Standard name
Human Ortholog
Description Iron transporter, mediates Fe2+ transport across inner mito membrane; mitochondrial carrier family member; active under low-iron conditions; may transport other cations; MRS3 has a paralog, MRS4, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 0 – 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0
Bud – 0 0 0 0 0.05 0 0 – 0 0 0 0 0 0 0 0 0 0.11 0 0 0 0 0
Bud Neck – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 0.4 0.35 0.32 0.25 0.21 0.2 0.12 – 0.11 0.07 0.11 0.41 0.46 0.52 0.81 0.82 0.8 0 0.08 0.05 0 0 0
Endoplasmic Reticulum – 0 0 0 0 0.06 0 0 – 0 0 0 0.07 0 0 0.05 0.09 0.05 0 0 0 0 0 0
Endosome – 0.21 0.06 0 0.08 0 0 0 – 0 0 0 0.16 0.2 0.23 0.07 0.06 0.05 0 0.13 0.09 0.1 0.09 0.08
Golgi – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria – 0.28 0.52 0.71 0.68 0.79 0.83 0.91 – 0.93 0.95 0.95 0.11 0.11 0 0.06 0 0 0.36 0.51 0.66 0.62 0.59 0.54
Nucleus – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0.07 0 0.05 0 0.06 0.07 – 0.05 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 0.2 0.07 0.06 0.06 0 0 0 – 0 0 0 0.18 0.24 0.2 0.05 0.06 0.07 0.39 0.09 0 0.09 0.11 0.12
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 2 1 0 0 2 0 0 – 0 0 0 21 6 1 0 0 0 0 33 8 14 24 29
Bud – 10 8 2 1 9 7 11 – 9 2 4 7 2 4 6 0 1 0 20 3 5 12 14
Bud Neck – 0 1 0 1 1 0 1 – 1 0 0 2 3 0 4 3 0 0 14 1 13 18 28
Bud Site – 0 0 0 0 2 4 2 – 1 1 0 0 0 0 0 0 1 – – – – – –
Cell Periphery – 13 4 0 0 0 0 0 – 1 0 0 13 7 10 10 8 6 0 5 1 2 2 4
Cytoplasm – 267 67 22 28 42 47 37 – 38 16 25 137 209 224 304 249 221 0 69 11 17 21 22
Endoplasmic Reticulum – 12 6 3 5 11 1 4 – 4 2 1 23 9 9 17 26 14 0 15 4 5 14 13
Endosome – 141 11 0 9 1 4 1 – 1 1 0 53 90 99 27 17 14 0 113 23 58 72 54
Golgi – 0 0 0 0 0 0 0 – 0 0 0 0 3 1 0 3 4 0 20 0 7 25 20
Mitochondria – 187 98 48 77 157 200 276 – 326 210 225 37 49 19 22 13 8 1 448 166 370 464 375
Nucleus – 2 0 0 2 3 3 5 – 2 2 7 5 4 1 2 1 1 0 3 0 3 2 2
Nuclear Periphery – 0 0 0 0 0 0 3 – 3 1 2 1 0 0 0 0 0 0 3 1 1 4 3
Nucleolus – 0 0 0 0 0 2 2 – 1 1 1 0 0 0 0 0 0 0 3 0 3 4 2
Peroxisomes – 14 2 0 2 0 0 0 – 1 0 0 2 9 9 1 8 5 0 3 4 9 8 12
SpindlePole – 21 14 2 6 7 14 21 – 16 4 4 17 13 16 13 6 7 0 15 10 17 15 14
Vac/Vac Membrane – 133 13 4 7 1 7 3 – 5 4 6 61 109 88 18 17 19 1 80 4 53 85 87
Unique Cell Count 669 190 68 114 198 240 304 351 221 237 334 458 434 375 304 277 3 877 253 598 791 698
Labelled Cell Count 802 225 81 138 236 289 366 409 244 275 379 513 481 424 351 301 3 877 253 598 791 698


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 5.0 4.1 3.6 3.6 3.3 2.8 2.9 – 3.0 3.0 3.0 6.0 5.9 5.9 5.7 5.6 5.9 6.3 6.5 6.4
Std Deviation (1e-4) – 0.9 0.8 0.4 1.4 0.9 0.7 0.7 – 0.5 0.8 0.8 0.8 0.8 0.9 1.2 1.4 1.4 1.0 1.2 1.2
Intensity Change (Log2) – – – -0.16 -0.17 -0.31 -0.51 -0.46 – -0.44 -0.46 -0.43 0.57 0.54 0.55 0.48 0.46 0.53 0.64 0.69 0.66


