Standard name
Human Ortholog
Description Mitochondrial elongation factor involved in translational elongation

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0.18 0.13 0 0 0 0 0 0.05
Bud 0 0 0.05 0 0.05 0 0 0.06 0.05 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Bud Neck 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0 0 0 0 0 0
Cytoplasm 0.1 0.22 0.29 0.35 0.33 0.11 0.1 0.07 0.06 0.06 0.07 0.48 0.69 0.55 0.08 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0
Endosome 0 0.1 0.07 0 0 0 0 0 0 0 0 0 0.09 0.13 0.06 0.09 0.07 0.11 0.08 0.09 0.07 0.08
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.3 0.22 0 0 0 0 0 0
Mitochondria 0.84 0.63 0.4 0.62 0.49 0.8 0.8 0.89 0.9 0.93 0.84 0.54 0.19 0.32 0.16 0.16 0.68 0.68 0.72 0.59 0.57 0.54
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.05 0 0.05 0 0 0 0 0 0 0 0.08 0.06 0 0 0.07 0 0 0.05 0.06 0.06
SpindlePole 0 0 0.13 0.08 0.09 0.06 0.1 0.06 0.06 0 0.09 0 0 0 0.08 0.08 0 0 0 0 0 0
Vac/Vac Membrane 0.07 0.08 0 0 0.05 0.05 0 0 0 0 0 0 0.07 0.07 0.15 0.21 0 0 0 0.07 0.07 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 7 0 3 0 4 0 1 0 1 4 0 0 70 33 6 8 4 8 11 18
Bud 7 5 8 1 7 4 12 17 13 11 10 5 4 8 20 9 0 1 1 3 7 9
Bud Neck 0 1 7 0 1 1 0 1 1 0 2 0 0 0 0 1 1 2 1 6 11 15
Bud Site 1 1 0 0 0 1 5 6 6 3 5 0 0 0 2 0
Cell Periphery 2 11 5 1 2 2 2 2 1 0 4 1 4 1 28 15 1 0 1 1 2 1
Cytoplasm 21 67 45 18 44 19 28 18 17 17 18 126 193 211 30 27 5 11 5 11 10 8
Endoplasmic Reticulum 5 8 3 1 0 1 8 0 2 5 1 5 4 8 26 11 2 2 2 1 3 6
Endosome 6 30 10 1 6 3 7 1 1 0 0 8 26 49 23 23 15 44 22 24 21 27
Golgi 0 2 1 0 1 1 0 0 0 0 0 0 0 0 119 56 8 15 5 5 3 8
Mitochondria 173 193 61 32 65 143 225 235 235 248 219 142 52 121 63 39 161 274 195 152 181 191
Nucleus 2 2 1 1 1 7 12 9 10 7 8 0 1 5 16 6 0 1 0 1 1 1
Nuclear Periphery 1 2 0 0 0 3 5 2 1 4 4 1 1 0 9 7 1 2 0 0 2 2
Nucleolus 3 1 0 0 0 3 5 1 2 2 1 0 0 1 5 3 0 0 1 0 2 1
Peroxisomes 3 13 7 2 7 1 6 3 0 0 6 2 23 24 12 5 17 12 9 12 19 21
SpindlePole 5 13 20 4 12 11 28 15 15 10 24 4 5 8 33 20 3 5 4 6 6 8
Vac/Vac Membrane 14 26 5 2 7 9 12 6 11 3 7 11 19 28 59 53 4 15 8 17 20 25
Unique Cell Count 207 307 153 52 134 179 283 263 262 267 262 262 278 381 391 249 236 404 273 260 318 355
Labelled Cell Count 243 376 180 63 156 209 359 316 316 310 310 309 332 464 515 308 236 404 273 260 318 355


