Standard name | |
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Human Ortholog | |
Description | Protein kinase of unknown cellular role; putative serine/threonine kinase; expression is induced during mild heat stress; deletion mutants are hypersensitive to copper sulphate and resistant to sorbate; interacts with an N-terminal fragment of Sst2p |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 5 | 4 | 2 | 3 | 6 | 7 |
Bud | 3 | 0 | 0 | – | 0 | 2 | 1 | 2 | 3 | 2 | 4 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 11 | 10 |
Bud Neck | 0 | 2 | 1 | – | 0 | 3 | 2 | 4 | 0 | 0 | 2 | 2 | 3 | 1 | 0 | 5 | 2 | 0 | 1 | 3 | 1 | 0 | 1 | 3 |
Bud Site | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 5 | 2 | 1 | – | 1 | 5 | 2 | 1 | 0 | 1 | 0 | 4 | 2 | 1 | 5 | 2 | 1 | 3 | 2 | 3 | 1 | 0 | 3 | 5 |
Cytoplasm | 362 | 321 | 80 | – | 114 | 178 | 248 | 221 | 66 | 140 | 96 | 179 | 365 | 478 | 623 | 335 | 253 | 223 | 283 | 271 | 53 | 149 | 405 | 442 |
Endoplasmic Reticulum | 2 | 10 | 1 | – | 0 | 7 | 6 | 3 | 5 | 2 | 3 | 6 | 2 | 1 | 0 | 21 | 18 | 16 | 13 | 7 | 2 | 1 | 2 | 2 |
Endosome | 1 | 0 | 0 | – | 1 | 0 | 5 | 1 | 0 | 0 | 0 | 5 | 1 | 2 | 1 | 6 | 0 | 1 | 5 | 1 | 2 | 1 | 4 | 4 |
Golgi | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 1 |
Mitochondria | 17 | 3 | 0 | – | 0 | 9 | 25 | 24 | 36 | 39 | 91 | 60 | 0 | 0 | 0 | 13 | 3 | 2 | 61 | 35 | 23 | 7 | 23 | 19 |
Nucleus | 0 | 1 | 1 | – | 0 | 1 | 2 | 2 | 2 | 2 | 1 | 2 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 3 | 0 |
Nuclear Periphery | 1 | 2 | 0 | – | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 2 | 0 | 1 | – | 1 | 1 | 4 | 2 | 0 | 0 | 6 | 6 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 4 | 0 | 0 | 5 | 8 |
Vac/Vac Membrane | 5 | 4 | 0 | – | 1 | 1 | 5 | 7 | 1 | 10 | 3 | 7 | 0 | 0 | 3 | 11 | 6 | 3 | 17 | 6 | 6 | 2 | 1 | 8 |
Unique Cell Count | 380 | 334 | 83 | 116 | 197 | 283 | 251 | 98 | 177 | 174 | 249 | 370 | 485 | 629 | 358 | 262 | 229 | 406 | 347 | 102 | 172 | 485 | 522 | |
Labelled Cell Count | 400 | 345 | 85 | 118 | 208 | 300 | 268 | 114 | 200 | 209 | 275 | 375 | 486 | 636 | 400 | 286 | 250 | 406 | 347 | 102 | 172 | 485 | 522 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.8 | 4.2 | 5.3 | – | 5.8 | 4.6 | 5.0 | 5.2 | 3.7 | 4.5 | 3.8 | 4.2 | 5.3 | 5.3 | 5.5 | 5.7 | 5.6 | 5.0 | 5.3 | 5.1 | 5.5 |
