Standard name
Human Ortholog
Description D-ribulose-5-phosphate 3-epimerase; catalyzes a reaction in the non-oxidative part of the pentose-phosphate pathway; mutants are sensitive to oxidative stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.99 0.96 0.88 0.82 0.81 – 0.71 0.69 0.67 0.57 0.99 0.98 0.99 0.94 0.92 0.98 0.94 0.93 0.96 0.9 0.91 0.86
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.19 0 – 0.21 0.13 0.24 0.26 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0.05 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.11 0.06 0.2 – 0.14 0.19 0.13 0.23 0 0 0 0 0.09 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 0 4 0 1 2 2
Bud 0 0 0 0 0 3 2 – 0 1 3 7 0 0 0 0 0 0 6 5 0 0 12 12
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 3
Bud Site 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 2 4 0 6 1 6 – 5 10 3 10 3 1 0 8 10 4 0 0 0 0 0 0
Cytoplasm 469 529 266 196 246 182 397 – 272 281 262 308 288 481 399 264 235 187 446 505 257 165 449 305
Endoplasmic Reticulum 2 2 1 1 3 2 0 – 5 4 4 3 0 1 1 12 5 4 0 1 0 3 3 4
Endosome 2 1 1 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 4 3 0 1 2 1
Golgi 1 1 0 1 0 0 0 – 0 0 0 0 2 2 0 2 2 0 6 2 0 1 3 4
Mitochondria 1 3 0 3 5 42 6 – 80 53 94 139 1 0 2 2 2 0 0 1 1 2 1 3
Nucleus 1 2 0 3 7 2 13 – 12 12 18 15 1 1 1 8 2 9 1 5 0 0 2 2
Nuclear Periphery 1 0 0 0 0 0 0 – 2 1 0 4 0 0 0 0 0 0 0 0 0 0 0 2
Nucleolus 0 1 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 2 – 0 1 0 0 0 0 0 0 0 0 1 2 2 0 6 2
Vac/Vac Membrane 8 9 2 6 32 13 98 – 54 79 51 126 2 8 8 12 22 1 2 4 0 2 4 2
Unique Cell Count 481 542 270 204 280 221 493 385 409 389 541 292 490 405 281 255 191 477 543 269 183 493 354
Labelled Cell Count 486 550 274 210 299 245 524 430 443 435 614 297 494 411 308 278 205 477 543 269 183 493 354


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.5 7.7 7.6 7.3 7.9 5.1 7.4 – 6.4 7.1 6.4 6.8 8.7 8.9 9.1 15.7 16.3 15.3 7.9 8.1 8.3
Std Deviation (1e-4) 1.5 1.6 1.3 1.6 1.7 1.2 1.8 – 1.4 1.5 1.3 1.5 1.9 2.0 2.0 3.2 3.2 3.5 2.0 2.1 2.0
Intensity Change (Log2) – – – -0.07 0.05 -0.58 -0.05 – -0.26 -0.1 -0.25 -0.17 0.19 0.22 0.25 1.05 1.09 1.0 0.05 0.08 0.13


