Standard name | |
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Human Ortholog | |
Description | Acetylglutamate synthase (glutamate N-acetyltransferase); mitochondrial enzyme that catalyzes the first step in the biosynthesis of the arginine precursor ornithine; forms a complex with Arg5,6p |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 2 | 3 | 3 | 1 | 0 | 3 | – | 4 | 1 | 0 | 0 | 1 | 6 | 0 | 3 | 1 | 4 | 0 | 7 | 4 | 0 | 0 | 3 |
Bud | 5 | 5 | 1 | 11 | 3 | 9 | 8 | – | 8 | 7 | 15 | 23 | 0 | 3 | 0 | 3 | 3 | 7 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 2 | – | 0 | 3 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 3 | 0 | 0 | 0 | 0 | 1 | 2 | – | 1 | 5 | 4 | 6 | 0 | 0 | 0 | 3 | 1 | 1 | – | – | – | – | – | – |
Cell Periphery | 6 | 8 | 3 | 8 | 1 | 0 | 5 | – | 1 | 2 | 1 | 3 | 1 | 10 | 3 | 7 | 11 | 12 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4 | 6 | 3 | 10 | 20 | 16 | 15 | – | 10 | 13 | 11 | 11 | 2 | 9 | 6 | 9 | 19 | 14 | 1 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 1 | 3 | 4 | 4 | 4 | 3 | 2 | – | 1 | 0 | 1 | 5 | 3 | 5 | 1 | 9 | 13 | 6 | 0 | 1 | 2 | 0 | 0 | 1 |
Endosome | 28 | 24 | 14 | 14 | 54 | 24 | 18 | – | 2 | 3 | 1 | 5 | 3 | 22 | 12 | 37 | 10 | 30 | 27 | 32 | 13 | 5 | 11 | 9 |
Golgi | 2 | 8 | 20 | 21 | 20 | 0 | 4 | – | 0 | 1 | 0 | 0 | 3 | 13 | 3 | 41 | 51 | 24 | 14 | 20 | 4 | 3 | 6 | 11 |
Mitochondria | 291 | 178 | 75 | 164 | 132 | 269 | 190 | – | 192 | 236 | 235 | 302 | 11 | 49 | 18 | 25 | 25 | 26 | 249 | 195 | 129 | 28 | 65 | 114 |
Nucleus | 7 | 5 | 3 | 3 | 3 | 8 | 12 | – | 8 | 7 | 4 | 3 | 1 | 2 | 0 | 4 | 2 | 5 | 0 | 1 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 6 | 4 | 0 | 0 | 3 | 3 | 8 | – | 4 | 7 | 5 | 10 | 0 | 0 | 0 | 3 | 1 | 0 | 4 | 3 | 0 | 0 | 0 | 2 |
Nucleolus | 6 | 1 | 1 | 0 | 5 | 13 | 6 | – | 3 | 1 | 2 | 7 | 0 | 0 | 0 | 4 | 1 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 17 | 12 | 11 | 4 | 5 | 4 | 3 | – | 3 | 6 | 4 | 7 | 0 | 5 | 12 | 27 | 12 | 30 | 23 | 18 | 16 | 2 | 4 | 8 |
SpindlePole | 13 | 7 | 14 | 6 | 20 | 25 | 16 | – | 18 | 30 | 10 | 50 | 0 | 4 | 3 | 39 | 21 | 39 | 5 | 2 | 1 | 1 | 1 | 1 |
Vac/Vac Membrane | 35 | 44 | 26 | 11 | 31 | 20 | 16 | – | 5 | 9 | 6 | 8 | 4 | 19 | 10 | 24 | 26 | 34 | 4 | 18 | 6 | 1 | 8 | 14 |
Unique Cell Count | 328 | 234 | 131 | 205 | 238 | 331 | 257 | 219 | 274 | 252 | 344 | 23 | 116 | 54 | 185 | 159 | 174 | 339 | 306 | 182 | 46 | 104 | 170 | |
Labelled Cell Count | 424 | 307 | 178 | 259 | 302 | 395 | 310 | 260 | 331 | 299 | 442 | 29 | 147 | 68 | 238 | 197 | 238 | 339 | 306 | 182 | 46 | 104 | 170 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.4 | 6.2 | 6.8 | 6.4 | 6.8 | 5.4 | 5.7 | – | 4.5 | 4.4 | 4.1 | 4.3 | 7.0 | 6.7 | 7.0 | 7.7 | 7.9 | 7.5 | – | 5.9 | 6.8 |
