Standard name | |
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Human Ortholog | |
Description | Mitochondrial ribosomal protein of the large subunit |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 2 | – | 5 | 0 | 1 | 0 | – | 0 | 0 | 2 | 0 | 3 | 2 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 2 | 6 | 7 |
Bud | 1 | 2 | – | 2 | 1 | 4 | 2 | – | 2 | 5 | 8 | 4 | 1 | 5 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 |
Bud Neck | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Bud Site | 0 | 0 | – | 0 | 0 | 1 | 0 | – | 1 | 3 | 1 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 2 | – | 7 | 4 | 0 | 4 | – | 0 | 2 | 0 | 4 | 6 | 8 | 11 | 6 | 2 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3 | 3 | – | 4 | 17 | 6 | 4 | – | 3 | 5 | 2 | 1 | 6 | 14 | 21 | 22 | 7 | 5 | 1 | 0 | 0 | 0 | 0 | 2 |
Endoplasmic Reticulum | 3 | 5 | – | 2 | 2 | 1 | 2 | – | 3 | 2 | 0 | 0 | 3 | 2 | 6 | 5 | 4 | 1 | 0 | 1 | 0 | 0 | 1 | 6 |
Endosome | 3 | 0 | – | 10 | 25 | 0 | 20 | – | 1 | 7 | 7 | 3 | 10 | 14 | 15 | 13 | 7 | 3 | 6 | 6 | 3 | 7 | 18 | 18 |
Golgi | 3 | 1 | – | 26 | 41 | 0 | 8 | – | 2 | 4 | 1 | 1 | 31 | 41 | 34 | 12 | 5 | 8 | 5 | 7 | 1 | 6 | 17 | 27 |
Mitochondria | 94 | 86 | – | 111 | 121 | 118 | 153 | – | 160 | 191 | 193 | 138 | 130 | 257 | 287 | 11 | 6 | 2 | 99 | 77 | 25 | 96 | 164 | 183 |
Nucleus | 0 | 1 | – | 0 | 1 | 5 | 5 | – | 1 | 3 | 4 | 3 | 0 | 0 | 3 | 5 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 6 | 4 | – | 1 | 2 | 6 | 15 | – | 23 | 13 | 7 | 13 | 6 | 6 | 8 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 3 |
Nucleolus | 4 | 6 | – | 1 | 4 | 6 | 15 | – | 14 | 18 | 16 | 13 | 4 | 4 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 |
Peroxisomes | 2 | 0 | – | 3 | 0 | 0 | 1 | – | 3 | 0 | 1 | 3 | 5 | 11 | 10 | 7 | 0 | 3 | 6 | 2 | 4 | 12 | 24 | 25 |
SpindlePole | 1 | 0 | – | 3 | 9 | 1 | 14 | – | 2 | 7 | 6 | 7 | 1 | 5 | 3 | 6 | 6 | 2 | 0 | 0 | 0 | 1 | 5 | 1 |
Vac/Vac Membrane | 18 | 14 | – | 11 | 58 | 9 | 34 | – | 9 | 9 | 7 | 2 | 25 | 44 | 40 | 15 | 10 | 12 | 1 | 4 | 0 | 1 | 6 | 5 |
Unique Cell Count | 104 | 98 | 141 | 210 | 136 | 210 | 180 | 225 | 210 | 161 | 162 | 300 | 354 | 89 | 37 | 35 | 124 | 106 | 38 | 134 | 258 | 293 | ||
Labelled Cell Count | 140 | 126 | 186 | 285 | 158 | 277 | 224 | 269 | 255 | 193 | 231 | 413 | 442 | 106 | 48 | 41 | 124 | 106 | 38 | 134 | 258 | 293 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.5 | 6.3 | – | 7.0 | 8.1 | 4.9 | 6.9 | – | 5.6 | 6.1 | 6.1 | 6.2 | 7.3 | 6.9 | 6.9 | 8.2 | 8.5 | 8.3 | 6.5 | 7.0 | 7.1 |
Std Deviation (1e-4) | 1.1 | 1.2 | – | 1.3 | 2.3 | 1.4 | 1.6 | – | 1.1 | 1.4 | 1.3 | 1.2 | 1.6 | 1.5 | 1.5 | 2.6 | 2.1 | 2.3 | 1.6 | 1.8 | 2.0 |
Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.7568 | 3.689 | 3.2515 | 2.8901 | 2.6741 | 2.9657 | 4.8509 | 5.0258 | 4.8381 | 4.4432 | 4.5083 | 4.8587 | 6.0087 | 6.2612 | 5.756 | 6.0255 | 5.0176 | 5.6989 |
Actin | 0.0219 | 0.0058 | 0.0098 | 0.0506 | 0.0008 | 0.0013 | 0.0402 | 0.0052 | 0.0022 | 0.0005 | 0.0012 | 0.0176 | 0.0125 | 0.0032 | 0.0128 | 0.0001 | 0.0202 | 0.0005 |
Bud | 0.0004 | 0.0002 | 0.0003 | 0.0011 | 0.0001 | 0 | 0.0005 | 0.0011 | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0016 | 0.0002 | 0 | 0.0005 | 0 |
Bud Neck | 0.0009 | 0.0001 | 0.0017 | 0.0022 | 0 | 0 | 0.0042 | 0.0003 | 0.0003 | 0 | 0 | 0.0001 | 0.0003 | 0.0002 | 0.0006 | 0 | 0.0001 | 0 |
