Standard name | |
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Human Ortholog | |
Description | Subunit of tRNA (1-methyladenosine) methyltransferase; required, along with Gcd10p, for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 |
Bud | – | – | 0 | 0 | 2 | 1 | 6 | 5 | 3 | 6 | 0 | 6 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 1 | 1 | 1 | 3 |
Bud Neck | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 |
Bud Site | – | – | 2 | 1 | 1 | 4 | 15 | 16 | 11 | 12 | 18 | 27 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | – | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 |
Cytoplasm | – | – | 5 | 18 | 10 | 14 | 17 | 30 | 11 | 9 | 6 | 6 | 16 | 42 | 78 | 15 | 22 | 8 | 0 | – | 2 | 0 | 3 | 4 |
Endoplasmic Reticulum | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 5 | 3 | 0 | – | 0 | 0 | 0 | 0 |
Endosome | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | – | 3 | 0 | 3 | 4 |
Golgi | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | – | 3 | 0 | 2 | 0 |
Mitochondria | – | – | 16 | 9 | 20 | 134 | 322 | 160 | 132 | 224 | 227 | 250 | 1 | 2 | 1 | 2 | 8 | 3 | 1 | – | 2 | 0 | 1 | 2 |
Nucleus | – | – | 385 | 277 | 397 | 495 | 755 | 804 | 411 | 561 | 473 | 618 | 276 | 381 | 417 | 143 | 168 | 121 | 0 | – | 367 | 181 | 370 | 329 |
Nuclear Periphery | – | – | 4 | 3 | 3 | 8 | 9 | 29 | 6 | 12 | 9 | 24 | 3 | 10 | 2 | 1 | 5 | 0 | 0 | – | 0 | 0 | 1 | 2 |
Nucleolus | – | – | 4 | 4 | 5 | 22 | 57 | 72 | 49 | 64 | 86 | 98 | 0 | 0 | 3 | 0 | 4 | 6 | 0 | – | 1 | 0 | 3 | 9 |
Peroxisomes | – | – | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 |
SpindlePole | – | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6 | 3 | 0 | 1 | 3 | 0 | – | 1 | 0 | 0 | 2 |
Vac/Vac Membrane | – | – | 0 | 2 | 5 | 2 | 13 | 12 | 1 | 12 | 3 | 5 | 0 | 1 | 6 | 9 | 7 | 7 | 0 | – | 15 | 4 | 31 | 47 |
Unique Cell Count | 396 | 301 | 420 | 565 | 884 | 940 | 484 | 694 | 583 | 780 | 294 | 431 | 506 | 166 | 201 | 143 | 4 | 402 | 192 | 425 | 410 | |||
Labelled Cell Count | 419 | 315 | 443 | 680 | 1194 | 1128 | 624 | 900 | 822 | 1035 | 297 | 446 | 513 | 172 | 220 | 152 | 4 | 402 | 192 | 425 | 410 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | – | – | 6.6 | 6.6 | 5.9 | 4.8 | 4.6 | 4.8 | 4.7 | 4.6 | 4.3 | 4.4 | 8.0 | 7.4 | 7.3 | 10.2 | 9.7 | 11.0 | 7.7 | 8.3 | 8.7 |
Std Deviation (1e-4) | – | – | 1.6 | 1.9 | 1.6 | 1.1 | 1.0 | 1.3 | 1.5 | 1.2 | 1.0 | 0.9 | 1.4 | 1.5 | 1.6 | 2.6 | 2.6 | 3.8 | 1.3 | 1.5 | 1.6 |
Intensity Change (Log2) | – | – | – | 0.01 | -0.16 | -0.45 | -0.52 | -0.44 | -0.47 | -0.51 | -0.62 | -0.59 | 0.28 | 0.17 | 0.15 | 0.64 | 0.56 | 0.74 | 0.23 | 0.33 | 0.4 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3.5 | 1.2 | 1.3 | 0.8 | 2.0 | 1.1 | 0 | 0 | 0 | 3.2 | 5.3 | 7.3 | 4.5 | 5.4 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -0.7 | 0.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -3.1 | -3.5 | -4.2 | -1.7 | 0 | -1.1 |
Nucleus | -3.1 | -1.9 | -5.3 | -6.3 | -6.2 | -6.2 | -7.7 | -7.5 | -8.2 | -2.2 | -4.8 | -7.0 | -5.0 | -6.0 | -5.4 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.5 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.4151 | 4.7705 | 4.3147 | 4.0037 | 3.7646 | 4.2638 | 3.9186 | 4.6337 | 4.4779 | 3.884 | 3.5475 | 4.1039 | 3.0769 | 3.2733 | 2.8789 | 2.2792 | 2.4817 | 2.6446 |
Actin | 0.006 | 0 | 0.0043 | 0.0007 | 0.036 | 0.0018 | 0.0273 | 0 | 0.014 | 0.0038 | 0.0135 | 0.0107 | 0.0303 | 0 | 0.004 | 0.0355 | 0.0052 | 0.0001 |
Bud | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0007 | 0 | 0.0009 | 0.0003 | 0.0014 | 0.0001 | 0.0004 | 0 | 0.0001 | 0.0001 | 0.0021 | 0 |
