Standard name
Human Ortholog
Description Histone H3 acetylase with a role in transcriptional regulation; sequence-specific activator of histone genes, binds specifically and cooperatively to pairs of UAS elements in core histone promoters, functions at or near TATA box; involved in S phase-specific acetylation of H3K56 at histone promoters, which is required for recruitment of SWI/SNF nucleosome remodeling complex and subsequent transcription
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.07 0.12 0.17 0.27 0.23 0.21 0.24 – 0.24 0.18 0.17 0.12 0.12 0.28 0.33 0.22 0.15 0.16 0.05 0 0.05 0.05 0.11 0.07
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0.05 0.05 0 0 0 0 0 0
Endosome 0 0 0.12 0 0.14 0.08 0.06 – 0.07 0.05 0 0 0 0 0 0.05 0.08 0.11 0.06 0.05 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.3 0 0 0 0.06 0.26 0.28 – 0.49 0.5 0.68 0.65 0 0 0 0.06 0 0 0 0 0 0 0.06 0.05
Nucleus 0.61 0.68 0.53 0.57 0.4 0.34 0.39 – 0.29 0.37 0.33 0.39 0.88 0.64 0.56 0.54 0.56 0.57 0.5 0.57 0.49 0.56 0.4 0.35
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0.05 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0.05 0 0.07 – 0 0 0 0.06 0 0 0 0 0 0 0.12 0.07 0.06 0.07 0.09 0.12
Vac/Vac Membrane 0.19 0.2 0.24 0.08 0.24 0.17 0.13 – 0.05 0.11 0.06 0.06 0.05 0 0.09 0.2 0.22 0.22 0.12 0.14 0.17 0.18 0.19 0.2
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 0 2 1 0 0 0
Bud 0 0 0 0 0 3 3 – 2 5 6 4 0 0 0 0 0 1 2 10 2 1 6 6
Bud Neck 0 0 1 1 1 2 1 – 0 4 0 0 0 1 0 0 0 0 0 1 3 1 2 6
Bud Site 0 1 0 1 1 2 2 – 5 5 5 8 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Cytoplasm 10 35 25 32 37 47 45 – 41 33 35 19 5 51 59 79 40 48 8 10 9 5 25 15
Endoplasmic Reticulum 2 2 0 1 0 0 0 – 0 0 0 0 0 1 2 19 15 15 0 0 1 0 0 0
Endosome 3 9 17 2 23 18 11 – 11 10 1 1 0 3 5 18 23 32 11 18 5 0 7 6
Golgi 1 1 2 1 0 1 0 – 0 0 0 0 0 1 0 4 3 0 1 2 1 0 0 3
Mitochondria 42 11 3 5 9 57 54 – 83 93 143 106 0 0 0 21 6 10 6 7 8 2 15 10
Nucleus 86 206 76 67 64 74 75 – 49 69 70 64 37 116 101 192 152 169 94 199 97 62 94 75
Nuclear Periphery 0 2 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 0 3 0 0 0 1
Nucleolus 0 1 1 1 1 3 1 – 2 2 1 2 1 0 0 5 1 6 11 17 10 3 9 9
Peroxisomes 0 0 0 0 0 0 2 – 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
SpindlePole 1 8 6 5 8 7 14 – 6 6 3 10 1 3 7 2 7 2 23 24 12 8 21 25
Vac/Vac Membrane 27 60 34 9 38 38 24 – 9 20 12 10 2 7 17 71 60 65 22 48 34 20 45 43
Unique Cell Count 142 302 143 118 161 219 191 168 187 210 163 42 180 181 358 273 297 191 353 198 112 240 215
Labelled Cell Count 172 336 165 125 182 252 232 208 247 276 224 46 183 191 411 308 350 191 353 198 112 240 215


