Standard name
Human Ortholog
Description Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.33 0.33 0.25 0.34 0.1 0.14 0.06 0.12 – 0.06 0.07 0.05 0.35 0.38 0.26 0.18 0.22 0.24 0.28 0.26 0.25 0.11 0.15 0.21
Bud 0.22 0.23 0.28 0.21 0.27 0.21 0.17 0.18 – 0.2 0.14 0.16 0.16 0.13 0.09 0.15 0.15 0.12 0.37 0.32 0.38 0.44 0.45 0.41
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.12 0 0.18 0.24 0.18 0.29 0.13 0.11
Bud Site 0.15 0.16 0.18 0.08 0.09 0.19 0.08 0.1 – 0.1 0.06 0.07 0.08 0 0 0.17 0.14 0.14 – – – – – –
Cell Periphery 0.09 0 0 0.06 0 0 0 0 – 0 0.14 0.07 0.07 0.07 0.08 0 0 0 0 0 0 0 0 0
Cytoplasm 0.26 0.24 0.21 0.23 0.35 0.32 0.28 0.23 – 0.15 0.2 0.18 0.36 0.41 0.5 0.29 0.32 0.37 0.06 0 0 0.05 0.11 0.14
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0
Endosome 0.07 0.06 0.06 0 0.15 0 0 0 – 0 0 0 0 0 0.06 0 0.05 0.05 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.12 0.05 0.23 0.08 0.34 0.48 0.48 – 0.63 0.64 0.62 0 0 0 0.1 0.07 0.07 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.11 0.09 0.2 0.23 0.19 0.13 0.14 0.13 – 0.15 0.05 0.09 0.06 0.06 0.06 0.13 0.16 0.16 0 0 0 0 0 0
SpindlePole 0.14 0.07 0.26 0.12 0.11 0.05 0.1 0.1 – 0.12 0.06 0.09 0.26 0.15 0.13 0.27 0.24 0.18 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 117 147 36 35 12 24 10 26 – 10 13 12 216 246 176 58 44 42 124 146 41 24 58 67
Bud 76 102 40 21 33 35 28 39 – 33 26 40 97 88 64 46 31 20 161 181 62 102 172 130
Bud Neck 5 13 2 4 1 1 5 4 – 2 3 10 4 0 2 8 24 4 77 136 30 67 48 35
Bud Site 51 71 26 8 11 31 14 22 – 17 12 17 50 26 10 53 28 24 – – – – – –
Cell Periphery 30 16 4 6 3 1 3 7 – 7 27 17 45 44 54 6 2 4 12 8 2 1 3 4
Cytoplasm 91 107 30 23 42 53 47 49 – 24 39 45 225 267 345 93 65 64 25 22 7 10 42 44
Endoplasmic Reticulum 7 18 0 2 2 1 3 7 – 4 3 5 11 22 21 16 8 8 2 4 0 1 6 7
Endosome 26 28 8 3 18 3 4 5 – 1 3 6 20 28 42 12 10 8 4 12 0 7 10 4
Golgi 1 0 0 0 0 0 0 0 – 0 0 0 1 2 1 1 0 2 2 0 1 1 4 3
Mitochondria 12 53 7 23 10 57 81 102 – 104 122 159 1 1 0 31 15 12 4 16 2 3 7 9
Nucleus 11 11 5 0 5 2 2 6 – 1 4 2 6 4 0 11 6 5 0 2 1 0 0 0
Nuclear Periphery 0 2 0 0 0 0 0 0 – 0 0 1 0 0 1 1 0 0 0 0 0 0 0 0
Nucleolus 8 11 3 0 3 3 7 4 – 3 5 8 2 0 2 4 0 1 0 2 0 0 0 0
Peroxisomes 40 41 29 23 23 21 24 27 – 24 10 22 38 40 41 40 33 27 2 3 2 3 7 0
SpindlePole 48 33 37 12 13 8 17 22 – 19 11 22 160 95 93 84 49 31 12 13 5 6 10 6
Vac/Vac Membrane 3 11 2 1 2 1 1 3 – 3 3 1 8 7 23 4 1 1 1 10 3 0 1 5
Unique Cell Count 351 451 144 102 121 167 169 211 164 192 257 624 652 690 316 201 173 439 567 164 233 380 321
Labelled Cell Count 526 664 229 161 178 241 246 323 252 281 367 884 870 875 468 316 253 439 567 164 233 380 321


