Standard name
Human Ortholog
Description MAP kinase kinase of the HOG signaling pathway; activated under severe osmotic stress; mitophagy-specific regulator; plays a role in regulating Ty1 transposition
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.05 0 0.05 0.06 0 – 0 0.06 0 0.09 0 0 0 0.14 0.11 0.08 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.95 0.97 0.94 0.88 0.82 – 0.74 0.73 0.67 0.67 0.99 0.99 0.99 0.89 0.87 0.91 0.98 0.96 0.9 0.93 0.94 0.95
Endoplasmic Reticulum 0 0 0 0 0 0 0.06 – 0.05 0 0.05 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.1 0.13 – 0.2 0.23 0.4 0.28 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.05 0 – 0 0.06 0.05 0.06 0 0 0 0.06 0.12 0.08 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 2 0 0 2 5 – 4 6 4 9 1 1 0 1 1 0 0 0 1 0 0 0
Bud 0 1 0 0 0 3 1 – 0 6 6 7 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 6 9 3 15 17 13 – 9 24 10 32 11 8 14 57 39 22 0 0 0 0 0 0
Cytoplasm 179 163 176 104 260 251 308 – 175 274 190 226 289 332 380 369 315 256 176 163 172 141 217 285
Endoplasmic Reticulum 0 3 5 1 6 4 22 – 12 5 13 12 2 0 3 15 17 11 0 1 4 0 3 2
Endosome 0 0 0 1 0 0 1 – 0 2 0 0 0 0 0 0 0 2 0 1 1 1 1 3
Golgi 0 0 1 0 0 0 1 – 0 0 0 0 0 1 0 1 2 0 0 0 0 0 1 1
Mitochondria 1 0 0 1 2 28 49 – 47 86 114 94 1 1 0 3 0 3 0 1 3 0 1 3
Nucleus 0 1 5 2 2 0 2 – 4 3 2 2 1 1 0 7 5 5 0 0 0 1 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 1 0 0 0 – 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 1 1 9 14 10 – 7 21 15 20 1 2 1 23 45 22 0 1 4 5 2 1
Unique Cell Count 180 167 186 107 276 284 377 237 373 282 339 293 337 385 413 362 282 180 171 191 152 232 301
Labelled Cell Count 181 174 199 114 294 319 412 259 428 354 404 306 347 398 476 424 321 180 171 191 152 232 301


