Standard name
Human Ortholog
Description Peripheral protein of cytosolic face of mitochondrial outer membrane; required for mitochondrial fission; interacts with Fis1p and with the self-assembled oligomeric form of the dynamin-related GTPase Dnm1p; contains WD repeats; MDV1 has a paralog, CAF4, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – 0.09 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0.05 0 0 0 – 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.05 0.06 0.09 0 0 0 0 0 0 0 – 0.12 0.1 – 0.19 0.33 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – 0.05 0.06 0 0 0 0 0 0
Endosome 0.09 0 0.07 0.05 0.1 0 0 0 0 0 0 0 – 0.17 0.16 – 0.12 0.18 0.09 0.09 0.1 0.06 0.09 0.15
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – 0 0.08 0 0 0 0 0 0
Mitochondria 0.77 0.89 0.86 0.85 0.67 0.79 0.75 0.78 0.88 0.91 0.9 0.88 – 0.46 0.31 – 0.14 0.19 0.75 0.69 0.7 0.66 0.64 0.52
Nucleus 0 0 0 0 0 0.1 0.11 0.11 0 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0.06 0.08 0.07 0.09 0.13 0.06 0.12 0.12 0.1 0.1 – 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0.06 0.05 0 0.06 0.11 0.17 0.18 0.19 0.18 0.18 0.19 – 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0.05 – 0.12 0 0 0 0.05 0.05 0.05 0.09
SpindlePole 0 0 0 0 0 0 0.05 0.06 0.05 0 0 0 – 0 0.09 – 0.22 0.07 0 0 0 0 0 0
Vac/Vac Membrane 0.38 0.27 0.16 0.09 0.22 0.08 0.05 0 0 0 0 0 – 0.38 0.41 – 0.14 0.21 0.05 0.1 0 0.08 0.06 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 0 0 0 0 0 – 3 3 – 3 1 0 2 5 1 7 5
Bud 0 4 2 2 2 1 9 14 10 12 11 19 – 4 4 – 8 3 1 0 0 2 2 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – 2 0 1 3 1 2 6 8
Bud Site 0 1 0 0 3 5 8 14 21 20 12 14 – 0 0 – 2 0 – – – – – –
Cell Periphery 2 3 2 1 0 0 5 0 1 2 0 1 – 1 3 – 1 3 0 0 0 0 0 0
Cytoplasm 5 1 12 11 21 12 13 19 7 7 5 4 – 12 15 – 17 24 0 0 0 1 0 0
Endoplasmic Reticulum 2 1 4 3 0 2 4 0 0 0 1 0 – 3 1 – 4 4 0 0 1 0 2 1
Endosome 21 2 15 8 25 5 11 2 8 1 3 9 – 18 24 – 11 13 24 22 22 10 25 37
Golgi 3 0 2 0 0 0 0 0 0 0 0 0 – 2 4 – 1 6 6 6 6 2 10 10
Mitochondria 185 225 196 146 165 215 397 351 351 521 314 507 – 47 47 – 12 14 193 170 161 113 185 134
Nucleus 4 3 2 3 6 26 60 50 16 22 7 9 – 3 4 – 1 3 1 3 0 1 1 1
Nuclear Periphery 7 8 13 14 16 24 68 28 47 68 35 60 – 0 1 – 0 0 4 2 4 6 3 3
Nucleolus 4 14 11 5 14 30 92 79 75 104 61 108 – 2 1 – 3 1 0 2 1 1 3 4
Peroxisomes 2 0 8 3 5 2 11 4 7 6 0 7 – 1 7 – 11 1 6 3 12 8 15 23
SpindlePole 1 2 3 3 2 4 26 27 18 20 12 21 – 2 14 – 19 5 2 4 2 0 4 5
Vac/Vac Membrane 90 68 37 15 53 23 24 15 14 19 8 22 – 39 62 – 12 15 14 24 5 13 16 14
Unique Cell Count 239 252 229 172 245 271 529 449 399 572 347 579 103 151 88 72 259 249 231 172 290 256
Labelled Cell Count 326 333 307 214 312 349 728 603 575 802 469 781 137 190 107 93 259 249 231 172 290 256


