Standard name
Human Ortholog
Description Subunit of the import motor (PAM complex); the PAM complex is a component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); forms a 1:1 subcomplex with Pam18p and inhibits its cochaperone activity; contains a J-like domain
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.13 0.17 0.14 0 0 0 0 0.05 0.06
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.08 0.07 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 – 0.08 0 0 0 0 0 0 0 0.08 0.07 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0.06 0 0 0.05 0 – 0 0 0 0 0 0 0 0.08 0.46 0.34 0.42 0 0.08 0.06 0.11 0.15 0.14
Mitochondria 0.92 0.86 0.96 0.98 0.93 0.9 – 0.8 0.9 0.88 0.91 0.91 0.87 0.9 0.9 0.08 0.12 0.07 0.87 0.79 0.82 0.71 0.63 0.62
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0.05 0 0 0.1 – 0.05 0.1 0.06 0.13 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.13 0.1 0.08 0.09 0.09 0.18 – 0.17 0.17 0.13 0.16 0.14 0 0.08 0.05 0.1 0.1 0.17 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.1 0.11 0.06 0 0.05 0 – 0 0.05 0 0 0.05 0.16 0.09 0.09 0.12 0.22 0.15 0 0.07 0.05 0.11 0.07 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 0 2 1 1 0 – 2 0 0 0 2 0 2 5 7 10 14 2 1 0 1 16 24
Bud 0 0 1 2 8 5 – 13 7 15 7 15 2 3 1 2 1 4 0 1 0 0 2 4
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 1 2 0 1 – 7 5 2 2 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 2 3 0 1 0 – 4 1 3 4 6 1 0 3 4 4 3 0 0 0 0 0 0
Cytoplasm 0 1 2 0 5 7 – 34 8 23 13 13 1 2 7 4 4 9 0 0 0 0 0 0
Endoplasmic Reticulum 2 1 0 0 0 1 – 0 1 3 0 2 0 1 1 2 1 3 0 0 0 0 1 1
Endosome 0 0 1 0 1 0 – 1 1 3 3 1 0 0 1 0 0 2 2 0 0 2 6 8
Golgi 10 6 2 1 19 1 – 0 0 1 1 2 4 13 37 24 20 43 17 8 6 12 48 57
Mitochondria 365 85 102 218 346 246 – 357 359 492 275 552 167 328 410 4 7 7 348 80 86 80 206 250
Nucleus 1 0 0 0 0 1 – 11 2 11 2 4 1 0 0 1 0 2 0 0 0 1 1 1
Nuclear Periphery 9 2 5 5 8 28 – 22 41 36 40 51 6 7 7 2 0 1 3 0 0 0 1 3
Nucleolus 50 10 8 21 35 49 – 78 67 72 49 85 7 28 22 5 6 17 4 0 0 1 6 6
Peroxisomes 0 0 0 2 0 1 – 5 1 3 0 3 3 5 6 2 1 5 1 0 3 1 13 11
SpindlePole 1 0 0 1 0 0 – 2 3 1 2 7 0 0 0 0 0 2 0 0 0 0 1 2
Vac/Vac Membrane 38 11 6 4 18 5 – 16 18 21 5 30 30 33 39 6 13 15 13 6 5 12 21 25
Unique Cell Count 395 99 106 222 372 273 446 399 561 302 609 192 363 456 52 59 103 398 102 106 114 329 402
Labelled Cell Count 480 118 133 257 442 345 552 514 686 403 775 222 422 539 63 67 127 398 102 106 114 329 402


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 19.2 23.6 10.8 13.9 14.9 12.1 – 10.2 9.8 9.8 9.1 9.1 19.0 18.9 18.4 25.7 28.0 22.8 20.9 20.5 21.1
Std Deviation (1e-4) 3.9 5.5 2.1 3.0 3.1 3.7 – 3.6 3.0 2.7 2.9 2.5 4.6 4.2 4.2 8.0 9.4 8.1 9.8 5.1 5.6
Intensity Change (Log2) – – – 0.37 0.46 0.16 – -0.08 -0.14 -0.15 -0.25 -0.24 0.81 0.81 0.77 1.25 1.37 1.08 0.95 0.92 0.97


