Standard name
Human Ortholog
Description Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY2; may be involved in detoxification of hydrophobic compounds; PRY1 has a paralog, PRY2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.1 0.1 0.19 0.26 0.18 0.18 0.14 0.19 0.16 0.18 0.21 0.21 0.07 0.56 0.79 0.14 0.09 0.14 0 0 0 0 0 0.07
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.08 0.06 0.06 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0.06 0.07 0.05 0 0.06 0.08
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.1 0.12 0 0 0 0.05 0 0 0.05 0 0 0 0.07 0 0 0 0.07 0 0 0 0 0 0 0
Nucleus 0.2 0.2 0.28 0.37 0.26 0.34 0.36 0.35 0.34 0.26 0.25 0.25 0.29 0.18 0.13 0.45 0.43 0.45 0 0 0 0.05 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.68 0.68 0.6 0.55 0.71 0.67 0.66 0.7 0.65 0.67 0.68 0.72 0.74 0.38 0.26 0.49 0.52 0.51 0.81 0.82 0.8 0.73 0.74 0.72
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 1 0 0 0 0 1 0 0 1 0 1 0 0 0 0 0 0 0 7 2 0 1 1
Bud Neck 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 18 50 30 37 37 45 58 78 56 71 64 72 5 93 190 20 10 11 6 7 2 2 3 9
Endoplasmic Reticulum 0 0 0 0 0 0 1 1 1 2 0 0 0 0 1 3 2 0 0 0 1 0 0 1
Endosome 15 31 9 5 8 5 6 5 6 4 0 2 4 4 1 4 2 2 12 37 8 1 6 11
Golgi 0 0 0 0 2 0 0 0 1 5 1 0 2 0 0 0 0 3 0 3 0 0 1 0
Mitochondria 19 60 2 0 3 12 5 3 19 13 10 11 5 0 0 2 7 0 2 9 3 1 1 6
Nucleus 37 100 45 52 53 85 151 147 118 104 78 87 21 30 32 64 46 35 6 15 5 3 3 1
Nuclear Periphery 1 10 1 0 0 0 1 0 2 2 3 1 1 0 0 0 3 3 4 2 1 3 2 3
Nucleolus 2 8 0 1 2 1 1 3 6 0 3 2 1 0 0 1 4 1 2 3 0 0 0 2
Peroxisomes 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
SpindlePole 2 6 3 1 3 0 0 1 1 1 0 2 0 1 1 0 0 0 1 5 2 0 0 1
Vac/Vac Membrane 123 333 95 77 146 169 274 293 230 268 209 250 53 62 63 69 55 40 164 434 142 47 80 100
Unique Cell Count 181 489 159 141 206 251 415 421 352 402 307 345 72 165 241 142 106 78 202 531 177 65 108 140
Labelled Cell Count 217 600 186 173 254 317 498 532 441 471 369 430 92 190 288 163 129 95 202 531 177 65 108 140


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.4 6.2 7.8 8.4 10.3 8.9 10.4 11.6 11.9 13.9 12.8 13.2 7.0 5.9 5.2 8.5 9.6 11.3 7.6 7.1 6.5
Std Deviation (1e-4) 1.7 1.8 2.6 2.9 3.8 3.5 3.8 4.5 5.3 5.8 5.3 5.7 2.2 1.6 1.3 2.9 3.2 4.2 2.7 2.5 1.6
Intensity Change (Log2) – – – 0.11 0.4 0.18 0.42 0.57 0.61 0.83 0.71 0.76 -0.16 -0.41 -0.58 0.13 0.3 0.53 -0.05 -0.14 -0.26