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.0 6.0 6.2 5.7 5.4 4.8 4.5 4.5 4.5 4.0 4.3 4.5 – 6.5 6.7 4.9 5.2 6.9 6.7 7.1 7.6
Std Deviation (1e-4) 1.3 0.9 2.0 1.7 1.6 1.7 1.4 1.5 1.1 1.2 1.1 1.2 – 1.1 1.3 1.2 1.3 2.1 1.4 1.3 1.9
Intensity Change (Log2) – – – -0.12 -0.19 -0.36 -0.46 -0.46 -0.46 -0.61 -0.54 -0.46 – 0.07 0.13 -0.35 -0.25 0.16 0.11 0.2 0.3


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 – 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 – 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 – 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 – 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 – 0 0
Cytoplasm 0.5 1.4 -0.4 -2.0 -0.6 -2.5 -3.4 -2.6 0 – 2.1 1.7 – 3.9 6.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 – 0 0
Endosome -0.8 1.4 0 0 0 0 0 0 0 – 3.1 2.9 – 1.7 2.9
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 – 0 0
Mitochondria -0.2 -4.7 0 0 0 0 0 0 0 – -7.6 -10.8 – -12.1 -10.7
Nucleus 0 0 4.2 4.8 4.7 2.3 2.2 0 0 – 0 0 – 0 0
Nuclear Periphery 1.0 0.4 0 0 0 0 0 0 0 – 0 -2.5 – 0 0
Nucleolus -1.0 0.4 0 0 0 0 0 0 0 – 0 0 – 0 0
Peroxisomes 0 -1.0 0 0 0 0 0 0 0 – 0 0.6 – 3.0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 3.7 – 6.4 0
Vacuole -2.2 1.5 -2.6 -5.4 -5.9 -5.6 -6.4 -6.1 -6.1 – 4.4 5.4 – -0.6 0.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.8756 1.774 1.6079 1.5511 1.7383 1.5866 3.8372 3.8537 3.6521 3.4355 3.381 3.695 3.5412 3.8937 3.6646 3.4385 3.4648 3.3628
Actin 0.0149 0.0184 0.009 0.0024 0.007 0.0097 0.0256 0.0144 0.0069 0.0004 0.0159 0.0053 0.02 0.0075 0.0124 0.0013 0.0032 0.0096
Bud 0.0014 0.0043 0.0013 0.0026 0.0027 0.001 0.0014 0.0044 0.0016 0.0004 0.0007 0.0007 0.0011 0.0027 0.002 0.0012 0.0049 0.0007
Bud Neck 0.0049 0.001 0.0011 0.0051 0.0027 0.0046 0.0073 0.0021 0.0107 0.0002 0.0009 0.0013 0.0032 0.0011 0.001 0.0014 0.0005 0.0014
Bud Periphery 0.004 0.0048 0.0023 0.0072 0.0066 0.0028 0.0031 0.0058 0.0031 0.0007 0.0008 0.0026 0.0027 0.0036 0.0042 0.0062 0.0127 0.0024
Bud Site 0.0068 0.0405 0.0111 0.0039 0.01 0.0018 0.0079 0.0457 0.0078 0.0003 0.0018 0.0009 0.0119 0.0249 0.0093 0.0015 0.0023 0.0012
Cell Periphery 0.0011 0.0007 0.0006 0.001 0.0005 0.0008 0.0011 0.0015 0.0008 0.0001 0.0002 0.0006 0.0015 0.0008 0.0014 0.0007 0.0006 0.0004
Cytoplasm 0.0057 0.0072 0.0062 0.0024 0.0028 0.0043 0.0167 0.0186 0.0081 0.0007 0.0069 0.0049 0.0058 0.0102 0.0092 0.0017 0.0059 0.0041
Cytoplasmic Foci 0.0334 0.0526 0.0421 0.0196 0.043 0.0226 0.0262 0.0588 0.0395 0.0208 0.0788 0.0264 0.0357 0.0536 0.0328 0.0254 0.0272 0.0183
Eisosomes 0.0005 0.0003 0.0003 0.0002 0.0003 0.0003 0.0005 0.0004 0.0003 0.0001 0.0002 0.0003 0.001 0.0003 0.0004 0.0002 0.0002 0.0002