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 3.2 0 0 0 0 0
Bud 0 0 0 0 0 – 0 0 0 -1.4 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 1.1 0 0 0 0 0
Cytoplasm -0.4 -1.9 -3.1 -3.7 -6.1 – -6.9 -7.1 -6.2 1.3 2.4 3.8 10.8 10.5 9.7
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 1.8 0 0 0.8 2.4 1.0
Endosome 0 0 0 0 0 – 0 0 0 3.4 4.4 5.1 0.6 -0.1 -0.3
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 – 0 0 0 -10.2 -11.3 -13.9 -12.6 -12.3 -12.3
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 -1.1 -2.6 -2.0 -2.1 -3.0 -2.5
Vacuole 0 -0.2 -3.3 -1.9 -3.6 – -3.4 -2.6 -2.1 3.6 5.0 4.2 -1.0 -0.6 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.0748 0.8909 0.6168 0.4092 0.0989 0.5138 3.5266 3.5689 3.6862 4.0616 3.2459 3.956 1.2751 2.052 1.7886 1.5665 2.121 1.8908
Actin 0.0056 0.0064 0.0058 0.0018 0.0327 0.0099 0.0402 0.007 0.0184 0.0254 0.0076 0.0113 0.0059 0.0024 0.003 0.0027 0.0361 0.0052
Bud 0.0013 0.0137 0.004 0.0079 0.007 0.0016 0.0069 0.0089 0.0114 0.0096 0.0119 0.0058 0.0013 0.008 0.0041 0.0074 0.0079 0.0025
Bud Neck 0.0016 0.0008 0.0021 0.0008 0.0024 0.0052 0.0103 0.0021 0.0087 0.0042 0.0016 0.0051 0.0021 0.0017 0.0018 0.0014 0.0268 0.0055
Bud Periphery 0.0018 0.0077 0.0106 0.0136 0.0043 0.0031 0.0098 0.0121 0.0195 0.0169 0.0183 0.0092 0.0028 0.0105 0.008 0.0195 0.0405 0.0058
Bud Site 0.0064 0.027 0.0115 0.0019 0.0038 0.0014 0.0274 0.0637 0.0466 0.0151 0.0093 0.0048 0.0086 0.0526 0.0117 0.0017 0.0066 0.003
Cell Periphery 0.0013 0.0011 0.0017 0.0005 0.0006 0.0008 0.0023 0.002 0.0024 0.0009 0.0005 0.0009 0.0014 0.0016 0.0014 0.0006 0.0011 0.0009
Cytoplasm 0.1567 0.3049 0.2204 0.2975 0.167 0.2103 0.1677 0.2666 0.1786 0.2973 0.1785 0.216 0.2657 0.3683 0.2808 0.281 0.213 0.3488
Cytoplasmic Foci 0.1421 0.0863 0.1156 0.0985 0.1237 0.1364 0.1379 0.1076 0.1363 0.1139 0.1204 0.1616 0.1211 0.1172 0.1283 0.1306 0.1268 0.1287
Eisosomes 0.0009 0.0005 0.0014 0.0002 0.0003 0.0009 0.001 0.0004 0.0007 0.0004 0.0003 0.0002 0.0004 0.0001 0.0002 0.0001 0.0004 0.0002
Endoplasmic Reticulum 0.0096 0.011 0.0068 0.0093 0.0027 0.0059 0.0134 0.0058 0.0106 0.0114 0.0058 0.0046 0.0182 0.0077 0.0073 0.0141 0.0119 0.0142
Endosome 0.1642 0.1319 0.1249 0.1531 0.189 0.1449 0.1792 0.1239 0.1503 0.0972 0.2574 0.1388 0.2107 0.1592 0.1854 0.196 0.1653 0.1608
Golgi 0.0205 0.015 0.0207 0.0177 0.0203 0.0221 0.0436 0.0299 0.0445 0.019 0.0432 0.0316 0.0263 0.0292 0.0338 0.0216 0.031 0.0258
Lipid Particles 0.019 0.004 0.0066 0.0064 0.0158 0.01 0.0128 0.0037 0.0069 0.0107 0.0091 0.0058 0.0112 0.0031 0.0056 0.0031 0.0044 0.007
Mitochondria 0.0758 0.0276 0.124 0.0815 0.069 0.0974 0.0926 0.0758 0.1058 0.0406 0.0806 0.0915 0.0621 0.0446 0.1195 0.0994 0.0434 0.0818
None 0.3425 0.3192 0.299 0.2694 0.2683 0.3059 0.1977 0.2468 0.2054 0.2509 0.1895 0.2609 0.2047 0.15 0.172 0.1806 0.2447 0.1744
Nuclear Periphery 0.0045 0.0033 0.002 0.0039 0.002 0.0016 0.0054 0.0024 0.004 0.012 0.0036 0.0015 0.0119 0.0028 0.0032 0.0027 0.0046 0.0024
Nucleolus 0.0025 0.0014 0.0011 0.0008 0.0032 0.0009 0.0012 0.0021 0.0021 0.0027 0.0016 0.0009 0.0013 0.0014 0.0007 0.0005 0.0009 0.0013
Nucleus 0.0038 0.0051 0.0026 0.0032 0.0075 0.0023 0.0049 0.0062 0.0043 0.01 0.0067 0.0042 0.0063 0.0078 0.0029 0.0022 0.0039 0.0038
Peroxisomes 0.0102 0.0052 0.0165 0.0048 0.0358 0.0218 0.0191 0.0062 0.0206 0.0186 0.0181 0.0283 0.0063 0.0058 0.0087 0.0067 0.0122 0.0081
Punctate Nuclear 0.0071 0.006 0.002 0.0025 0.0063 0.0037 0.0052 0.0024 0.0044 0.0263 0.0079 0.0033 0.0036 0.0036 0.0016 0.0038 0.004 0.0028
Vacuole 0.0189 0.0185 0.0162 0.0158 0.0335 0.0102 0.0167 0.0208 0.0141 0.0125 0.0207 0.0101 0.0225 0.0187 0.0151 0.0159 0.0115 0.0128
Vacuole Periphery 0.0038 0.0034 0.0042 0.0089 0.005 0.004 0.0049 0.0036 0.0044 0.0044 0.0072 0.0037 0.0058 0.0037 0.005 0.0082 0.0029 0.0041

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 26.2715 9.3941 16.2652 26.9595 28.0067 14.0037 18.2051 24.2183 19.7063 28.1207
Translational Efficiency 0.6356 1.1023 0.9028 0.4445 0.454 1.0937 0.821 0.5698 0.6721 0.5455

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Iron transporter, mediates Fe2+ transport across inner mito membrane; mitochondrial carrier family member; active under low-iron conditions; may transport other cations; MRS3 has a paralog, MRS4, that arose from the whole genome duplication
Localization
Cell Percentages mitochondrion (42%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Mrs3

Mrs3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mrs3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available