Bud, Bud Neck, Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.4 6.5 6.0 5.5 5.3 4.6 4.5 4.5 – 4.2 4.2 4.3 7.6 7.7 8.0 5.7 5.9 7.2 7.7 8.0 8.4
Std Deviation (1e-4) 1.5 1.3 1.3 1.3 1.4 1.6 1.5 1.3 – 1.2 1.1 1.2 1.1 1.2 1.4 1.1 1.1 1.8 1.1 1.3 1.6
Intensity Change (Log2) – – – -0.12 -0.18 -0.4 -0.44 -0.41 – -0.52 -0.52 -0.49 0.34 0.36 0.41 -0.08 -0.02 0.25 0.36 0.4 0.47


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.6 -3.2 0 0 0 – 0 0 0 2.2 2.9 0.1 -1.6 -0.7 -0.1
Bud -1.3 -0.1 0 0 0 – 0 0 0 -3.5 -4.2 -6.1 -3.4 -2.8 -3.7
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 3.7 0
Bud Site -2.3 -2.1 0 0 0 – 0 0 0 -3.6 -6.2 -8.9 -0.3 -1.0 -1.0
Cell Periphery 0 0 0 0 0 – 0 0 0 2.0 1.8 2.2 0 0 0
Cytoplasm 0.3 2.5 2.2 1.4 0.5 – -1.4 -0.1 -0.8 3.5 4.5 6.4 1.9 2.4 3.1
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0 0 0 2.7 0 0
Endosome 0 2.5 0 0 0 – 0 0 0 -1.4 -0.7 0.2 -0.9 -0.2 -0.4
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria 4.2 1.1 0 0 0 – 0 0 0 0 0 0 1.8 1.0 0.8
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0.5 -0.2 0 0 0 – 0 0 0 -5.4 -5.4 -5.6 -2.1 -0.9 -1.1
SpindlePole -2.7 -3.1 0 0 0 – 0 0 0 0 -3.2 -3.7 0.2 -0.3 -1.7
Vacuole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.7718 3.0467 3.3051 2.7919 2.6975 3.4472 4.9652 4.7889 4.8465 4.8866 4.7611 5.3143 5.0532 4.3543 4.5082 4.4431 5.1356 4.5971
Actin 0.1618 0.0941 0.0628 0.1802 0.2993 0.109 0.1971 0.0876 0.151 0.2673 0.4521 0.0983 0.1825 0.0898 0.1347 0.3371 0.3895 0.1342
Bud 0.0328 0.0807 0.259 0.1518 0.1083 0.0112 0.0292 0.0663 0.2408 0.0734 0.0458 0.0123 0.0526 0.0775 0.2949 0.0706 0.0423 0.0131
Bud Neck 0.0801 0.0078 0.0239 0.0016 0.004 0.6221 0.1321 0.0154 0.0251 0.0056 0.0266 0.6951 0.0815 0.0079 0.0132 0.0033 0.102 0.6552
Bud Periphery 0.0858 0.0846 0.4226 0.5348 0.4586 0.0352 0.0762 0.071 0.3594 0.3314 0.2463 0.0274 0.0752 0.082 0.3697 0.2848 0.1564 0.0528
Bud Site 0.2947 0.4633 0.0955 0.0256 0.0357 0.0353 0.2944 0.5139 0.1245 0.0607 0.0355 0.0195 0.2923 0.6233 0.098 0.0624 0.0545 0.0256
Cell Periphery 0.014 0.0083 0.0018 0.0023 0.0065 0.0095 0.0134 0.0122 0.0065 0.005 0.0178 0.0055 0.0135 0.0064 0.0092 0.0079 0.0316 0.0062
Cytoplasm 0.0699 0.1278 0.034 0.0419 0.0076 0.0663 0.0305 0.1064 0.0127 0.0959 0.0948 0.0527 0.058 0.062 0.0304 0.1213 0.0126 0.0298
Cytoplasmic Foci 0.0754 0.024 0.016 0.004 0.0199 0.0413 0.0942 0.0313 0.0116 0.0159 0.0187 0.0459 0.0778 0.0065 0.0093 0.0135 0.0641 0.0406