Bud Neck | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0006 | 0.0003 | 0.004 | 0 | 0.0004 | 0.0038 | 0.001 | 0.0007 | 0.001 | 0 | 0.0001 | 0.0002 | 0.0028 | 0.0004 |
Bud Periphery | 0.0002 | 0.0003 | 0.0002 | 0.0004 | 0.0004 | 0.0001 | 0.0015 | 0 | 0.0018 | 0.0004 | 0.0029 | 0.0002 | 0.0007 | 0 | 0.0002 | 0.0002 | 0.0055 | 0 |
Bud Site | 0.0001 | 0.0001 | 0.0007 | 0.0002 | 0.0005 | 0 | 0.008 | 0 | 0.0021 | 0.0045 | 0.0053 | 0.0002 | 0.0014 | 0 | 0.0001 | 0.0004 | 0.0053 | 0 |
Cell Periphery | 0 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0 | 0.0009 | 0 | 0.0003 | 0.0002 | 0.0004 | 0 | 0.0002 | 0 | 0 | 0 | 0.0016 | 0 |
Cytoplasm | 0.0006 | 0 | 0.0001 | 0.0002 | 0.0013 | 0 | 0.0036 | 0.0001 | 0.0003 | 0.0005 | 0.0043 | 0.0003 | 0.0064 | 0 | 0.0001 | 0.0001 | 0.0028 | 0.0003 |
Cytoplasmic Foci | 0.0086 | 0 | 0.0007 | 0.0004 | 0.0017 | 0.0002 | 0.0103 | 0.0002 | 0.0016 | 0.0246 | 0.007 | 0.0019 | 0.0062 | 0 | 0.0022 | 0.0181 | 0.0075 | 0.0004 |
Eisosomes | 0.0001 | 0 | 0 | 0.0001 | 0.001 | 0 | 0.0005 | 0 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0013 | 0 | 0.0002 | 0.0012 | 0.0012 | 0.0002 | 0.0045 | 0.0004 | 0.0015 | 0.0029 | 0.0037 | 0.0009 | 0.0042 | 0 | 0.0001 | 0.0001 | 0.0018 | 0.0001 |
Endosome | 0.0049 | 0 | 0.0015 | 0.0021 | 0.0047 | 0.0014 | 0.0154 | 0.0043 | 0.0036 | 0.0262 | 0.0409 | 0.0098 | 0.0126 | 0 | 0.0013 | 0.0046 | 0.0147 | 0.0005 |
Golgi | 0.0042 | 0 | 0.0008 | 0.0008 | 0.0034 | 0.0011 | 0.0041 | 0.0006 | 0.0026 | 0.013 | 0.0123 | 0.0025 | 0.0041 | 0 | 0.0011 | 0.0039 | 0.0017 | 0 |
Lipid Particles | 0.0071 | 0 | 0.002 | 0.0093 | 0.0027 | 0.0007 | 0.0127 | 0.0002 | 0.0071 | 0.0117 | 0.0244 | 0.0033 | 0.0052 | 0 | 0.0045 | 0.0477 | 0.0037 | 0.0001 |
Mitochondria | 0.0015 | 0.0064 | 0.0006 | 0.0119 | 0.0026 | 0.0005 | 0.0162 | 0.0002 | 0.0069 | 0.0314 | 0.019 | 0.0112 | 0.0067 | 0.0001 | 0.0012 | 0.0012 | 0.0064 | 0.0002 |
None | 0.0004 | 0 | 0.0002 | 0.0004 | 0.0176 | 0 | 0.0036 | 0 | 0.0003 | 0.0002 | 0.0038 | 0.0004 | 0.0031 | 0 | 0.0002 | 0.0002 | 0.0025 | 0.0002 |
Nuclear Periphery | 0.0087 | 0.0008 | 0.0058 | 0.0075 | 0.0066 | 0.0016 | 0.0373 | 0.0021 | 0.0045 | 0.0127 | 0.0289 | 0.0036 | 0.0134 | 0.0007 | 0.0007 | 0.0002 | 0.0195 | 0.0052 |
Nucleolus | 0.009 | 0.0068 | 0.0029 | 0.0147 | 0.068 | 0.0198 | 0.0144 | 0.005 | 0.0035 | 0.0047 | 0.0555 | 0.0147 | 0.0067 | 0.0064 | 0.0035 | 0.0025 | 0.0315 | 0.0185 |
Nucleus | 0.9397 | 0.9846 | 0.9764 | 0.9432 | 0.8436 | 0.9714 | 0.8122 | 0.9861 | 0.9404 | 0.8362 | 0.7359 | 0.9346 | 0.885 | 0.9927 | 0.9762 | 0.8611 | 0.8577 | 0.9694 |
Peroxisomes | 0.0047 | 0 | 0.0005 | 0.0002 | 0.0017 | 0.0001 | 0.0046 | 0 | 0.0067 | 0.0155 | 0.005 | 0.0013 | 0.0039 | 0 | 0.0026 | 0.0229 | 0.0037 | 0 |
Punctate Nuclear | 0.0015 | 0.0003 | 0.0018 | 0.0004 | 0.005 | 0.0004 | 0.0065 | 0.0001 | 0.0003 | 0.0005 | 0.0081 | 0.0017 | 0.0056 | 0.0001 | 0.0013 | 0.0007 | 0.019 | 0.0041 |
Vacuole | 0.0004 | 0.0002 | 0.0005 | 0.002 | 0.0004 | 0.0001 | 0.0062 | 0.0003 | 0.0007 | 0.003 | 0.0135 | 0.001 | 0.0015 | 0 | 0.0003 | 0.0001 | 0.0036 | 0.0002 |
Vacuole Periphery | 0.0008 | 0.0002 | 0.0003 | 0.004 | 0.0009 | 0.0002 | 0.0055 | 0.0003 | 0.0007 | 0.0039 | 0.013 | 0.0008 | 0.0012 | 0 | 0.0003 | 0.0001 | 0.0012 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 21.072 | 28.2556 | 22.8729 | 19.4906 | 22.9742 | 13.402 | 19.9118 | 18.1136 | 18.7578 | 22.304 |
Translational Efficiency | 1.9624 | 1.5677 | 1.5284 | 1.3564 | 1.4728 | 2.1141 | 1.8229 | 1.1445 | 1.0062 | 1.3609 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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