Std Deviation (1e-4) | 0.7 | 0.7 | 1.7 | – | 1.6 | 1.0 | 1.8 | 1.5 | 0.9 | 1.4 | 0.9 | 1.1 | 1.2 | 1.3 | 1.5 | 1.1 | 1.8 | 1.0 | 1.6 | 1.4 | 1.6 |
Intensity Change (Log2) | – | – | – | – | 0.13 | -0.21 | -0.09 | -0.04 | -0.5 | -0.22 | -0.47 | -0.33 | 0.01 | 0.01 | 0.06 | 0.1 | 0.08 | -0.07 | -0.01 | -0.04 | 0.05 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.27 | -0.2808 | -0.2057 | -0.1874 | 0.0728 | -0.3839 | -0.6591 | -0.4987 | -0.3714 | -0.7108 | -0.4833 | -0.5075 | -1.1643 | -0.7281 | -1.0387 | -0.8984 | -0.8518 | -0.7486 |
Actin | 0.01 | 0.0006 | 0.0246 | 0.0182 | 0.0523 | 0.0004 | 0.0312 | 0.0003 | 0.0028 | 0.0026 | 0.0039 | 0.0018 | 0.0331 | 0.0009 | 0.0097 | 0.0003 | 0.0159 | 0.0146 |
Bud | 0.002 | 0.0012 | 0.0034 | 0.0004 | 0.001 | 0.0008 | 0.0066 | 0.0138 | 0.0066 | 0.0046 | 0.0005 | 0.0005 | 0.0007 | 0.0067 | 0.0016 | 0.0001 | 0.0001 | 0.0003 |
Bud Neck | 0.011 | 0.0002 | 0.0044 | 0.0009 | 0.001 | 0.0009 | 0.0081 | 0.0001 | 0.0004 | 0.0023 | 0.0011 | 0.0002 | 0.0026 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0.0163 |
Bud Periphery | 0.0056 | 0.0038 | 0.0056 | 0.0009 | 0.0015 | 0.0013 | 0.0053 | 0.0019 | 0.0074 | 0.0019 | 0.0014 | 0.0004 | 0.0023 | 0.0056 | 0.0026 | 0.0004 | 0.0003 | 0.0005 |
Bud Site | 0.0074 | 0.0071 | 0.0158 | 0.0002 | 0.0091 | 0.0001 | 0.0164 | 0.0095 | 0.0125 | 0.0041 | 0.0007 | 0.0004 | 0.0015 | 0.0041 | 0.0024 | 0 | 0.0003 | 0.0008 |
Cell Periphery | 0.0014 | 0.0003 | 0.001 | 0.0001 | 0.0004 | 0.0001 | 0.0005 | 0.0003 | 0.0003 | 0.0005 | 0.0002 | 0.0001 | 0.0003 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Cytoplasm | 0.0329 | 0.0338 | 0.0392 | 0.0473 | 0.0304 | 0.0857 | 0.0196 | 0.0712 | 0.0278 | 0.0343 | 0.0671 | 0.0578 | 0.0832 | 0.1721 | 0.1608 | 0.2105 | 0.1748 | 0.2129 |
Cytoplasmic Foci | 0.0203 | 0.0073 | 0.0226 | 0.011 | 0.0287 | 0.0229 | 0.0178 | 0.0045 | 0.0219 | 0.0324 | 0.0038 | 0.0049 | 0.0183 | 0.0054 | 0.0163 | 0.0026 | 0.0134 | 0.0131 |
Eisosomes | 0.0006 | 0.0003 | 0.0005 | 0.0002 | 0.0008 | 0.0002 | 0.0023 | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0007 | 0.0003 | 0.0003 | 0.0001 | 0.0002 | 0.0001 |
Endoplasmic Reticulum | 0.0099 | 0.0017 | 0.0029 | 0.0018 | 0.0027 | 0.0009 | 0.0015 | 0.0017 | 0.001 | 0.0166 | 0.0016 | 0.0007 | 0.0056 | 0.003 | 0.0052 | 0.0018 | 0.0048 | 0.004 |