Std Deviation (1e-4) | 0.8 | 1.4 | 1.1 | 1.1 | 1.5 | 1.3 | 1.7 | – | 1.4 | 1.2 | 1.4 | 1.5 | 0.9 | 1.0 | 1.8 | 1.2 | 1.5 | 1.6 | – | 1.1 | 1.1 |
Intensity Change (Log2) | – | – | – | -0.08 | 0.01 | -0.34 | -0.26 | – | -0.6 | -0.61 | -0.72 | -0.66 | 0.04 | -0.03 | 0.04 | 0.17 | 0.22 | 0.15 | – | -0.19 | 0.01 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2.2 | 0 | 0 | 1.8 | 1.8 |
Cytoplasm | 1.2 | 2.3 | 1.2 | 1.6 | – | 0 | 1.2 | 0 | 0 | 0 | 2.0 | 0 | 0 | 3.1 | 2.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.8 | 1.8 | 0 |
Endosome | -1.2 | 2.8 | -1.2 | -1.2 | – | 0 | 0 | 0 | 0 | 0 | 1.8 | 2.1 | 2.2 | -1.4 | 1.6 |
Golgi | -1.4 | -2.0 | -7.3 | -5.3 | – | 0 | 0 | 0 | 0 | 0 | -0.9 | -1.8 | 1.5 | 3.3 | -0.4 |
Mitochondria | 4.5 | -0.3 | 5.3 | 3.3 | – | 0 | 0 | 0 | 0 | -0.8 | -2.4 | -3.0 | -8.2 | -7.4 | -7.8 |
Nucleus | 0 | 0 | 0 | 1.1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | -2.8 | -2.8 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | -1.3 | 2.6 | 1.7 | -0.3 | 2.2 |
SpindlePole | -2.9 | -0.7 | -1.1 | -1.6 | – | 0 | 0 | 0 | 0 | 0 | -2.2 | 0 | 2.4 | 0.7 | 2.7 |
Vacuole | -4.1 | -1.7 | -4.5 | -4.1 | – | -5.6 | -5.5 | -5.9 | -6.6 | 0 | -0.7 | -0.2 | -1.6 | -0.8 | -0.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.8386 | 2.4442 | 1.9855 | 2.0647 | 1.9813 | 2.3321 | 2.0122 | 2.1166 | 1.9104 | 2.0589 | 1.7065 | 2.1118 | 2.5959 | 2.4454 | 2.1218 | 2.2801 | 2.1548 | 2.2567 |
Actin | 0.0237 | 0.004 | 0.0111 | 0.0002 | 0.0029 | 0.0028 | 0.0111 | 0.0119 | 0.004 | 0.0038 | 0.0229 | 0.0032 | 0.059 | 0.0168 | 0.0215 | 0.0429 | 0.0008 | 0.0075 |
Bud | 0.0003 | 0.0008 | 0.0002 | 0 | 0.0015 | 0.0001 | 0.0002 | 0.0009 | 0.0003 | 0.0002 | 0.0025 | 0.0002 | 0.0024 | 0.0061 | 0.0024 | 0.0006 | 0.0001 | 0.0005 |
Bud Neck | 0.0004 | 0.0005 | 0.0007 | 0.0001 | 0.0003 | 0.0002 | 0.0005 | 0.0003 | 0.0002 | 0.0003 | 0.0003 | 0.0002 | 0.0026 | 0.0003 | 0.0004 | 0.0116 | 0 | 0.0006 |
Bud Periphery | 0.0005 | 0.0017 | 0.0004 | 0.0001 | 0.0085 | 0.0005 | 0.0005 | 0.0018 | 0.0005 | 0.0005 | 0.0114 | 0.0005 | 0.0052 | 0.0045 | 0.0077 | 0.0006 | 0.0001 | 0.0006 |
Bud Site | 0.0015 | 0.0054 | 0.0008 | 0.0001 | 0.0007 | 0.0002 | 0.0006 | 0.0044 | 0.0008 | 0.0003 | 0.0009 | 0.0002 | 0.0055 | 0.0075 | 0.0025 | 0.0028 | 0.0001 | 0.0012 |
Cell Periphery | 0.0002 | 0.0004 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0004 | 0.0002 | 0.0002 | 0 | 0.0004 |
Cytoplasm | 0.0004 | 0.0004 | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0.002 | 0.0016 | 0.0003 | 0.0002 | 0.0019 | 0.0002 | 0.0082 | 0.0017 | 0.0003 | 0.0004 | 0 | 0.0072 |
Cytoplasmic Foci | 0.0082 | 0.0189 | 0.0154 | 0.0026 | 0.0148 | 0.0058 | 0.0177 | 0.0206 | 0.0087 | 0.0086 | 0.0036 | 0.006 | 0.0312 | 0.0168 | 0.0122 | 0.0469 | 0.0009 | 0.013 |