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.2 5.3 6.2 6.4 6.4 5.4 5.1 – 4.6 4.5 4.0 4.2 5.1 6.1 5.7 8.1 8.0 7.7 4.9 5.6 5.8
Std Deviation (1e-4) 0.6 1.0 1.5 2.1 2.0 1.7 1.5 – 1.9 1.5 1.4 1.6 0.6 1.3 1.1 1.9 2.1 1.8 0.6 1.3 1.3
Intensity Change (Log2) – – – 0.04 0.04 -0.22 -0.29 – -0.44 -0.48 -0.65 -0.57 -0.29 -0.04 -0.13 0.37 0.36 0.3 -0.36 -0.16 -0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.9 1.2 0.9 1.4 – 1.5 0 -0.2 -1.4 -0.9 2.3 3.1 1.1 -0.8 -0.3
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 2.8 2.9 0
Endosome -3.2 0.6 -1.2 -2.0 – 0 0 0 0 0 -3.8 -3.2 -2.7 -1.1 -0.3
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 1.6 6.0 6.3 – 0 0 0 0 0 0 0 1.8 0 0
Nucleus 0.6 -2.3 -3.7 -2.5 – -4.3 -2.9 -3.7 -2.4 4.1 2.1 0.5 0.1 0.5 0.7
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0.3 -0.5 1.2 – 0 0 0 0 0 0 -0.1 0 0 0
Vacuole -3.5 0 -1.5 -2.7 – -4.7 -3.2 -4.9 -4.4 -2.7 -5.3 -3.5 -1.0 -0.4 -0.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.0792 0.3073 0.0075 -0.6248 -0.6182 -0.0691 0.8309 0.9194 1.0264 1.0327 1.1901 0.8138 0.3466 0.5951 0.3789 0.4164 1.1229 0.3521
Actin 0.0236 0.0002 0.0032 0.0395 0.024 0.0029 0.0104 0.0002 0.017 0.001 0.0079 0.0024 0.0154 0.0014 0.0106 0.0002 0.0142 0.0066
Bud 0.0005 0.0001 0.0001 0.0001 0.0005 0.0001 0.0006 0.0001 0.0003 0.0016 0.0005 0.0003 0.0002 0.0002 0.0009 0 0.0002 0.0001
Bud Neck 0.001 0.0005 0.0005 0.001 0.0016 0.0028 0.003 0.0006 0.0009 0.0026 0.0013 0.0027 0.003 0.0004 0.0003 0.0005 0.0016 0.0027
Bud Periphery 0.0011 0.0001 0.0001 0.0001 0.0014 0.0002 0.0016 0.0001 0.0007 0.0049 0.0022 0.001 0.0004 0.0004 0.0002 0 0.0002 0.0002
Bud Site 0.0018 0.001 0.0006 0.0013 0.0007 0.0001 0.0026 0.0008 0.0061 0.0027 0.0004 0.0002 0.0009 0.0003 0.0005 0.0001 0.0004 0.0003
Cell Periphery 0.0003 0 0 0 0.0003 0 0.0003 0 0.0002 0.0008 0.0002 0 0.0002 0 0 0 0.0001 0
Cytoplasm 0.0153 0.0128 0.019 0.0015 0.0026 0.0081 0.0127 0.0136 0.013 0.0038 0.0119 0.0079 0.0075 0.0144 0.0063 0.0092 0.0242 0.0053
Cytoplasmic Foci 0.0265 0.0003 0.005 0.0049 0.0072 0.0021 0.0182 0.0008 0.0085 0.0025 0.0087 0.0015 0.0058 0.0002 0.0027 0.0001 0.0064 0.0012
Eisosomes 0.0004 0 0.0005 0.0001 0.0054 0 0.0002 0 0.0009 0 0.0002 0 0.0001 0 0 0 0.0002 0.0001
Endoplasmic Reticulum 0.0049 0.0068 0.0013 0.0001 0.0015 0.001 0.0024 0.0008 0.0013 0.0024 0.0042 0.0007 0.0022 0.001 0.0002 0.0004 0.0037 0.0006
Endosome 0.0431 0.003 0.0055 0.0005 0.0241 0.0026 0.0292 0.0013 0.0145 0.0187 0.0414 0.0044 0.0092 0.0005 0.0006 0.0005 0.0207 0.0038
Golgi 0.0086 0 0.0051 0.0017 0.016 0.0006 0.0092 0.0001 0.0041 0.0033 0.0191 0.0042 0.0014 0.0001 0.0005 0 0.0053 0.0016
Lipid Particles 0.0417 0.0001 0.0046 0.0022 0.089 0.0059 0.0301 0.0001 0.0102 0.0096 0.0296 0.0032 0.0045 0 0.0031 0 0.0149 0.0018
Mitochondria 0.0216 0.0003 0.0022 0.0006 0.0813 0.001 0.0112 0.0003 0.0271 0.0115 0.0486 0.0035 0.0017 0.0015 0.0004 0.0002 0.0021 0.0017
None 0.017 0.0155 0.0331 0.0002 0.0494 0.0248 0.0155 0.0242 0.0086 0.0013 0.0237 0.0128 0.0043 0.0049 0.0137 0.0477 0.0613 0.0033
Nuclear Periphery 0.0304 0.0377 0.0225 0.0087 0.0305 0.0158 0.0171 0.0119 0.0177 0.0095 0.0187 0.006 0.0245 0.0103 0.0103 0.0102 0.0267 0.0069
Nucleolus 0.0074 0.0144 0.01 0.0043 0.0363 0.0138 0.0111 0.012 0.0096 0.014 0.0135 0.0257 0.0102 0.0114 0.0108 0.0087 0.0133 0.0173
Nucleus 0.6866 0.8651 0.8485 0.8831 0.5374 0.8757 0.783 0.8988 0.8227 0.8596 0.6763 0.8918 0.8659 0.9237 0.9105 0.9118 0.7699 0.9116
Peroxisomes 0.0284 0 0.002 0.0151 0.0119 0.0018 0.0174 0.0001 0.0071 0.0019 0.0588 0.0008 0.0085 0.0002 0.0051 0 0.0048 0.0008
Punctate Nuclear 0.028 0.0397 0.035 0.0349 0.0721 0.0397 0.0193 0.033 0.0271 0.0236 0.028 0.0301 0.0313 0.0285 0.023 0.0104 0.0267 0.0337
Vacuole 0.0084 0.0017 0.0008 0 0.0039 0.0006 0.0035 0.0008 0.0015 0.0201 0.0021 0.0005 0.0019 0.0004 0.0002 0.0001 0.0019 0.0004
Vacuole Periphery 0.0033 0.0006 0.0005 0.0001 0.003 0.0003 0.0014 0.0003 0.0008 0.0045 0.0027 0.0004 0.0009 0.0002 0.0001 0.0001 0.0011 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.4759 4.6164 8.3737 11.7019 10.7872 12.043 12.5768 16.2768 10.5976 12.8429
Translational Efficiency 0.7447 1.4095 0.679 0.5333 0.5697 0.6684 0.5239 0.3537 0.6803 0.6768

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Histone H3 acetylase with a role in transcriptional regulation; sequence-specific activator of histone genes, binds specifically and cooperatively to pairs of UAS elements in core histone promoters, functions at or near TATA box; involved in S phase-specific acetylation of H3K56 at histone promoters, which is required for recruitment of SWI/SNF nucleosome remodeling complex and subsequent transcription
Localization
Cell Percentages nucleus (72%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Spt10

Spt10


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Spt10-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available