Standard name
Human Ortholog
Description Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit; interacts directly with Arx1p; responsible for Tif6p recycling defects in absence of Rei1p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0 0 0.05 0 0 0 0 0 0 0 0 0.05 0.1 0.14 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.08 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.9 0.93 0.94 0.71 0.87 0.77 0.84 0.77 0.8 0.82 0.82 0.77 0.9 0.83 0.71 0.82 0.76 0.79 0.93 0.94 0.96 0.8 0.74 0.66
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0.29 0.06 0.08 0.07 0.12 0.1 0.09 0.12 0.12 0 0.08 0.14 0 0.05 0 0 0 0 0.05 0.07 0.09
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.08 0.05 0 0 0 0.11 0.06 0.12 0.09 0.07 0.06 0.08 0 0 0 0.14 0.18 0.17 0 0 0 0.13 0.16 0.21
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 0 0 0 0 0 0 0 4 0 0 1 0 0 0 0 0 0
Bud 0 0 0 0 1 1 0 1 0 2 1 0 0 1 3 0 0 1 4 0 0 2 6 13
Bud Neck 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 1 0 1 2 2 1 4 0 0 4 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 12 4 5 14 19 15 23 16 15 18 15 15 43 102 161 4 8 8 0 0 0 4 3 4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 2 2 1 0 0 0 0 0
Endosome 0 0 0 0 0 3 2 6 1 5 4 2 0 8 15 2 4 1 2 0 0 5 4 11
Golgi 0 0 0 0 1 0 1 0 0 0 0 0 0 0 2 1 3 2 0 0 0 3 1 0
Mitochondria 1 0 0 0 0 26 1 12 8 16 17 28 2 2 4 3 2 2 0 0 0 0 6 5
Nucleus 216 182 117 207 405 267 508 591 347 515 363 406 716 878 835 268 236 256 228 182 123 679 815 752
Nuclear Periphery 0 0 0 0 9 3 8 5 8 15 5 4 0 8 4 0 1 0 0 0 0 1 3 4
Nucleolus 2 0 0 84 30 27 40 91 43 58 53 63 21 83 160 14 15 14 3 0 0 43 79 100
Peroxisomes 0 0 0 0 0 0 0 1 0 0 0 0 0 5 10 1 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 3 2 0 2 1 1 0 8 25 1 1 0 0 0 0 1 1 9
Vac/Vac Membrane 19 10 4 7 15 37 37 92 39 47 25 43 26 28 40 46 56 55 3 8 2 107 177 240
Unique Cell Count 240 195 125 290 463 346 603 767 434 631 442 524 792 1063 1177 328 311 325 246 193 129 854 1106 1147
Labelled Cell Count 250 196 126 312 481 380 623 820 463 680 485 566 809 1123 1267 343 328 342 246 193 129 854 1106 1147