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.5 -0.2 -0.2 -1.5 -0.1 -0.8 -0.3 0.5 0.5 -2.3 7.0 11.8 -1.1 -2.1 -0.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome -0.9 -0.8 -2.0 0 0 0 0 0 0 0 -1.5 0 -1.2 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 1.9 0 0 2.2 0 0 0 0 0 0 0 0 0
Nucleus 1.6 -0.6 1.2 1.8 1.5 1.2 -0.6 -0.7 -0.7 0.1 -2.2 -3.7 3.0 2.5 2.5
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -0.9 2.2 1.6 1.4 2.2 1.2 1.5 1.8 2.9 2.0 -4.0 -6.7 -1.9 -1.3 -1.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.8454 5.3377 3.6036 3.3615 3.2923 4.3261 6.1254 6.3324 6.2558 4.9111 5.2801 6.9045 2.0232 3.6957 2.5469 1.9695 3.0405 2.9383
Actin 0.0351 0 0.0217 0 0.0035 0 0.0144 0 0.0071 0.0004 0.0025 0.0038 0.0398 0.0043 0.0125 0.0071 0.0025 0.0061
Bud 0.0005 0.0008 0.0005 0.0002 0.002 0.0002 0.0008 0.0013 0.0012 0.0007 0.0003 0.0008 0.0009 0.0018 0.0016 0.0008 0.0003 0.0004
Bud Neck 0.0029 0.0009 0.001 0.0008 0.0029 0.0019 0.0011 0.0008 0.0016 0.0015 0.0007 0.0019 0.0051 0.0021 0.0013 0.001 0.0018 0.0018
Bud Periphery 0.0012 0.0013 0.0016 0.0006 0.0036 0.0009 0.0018 0.0037 0.0043 0.0016 0.0005 0.0019 0.0017 0.0027 0.0026 0.0026 0.0008 0.0014
Bud Site 0.0171 0.0017 0.0023 0.0006 0.0137 0.0007 0.0022 0.0016 0.0018 0.0019 0.001 0.0013 0.0063 0.0043 0.0028 0.0014 0.0013 0.0009
Cell Periphery 0.0059 0.0045 0.0056 0.0034 0.0044 0.0059 0.0051 0.0071 0.0048 0.0038 0.0027 0.0063 0.0024 0.0048 0.0052 0.0015 0.0027 0.0041
Cytoplasm 0.0023 0.0065 0.0067 0.0035 0.0044 0.0013 0.0058 0.0033 0.002 0.002 0.0011 0.0038 0.0051 0.0165 0.0136 0.0039 0.0038 0.0023
Cytoplasmic Foci 0.0166 0.0065 0.0165 0.0056 0.0114 0.0054 0.0282 0.0066 0.0128 0.0197 0.0408 0.0231 0.027 0.015 0.0244 0.0067 0.0218 0.0167
Eisosomes 0.0004 0 0.0002 0 0.0001 0 0.0002 0 0.0001 0 0 0 0.0007 0 0.0001 0.0002 0.0001 0.0001
Endoplasmic Reticulum 0.002 0.0035 0.0018 0.001 0.0015 0.0011 0.0021 0.0019 0.0016 0.0013 0.0007 0.002 0.0052 0.0023 0.0038 0.0009 0.001 0.0022
Endosome 0.1529 0.0836 0.1197 0.1484 0.1237 0.1452 0.1579 0.1222 0.1733 0.1455 0.1812 0.2007 0.2602 0.144 0.2028 0.1941 0.1445 0.2276
Golgi 0.0141 0.0027 0.0096 0.0027 0.005 0.0056 0.0184 0.0047 0.0136 0.0084 0.0216 0.0138 0.0293 0.0059 0.0144 0.0049 0.034 0.0098
Lipid Particles 0.0846 0.0194 0.0801 0.0195 0.0384 0.0507 0.0991 0.0528 0.065 0.0288 0.0626 0.0756 0.0706 0.046 0.0448 0.023 0.0403 0.0502
Mitochondria 0.0486 0.0139 0.0897 0.0272 0.0086 0.0147 0.0643 0.0405 0.0999 0.0308 0.0315 0.0117 0.0623 0.0124 0.0774 0.0211 0.0209 0.0156
None 0.0174 0.0004 0.0015 0.0043 0.0552 0.0002 0.028 0.0006 0.0097 0.0322 0.0006 0.0004 0.0129 0.0036 0.0176 0.0255 0.0039 0.0008
Nuclear Periphery 0.0051 0.0076 0.0182 0.0023 0.0031 0.0036 0.0043 0.0046 0.0108 0.0037 0.001 0.0057 0.0141 0.01 0.008 0.002 0.0009 0.006
Nucleolus 0.0293 0.0044 0.0142 0.0103 0.0368 0.0075 0.0167 0.0055 0.012 0.0115 0.0043 0.0129 0.0073 0.0178 0.0128 0.0047 0.0092 0.0147
Nucleus 0.0076 0.0036 0.0042 0.0025 0.0466 0.0034 0.0064 0.005 0.0129 0.0037 0.002 0.0058 0.0064 0.0153 0.0104 0.0026 0.0026 0.0082
Peroxisomes 0.027 0.0006 0.011 0.0015 0.007 0.0012 0.0221 0.0026 0.0059 0.003 0.0042 0.0089 0.0196 0.0041 0.0143 0.0019 0.0439 0.0072
Punctate Nuclear 0.0061 0.0002 0.0005 0.0001 0.0019 0.0001 0.0017 0.0001 0.0004 0.0017 0.0006 0.0004 0.0052 0.0014 0.0009 0.0002 0.0004 0.0021
Vacuole 0.3409 0.5726 0.3962 0.5936 0.4589 0.5655 0.3421 0.5042 0.3815 0.5392 0.4973 0.4278 0.2771 0.5097 0.368 0.5233 0.5271 0.4638
Vacuole Periphery 0.1825 0.265 0.1973 0.172 0.1675 0.1848 0.1773 0.2307 0.1776 0.1589 0.143 0.1912 0.1407 0.176 0.1608 0.1708 0.1361 0.1579

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 44.7492 16.4396 121.9889 72.8614 19.0748 139.6872 47.927 75.9137 76.1294 34.285
Translational Efficiency 1.4572 1.4514 1.6124 1.5848 1.642 1.342 1.1858 1.9884 1.9544 1.5352

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✔
Standard name
Human Ortholog
Description Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY2; may be involved in detoxification of hydrophobic compounds; PRY1 has a paralog, PRY2, that arose from the whole genome duplication
Localization
Cell Percentages vacuole (60%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Pry1

Pry1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pry1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available