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.7 4.9 5.1 4.9 5.9 4.8 4.8 4.1 4.0 3.7 4.5 4.2 4.4 4.7 9.1 9.0 4.3 4.3 4.9
Std Deviation (1e-4) 0.8 1.1 1.0 2.2 2.0 1.5 1.6 1.1 1.3 0.9 1.4 0.8 1.3 1.5 1.6 2.0 1.7 1.5 1.9
Intensity Change (Log2) -0.04 0.22 -0.08 -0.07 -0.31 -0.34 -0.46 -0.18 -0.28 -0.22 -0.11 0.84 0.84 -0.23 -0.23 -0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 4.0 2.8
Bud 0 0 0 0 0 0 0 0 0 0 0 -0.1 -0.8
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 1.7 1.2
Cytoplasm 0.7 0.6 -4.3 -5.2 -6.2 -6.3 -6.4 -6.2 3.7 8.0 5.4 -6.6 -4.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 2.2 1.3
Endosome 0 -0.8 0 0 0 0 0 0 0 0 0 -0.3 1.0
Golgi 0 0 0 0 0 0 0 0 0 0 0 7.5 6.1
Mitochondria 2.7 1.5 0 0 0 0 0 0 0 0 0 -5.9 -5.5
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0.3 0 0 0 0 0 0 0 0 0 -0.9 0
SpindlePole -1.0 -1.1 0 0 0 0 0 0 0 0 0 -1.6 -1.6
Vacuole 0 0.8 0.8 0.5 0 0.5 0 0 0.5 1.5 1.8 3.8 5.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.9085 2.9897 2.8286 2.7744 3.7319 3.4433 1.0321 1.5663 1.6772 1.5412 1.1192 1.4676 0.3065 0.3593 0.1196 0.8007 0.1187 0.4157
Actin 0.079 0.0239 0.0256 0.0047 0.0774 0.0194 0.0304 0.0253 0.0298 0.0457 0.0065 0.019 0.0391 0.0154 0.0108 0.0385 0.0063 0.0105
Bud 0.0019 0.0033 0.0041 0.0032 0.0009 0.0008 0.0013 0.0028 0.0034 0.011 0.0088 0.001 0.0017 0.0026 0.0059 0.0083 0.0018 0.0014
Bud Neck 0.0058 0.0035 0.0043 0.0017 0.0007 0.0096 0.0011 0.0015 0.0013 0.0008 0.0035 0.0083 0.0015 0.001 0.0021 0.0031 0.0006 0.0055
Bud Periphery 0.0053 0.0091 0.0151 0.0119 0.0021 0.0021 0.0028 0.0042 0.0073 0.0162 0.0205 0.0021 0.0046 0.0048 0.0057 0.0182 0.0027 0.0024
Bud Site 0.0085 0.0311 0.0349 0.0026 0.0024 0.0034 0.0046 0.0236 0.0059 0.0103 0.0108 0.0019 0.0063 0.0406 0.0103 0.0102 0.0028 0.0011
Cell Periphery 0.0029 0.0051 0.0115 0.0019 0.0007 0.0014 0.0014 0.0007 0.0021 0.0009 0.0023 0.0004 0.0012 0.0018 0.0015 0.0013 0.0004 0.001
Cytoplasm 0.0247 0.0412 0.031 0.0229 0.006 0.0346 0.0146 0.025 0.0411 0.0227 0.0804 0.0199 0.0307 0.057 0.0473 0.0953 0.0371 0.0712
Cytoplasmic Foci 0.0854 0.0921 0.0684 0.0536 0.0244 0.1038 0.0602 0.1037 0.0528 0.0616 0.054 0.0623 0.1304 0.106 0.0779 0.1436 0.1354 0.1062
Eisosomes 0.0021 0.002 0.0021 0.0007 0.0022 0.0008 0.0008 0.0004 0.0009 0.0004 0.0004 0.0003 0.0022 0.0011 0.0005 0.0002 0.0004 0.0004
Endoplasmic Reticulum 0.011 0.0035 0.0087 0.0011 0.002 0.0036 0.0103 0.002 0.0056 0.0028 0.0058 0.0035 0.0099 0.0042 0.0043 0.0018 0.0069 0.0029
Endosome 0.1379 0.1151 0.0929 0.0786 0.0827 0.1064 0.1671 0.1401 0.1546 0.1335 0.1443 0.1358 0.1776 0.1774 0.1298 0.1231 0.1887 0.1173
Golgi 0.0638 0.0636 0.0498 0.0411 0.0442 0.0523 0.0899 0.1338 0.0886 0.0843 0.0551 0.1079 0.0725 0.0859 0.0477 0.051 0.0751 0.0488
Lipid Particles 0.093 0.0276 0.0309 0.02 0.0155 0.0358 0.0558 0.0207 0.0141 0.0278 0.0627 0.0269 0.0605 0.0231 0.0273 0.0051 0.0313 0.0215
Mitochondria 0.223 0.3468 0.4635 0.6424 0.5824 0.4716 0.4171 0.4425 0.5142 0.5295 0.4773 0.5642 0.2754 0.3176 0.5053 0.408 0.3126 0.4618
None 0.1323 0.1698 0.0926 0.073 0.1282 0.0876 0.0559 0.0058 0.0238 0.0137 0.004 0.0093 0.0929 0.1059 0.0655 0.033 0.1208 0.0735
Nuclear Periphery 0.0274 0.0007 0.0029 0.0003 0.0015 0.0014 0.0125 0.0007 0.0025 0.0023 0.0024 0.0012 0.0139 0.0036 0.0053 0.0006 0.0055 0.0031
Nucleolus 0.0026 0.001 0.0013 0.0027 0.0002 0.002 0.0024 0.0005 0.0009 0.001 0.0007 0.0003 0.0031 0.0012 0.0012 0.0006 0.0007 0.0008
Nucleus 0.0036 0.0009 0.0015 0.0014 0.0004 0.0015 0.003 0.0014 0.0013 0.0013 0.0016 0.0009 0.0052 0.0015 0.0016 0.001 0.0012 0.0019
Peroxisomes 0.0479 0.0388 0.0334 0.0245 0.0206 0.0446 0.0354 0.0506 0.0236 0.0137 0.0229 0.0253 0.048 0.0293 0.0252 0.032 0.058 0.0559
Punctate Nuclear 0.0179 0.0011 0.0007 0.0004 0.0008 0.0017 0.003 0.0007 0.0012 0.0035 0.0004 0.0007 0.0058 0.0017 0.0012 0.0011 0.0029 0.0029
Vacuole 0.0159 0.0142 0.0151 0.0081 0.0025 0.0097 0.0198 0.0098 0.0172 0.0111 0.0234 0.0052 0.0106 0.0117 0.0171 0.0172 0.0051 0.0061
Vacuole Periphery 0.0081 0.0054 0.0099 0.0033 0.0021 0.006 0.0107 0.004 0.0077 0.006 0.0123 0.0038 0.0067 0.0065 0.0067 0.0068 0.0038 0.004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.3825 8.4203 6.8433 8.3214 3.4893 2.6897 7.8043 7.5248 8.1414 4.8885
Translational Efficiency 0.5583 0.5473 0.5126 0.5569 0.6861 0.9354 0.6419 0.5597 0.6002 0.6005

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Mitochondrial elongation factor involved in translational elongation
Localization
Cell Percentages mitochondrion (41%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Mef2

Mef2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mef2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available