Eisosomes | 0.0004 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0004 | 0.0002 | 0.0003 | 0.0003 | 0.0002 | 0.0006 | 0.0003 | 0.0003 | 0.0004 | 0.0001 | 0.0004 |
Endoplasmic Reticulum | 0.0005 | 0.0003 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0014 | 0.0017 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0035 | 0.0004 | 0.0002 | 0.0002 | 0 | 0.0002 |
Endosome | 0.0278 | 0.0427 | 0.0254 | 0.0209 | 0.0053 | 0.0202 | 0.0423 | 0.044 | 0.0156 | 0.0089 | 0.0073 | 0.0111 | 0.0352 | 0.0279 | 0.0242 | 0.014 | 0.0034 | 0.0299 |
Golgi | 0.0307 | 0.0531 | 0.0252 | 0.0223 | 0.0095 | 0.0415 | 0.0407 | 0.0534 | 0.0274 | 0.0266 | 0.0095 | 0.0235 | 0.0437 | 0.0646 | 0.0562 | 0.0211 | 0.0101 | 0.0861 |
Lipid Particles | 0.0274 | 0.0292 | 0.0256 | 0.0031 | 0.0181 | 0.0218 | 0.0343 | 0.028 | 0.0055 | 0.0015 | 0.0065 | 0.0096 | 0.0523 | 0.0218 | 0.0171 | 0.0713 | 0.0011 | 0.009 |
Mitochondria | 0.8407 | 0.7776 | 0.8388 | 0.9115 | 0.8809 | 0.8684 | 0.7853 | 0.7713 | 0.9056 | 0.9318 | 0.8271 | 0.9138 | 0.6718 | 0.762 | 0.8048 | 0.7079 | 0.9712 | 0.8035 |
None | 0.0011 | 0.0002 | 0.0004 | 0 | 0.0004 | 0.0001 | 0.0038 | 0.0044 | 0.0002 | 0.0001 | 0.03 | 0.0002 | 0.0048 | 0.0008 | 0.0002 | 0.0016 | 0 | 0.0001 |
Nuclear Periphery | 0.0012 | 0.0008 | 0.001 | 0 | 0.001 | 0.0001 | 0.0076 | 0.0031 | 0.0001 | 0 | 0.002 | 0.0019 | 0.008 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 |
Nucleolus | 0.0004 | 0.0004 | 0.0002 | 0.0001 | 0.0003 | 0.0003 | 0.0006 | 0.0006 | 0.0001 | 0 | 0.002 | 0.0002 | 0.0006 | 0.0003 | 0.0002 | 0.0018 | 0 | 0.0003 |
Nucleus | 0.0005 | 0.0002 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0025 | 0.0009 | 0.0001 | 0 | 0.0076 | 0.0005 | 0.0045 | 0.0003 | 0.0001 | 0.0002 | 0 | 0.0001 |
Peroxisomes | 0.0297 | 0.0533 | 0.0475 | 0.036 | 0.0507 | 0.0332 | 0.0386 | 0.0431 | 0.028 | 0.0152 | 0.0548 | 0.0259 | 0.0485 | 0.0643 | 0.045 | 0.0697 | 0.0115 | 0.0351 |
Punctate Nuclear | 0.0008 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0.0035 | 0.0003 | 0.0001 | 0 | 0.007 | 0.0006 | 0.0055 | 0.0001 | 0.0001 | 0.0023 | 0 | 0.0001 |
Vacuole | 0.0017 | 0.0049 | 0.0022 | 0.0011 | 0.0009 | 0.0015 | 0.0029 | 0.003 | 0.0008 | 0.0004 | 0.0009 | 0.0007 | 0.0039 | 0.0015 | 0.0024 | 0.0015 | 0.0001 | 0.0017 |
Vacuole Periphery | 0.0019 | 0.0048 | 0.0035 | 0.0015 | 0.0033 | 0.0028 | 0.0035 | 0.004 | 0.0012 | 0.0008 | 0.0012 | 0.0012 | 0.0029 | 0.0015 | 0.0021 | 0.0019 | 0.0005 | 0.0024 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 10.2345 | 9.7208 | 6.3646 | 9.7694 | 12.4401 | 10.8681 | 10.2623 | 11.3163 | 9.1661 | 11.1544 |
Translational Efficiency | 0.4404 | 0.46 | 0.5892 | 0.521 | 0.3223 | 0.4646 | 0.3456 | 0.3742 | 0.583 | 0.4321 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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