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0.9 0 0 0 0 0 1.1 1.7 0
Cytoplasm -1.7 -4.9 -6.3 -7.0 – -9.2 -9.6 -9.9 -12.3 0.1 -0.4 0 -2.8 -3.5 -0.5
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 3.0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 7.5 0 – 8.0 6.2 8.7 9.1 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 3.6 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 5.2 3.3 7.5 – 6.0 7.3 5.7 8.3 0 0 0 2.6 4.3 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.2472 4.7374 4.6003 4.3686 4.5164 4.5764 2.4041 4.3524 2.9389 2.8635 2.521 3.3339 4.0462 5.5697 5.0672 5.3642 5.0889 5.106
Actin 0.0003 0.0013 0.0008 0.0009 0.0005 0.0009 0.0056 0.0001 0.0007 0.0017 0.0003 0.0013 0.0213 0.0008 0.0108 0.0017 0.0079 0.0024
Bud 0.0001 0.0009 0.0004 0.0002 0.0112 0.0013 0.001 0 0.0001 0.0001 0.0001 0.0001 0.0015 0.0012 0.0022 0.0041 0.0008 0.0002
Bud Neck 0.0004 0.0003 0.0003 0.0003 0.0009 0.0014 0.0025 0.0001 0.0002 0.0005 0.0002 0.0007 0.0036 0.0002 0.001 0.0008 0.0008 0.0009
Bud Periphery 0 0.0005 0.0004 0.0001 0.0034 0.0008 0.0009 0 0.0001 0.0001 0 0 0.0014 0.0002 0.0004 0.0031 0.0005 0.0001
Bud Site 0.0002 0.0078 0.0005 0.0002 0.0015 0.0003 0.0446 0.0001 0.0004 0.0002 0.0001 0.0001 0.0061 0.004 0.0015 0.0011 0.0009 0.0001
Cell Periphery 0.0002 0.0002 0.0002 0 0.0002 0.0001 0.0005 0 0.0002 0.0001 0 0 0.0004 0.0001 0.0001 0.0002 0.0001 0
Cytoplasm 0.7738 0.8653 0.819 0.8229 0.871 0.8327 0.4956 0.8704 0.6881 0.6771 0.9516 0.7964 0.796 0.9224 0.8572 0.8831 0.7683 0.8723
Cytoplasmic Foci 0.0038 0.0058 0.0043 0.0084 0.0071 0.0097 0.0079 0.0011 0.0039 0.0081 0.0059 0.011 0.028 0.0042 0.0112 0.0094 0.0358 0.0064
Eisosomes 0.0001 0 0 0 0 0 0.0002 0 0 0.0001 0 0 0.0003 0 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.0043 0.0023 0.0033 0.0027 0.0018 0.0021 0.0061 0.0006 0.0032 0.0049 0.0024 0.0013 0.0042 0.0015 0.0034 0.0032 0.0042 0.0024
Endosome 0.0015 0.0015 0.0021 0.0062 0.0018 0.0032 0.0196 0.0003 0.0013 0.021 0.0018 0.0029 0.0055 0.0015 0.0033 0.0029 0.0082 0.0051
Golgi 0.0001 0.0002 0.0002 0.0003 0.0001 0.0002 0.0034 0 0.0003 0.0033 0.0001 0.0015 0.0016 0.0001 0.001 0.0004 0.0017 0.0004
Lipid Particles 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0034 0 0.0001 0.035 0 0.0052 0.0008 0 0.0006 0.0003 0.0049 0.0001
Mitochondria 0.0001 0.0002 0.0004 0.0002 0.0003 0.0004 0.0014 0 0.0002 0.0054 0.0001 0.0008 0.0008 0.0006 0.0003 0.0003 0.0108 0.0003
None 0.1929 0.1064 0.155 0.149 0.0699 0.1291 0.3322 0.1252 0.298 0.2345 0.035 0.1662 0.1084 0.0554 0.096 0.0798 0.1088 0.101
Nuclear Periphery 0.0012 0.001 0.0015 0.0014 0.0016 0.0011 0.0543 0.0003 0.0008 0.0025 0.0004 0.0003 0.002 0.0009 0.0011 0.0014 0.0021 0.0014
Nucleolus 0.0001 0.0001 0.0001 0 0.0004 0.0002 0.0009 0 0 0.0001 0 0 0.0003 0.0001 0.0005 0.0001 0.0003 0.0001
Nucleus 0.019 0.0037 0.0091 0.0045 0.0195 0.0101 0.0041 0.0012 0.0014 0.0012 0.001 0.0013 0.005 0.0045 0.0054 0.0049 0.0048 0.0045
Peroxisomes 0 0.0003 0.0002 0.0002 0.0001 0.0002 0.0013 0 0.0001 0.002 0.0001 0.0089 0.0069 0.0001 0.0009 0.0004 0.0186 0.0003
Punctate Nuclear 0.0006 0.0007 0.0007 0.0008 0.007 0.0048 0.0014 0.0001 0.0004 0.0009 0.0003 0.0015 0.0047 0.0009 0.0024 0.0015 0.0186 0.001
Vacuole 0.0009 0.0012 0.0012 0.0014 0.0015 0.0011 0.0064 0.0002 0.0005 0.0009 0.0004 0.0002 0.001 0.0009 0.0005 0.0012 0.0011 0.0008
Vacuole Periphery 0.0001 0.0001 0.0002 0.0002 0.0002 0.0002 0.0069 0 0.0001 0.0005 0.0001 0.0001 0.0002 0.0001 0.0001 0.0002 0.0006 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 200.8288 213.2333 176.6032 164.8729 184.5629 203.0248 183.26 170.8633 184.0141 170.4239
Translational Efficiency 1.0927 1.1272 1.2663 1.4955 1.4826 1.1754 1.2975 1.4131 1.2534 1.3208

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description D-ribulose-5-phosphate 3-epimerase; catalyzes a reaction in the non-oxidative part of the pentose-phosphate pathway; mutants are sensitive to oxidative stress
Localization
Cell Percentages cytoplasm (80%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Rpe1

Rpe1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpe1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available