Eisosomes 0.0014 0.0008 0.0003 0.0006 0.0006 0.0004 0.0018 0.0009 0.0021 0.0017 0.0013 0.0003 0.0021 0.0002 0.0011 0.0009 0.0012 0.0004
Endoplasmic Reticulum 0.0052 0.0018 0.0011 0.0024 0.0003 0.0009 0.0045 0.0015 0.0009 0.0049 0.0047 0.0004 0.0037 0.0002 0.0008 0.0009 0.0007 0.0004
Endosome 0.0236 0.003 0.0027 0.0022 0.0013 0.0111 0.0175 0.0032 0.0043 0.0095 0.0019 0.0017 0.0345 0.0007 0.0037 0.0091 0.0082 0.0031
Golgi 0.0178 0.0038 0.0016 0.0054 0.0037 0.0072 0.0106 0.0016 0.0025 0.006 0.0046 0.0023 0.0323 0.0011 0.0015 0.0174 0.0239 0.0044
Lipid Particles 0.0152 0.0017 0.0016 0.0007 0.0008 0.0056 0.0091 0.0004 0.0019 0.0015 0.0015 0.0031 0.0148 0.0003 0.0056 0.0019 0.0168 0.0043
Mitochondria 0.0114 0.0028 0.0149 0.0105 0.006 0.0036 0.0067 0.0012 0.0038 0.0032 0.0025 0.0006 0.0076 0.0005 0.0009 0.0345 0.0521 0.0103
None 0.0318 0.0508 0.0285 0.0154 0.0097 0.0028 0.0259 0.0655 0.0337 0.1041 0.0072 0.0132 0.0079 0.0262 0.0185 0.0058 0.0031 0.0067
Nuclear Periphery 0.0159 0.0008 0.0011 0.0027 0.0001 0.0014 0.0064 0.0059 0.0008 0.0013 0.0004 0.0002 0.0242 0.0001 0.0001 0.0003 0.0004 0.0002
Nucleolus 0.0046 0.0019 0.0002 0.0007 0.0005 0.001 0.0063 0.001 0.0025 0.0009 0.0011 0.0004 0.0006 0.0017 0.0005 0.0006 0.0006 0.0023
Nucleus 0.0092 0.0045 0.0008 0.0031 0.0004 0.0011 0.003 0.002 0.0018 0.0019 0.0009 0.0004 0.0065 0.0021 0.0004 0.0013 0.0005 0.0007
Peroxisomes 0.0196 0.0115 0.0294 0.0066 0.0341 0.0241 0.0164 0.0029 0.009 0.0043 0.0323 0.0168 0.0219 0.0068 0.0058 0.0192 0.0356 0.0051
Punctate Nuclear 0.0203 0.0214 0.0009 0.0019 0.0004 0.0084 0.0212 0.0071 0.0025 0.0033 0.0009 0.0033 0.0065 0.0003 0.0007 0.0006 0.002 0.0024
Vacuole 0.0075 0.0041 0.001 0.0048 0.0018 0.0021 0.0028 0.0019 0.0021 0.0021 0.0028 0.0007 0.0021 0.0042 0.0009 0.0059 0.0013 0.002
Vacuole Periphery 0.0019 0.0005 0.0003 0.0009 0.0003 0.0004 0.0008 0.0009 0.0004 0.0004 0.0003 0.0001 0.0021 0.0003 0.0001 0.0007 0.0004 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.2621 8.9575 10.9477 13.6358 12.6096 17.673 10.6552 16.1809 11.1679 17.0472
Translational Efficiency 1.1037 0.9397 1.003 0.7614 0.9016 0.9303 0.9724 0.6689 1.3448 0.8867

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress
Localization
Cell Percentages actin (13%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Exo70

Exo70


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Exo70-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available