Endosome | 0.0179 | 0.0013 | 0.0117 | 0.0131 | 0.0585 | 0.0037 | 0.0179 | 0.002 | 0.0107 | 0.0131 | 0.0232 | 0.0024 | 0.0199 | 0.0019 | 0.0145 | 0.0007 | 0.0089 | 0.0074 |
Golgi | 0.0068 | 0.0003 | 0.0069 | 0.0199 | 0.0214 | 0.0005 | 0.0089 | 0.0004 | 0.0058 | 0.0081 | 0.0078 | 0.0038 | 0.0057 | 0.0002 | 0.002 | 0.0001 | 0.0055 | 0.0014 |
Lipid Particles | 0.0153 | 0.0008 | 0.0097 | 0.0032 | 0.0411 | 0.0013 | 0.0195 | 0.0007 | 0.0092 | 0.0051 | 0.0057 | 0.0013 | 0.007 | 0.0009 | 0.0073 | 0.0002 | 0.0148 | 0.0041 |
Mitochondria | 0.0166 | 0.0011 | 0.0114 | 0.0445 | 0.0447 | 0.0006 | 0.007 | 0.0003 | 0.0131 | 0.0035 | 0.0047 | 0.008 | 0.0017 | 0.0005 | 0.0067 | 0.0003 | 0.001 | 0.0052 |
None | 0.8187 | 0.9338 | 0.8121 | 0.8306 | 0.6598 | 0.8691 | 0.8074 | 0.8885 | 0.8646 | 0.8522 | 0.8699 | 0.9053 | 0.7912 | 0.7935 | 0.7521 | 0.7802 | 0.7432 | 0.7036 |
Nuclear Periphery | 0.0034 | 0.0005 | 0.0025 | 0.0011 | 0.0016 | 0.0015 | 0.0028 | 0.0004 | 0.0004 | 0.0076 | 0.0011 | 0.0008 | 0.0094 | 0.0008 | 0.0023 | 0.0007 | 0.0044 | 0.0054 |
Nucleolus | 0.0007 | 0.0005 | 0.0011 | 0.0002 | 0.0013 | 0.0003 | 0.0015 | 0.0004 | 0.0006 | 0.0008 | 0.0003 | 0.0037 | 0.0003 | 0.0003 | 0.0005 | 0.0001 | 0.0003 | 0.0008 |
Nucleus | 0.0015 | 0.0008 | 0.0014 | 0.0006 | 0.0011 | 0.0043 | 0.0037 | 0.0015 | 0.0019 | 0.0018 | 0.0005 | 0.0018 | 0.0019 | 0.0011 | 0.0017 | 0.0006 | 0.0013 | 0.0022 |
Peroxisomes | 0.0122 | 0.0013 | 0.0097 | 0.0026 | 0.026 | 0.0011 | 0.0129 | 0.0001 | 0.008 | 0.0033 | 0.0008 | 0.0004 | 0.0088 | 0.0002 | 0.0061 | 0.0001 | 0.0027 | 0.0032 |
Punctate Nuclear | 0.0018 | 0.0021 | 0.0033 | 0.0008 | 0.0016 | 0.0035 | 0.0071 | 0.0017 | 0.0038 | 0.0015 | 0.0009 | 0.0053 | 0.0038 | 0.0011 | 0.003 | 0.0004 | 0.005 | 0.0025 |
Vacuole | 0.0031 | 0.0011 | 0.0092 | 0.0016 | 0.013 | 0.0006 | 0.0011 | 0.0005 | 0.0008 | 0.0026 | 0.0033 | 0.0003 | 0.0013 | 0.0011 | 0.0031 | 0.0004 | 0.0022 | 0.0011 |
Vacuole Periphery | 0.0009 | 0.0002 | 0.0013 | 0.0007 | 0.0021 | 0.0001 | 0.0007 | 0.0001 | 0.0003 | 0.0009 | 0.0014 | 0.0002 | 0.0008 | 0.0001 | 0.0009 | 0.0001 | 0.0005 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 4.4048 | 5.9064 | 6.209 | 6.4307 | 1.0708 | 7.1539 | 4.6034 | 6.8858 | 6.9256 | 6.3229 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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