Standard name | |
---|---|
Human Ortholog | |
Description | Mitochondrial elongation factor involved in translational elongation |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 7 | 0 | 3 | 0 | 4 | – | 0 | 1 | 0 | 1 | 4 | 0 | 0 | 70 | 33 | – | 6 | 8 | 4 | 8 | 11 | 18 |
Bud | 7 | 5 | 8 | 1 | 7 | 4 | 12 | – | 17 | 13 | 11 | 10 | 5 | 4 | 8 | 20 | 9 | – | 0 | 1 | 1 | 3 | 7 | 9 |
Bud Neck | 0 | 1 | 7 | 0 | 1 | 1 | 0 | – | 1 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | – | 1 | 2 | 1 | 6 | 11 | 15 |
Bud Site | 1 | 1 | 0 | 0 | 0 | 1 | 5 | – | 6 | 6 | 3 | 5 | 0 | 0 | 0 | 2 | 0 | – | – | – | – | – | – | – |
Cell Periphery | 2 | 11 | 5 | 1 | 2 | 2 | 2 | – | 2 | 1 | 0 | 4 | 1 | 4 | 1 | 28 | 15 | – | 1 | 0 | 1 | 1 | 2 | 1 |
Cytoplasm | 21 | 67 | 45 | 18 | 44 | 19 | 28 | – | 18 | 17 | 17 | 18 | 126 | 193 | 211 | 30 | 27 | – | 5 | 11 | 5 | 11 | 10 | 8 |
Endoplasmic Reticulum | 5 | 8 | 3 | 1 | 0 | 1 | 8 | – | 0 | 2 | 5 | 1 | 5 | 4 | 8 | 26 | 11 | – | 2 | 2 | 2 | 1 | 3 | 6 |
Endosome | 6 | 30 | 10 | 1 | 6 | 3 | 7 | – | 1 | 1 | 0 | 0 | 8 | 26 | 49 | 23 | 23 | – | 15 | 44 | 22 | 24 | 21 | 27 |
Golgi | 0 | 2 | 1 | 0 | 1 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 119 | 56 | – | 8 | 15 | 5 | 5 | 3 | 8 |
Mitochondria | 173 | 193 | 61 | 32 | 65 | 143 | 225 | – | 235 | 235 | 248 | 219 | 142 | 52 | 121 | 63 | 39 | – | 161 | 274 | 195 | 152 | 181 | 191 |
Nucleus | 2 | 2 | 1 | 1 | 1 | 7 | 12 | – | 9 | 10 | 7 | 8 | 0 | 1 | 5 | 16 | 6 | – | 0 | 1 | 0 | 1 | 1 | 1 |
Nuclear Periphery | 1 | 2 | 0 | 0 | 0 | 3 | 5 | – | 2 | 1 | 4 | 4 | 1 | 1 | 0 | 9 | 7 | – | 1 | 2 | 0 | 0 | 2 | 2 |
Nucleolus | 3 | 1 | 0 | 0 | 0 | 3 | 5 | – | 1 | 2 | 2 | 1 | 0 | 0 | 1 | 5 | 3 | – | 0 | 0 | 1 | 0 | 2 | 1 |
Peroxisomes | 3 | 13 | 7 | 2 | 7 | 1 | 6 | – | 3 | 0 | 0 | 6 | 2 | 23 | 24 | 12 | 5 | – | 17 | 12 | 9 | 12 | 19 | 21 |
SpindlePole | 5 | 13 | 20 | 4 | 12 | 11 | 28 | – | 15 | 15 | 10 | 24 | 4 | 5 | 8 | 33 | 20 | – | 3 | 5 | 4 | 6 | 6 | 8 |
Vac/Vac Membrane | 14 | 26 | 5 | 2 | 7 | 9 | 12 | – | 6 | 11 | 3 | 7 | 11 | 19 | 28 | 59 | 53 | – | 4 | 15 | 8 | 17 | 20 | 25 |
Unique Cell Count | 207 | 307 | 153 | 52 | 134 | 179 | 283 | 263 | 262 | 267 | 262 | 262 | 278 | 381 | 391 | 249 | 236 | 404 | 273 | 260 | 318 | 355 | ||
Labelled Cell Count | 243 | 376 | 180 | 63 | 156 | 209 | 359 | 316 | 316 | 310 | 310 | 309 | 332 | 464 | 515 | 308 | 236 | 404 | 273 | 260 | 318 | 355 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.7 | 4.9 | 5.1 | 4.9 | 5.9 | 4.8 | 4.8 | – | 4.1 | 4.0 | 3.7 | 4.5 | 4.2 | 4.4 | 4.7 | 9.1 | 9.0 | – | 4.3 | 4.3 | 4.9 |