Endoplasmic Reticulum 0.0051 0.0048 0.0016 0.0007 0.0005 0.0019 0.0068 0.0021 0.0011 0.0002 0.0052 0.0013 0.0058 0.0019 0.0013 0.0003 0.0006 0.0013
Endosome 0.141 0.1217 0.1209 0.0724 0.1717 0.1154 0.1618 0.1817 0.114 0.0765 0.2517 0.0475 0.1422 0.1482 0.0815 0.0803 0.1054 0.0745
Golgi 0.0739 0.0751 0.061 0.061 0.0602 0.0586 0.0579 0.0856 0.0861 0.0257 0.1498 0.0502 0.0763 0.1053 0.0725 0.071 0.0945 0.0545
Lipid Particles 0.0257 0.0307 0.0207 0.0068 0.0024 0.0177 0.0323 0.0277 0.0087 0.0043 0.027 0.0107 0.055 0.0076 0.0109 0.0157 0.0041 0.0097
Mitochondria 0.5709 0.5675 0.6332 0.7642 0.6642 0.6918 0.5309 0.4741 0.6603 0.8516 0.3965 0.795 0.5652 0.5689 0.7059 0.7499 0.6795 0.7883
None 0.0138 0.0007 0.007 0.0012 0.0004 0.0072 0.0286 0.0118 0.0007 0.0002 0.001 0.0124 0.0044 0.0009 0.007 0.0003 0.0263 0.0008
Nuclear Periphery 0.0164 0.005 0.0041 0.0005 0.0003 0.0018 0.0138 0.0012 0.0004 0.0001 0.0024 0.0009 0.0057 0.0006 0.0007 0.0001 0.0002 0.0002
Nucleolus 0.0015 0.0006 0.0006 0.0007 0.0007 0.0004 0.0022 0.0019 0.0003 0.0001 0.0003 0.0003 0.0027 0.0005 0.0003 0.0005 0.0002 0.0002
Nucleus 0.0119 0.0012 0.0044 0.0007 0.0007 0.0006 0.004 0.0014 0.0005 0.0001 0.0006 0.0004 0.0012 0.0005 0.0004 0.0005 0.0003 0.0002
Peroxisomes 0.0265 0.0373 0.0421 0.0341 0.0132 0.0289 0.025 0.0243 0.0281 0.0112 0.0502 0.0247 0.0241 0.0347 0.0272 0.0186 0.0227 0.0217
Punctate Nuclear 0.0035 0.0005 0.0023 0.0001 0.0002 0.0002 0.0117 0.0007 0.0003 0.0001 0.0017 0.0002 0.0007 0.0002 0.0013 0.0001 0.0001 0.0001
Vacuole 0.0205 0.0129 0.0166 0.0072 0.0055 0.0137 0.0233 0.0256 0.0128 0.0036 0.0043 0.0075 0.0216 0.0162 0.0103 0.0166 0.0046 0.0049
Vacuole Periphery 0.0165 0.012 0.0116 0.0059 0.0046 0.0138 0.0119 0.0105 0.0079 0.0025 0.0032 0.0059 0.0121 0.0098 0.0078 0.0065 0.004 0.0053

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.6318 9.197 8.5307 13.0557 12.0051 13.8813 14.9947 14.9061 15.4798 19.308
Translational Efficiency 0.5572 1.0008 0.7607 0.4879 0.6535 0.5702 0.5118 0.5523 0.4505 0.4593

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Peripheral protein of cytosolic face of mitochondrial outer membrane; required for mitochondrial fission; interacts with Fis1p and with the self-assembled oligomeric form of the dynamin-related GTPase Dnm1p; contains WD repeats; MDV1 has a paralog, CAF4, that arose from the whole genome duplication
Localization
Cell Percentages mitochondrion (90%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Mdv1

Mdv1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mdv1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available