Standard name
Human Ortholog
Description Essential RNA-binding G protein effector of mating response pathway; ligand-activated RNA-binding protein that delivers RNAs involved in polarization and perpetualizing mating signal to shmoo tip during pheromone signaling; Scp160p-mediated RNA trafficking essential for chemotropism and successful mating; mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0.06 0 0 0 0 0.07 0.08 0.09 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0.05 0 0.05 0 0.08 0.06 0.06 0.07 0 0 0.07 0.11 0.05 0.15 0 0 0 0 0 0
Cytoplasm 0.62 0.65 0.23 0.11 0.11 0.19 0.22 0.14 0.24 0.21 0.26 0.16 0.73 0.71 0.9 0.87 0.92 0.9 0.24 0.28 0.05 0.07 0.09 0.1
Endoplasmic Reticulum 0.47 0.39 0.74 0.67 0.65 0.72 0.69 0.76 0.68 0.68 0.61 0.59 0.32 0.35 0.14 0 0 0 0.69 0.66 0.83 0.68 0.75 0.74
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0.1 0.32 0.32 0.11 0.1 0.08 0.06 0.07 0.07 0.15 0 0 0 0 0 0 0 0 0.05 0.16 0.09 0.08
Mitochondria 0 0 0 0 0 0 0 0.05 0.05 0.07 0.12 0.14 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 15 12 11 11 20 17 24 24 20 0 0 0 1 1 0 5 3 9 1 4 3
Bud 2 0 0 0 0 1 1 2 0 0 1 1 0 0 0 1 0 1 1 1 1 1 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 7 11 10 17 11 15 18 20 17 16 17 4 4 11 16 8 18 2 0 1 2 2 2
Cytoplasm 310 196 77 27 34 55 65 70 62 64 67 41 80 80 136 125 141 111 135 90 16 12 21 24
Endoplasmic Reticulum 234 117 244 157 204 204 204 389 176 205 159 149 35 40 21 1 5 3 382 212 287 122 176 178
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 1 0 0 0 0 3
Golgi 9 0 32 75 101 30 29 43 15 22 19 37 1 0 0 3 4 0 5 1 17 28 20 19
Mitochondria 0 0 1 5 0 4 2 27 12 20 31 35 0 0 0 2 5 0 0 0 5 0 1 2
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 2 0 2 2 4 0 0 0 0 0 0 1 1 0 0 0 1
Nucleolus 0 0 0 0 0 1 2 4 0 1 0 3 0 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 2 0 0 2 8 6 3 3 3 3 2 3 3 3 0 3 2 0 4 1 0 3 2 2
Unique Cell Count 499 300 331 235 314 283 297 509 258 301 261 253 110 113 151 144 154 123 554 320 345 180 235 241
Labelled Cell Count 560 320 366 291 376 323 332 578 305 358 321 310 123 127 168 155 166 134 554 320 345 180 235 241


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 73.3 83.1 67.0 58.7 56.9 51.6 48.6 45.6 44.2 41.8 39.4 39.8 51.3 51.3 57.4 45.9 56.1 68.2 59.7 68.2 73.5
Std Deviation (1e-4) 13.7 15.1 12.6 12.7 11.1 10.1 10.6 9.8 9.1 9.2 8.2 9.2 13.6 13.6 14.4 17.7 21.2 21.4 15.5 16.4 17.6
Intensity Change (Log2) – – – -0.19 -0.24 -0.38 -0.46 -0.56 -0.6 -0.68 -0.77 -0.75 -0.39 -0.39 -0.22 -0.54 -0.25 0.03 -0.17 0.03 0.13


