379 303 425 472 154 127 133 250 344 288 292 421 450


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.5 4.0 4.6 4.2 4.7 3.8 3.9 – 3.2 3.2 3.1 3.6 4.1 4.0 4.3 6.1 6.5 6.2 3.9 4.1 4.4
Std Deviation (1e-4) 1.3 0.8 1.1 1.2 1.5 1.4 1.4 – 1.0 1.3 1.1 0.8 1.7 1.0 1.4 1.5 1.9 1.8 1.7 1.7 1.9
Intensity Change (Log2) – – – -0.11 0.05 -0.28 -0.24 – -0.5 -0.51 -0.57 -0.36 -0.16 -0.2 -0.11 0.41 0.49 0.44 -0.22 -0.17 -0.07


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 4.7 7.2 6.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.5103 2.0136 1.9916 1.9004 1.357 2.148 0.3147 -0.0985 0.0576 -0.2129 0.2027 -0.1292 -0.2401 -0.1098 -0.0051 -0.0543 -0.659 -0.3476
Actin 0.0818 0.0002 0.0204 0.0443 0.0289 0.0028 0.038 0.0029 0.02 0.0024 0.005 0.0072 0.0021 0.0013 0.0016 0.0047 0.0006 0.0022
Bud 0.0009 0.0005 0.0003 0.0014 0.0001 0.0003 0.0061 0.0012 0.0011 0.0003 0.0004 0.0002 0.0061 0.0159 0.0047 0.0025 0.0154 0.0037
Bud Neck 0.0094 0.0005 0.0013 0.0049 0.0012 0.0048 0.0024 0.0005 0.0016 0.0019 0.0016 0.003 0.0011 0.0018 0.0012 0.0022 0.002 0.0078
Bud Periphery 0.0017 0.0004 0.0003 0.0014 0.0002 0.0009 0.0061 0.0005 0.0025 0.0006 0.0005 0.0002 0.0032 0.0045 0.0018 0.0007 0.0055 0.0018
Bud Site 0.0178 0.0103 0.0016 0.0187 0.0004 0.0006 0.0161 0.0039 0.0025 0.0008 0.001 0.0003 0.0089 0.0204 0.0051 0.0065 0.002 0.0006
Cell Periphery 0.0005 0.0001 0.0001 0.0004 0.0001 0.0003 0.0006 0.0001 0.0002 0.0002 0.0002 0.0001 0.0003 0.0004 0.0002 0.0001 0.0001 0.0001
Cytoplasm 0.0878 0.1437 0.1043 0.0835 0.1005 0.1378 0.1045 0.2217 0.1659 0.1574 0.2634 0.1408 0.097 0.0997 0.0768 0.0653 0.1074 0.0904
Cytoplasmic Foci 0.0276 0.0057 0.0086 0.0089 0.0046 0.0126 0.024 0.0092 0.0179 0.0217 0.027 0.0199 0.0779 0.0157 0.0296 0.0261 0.0247 0.0174
Eisosomes 0.0013 0.0002 0.0004 0.0004 0.0003 0.0003 0.0005 0.0001 0.0003 0.0001 0.0003 0.0001 0.0002 0.0001 0.0001 0.0001 0 0
Endoplasmic Reticulum 0.0062 0.0034 0.0037 0.0058 0.0022 0.0031 0.0074 0.0059 0.0063 0.0026 0.0032 0.0022 0.0037 0.0024 0.0017 0.0035 0.0011 0.0017
Endosome 0.0235 0.0029 0.0062 0.0096 0.0028 0.0127 0.0338 0.0074 0.0255 0.0446 0.0432 0.0082 0.0227 0.0017 0.0127 0.007 0.0145 0.0086
Golgi 0.0138 0.0001 0.0022 0.0084 0.0027 0.0052 0.0122 0.0005 0.0105 0.0112 0.0347 0.0095 0.0039 0.0003 0.0053 0.0027 0.0081 0.0006
Lipid Particles 0.0185 0.0013 0.0065 0.0047 0.0062 0.0099 0.023 0.0009 0.012 0.0203 0.0383 0.0121 0.0254 0.0007 0.0053 0.0148 0.0143 0.0005
Mitochondria 0.0156 0.0006 0.0019 0.0037 0.0009 0.0186 0.0293 0.0014 0.0099 0.0281 0.089 0.0074 0.0112 0.0015 0.0155 0.0165 0.0017 0.0008
None 0.3817 0.5612 0.5414 0.4364 0.4797 0.4165 0.2485 0.3193 0.2767 0.1842 0.2833 0.214 0.2402 0.148 0.236 0.0949 0.1991 0.0954
Nuclear Periphery 0.031 0.0096 0.0104 0.0215 0.006 0.0146 0.017 0.0213 0.0295 0.0149 0.0124 0.0089 0.0109 0.0123 0.0107 0.0231 0.0088 0.0114
Nucleolus 0.0077 0.0035 0.0048 0.003 0.0041 0.0058 0.0063 0.0038 0.0032 0.0042 0.0022 0.004 0.0084 0.0099 0.0084 0.0099 0.0111 0.0072
Nucleus 0.1986 0.2281 0.2429 0.3214 0.3453 0.3142 0.3758 0.368 0.3594 0.4661 0.1359 0.5141 0.2488 0.4095 0.3321 0.3866 0.3405 0.4398
Peroxisomes 0.0234 0.0002 0.0069 0.0022 0.0024 0.0034 0.0172 0.002 0.0112 0.0029 0.0213 0.0036 0.0055 0.0017 0.0053 0.0343 0.0014 0.0004
Punctate Nuclear 0.0458 0.0246 0.0342 0.0166 0.0103 0.0326 0.0235 0.0251 0.0383 0.0283 0.0307 0.0418 0.2143 0.2507 0.2442 0.2957 0.2394 0.3057
Vacuole 0.0031 0.0024 0.0016 0.0021 0.0007 0.0023 0.0057 0.0037 0.004 0.005 0.004 0.0017 0.0063 0.0012 0.0012 0.002 0.0019 0.0035
Vacuole Periphery 0.0023 0.0003 0.0004 0.0008 0.0002 0.0008 0.0022 0.0006 0.0014 0.0023 0.0024 0.0006 0.0021 0.0003 0.0004 0.0006 0.0004 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.6021 7.0909 7.4772 8.2328 7.8486 5.8415 8.7733 9.2959 9.306 12.7846
Translational Efficiency 0.3716 0.4742 0.4474 0.318 0.4389 0.6716 0.413 0.3666 0.4581 0.3582

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains
Localization
Cell Percentages nucleus (25%), cytoplasm (5%), mixed (52%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Zap1

Zap1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Zap1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available