Standard name
Human Ortholog
Description Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF2 has a paralog, TIF1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0.06 0.06 0.07 0.13 0.13 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.99 1.0 1.0 0.96 0.92 0.79 0.76 0.74 0.77 0.71 0.68 1.0 1.0 0.99 0.98 0.98 0.98 0.98 0.98 0.94 0.95 0.97 0.95
Endoplasmic Reticulum 0 0 0 0 0 0.06 0.18 0.19 0.2 0.13 0.16 0.2 0 0 0 0.06 0.06 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 2 4 7 7 9 22 14 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 1 0 0 0 0 7 0 1 2 1 3 0 0 0 0 0 1 1 0 1 0 2 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 2 4 4 6 8 14 23 21 23 51 46 0 10 5 5 5 7 0 0 1 0 0 0
Cytoplasm 259 326 402 272 290 374 401 277 261 261 270 245 189 410 422 429 320 259 260 338 386 199 233 268
Endoplasmic Reticulum 0 1 1 0 10 24 91 70 72 43 59 72 0 2 3 25 19 14 2 0 12 0 1 4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 1 1 2 4 1 4 3 0 0 0 0 1 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 3 5 1 2 8 6 6 0 0 0 0 0 0 0 0 1 0 0 0
Nucleus 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 1 0 0 0 0 1 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 1 0 0 0 1 2 3 0 0 1 2 7 1 0 0 1 4 1 0
Unique Cell Count 259 328 403 272 301 406 510 365 353 338 379 359 189 412 425 436 326 263 267 346 410 210 242 283
Labelled Cell Count 260 330 407 276 306 413 523 380 370 348 416 393 189 422 431 462 353 282 267 346 410 210 242 283


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 121.8 173.8 128.1 108.5 89.5 79.0 74.1 74.2 70.2 67.1 61.0 60.8 144.0 139.6 127.3 218.4 182.3 211.1 165.5 172.2 177.9
Std Deviation (1e-4) 20.9 32.2 26.4 27.6 21.6 20.0 17.9 16.2 18.3 16.3 15.2 14.9 32.9 27.3 26.3 76.0 63.5 75.4 46.4 41.6 46.0
Intensity Change (Log2) – – – -0.24 -0.52 -0.7 -0.79 -0.79 -0.87 -0.93 -1.07 -1.08 0.17 0.12 -0.01 0.77 0.51 0.72 0.37 0.43 0.47