Std Deviation (1e-4) | 0.8 | 1.1 | 1.0 | 2.2 | 2.0 | 1.5 | 1.6 | – | 1.1 | 1.3 | 0.9 | 1.4 | 0.8 | 1.3 | 1.5 | 1.6 | 2.0 | – | 1.7 | 1.5 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.04 | 0.22 | -0.08 | -0.07 | – | -0.31 | -0.34 | -0.46 | -0.18 | -0.28 | -0.22 | -0.11 | 0.84 | 0.84 | – | -0.23 | -0.23 | -0.05 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.0 | 2.8 | – |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.1 | -0.8 | – |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.7 | 1.2 | – |
Cytoplasm | 0.7 | 0.6 | -4.3 | -5.2 | – | -6.2 | -6.3 | -6.4 | -6.2 | 3.7 | 8.0 | 5.4 | -6.6 | -4.7 | – |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.2 | 1.3 | – |
Endosome | 0 | -0.8 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.3 | 1.0 | – |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 7.5 | 6.1 | – |
Mitochondria | 2.7 | 1.5 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -5.9 | -5.5 | – |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Peroxisomes | 0 | 0.3 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.9 | 0 | – |
SpindlePole | -1.0 | -1.1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.6 | -1.6 | – |
Vacuole | 0 | 0.8 | 0.8 | 0.5 | – | 0 | 0.5 | 0 | 0 | 0.5 | 1.5 | 1.8 | 3.8 | 5.0 | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.9085 | 2.9897 | 2.8286 | 2.7744 | 3.7319 | 3.4433 | 1.0321 | 1.5663 | 1.6772 | 1.5412 | 1.1192 | 1.4676 | 0.3065 | 0.3593 | 0.1196 | 0.8007 | 0.1187 | 0.4157 |
Actin | 0.079 | 0.0239 | 0.0256 | 0.0047 | 0.0774 | 0.0194 | 0.0304 | 0.0253 | 0.0298 | 0.0457 | 0.0065 | 0.019 | 0.0391 | 0.0154 | 0.0108 | 0.0385 | 0.0063 | 0.0105 |
Bud | 0.0019 | 0.0033 | 0.0041 | 0.0032 | 0.0009 | 0.0008 | 0.0013 | 0.0028 | 0.0034 | 0.011 | 0.0088 | 0.001 | 0.0017 | 0.0026 | 0.0059 | 0.0083 | 0.0018 | 0.0014 |
Bud Neck | 0.0058 | 0.0035 | 0.0043 | 0.0017 | 0.0007 | 0.0096 | 0.0011 | 0.0015 | 0.0013 | 0.0008 | 0.0035 | 0.0083 | 0.0015 | 0.001 | 0.0021 | 0.0031 | 0.0006 | 0.0055 |
Bud Periphery | 0.0053 | 0.0091 | 0.0151 | 0.0119 | 0.0021 | 0.0021 | 0.0028 | 0.0042 | 0.0073 | 0.0162 | 0.0205 | 0.0021 | 0.0046 | 0.0048 | 0.0057 | 0.0182 | 0.0027 | 0.0024 |
Bud Site | 0.0085 | 0.0311 | 0.0349 | 0.0026 | 0.0024 | 0.0034 | 0.0046 | 0.0236 | 0.0059 | 0.0103 | 0.0108 | 0.0019 | 0.0063 | 0.0406 | 0.0103 | 0.0102 | 0.0028 | 0.0011 |
Cell Periphery | 0.0029 | 0.0051 | 0.0115 | 0.0019 | 0.0007 | 0.0014 | 0.0014 | 0.0007 | 0.0021 | 0.0009 | 0.0023 | 0.0004 | 0.0012 | 0.0018 | 0.0015 | 0.0013 | 0.0004 | 0.001 |
Cytoplasm | 0.0247 | 0.0412 | 0.031 | 0.0229 | 0.006 | 0.0346 | 0.0146 | 0.025 | 0.0411 | 0.0227 | 0.0804 | 0.0199 | 0.0307 | 0.057 | 0.0473 | 0.0953 | 0.0371 | 0.0712 |
Cytoplasmic Foci | 0.0854 | 0.0921 | 0.0684 | 0.0536 | 0.0244 | 0.1038 | 0.0602 | 0.1037 | 0.0528 | 0.0616 | 0.054 | 0.0623 | 0.1304 | 0.106 | 0.0779 | 0.1436 | 0.1354 | 0.1062 |