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 18.6 19.5 18.6 19.2 15.8 9.0 11.0 10.2 10.3 10.2 10.0 9.8 21.3 20.7 18.5 26.0 23.6 27.3 19.5 19.0 19.0
Std Deviation (1e-4) 5.1 4.0 4.5 5.9 5.7 2.6 2.2 2.4 3.2 3.1 2.7 2.4 6.8 9.6 9.2 7.2 7.6 7.7 5.1 5.8 5.4
Intensity Change (Log2) – – – 0.05 -0.24 -1.05 -0.75 -0.87 -0.86 -0.87 -0.9 -0.93 0.2 0.15 -0.01 0.48 0.34 0.55 0.07 0.03 0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.4 0.1 0.2 0 0 0 0 0 0 0.7 2.1 3.1 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.2 0 0 0 0 0 2.6 0 0 0 0 0 0
Nucleus -5.0 -1.9 -4.1 -2.7 -4.2 -3.6 -3.3 -3.1 -4.1 -1.2 -3.2 -5.4 -3.2 -4.3 -3.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 6.7 2.9 3.2 3.0 4.1 3.7 3.5 4.1 4.1 0 3.2 4.4 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 2.5 1.3 2.9 2.1 1.7 1.1 1.9 0 0 0 3.3 4.1 3.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 11.7142 12.4733 11.7294 10.8727 10.8079 11.1472 14.6811 17.7284 15.8983 14.6346 17.3182 15.7497 14.8309 19.0877 17.4569 16.273 15.3455 17.0684
Actin 0.0001 0 0 0 0 0 0.0088 0.0001 0.0017 0.0012 0.0008 0.0002 0.0283 0 0.0046 0.015 0.0026 0.0071
Bud 0 0 0 0 0 0 0.0003 0.0001 0.0001 0.0002 0 0.0001 0.0006 0 0.0001 0.0007 0.0003 0.0003
Bud Neck 0.0001 0.0001 0.0001 0.001 0.0006 0.0005 0.0004 0.0001 0.0001 0.0002 0.0001 0.0007 0.0008 0.0001 0.0003 0.0001 0.0015 0.0007
Bud Periphery 0.0001 0 0 0.0002 0.0001 0 0.0004 0.0002 0.0003 0.0004 0 0.0001 0.0013 0 0.0003 0.0006 0.0004 0.0008
Bud Site 0.0001 0 0 0 0 0 0.0008 0.0005 0.0003 0.0005 0 0.0001 0.0017 0.0001 0.0006 0.003 0.001 0.0006
Cell Periphery 0 0 0 0 0 0 0.0003 0.0002 0.0002 0.0006 0 0.0001 0.0007 0 0.0002 0.0003 0.0002 0.0001
Cytoplasm 0.0024 0.0001 0.0001 0.0001 0.0001 0.0001 0.0013 0.0007 0.0012 0.0017 0.0001 0.0013 0.0097 0.0042 0.0068 0.003 0.0017 0.0008
Cytoplasmic Foci 0.0003 0 0 0 0 0 0.0103 0.0001 0.0036 0.0054 0.0007 0.003 0.0087 0 0.0123 0.0243 0.003 0.0011
Eisosomes 0 0 0 0 0 0 0.0001 0 0.0001 0 0 0 0.0006 0 0.0001 0.0001 0.0001 0
Endoplasmic Reticulum 0.0003 0 0 0.0001 0 0 0.0014 0 0.0005 0.0019 0.0004 0.0003 0.0036 0 0.0022 0.0014 0.0028 0.0003
Endosome 0.0008 0 0 0 0.0001 0 0.0077 0.0001 0.0015 0.0085 0.0119 0.0027 0.015 0 0.0053 0.0026 0.0277 0.0029
Golgi 0 0 0 0 0 0 0.0046 0 0.0006 0.0008 0.0202 0.0016 0.0033 0 0.0021 0.0014 0.0041 0.0021
Lipid Particles 0.0004 0 0 0 0 0 0.0154 0.0002 0.0029 0.0047 0.0048 0.0032 0.0081 0 0.011 0.0078 0.0105 0.0022
Mitochondria 0.0003 0.0001 0.0002 0.0001 0.0003 0.0001 0.0061 0.0008 0.0077 0.005 0.0017 0.0006 0.0069 0.0003 0.0049 0.0056 0.0018 0.0019
None 0.0002 0 0 0 0 0 0.001 0 0.0005 0.0012 0 0.0003 0.0042 0 0.0055 0.0049 0.0014 0.0003
Nuclear Periphery 0.0048 0.0027 0.0032 0.006 0.003 0.001 0.0102 0.0043 0.0051 0.0284 0.002 0.0039 0.0195 0.0076 0.0082 0.0039 0.0107 0.0034
Nucleolus 0.0156 0.0131 0.0141 0.0095 0.0145 0.0251 0.0343 0.0139 0.0314 0.0295 0.0072 0.0299 0.0376 0.0151 0.0328 0.0039 0.0296 0.0259
Nucleus 0.9721 0.9836 0.9819 0.9798 0.9801 0.9727 0.8652 0.9765 0.9348 0.8799 0.9487 0.9449 0.822 0.9716 0.8843 0.8975 0.8917 0.9413
Peroxisomes 0 0 0 0 0 0 0.014 0 0.0008 0.0002 0.0001 0.0008 0.0011 0 0.0099 0.0134 0.0009 0.0005
Punctate Nuclear 0.0017 0.0001 0.0001 0.0017 0.0005 0.0002 0.0121 0.0002 0.0009 0.0169 0.0002 0.0042 0.0146 0.0001 0.006 0.0064 0.0015 0.0023
Vacuole 0.0004 0.0001 0.0002 0.0011 0.0003 0.0001 0.0028 0.0013 0.0036 0.0086 0.0003 0.0014 0.0079 0.0004 0.0012 0.0024 0.0048 0.0041
Vacuole Periphery 0.0003 0.0001 0.0002 0.0003 0.0002 0.0001 0.0025 0.0006 0.0023 0.0041 0.0006 0.0005 0.0038 0.0004 0.0015 0.0015 0.0018 0.0011

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 112.8482 110.2201 90.1055 59.3285 90.768 100.254 57.6106 44.6201 42.9226 73.6614
Translational Efficiency 2.4348 2.332 2.17 2.9472 2.2866 1.8726 2.1581 2.9489 2.0329 2.0513

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit; interacts directly with Arx1p; responsible for Tif6p recycling defects in absence of Rei1p
Localization
Cell Percentages nucleus (100%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-5

Alb1

Alb1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Alb1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available