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.6 13.3 10.9 9.6 10.8 9.1 9.0 – 7.6 7.8 6.8 7.5 11.5 11.9 11.6 11.9 11.8 11.3 9.1 10.3 10.8
Std Deviation (1e-4) 1.1 1.6 1.6 1.5 1.5 1.6 1.6 – 1.7 1.4 1.2 1.3 1.4 1.7 1.5 1.9 1.6 2.1 1.2 1.3 1.6
Intensity Change (Log2) – – – -0.18 -0.01 -0.26 -0.28 – -0.52 -0.48 -0.69 -0.55 0.08 0.12 0.09 0.12 0.11 0.05 -0.26 -0.08 -0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.3 0.5 -0.8 – -0.5 0.8 -0.7 1.9 -0.6 -1.5 -0.7 3.2 2.3 1.3
Cytoplasm 1.0 -0.2 -2.3 -4.2 – -5.6 -6.0 -7.0 -7.2 2.5 2.6 2.9 -2.1 -2.8 -1.5
Endoplasmic Reticulum 0 0 0 1.6 – 1.2 0 1.1 0.5 0 0 0 0.6 1.1 0.7
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.4 5.1 – 6.4 7.1 10.0 7.9 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 2.6 0 – 0 2.9 2.8 3.0 0 0 0 2.9 4.8 3.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.6434 6.4364 5.0308 3.906 3.5762 5.5126 5.7099 8.4404 7.6343 7.1457 6.4861 7.2929 5.5715 7.9237 7.4019 7.2013 6.8919 7.1955
Actin 0.0349 0.0068 0.0624 0.0068 0.0263 0.0014 0.0209 0.0004 0.0111 0.0002 0.0162 0.0171 0.0296 0.0006 0.006 0.0001 0.0004 0.0009
Bud 0.0017 0.0003 0.001 0.0013 0.0008 0.004 0.0006 0.0002 0.0003 0.0005 0.0002 0.0044 0.0007 0.0003 0.0007 0.0002 0.0007 0.0002
Bud Neck 0.0018 0.0003 0.0024 0.0007 0.0012 0.0011 0.002 0.0001 0.0003 0.0003 0.0006 0.0011 0.0024 0.0002 0.0003 0.0004 0.0008 0.0014
Bud Periphery 0.0055 0.0001 0.001 0.001 0.0008 0.002 0.0005 0.0001 0.0002 0.0001 0.0001 0.0035 0.0009 0.0001 0.0005 0.0001 0.0005 0.0002
Bud Site 0.0055 0.0015 0.0044 0.0081 0.002 0.0003 0.0046 0.0009 0.0006 0.0002 0.0005 0.0062 0.0055 0.0006 0.0009 0.0001 0.0007 0.0002
Cell Periphery 0.0022 0.0003 0.0005 0.0006 0.0002 0.0002 0.0006 0.0003 0.0002 0.0001 0.0001 0.0004 0.0005 0.0001 0.0001 0 0.0001 0.0001
Cytoplasm 0.7416 0.9552 0.8177 0.7026 0.6104 0.8569 0.7473 0.9645 0.9033 0.9275 0.8539 0.8754 0.7455 0.9518 0.9163 0.8909 0.8976 0.9077
Cytoplasmic Foci 0.0358 0.0106 0.0131 0.0261 0.0285 0.0345 0.0318 0.0062 0.009 0.0116 0.0425 0.0122 0.0259 0.007 0.0219 0.0137 0.0126 0.0161
Eisosomes 0.0004 0.0001 0.0003 0.0001 0.0003 0 0.0004 0 0.0002 0 0.0001 0.0001 0.0002 0 0 0 0 0
Endoplasmic Reticulum 0.0241 0.0027 0.0116 0.0333 0.02 0.009 0.0647 0.0091 0.0208 0.0072 0.0069 0.0209 0.0359 0.0126 0.0071 0.0029 0.0298 0.0172
Endosome 0.0595 0.0106 0.0388 0.1049 0.0404 0.0542 0.049 0.01 0.0231 0.0326 0.0336 0.0225 0.0551 0.0117 0.0247 0.066 0.0353 0.0287
Golgi 0.0249 0.0025 0.0173 0.0158 0.0431 0.0072 0.0136 0.0011 0.003 0.0011 0.0137 0.0041 0.0145 0.0013 0.0028 0.0009 0.0025 0.0053
Lipid Particles 0.0123 0.0021 0.001 0.0047 0.0298 0.004 0.0078 0.0002 0.0024 0.0001 0.0072 0.0031 0.0121 0.0041 0.0012 0 0.0003 0.003
Mitochondria 0.0214 0.0003 0.0112 0.0516 0.1381 0.0155 0.0063 0.0003 0.0008 0.0005 0.0012 0.0032 0.0146 0.002 0.0019 0.0002 0.0016 0.0071
None 0.0038 0.0011 0.0021 0.0038 0.0016 0.0007 0.0102 0.0012 0.0086 0.0013 0.0122 0.0037 0.0067 0.0011 0.0018 0.0008 0.0022 0.0017
Nuclear Periphery 0.0024 0.0003 0.0012 0.0072 0.0018 0.0012 0.0067 0.0007 0.0028 0.0016 0.001 0.0016 0.0093 0.0009 0.001 0.0008 0.0021 0.0013
Nucleolus 0.0002 0 0.0001 0.0003 0.0002 0.0002 0.0014 0 0.0001 0 0 0.0001 0.0007 0.0002 0.0001 0 0.0001 0.0003
Nucleus 0.0043 0.0004 0.0009 0.0029 0.0007 0.0008 0.0023 0.0006 0.0009 0.0014 0.0006 0.001 0.0035 0.0017 0.0011 0.0008 0.0032 0.0011
Peroxisomes 0.0083 0.0011 0.0014 0.0012 0.0438 0.0019 0.0085 0 0.0017 0.0001 0.006 0.0024 0.0104 0.0006 0.0051 0 0.0001 0.0008
Punctate Nuclear 0.001 0.0002 0.0002 0.0019 0.0053 0.0003 0.0098 0.0001 0.0016 0.0001 0.0014 0.013 0.0115 0.0003 0.0011 0.0001 0.0005 0.0027
Vacuole 0.0071 0.003 0.0101 0.0198 0.0028 0.0028 0.0096 0.0033 0.0078 0.0114 0.0016 0.0028 0.0119 0.0022 0.0045 0.0184 0.0073 0.003
Vacuole Periphery 0.0013 0.0004 0.0014 0.0053 0.0018 0.0017 0.0015 0.0004 0.0014 0.002 0.0004 0.0013 0.0027 0.0006 0.0009 0.0036 0.0016 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 30.7876 18.1843 20.058 34.9947 20.3151 21.9789 31.5546 34.3774 29.4994 38.0269
Translational Efficiency 1.3141 1.5574 1.4445 0.8323 1.5703 2.0845 0.8504 0.8664 1.0547 1.0529

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description MAP kinase kinase of the HOG signaling pathway; activated under severe osmotic stress; mitophagy-specific regulator; plays a role in regulating Ty1 transposition
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-4

Pbs2

Pbs2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pbs2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available