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 4.9 4.0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 1.2 1.9 4.0 3.8 4.2 6.8 6.6 0 1.6 0 0 0 0
Cytoplasm 0.8 -3.5 -5.5 -9.7 -10.3 -10.8 -10.0 -11.5 -12.1 0.7 -0.6 -0.9 -2.0 -2.2 -1.9
Endoplasmic Reticulum 0 0 4.7 8.8 9.0 9.4 7.2 8.0 9.3 0 0 0 4.6 4.6 4.3
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 123.1085 158.5018 152.5323 150.1439 149.0027 156.8849 128.2078 190.4876 175.7217 150.3948 162.8587 162.6441 149.5423 171.9735 164.3583 157.2301 146.2816 152.5626
Actin 0.0124 0.0001 0.0013 0.0005 0.0005 0.0004 0.01 0.0002 0.0002 0.0344 0.0151 0.0019 0.0036 0.0002 0.003 0.0084 0.0002 0.0006
Bud 0.0015 0.0003 0.0005 0.0011 0.0008 0.0006 0.0019 0.0007 0.0009 0.0019 0.002 0.0024 0.0009 0.0004 0.0007 0.0011 0.001 0.0009
Bud Neck 0.0024 0.0004 0.001 0.0012 0.0036 0.0025 0.0041 0.0006 0.0012 0.0019 0.0019 0.0063 0.0009 0.0005 0.0008 0.0016 0.0009 0.0024
Bud Periphery 0.0017 0.0003 0.0003 0.0015 0.0007 0.0006 0.0031 0.0005 0.0008 0.0026 0.0013 0.0026 0.0021 0.0005 0.0008 0.001 0.0014 0.0008
Bud Site 0.0027 0.0003 0.0004 0.0002 0.0002 0.0001 0.0058 0.0008 0.0005 0.0006 0.0012 0.0007 0.0004 0.0003 0.0003 0.0009 0.0008 0.0002
Cell Periphery 0.0461 0.0311 0.0299 0.0254 0.0057 0.0087 0.0873 0.0714 0.0686 0.0469 0.0543 0.0138 0.0404 0.0296 0.0266 0.0361 0.0056 0.0083
Cytoplasm 0.6137 0.8826 0.8263 0.721 0.8063 0.854 0.5452 0.7765 0.6846 0.6456 0.5367 0.8093 0.6951 0.8851 0.8261 0.6257 0.7573 0.8298
Cytoplasmic Foci 0.0104 0.0005 0.0039 0.0018 0.002 0.002 0.0114 0.0004 0.005 0.0011 0.0027 0.005 0.0076 0.0005 0.0026 0.0055 0.0048 0.0032
Eisosomes 0.0008 0 0.0001 0 0 0 0.0026 0 0.0001 0.0004 0.0006 0.0001 0.0003 0.0002 0.0002 0.0004 0.0001 0.0001
Endoplasmic Reticulum 0.0456 0.0083 0.0174 0.0126 0.0232 0.0303 0.0265 0.0052 0.0136 0.0145 0.0516 0.0297 0.0585 0.0136 0.0262 0.0414 0.0115 0.0643
Endosome 0.0091 0.001 0.0026 0.0107 0.0069 0.0079 0.0055 0.0006 0.0012 0.0037 0.0073 0.004 0.0029 0.0008 0.0021 0.0093 0.0112 0.0054
Golgi 0.0037 0.0001 0.0004 0.0009 0.0007 0.0012 0.0016 0.0001 0.0001 0.0018 0.0025 0.0007 0.0006 0.0001 0.0004 0.0023 0.0009 0.0009
Lipid Particles 0.0102 0.0019 0.0028 0.0047 0.0016 0.0021 0.013 0.0022 0.0053 0.0027 0.0156 0.0032 0.005 0.0021 0.0026 0.0065 0.0026 0.0021
Mitochondria 0.0103 0.0001 0.0009 0.0006 0.0015 0.001 0.0083 0.0002 0.0038 0.0015 0.0111 0.0011 0.0061 0.0004 0.0021 0.0018 0.004 0.0021
None 0.0054 0.0002 0.002 0.0003 0.0002 0.0004 0.0212 0.0002 0.0011 0.0007 0.0025 0.0014 0.0035 0.0002 0.0036 0.0049 0.0082 0.0008
Nuclear Periphery 0.022 0.0031 0.0075 0.0074 0.0097 0.0069 0.0296 0.0049 0.0096 0.0105 0.0166 0.009 0.0169 0.0043 0.0076 0.0105 0.0059 0.0099
Nucleolus 0.0124 0.0002 0.0023 0.0004 0.0001 0.0006 0.0283 0.0007 0.0096 0.0011 0.0038 0.0036 0.0093 0.0003 0.002 0.0158 0.0119 0.0067
Nucleus 0.0119 0.0014 0.0037 0.0034 0.0023 0.0031 0.0199 0.0038 0.0077 0.007 0.0133 0.0183 0.0095 0.002 0.0048 0.0083 0.0241 0.0047
Peroxisomes 0.0056 0 0.0002 0 0.0001 0.0001 0.0016 0 0.0003 0.0002 0.0037 0.0002 0.0006 0 0.0002 0.0009 0.0005 0.0001
Punctate Nuclear 0.0101 0 0.0018 0.0001 0.0001 0.0001 0.0023 0 0.0002 0.0004 0.0078 0.0005 0.0011 0 0.0003 0.0049 0.0005 0.0001
Vacuole 0.1394 0.0654 0.0878 0.1787 0.1136 0.0586 0.153 0.1272 0.176 0.2015 0.2257 0.0712 0.1213 0.0567 0.0824 0.1876 0.1194 0.0492
Vacuole Periphery 0.0225 0.0025 0.0066 0.0276 0.0201 0.0188 0.0177 0.0038 0.0096 0.0189 0.0226 0.015 0.0136 0.0022 0.0047 0.025 0.0271 0.0074

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1812.0724 1780.957 1784.8585 1547.7143 1408.334 1585.4979 1587.4912 1360.9846 1312.8922 1368.7419
Translational Efficiency 0.8043 0.8589 0.7891 0.8695 0.9189 0.8969 0.8699 0.9218 0.8477 0.8325

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF2 has a paralog, TIF1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-1

Tif2

Tif2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tif2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available