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 3.2
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 – 2.2 0 0 0 0 0 0 0 0 0 2.2
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 2.3 7.1 5.8 7.0
Mitochondria 1.1 -1.2 -2.0 – -4.0 -2.0 -2.6 -1.7 -1.9 -2.6 -1.9 -2.1 -11.1 -11.0 -12.9
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 1.7 – 0.1 1.8 0.7 2.4 1.3 0 0 0 0 0 0
Nucleolus 0.6 0.6 2.5 – 2.5 2.4 1.5 2.2 1.8 -1.5 0.1 -1.1 0 0.6 2.0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 -0.3 0 – 0 -0.5 0 0 -0.3 2.5 1.1 1.0 0 3.2 2.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 13.3382 14.1558 13.3844 13.9158 10.4468 13.2667 14.661 14.6739 13.9518 13.8045 13.6804 14.0177 17.7806 17.2387 17.7349 17.227 15.0113 17.2529
Actin 0.012 0.0058 0.0048 0.0257 0.0018 0.0087 0.0243 0.0037 0.0041 0.0092 0.002 0.0037 0.0133 0.0116 0.0051 0.0006 0.0172 0.0046
Bud 0.0027 0.0059 0.001 0.009 0.0001 0.0004 0.0005 0.0044 0.0013 0.0002 0.0001 0.0003 0.0007 0.0017 0.0015 0.0007 0.0003 0.0006
Bud Neck 0.0004 0.0007 0.0007 0.0007 0.0001 0.0019 0.0006 0.0019 0.0004 0.0005 0.0003 0.0006 0.0008 0.0006 0.0004 0.0008 0.0005 0.0091
Bud Periphery 0.0022 0.0106 0.0018 0.0151 0.0003 0.0005 0.0008 0.0075 0.0012 0.0005 0.0001 0.0006 0.0011 0.0025 0.0018 0.0012 0.0003 0.0013
Bud Site 0.0038 0.0301 0.0047 0.0045 0.0001 0.0008 0.0022 0.0239 0.0048 0.0004 0.0001 0.0004 0.0044 0.0058 0.0053 0.0021 0.0007 0.0006
Cell Periphery 0.0007 0.0026 0.0004 0.0008 0.0002 0.0003 0.0009 0.0035 0.0013 0.0004 0.0002 0.0005 0.0009 0.0016 0.0006 0.0007 0.0004 0.0003
Cytoplasm 0.0034 0.001 0.0005 0.0064 0.0001 0.0004 0.0012 0.0017 0.001 0.0001 0 0.0001 0.0036 0.0081 0.0003 0 0.0001 0.0028
Cytoplasmic Foci 0.0167 0.0071 0.0125 0.0067 0.0043 0.0082 0.0094 0.0127 0.0088 0.0005 0.002 0.0056 0.0124 0.0075 0.0032 0.0037 0.0075 0.0036
Eisosomes 0.0003 0.0002 0.0003 0.0002 0.0001 0.0002 0.0005 0.0003 0.0003 0.0005 0.0002 0.0002 0.0004 0.0002 0.0001 0.0001 0.0002 0.0002
Endoplasmic Reticulum 0.0014 0.0003 0.0005 0.0033 0.0001 0.0005 0.001 0.0005 0.0002 0.0004 0 0.0001 0.0007 0.0001 0.0001 0 0 0.0014
Endosome 0.013 0.0072 0.0099 0.0146 0.006 0.0216 0.0216 0.0264 0.0078 0.0033 0.0064 0.0097 0.0147 0.0113 0.0045 0.001 0.0018 0.0062
Golgi 0.0069 0.0173 0.0113 0.0041 0.0055 0.0302 0.0144 0.0188 0.0135 0.0069 0.0266 0.0263 0.0116 0.0148 0.012 0.0013 0.0122 0.0159
Lipid Particles 0.0296 0.0081 0.0126 0.0022 0.0707 0.0133 0.0216 0.0107 0.0125 0.0033 0.0262 0.0131 0.0241 0.0224 0.0115 0.0111 0.0043 0.0048
Mitochondria 0.8686 0.8728 0.9044 0.8945 0.8925 0.8941 0.8114 0.8008 0.8786 0.9589 0.9213 0.9223 0.8409 0.8175 0.9173 0.9527 0.9064 0.9222
None 0.0026 0.0003 0.0004 0.0017 0.0002 0.0002 0.0015 0.0002 0.0003 0.0007 0 0 0.0028 0.0001 0.0009 0 0.0002 0.0001
Nuclear Periphery 0.0061 0.0002 0.0008 0.002 0.0008 0.0005 0.0043 0.0011 0.001 0.002 0.0001 0 0.0013 0.0002 0.0001 0 0 0.0008
Nucleolus 0.0008 0.0032 0.0014 0.0002 0.0003 0.0002 0.0136 0.0104 0.0123 0.0005 0.0005 0.0061 0.0074 0.0117 0.005 0.0129 0.035 0.0009
Nucleus 0.0014 0.0008 0.0003 0.0007 0.0001 0.0002 0.0019 0.0026 0.0024 0.0006 0.0001 0.0004 0.0027 0.0013 0.0008 0.0007 0.0017 0.0014
Peroxisomes 0.0122 0.0069 0.0077 0.0017 0.0099 0.0057 0.0183 0.014 0.0067 0.0021 0.0029 0.0043 0.0161 0.02 0.008 0.006 0.0091 0.0088
Punctate Nuclear 0.0031 0.0007 0.0024 0.002 0.0007 0.0011 0.0021 0.0002 0.0034 0.0003 0 0 0.0016 0.0001 0.0008 0.0001 0.0003 0.0009
Vacuole 0.0053 0.0087 0.0057 0.0015 0.0017 0.0033 0.0184 0.0283 0.0246 0.0034 0.0013 0.0024 0.019 0.0267 0.0111 0.0027 0.0009 0.0036
Vacuole Periphery 0.0067 0.0095 0.0159 0.0024 0.0043 0.0078 0.0292 0.0263 0.0135 0.0054 0.0096 0.0033 0.0195 0.0343 0.0096 0.0014 0.0011 0.01

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 30.6501 41.647 47.1691 47.4728 30.2018 41.6611 38.2308 55.346 60.9035 57.4892
Translational Efficiency 2.9933 2.1175 1.7319 2.2742 2.8046 2.4941 3.2196 2.2186 2.0505 2.03

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of the import motor (PAM complex); the PAM complex is a component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); forms a 1:1 subcomplex with Pam18p and inhibits its cochaperone activity; contains a J-like domain
Localization
Cell Percentages mitochondrion (98%)
Cell Cycle Regulation No
Subcompartmental Group mito-3

Pam16

Pam16


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pam16-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available