Standard name
Human Ortholog
Description Protein kinase of unknown cellular role; putative serine/threonine kinase; expression is induced during mild heat stress; deletion mutants are hypersensitive to copper sulphate and resistant to sorbate; interacts with an N-terminal fragment of Sst2p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.95 0.96 0.96 0.98 0.9 0.88 0.88 0.67 0.79 0.55 0.72 0.99 0.99 0.99 0.94 0.97 0.97 0.7 0.78 0.52 0.87 0.84 0.85
Endoplasmic Reticulum 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0.06 0.07 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.05 0.09 0.1 0.37 0.22 0.52 0.24 0 0 0 0 0 0 0.15 0.1 0.23 0 0.05 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 1 1 1 1 0 0 0 0 0 1 5 4 2 3 6 7
Bud 3 0 0 0 2 1 2 3 2 4 3 0 0 0 0 0 0 1 1 1 1 11 10
Bud Neck 0 2 1 0 3 2 4 0 0 2 2 3 1 0 5 2 0 1 3 1 0 1 3
Bud Site 1 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0
Cell Periphery 5 2 1 1 5 2 1 0 1 0 4 2 1 5 2 1 3 2 3 1 0 3 5
Cytoplasm 362 321 80 114 178 248 221 66 140 96 179 365 478 623 335 253 223 283 271 53 149 405 442
Endoplasmic Reticulum 2 10 1 0 7 6 3 5 2 3 6 2 1 0 21 18 16 13 7 2 1 2 2
Endosome 1 0 0 1 0 5 1 0 0 0 5 1 2 1 6 0 1 5 1 2 1 4 4
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 1 2 0 0 0 1 0 0 2 1
Mitochondria 17 3 0 0 9 25 24 36 39 91 60 0 0 0 13 3 2 61 35 23 7 23 19
Nucleus 0 1 1 0 1 2 2 2 2 1 2 1 1 1 1 1 1 0 0 0 0 3 0
Nuclear Periphery 1 2 0 0 0 0 1 0 2 0 0 0 0 0 1 0 0 1 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 1 0 0 0 1 1 0 0 0 1 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0 0
SpindlePole 2 0 1 1 1 4 2 0 0 6 6 1 1 1 1 1 0 1 4 0 0 5 8
Vac/Vac Membrane 5 4 0 1 1 5 7 1 10 3 7 0 0 3 11 6 3 17 6 6 2 1 8
Unique Cell Count 380 334 83 116 197 283 251 98 177 174 249 370 485 629 358 262 229 406 347 102 172 485 522
Labelled Cell Count 400 345 85 118 208 300 268 114 200 209 275 375 486 636 400 286 250 406 347 102 172 485 522