Eisosomes | 0.0021 | 0.002 | 0.0021 | 0.0007 | 0.0022 | 0.0008 | 0.0008 | 0.0004 | 0.0009 | 0.0004 | 0.0004 | 0.0003 | 0.0022 | 0.0011 | 0.0005 | 0.0002 | 0.0004 | 0.0004 |
Endoplasmic Reticulum | 0.011 | 0.0035 | 0.0087 | 0.0011 | 0.002 | 0.0036 | 0.0103 | 0.002 | 0.0056 | 0.0028 | 0.0058 | 0.0035 | 0.0099 | 0.0042 | 0.0043 | 0.0018 | 0.0069 | 0.0029 |
Endosome | 0.1379 | 0.1151 | 0.0929 | 0.0786 | 0.0827 | 0.1064 | 0.1671 | 0.1401 | 0.1546 | 0.1335 | 0.1443 | 0.1358 | 0.1776 | 0.1774 | 0.1298 | 0.1231 | 0.1887 | 0.1173 |
Golgi | 0.0638 | 0.0636 | 0.0498 | 0.0411 | 0.0442 | 0.0523 | 0.0899 | 0.1338 | 0.0886 | 0.0843 | 0.0551 | 0.1079 | 0.0725 | 0.0859 | 0.0477 | 0.051 | 0.0751 | 0.0488 |
Lipid Particles | 0.093 | 0.0276 | 0.0309 | 0.02 | 0.0155 | 0.0358 | 0.0558 | 0.0207 | 0.0141 | 0.0278 | 0.0627 | 0.0269 | 0.0605 | 0.0231 | 0.0273 | 0.0051 | 0.0313 | 0.0215 |
Mitochondria | 0.223 | 0.3468 | 0.4635 | 0.6424 | 0.5824 | 0.4716 | 0.4171 | 0.4425 | 0.5142 | 0.5295 | 0.4773 | 0.5642 | 0.2754 | 0.3176 | 0.5053 | 0.408 | 0.3126 | 0.4618 |
None | 0.1323 | 0.1698 | 0.0926 | 0.073 | 0.1282 | 0.0876 | 0.0559 | 0.0058 | 0.0238 | 0.0137 | 0.004 | 0.0093 | 0.0929 | 0.1059 | 0.0655 | 0.033 | 0.1208 | 0.0735 |
Nuclear Periphery | 0.0274 | 0.0007 | 0.0029 | 0.0003 | 0.0015 | 0.0014 | 0.0125 | 0.0007 | 0.0025 | 0.0023 | 0.0024 | 0.0012 | 0.0139 | 0.0036 | 0.0053 | 0.0006 | 0.0055 | 0.0031 |
Nucleolus | 0.0026 | 0.001 | 0.0013 | 0.0027 | 0.0002 | 0.002 | 0.0024 | 0.0005 | 0.0009 | 0.001 | 0.0007 | 0.0003 | 0.0031 | 0.0012 | 0.0012 | 0.0006 | 0.0007 | 0.0008 |
Nucleus | 0.0036 | 0.0009 | 0.0015 | 0.0014 | 0.0004 | 0.0015 | 0.003 | 0.0014 | 0.0013 | 0.0013 | 0.0016 | 0.0009 | 0.0052 | 0.0015 | 0.0016 | 0.001 | 0.0012 | 0.0019 |
Peroxisomes | 0.0479 | 0.0388 | 0.0334 | 0.0245 | 0.0206 | 0.0446 | 0.0354 | 0.0506 | 0.0236 | 0.0137 | 0.0229 | 0.0253 | 0.048 | 0.0293 | 0.0252 | 0.032 | 0.058 | 0.0559 |
Punctate Nuclear | 0.0179 | 0.0011 | 0.0007 | 0.0004 | 0.0008 | 0.0017 | 0.003 | 0.0007 | 0.0012 | 0.0035 | 0.0004 | 0.0007 | 0.0058 | 0.0017 | 0.0012 | 0.0011 | 0.0029 | 0.0029 |
Vacuole | 0.0159 | 0.0142 | 0.0151 | 0.0081 | 0.0025 | 0.0097 | 0.0198 | 0.0098 | 0.0172 | 0.0111 | 0.0234 | 0.0052 | 0.0106 | 0.0117 | 0.0171 | 0.0172 | 0.0051 | 0.0061 |
Vacuole Periphery | 0.0081 | 0.0054 | 0.0099 | 0.0033 | 0.0021 | 0.006 | 0.0107 | 0.004 | 0.0077 | 0.006 | 0.0123 | 0.0038 | 0.0067 | 0.0065 | 0.0067 | 0.0068 | 0.0038 | 0.004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 5.3825 | 8.4203 | 6.8433 | 8.3214 | 3.4893 | 2.6897 | 7.8043 | 7.5248 | 8.1414 | 4.8885 |
Translational Efficiency | 0.5583 | 0.5473 | 0.5126 | 0.5569 | 0.6861 | 0.9354 | 0.6419 | 0.5597 | 0.6002 | 0.6005 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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