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 4.3 3.2 3.2 3.1 3.3 4.4 4.9 5.3 4.9 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.6 1.3 0.4 1.1 0.2 2.4 1.4 1.6 1.9 0 0 1.9 3.4 1.0 4.4
Cytoplasm -3.6 -4.2 -1.2 -0.4 -3.5 0.2 -0.6 0.7 -2.1 9.4 9.1 13.7 12.9 14.1 12.9
Endoplasmic Reticulum -1.8 -2.4 -0.5 -1.4 0.9 -1.5 -1.6 -3.3 -3.8 -7.9 -7.3 -12.2 -14.6 -14.5 -13.6
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 6.7 7.1 0.4 0 -0.6 -1.7 -1.1 -1.0 1.8 -3.0 -3.4 -4.0 -2.9 -2.8 -3.6
Mitochondria 0 0 0 0 3.9 3.6 4.4 6.2 6.7 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0269 0.0152 0.0242 0.0657 0.0799 0.0212 0.0392 0.0169 0.0262 0.003 0.0068 0.026 0.0417 0.0037 0.0001 0.0859 0.0763 0.0007
Bud 0.0005 0.0007 0.0004 0.009 0.0015 0.002 0.0015 0.0031 0.0006 0.0003 0.0005 0.0002 0.0003 0 0 0.0057 0.0066 0
Bud Neck 0.0007 0.0017 0.0029 0.0047 0.0069 0.0071 0.001 0.0013 0.0012 0.0012 0.0019 0.003 0.0002 0.0003 0 0.0177 0.0091 0.0003
Bud Periphery 0.0011 0.0012 0.0007 0.0082 0.0012 0.0024 0.0023 0.0029 0.0013 0.0001 0.0018 0.0002 0.0005 0 0 0.0041 0.0043 0
Bud Site 0.0011 0.0026 0.0004 0.0434 0.0071 0.0003 0.0019 0.018 0.0021 0 0.0001 0.0001 0.0003 0 0 0.0005 0.0007 0
Cell Periphery 0.0059 0.041 0.0159 0.011 0.0269 0.0076 0.0116 0.0142 0.01 0.001 0.0024 0.0018 0.0002 0.0002 0.0003 0.0038 0.0153 0.0005
Cytoplasm 0.1193 0.2668 0.1834 0.1795 0.1888 0.1398 0.079 0.1923 0.1526 0.1288 0.2069 0.0677 0.0429 0.5273 0.0007 0.6427 0.2059 0.0032
Cytoplasmic Foci 0.0243 0.0062 0.0097 0.0091 0.0249 0.0032 0.0143 0.0084 0.0089 0.001 0.0053 0.0035 0.0374 0.0029 0 0.0104 0.0049 0.0001
Eisosomes 0.0006 0.0028 0.0009 0.002 0.0045 0.0018 0.0024 0.0007 0.001 0.0001 0.0003 0.0006 0.0022 0 0 0.0001 0.0085 0.0001
Endoplasmic Reticulum 0.3522 0.4243 0.5165 0.3151 0.231 0.4218 0.3952 0.5549 0.5998 0.7918 0.4782 0.5797 0.0073 0.3342 0.9357 0.0682 0.2825 0.5036
Endosome 0.0244 0.0116 0.0139 0.0354 0.0249 0.0227 0.0365 0.0242 0.0205 0.0066 0.072 0.0253 0.0606 0.0047 0.0006 0.0651 0.0321 0.0025
Golgi 0.0123 0.0248 0.0314 0.0361 0.0257 0.0185 0.0264 0.0359 0.0312 0.0086 0.044 0.0438 0.0109 0.0013 0.0004 0.0292 0.0859 0.0042
Lipid Particles 0.0182 0.0214 0.0195 0.0129 0.0315 0.0081 0.0167 0.0146 0.0122 0.0003 0.0025 0.0107 0.0479 0.0009 0 0.0014 0.0041 0.0002
Mitochondria 0.01 0.0047 0.0054 0.0167 0.0097 0.0027 0.0251 0.0021 0.0093 0.0006 0.0404 0.0018 0.0085 0.0001 0.0001 0.003 0.0248 0.0003
None 0.0073 0.0017 0.0059 0.0014 0.0113 0.001 0.0138 0.0005 0.0017 0.0002 0.0011 0.0004 0.6041 0.0005 0 0.0012 0.002 0.0001
Nuclear Periphery 0.3319 0.1327 0.1328 0.176 0.2411 0.3079 0.2497 0.0898 0.0984 0.0457 0.0916 0.2143 0.058 0.1158 0.0583 0.0286 0.1987 0.467
Nucleolus 0.0011 0.0053 0.0006 0.0009 0.007 0.0003 0.0019 0.0006 0.0006 0 0.0001 0.0001 0.0041 0 0 0.0003 0.0009 0
Nucleus 0.0197 0.0102 0.007 0.0095 0.0136 0.0121 0.0187 0.0039 0.0035 0.0016 0.0035 0.0035 0.0074 0.0038 0.0004 0.0074 0.0074 0.0072
Peroxisomes 0.0056 0.0002 0.0015 0.001 0.0051 0.0002 0.017 0.0001 0.0004 0 0.0001 0.0012 0.0173 0 0 0.0002 0.0004 0
Punctate Nuclear 0.0104 0.0013 0.0024 0.0013 0.0141 0.0014 0.011 0.0006 0.0006 0.0001 0.0003 0.0007 0.0401 0.0011 0 0.001 0.0009 0.0003
Vacuole 0.0123 0.0168 0.0167 0.0219 0.0199 0.0076 0.0156 0.0095 0.0079 0.0051 0.0129 0.0036 0.0029 0.002 0.0009 0.0142 0.0054 0.0012
Vacuole Periphery 0.0141 0.0069 0.0078 0.0392 0.0234 0.0103 0.0191 0.0053 0.0101 0.0038 0.0272 0.0119 0.0051 0.0011 0.0025 0.0095 0.0232 0.0086

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 239.5371 243.8384 228.0186 227.8882 228.4924 196.8738 218.9546 193.2785 170.5598 215.0478
Translational Efficiency 1.9577 1.9686 1.6803 1.8449 1.9029 2.1327 1.6143 1.5543 1.6365 1.642

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Essential RNA-binding G protein effector of mating response pathway; ligand-activated RNA-binding protein that delivers RNAs involved in polarization and perpetualizing mating signal to shmoo tip during pheromone signaling; Scp160p-mediated RNA trafficking essential for chemotropism and successful mating; mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins
Localization
Cell Percentages ER (68%)
Cell Cycle Regulation No
Subcompartmental Group ER-4

Scp160

Scp160


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Scp160-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available