(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.8 4.2 5.3 5.8 4.6 5.0 5.2 3.7 4.5 3.8 4.2 5.3 5.3 5.5 5.7 5.6 5.0 5.3 5.1 5.5
Std Deviation (1e-4) 0.7 0.7 1.7 1.6 1.0 1.8 1.5 0.9 1.4 0.9 1.1 1.2 1.3 1.5 1.1 1.8 1.0 1.6 1.4 1.6
Intensity Change (Log2) 0.13 -0.21 -0.09 -0.04 -0.5 -0.22 -0.47 -0.33 0.01 0.01 0.06 0.1 0.08 -0.07 -0.01 -0.04 0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.27 -0.2808 -0.2057 -0.1874 0.0728 -0.3839 -0.6591 -0.4987 -0.3714 -0.7108 -0.4833 -0.5075 -1.1643 -0.7281 -1.0387 -0.8984 -0.8518 -0.7486
Actin 0.01 0.0006 0.0246 0.0182 0.0523 0.0004 0.0312 0.0003 0.0028 0.0026 0.0039 0.0018 0.0331 0.0009 0.0097 0.0003 0.0159 0.0146
Bud 0.002 0.0012 0.0034 0.0004 0.001 0.0008 0.0066 0.0138 0.0066 0.0046 0.0005 0.0005 0.0007 0.0067 0.0016 0.0001 0.0001 0.0003
Bud Neck 0.011 0.0002 0.0044 0.0009 0.001 0.0009 0.0081 0.0001 0.0004 0.0023 0.0011 0.0002 0.0026 0.0001 0.0003 0.0001 0.0003 0.0163
Bud Periphery 0.0056 0.0038 0.0056 0.0009 0.0015 0.0013 0.0053 0.0019 0.0074 0.0019 0.0014 0.0004 0.0023 0.0056 0.0026 0.0004 0.0003 0.0005
Bud Site 0.0074 0.0071 0.0158 0.0002 0.0091 0.0001 0.0164 0.0095 0.0125 0.0041 0.0007 0.0004 0.0015 0.0041 0.0024 0 0.0003 0.0008
Cell Periphery 0.0014 0.0003 0.001 0.0001 0.0004 0.0001 0.0005 0.0003 0.0003 0.0005 0.0002 0.0001 0.0003 0.0003 0.0002 0.0001 0.0001 0.0001
Cytoplasm 0.0329 0.0338 0.0392 0.0473 0.0304 0.0857 0.0196 0.0712 0.0278 0.0343 0.0671 0.0578 0.0832 0.1721 0.1608 0.2105 0.1748 0.2129
Cytoplasmic Foci 0.0203 0.0073 0.0226 0.011 0.0287 0.0229 0.0178 0.0045 0.0219 0.0324 0.0038 0.0049 0.0183 0.0054 0.0163 0.0026 0.0134 0.0131
Eisosomes 0.0006 0.0003 0.0005 0.0002 0.0008 0.0002 0.0023 0.0002 0.0003 0.0002 0.0001 0.0001 0.0007 0.0003 0.0003 0.0001 0.0002 0.0001
Endoplasmic Reticulum 0.0099 0.0017 0.0029 0.0018 0.0027 0.0009 0.0015 0.0017 0.001 0.0166 0.0016 0.0007 0.0056 0.003 0.0052 0.0018 0.0048 0.004
Endosome 0.0179 0.0013 0.0117 0.0131 0.0585 0.0037 0.0179 0.002 0.0107 0.0131 0.0232 0.0024 0.0199 0.0019 0.0145 0.0007 0.0089 0.0074
Golgi 0.0068 0.0003 0.0069 0.0199 0.0214 0.0005 0.0089 0.0004 0.0058 0.0081 0.0078 0.0038 0.0057 0.0002 0.002 0.0001 0.0055 0.0014
Lipid Particles 0.0153 0.0008 0.0097 0.0032 0.0411 0.0013 0.0195 0.0007 0.0092 0.0051 0.0057 0.0013 0.007 0.0009 0.0073 0.0002 0.0148 0.0041
Mitochondria 0.0166 0.0011 0.0114 0.0445 0.0447 0.0006 0.007 0.0003 0.0131 0.0035 0.0047 0.008 0.0017 0.0005 0.0067 0.0003 0.001 0.0052
None 0.8187 0.9338 0.8121 0.8306 0.6598 0.8691 0.8074 0.8885 0.8646 0.8522 0.8699 0.9053 0.7912 0.7935 0.7521 0.7802 0.7432 0.7036
Nuclear Periphery 0.0034 0.0005 0.0025 0.0011 0.0016 0.0015 0.0028 0.0004 0.0004 0.0076 0.0011 0.0008 0.0094 0.0008 0.0023 0.0007 0.0044 0.0054
Nucleolus 0.0007 0.0005 0.0011 0.0002 0.0013 0.0003 0.0015 0.0004 0.0006 0.0008 0.0003 0.0037 0.0003 0.0003 0.0005 0.0001 0.0003 0.0008
Nucleus 0.0015 0.0008 0.0014 0.0006 0.0011 0.0043 0.0037 0.0015 0.0019 0.0018 0.0005 0.0018 0.0019 0.0011 0.0017 0.0006 0.0013 0.0022
Peroxisomes 0.0122 0.0013 0.0097 0.0026 0.026 0.0011 0.0129 0.0001 0.008 0.0033 0.0008 0.0004 0.0088 0.0002 0.0061 0.0001 0.0027 0.0032
Punctate Nuclear 0.0018 0.0021 0.0033 0.0008 0.0016 0.0035 0.0071 0.0017 0.0038 0.0015 0.0009 0.0053 0.0038 0.0011 0.003 0.0004 0.005 0.0025
Vacuole 0.0031 0.0011 0.0092 0.0016 0.013 0.0006 0.0011 0.0005 0.0008 0.0026 0.0033 0.0003 0.0013 0.0011 0.0031 0.0004 0.0022 0.0011
Vacuole Periphery 0.0009 0.0002 0.0013 0.0007 0.0021 0.0001 0.0007 0.0001 0.0003 0.0009 0.0014 0.0002 0.0008 0.0001 0.0009 0.0001 0.0005 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 4.4048 5.9064 6.209 6.4307 1.0708 7.1539 4.6034 6.8858 6.9256 6.3229
Translational Efficiency

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Protein kinase of unknown cellular role; putative serine/threonine kinase; expression is induced during mild heat stress; deletion mutants are hypersensitive to copper sulphate and resistant to sorbate; interacts with an N-terminal fragment of Sst2p
Localization
Cell Percentages cytoplasm (34%), ER (1%), mixed (38%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Iks1

Iks1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Iks1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available