Bud Periphery | 0.0007 | 0.0004 | 0.0004 | 0.0019 | 0.0002 | 0.0001 | 0.0007 | 0.0014 | 0.0012 | 0.0003 | 0.0004 | 0.0002 | 0.0005 | 0.0042 | 0.0005 | 0 | 0.0021 | 0.0001 |
Bud Site | 0.0018 | 0.0006 | 0.0066 | 0.0033 | 0.0001 | 0 | 0.0089 | 0.0071 | 0.001 | 0 | 0.0002 | 0.0002 | 0.0009 | 0.0061 | 0.0009 | 0 | 0.0004 | 0 |
Cell Periphery | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0003 | 0.0007 | 0.0002 | 0 | 0.0003 | 0.0001 |
Cytoplasm | 0.0007 | 0.0002 | 0.0012 | 0.0024 | 0 | 0 | 0.0043 | 0.0004 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0045 | 0.0002 | 0.0002 | 0 | 0.0008 | 0 |
Cytoplasmic Foci | 0.0131 | 0.0077 | 0.0141 | 0.0243 | 0.0072 | 0.0008 | 0.0115 | 0.0078 | 0.0021 | 0.0019 | 0.0121 | 0.0021 | 0.0145 | 0.003 | 0.0059 | 0.0001 | 0.0022 | 0.0007 |
Eisosomes | 0.0003 | 0.0001 | 0.0002 | 0.0005 | 0.0001 | 0.0001 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0004 | 0.0002 | 0.0021 | 0 | 0.0003 | 0.0001 |
Endoplasmic Reticulum | 0.0005 | 0.0008 | 0.0007 | 0.002 | 0 | 0 | 0.0018 | 0.0002 | 0.0006 | 0 | 0 | 0.0001 | 0.0016 | 0.0001 | 0.0001 | 0 | 0.001 | 0.0001 |
Endosome | 0.0259 | 0.0322 | 0.0105 | 0.0455 | 0.0089 | 0.0033 | 0.0261 | 0.0153 | 0.0186 | 0.0014 | 0.0272 | 0.0094 | 0.0163 | 0.0077 | 0.0065 | 0.0004 | 0.0077 | 0.0048 |
Golgi | 0.0337 | 0.0614 | 0.0155 | 0.0287 | 0.0142 | 0.0091 | 0.0255 | 0.0321 | 0.0214 | 0.0029 | 0.0189 | 0.0305 | 0.01 | 0.0124 | 0.0154 | 0.0008 | 0.0082 | 0.0123 |
Lipid Particles | 0.0257 | 0.0047 | 0.0117 | 0.0138 | 0.006 | 0.0025 | 0.0572 | 0.0153 | 0.0059 | 0.0092 | 0.0076 | 0.0121 | 0.032 | 0.0096 | 0.012 | 0.0002 | 0.0054 | 0.0046 |
Mitochondria | 0.8431 | 0.8483 | 0.8955 | 0.785 | 0.9068 | 0.9672 | 0.746 | 0.8797 | 0.9141 | 0.9706 | 0.8722 | 0.9099 | 0.8389 | 0.9149 | 0.9183 | 0.9972 | 0.8506 | 0.9628 |
None | 0.0003 | 0.0001 | 0.0015 | 0.0021 | 0 | 0 | 0.0015 | 0.0032 | 0.0002 | 0 | 0 | 0.0001 | 0.0059 | 0.0001 | 0.0012 | 0 | 0.048 | 0 |
Nuclear Periphery | 0.002 | 0.0044 | 0.0008 | 0.0027 | 0 | 0 | 0.0117 | 0.0018 | 0.0042 | 0 | 0 | 0.0001 | 0.0086 | 0.0001 | 0.0001 | 0 | 0.0007 | 0.0001 |
Nucleolus | 0.0006 | 0.0001 | 0.0003 | 0.0004 | 0.0001 | 0 | 0.0005 | 0.0002 | 0.0003 | 0 | 0.0001 | 0 | 0.0008 | 0.0002 | 0.0001 | 0 | 0.0002 | 0 |
Nucleus | 0.0008 | 0.0004 | 0.0002 | 0.0008 | 0 | 0 | 0.008 | 0.0003 | 0.0005 | 0 | 0 | 0 | 0.0023 | 0.0002 | 0.0001 | 0 | 0.0002 | 0 |
Peroxisomes | 0.0208 | 0.024 | 0.0249 | 0.0257 | 0.0527 | 0.0136 | 0.0398 | 0.0147 | 0.0067 | 0.0124 | 0.0579 | 0.0144 | 0.0202 | 0.0243 | 0.0182 | 0.0007 | 0.0445 | 0.0114 |
Punctate Nuclear | 0.0005 | 0 | 0.001 | 0.0024 | 0 | 0 | 0.0018 | 0.0002 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0057 | 0 | 0.0001 | 0 | 0.0002 | 0 |
Vacuole | 0.0032 | 0.0036 | 0.0014 | 0.002 | 0.0011 | 0.0004 | 0.0048 | 0.004 | 0.0104 | 0.0001 | 0.0007 | 0.0006 | 0.0124 | 0.0051 | 0.0014 | 0.0001 | 0.0031 | 0.0004 |
Vacuole Periphery | 0.0029 | 0.0046 | 0.0015 | 0.0022 | 0.0014 | 0.0013 | 0.0042 | 0.0091 | 0.0089 | 0.0003 | 0.001 | 0.0019 | 0.0111 | 0.0056 | 0.0032 | 0.0003 | 0.0034 | 0.002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 14.7575 | 20.2958 | 15.0604 | 31.208 | 22.0773 | 27.2295 | 26.6774 | 27.7007 | 24.9427 | 41.281 |
Translational Efficiency | 2.6377 | 1.5543 | 2.009 | 1.081 | 1.4537 | 1.9089 | 1.7542 | 1.5468 | 1.9641 | 1.6564 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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