Standard name | |
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Human Ortholog | |
Description | RNA polymerase II subunit B32; forms dissociable heterodimer with Rpb7p; Rpb4/7 dissociates from RNAPII as Ser2 CTD phosphorylation increases; Rpb4/7 regulates cellular lifespan via mRNA decay process; involved in recruitment of 3'-end processing factors to transcribing RNAPII complex, export of mRNA to cytoplasm under stress conditions; also involved in translation initiation |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 9 | 5 | 4 | 3 |
Bud Neck | 0 | 1 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | – | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 1 | 2 | 0 | 1 | 2 | 2 | 1 | 1 | – | – | 2 | 3 | 1 | 1 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 2 | 10 | 8 | 4 | 4 | 6 | 17 | 10 | 4 | – | – | 7 | 12 | 22 | 15 | 9 | 24 | 0 | 1 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | – | 0 | 0 | 0 | 3 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 2 | 1 | 2 | 0 | 3 | 2 | 1 | 0 | – | – | 0 | 3 | 1 | 4 | 2 | 2 | 1 | 2 | 4 | 1 | 1 | 0 |
Golgi | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 3 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 |
Mitochondria | 2 | 3 | 3 | 1 | 0 | 22 | 2 | 5 | 2 | 7 | – | – | 1 | 2 | 1 | 12 | 10 | 13 | 3 | 0 | 4 | 2 | 6 | 4 |
Nucleus | 9 | 93 | 112 | 55 | 94 | 79 | 112 | 147 | 38 | 77 | – | – | 113 | 107 | 69 | 84 | 77 | 101 | 27 | 90 | 103 | 31 | 77 | 53 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 3 | – | – | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 1 | 1 |
Nucleolus | 12 | 80 | 84 | 29 | 45 | 78 | 68 | 98 | 20 | 65 | – | – | 91 | 107 | 83 | 69 | 55 | 58 | 13 | 28 | 68 | 51 | 113 | 78 |
Peroxisomes | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | – | – | 0 | 3 | 4 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 10 | 3 | 2 | 1 | 2 | 4 | 2 | 1 | – | – | 3 | 4 | 5 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 5 | 3 |
Vac/Vac Membrane | 0 | 1 | 6 | 4 | 5 | 3 | 10 | 5 | 1 | 0 | – | – | 2 | 3 | 0 | 2 | 7 | 9 | 2 | 6 | 10 | 7 | 13 | 13 |
Unique Cell Count | 16 | 121 | 179 | 96 | 137 | 147 | 180 | 246 | 61 | 128 | 157 | 192 | 153 | 151 | 132 | 175 | 55 | 136 | 220 | 103 | 232 | 168 | ||
Labelled Cell Count | 25 | 180 | 232 | 104 | 153 | 189 | 206 | 286 | 77 | 160 | 220 | 247 | 186 | 191 | 161 | 208 | 55 | 136 | 220 | 103 | 232 | 168 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 10.3 | 15.0 | 17.9 | 17.5 | 17.2 | 11.0 | 15.5 | 15.5 | 13.4 | 14.0 | – | – | 19.0 | 18.8 | 17.5 | 16.4 | 20.4 | 18.3 | 14.7 | 16.3 | 17.3 |
Std Deviation (1e-4) | 6.1 | 5.4 | 6.6 | 6.7 | 5.8 | 4.5 | 5.7 | 5.4 | 4.8 | 4.9 | – | – | 6.7 | 7.5 | 7.4 | 6.9 | 9.3 | 8.6 | 7.5 | 5.2 | 5.1 |
Intensity Change (Log2) | – | – | – | -0.04 | -0.06 | -0.7 | -0.21 | -0.21 | -0.42 | -0.36 | – | – | 0.08 | 0.07 | -0.04 | -0.13 | 0.18 | 0.02 | -0.29 | -0.13 | -0.06 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.9 | -1.1 | -1.3 | -1.0 | 0.6 | 2.6 | -1.0 | – | – | -0.5 | 0.3 | 2.7 | 1.5 | 0.4 | 2.6 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 4.5 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 2.7 | 2.6 | 2.6 |
Nucleus | -0.9 | 1.1 | -1.6 | -0.1 | -0.6 | 0 | -0.4 | – | – | 1.8 | -1.3 | -3.2 | -1.3 | -0.8 | -0.9 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -2.7 | -2.5 | 1.1 | -1.8 | -1.5 | -1.9 | 0.7 | – | – | 2.0 | 1.7 | 1.3 | -0.2 | -0.9 | -2.6 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | -1.9 | 0 | -2.4 | -2.3 | 0 | 0 | – | – | -1.7 | -1.8 | -1.0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 1.0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0.8 | 0.8 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 8.1496 | 11.3762 | 9.7317 | 9.1959 | 9.1844 | 9.1274 | 12.631 | 15.7464 | 14.2986 | 12.4989 | 9.7648 | 13.8741 | 16.6765 | 20.0416 | 18.0439 | 17.123 | 17.9384 | 17.6855 |
Actin | 0.0494 | 0.0012 | 0.0238 | 0.002 | 0.0081 | 0.0035 | 0.044 | 0 | 0.0076 | 0.0011 | 0.0383 | 0.0002 | 0.02 | 0 | 0 | 0 | 0.0001 | 0 |
Bud | 0.002 | 0 | 0.0013 | 0.0002 | 0.0002 | 0.0002 | 0.0017 | 0.0001 | 0.0005 | 0.0023 | 0.0031 | 0.0001 | 0.0003 | 0 | 0 | 0 | 0.0001 | 0 |
Bud Neck | 0.0011 | 0 | 0.0012 | 0.0001 | 0.0004 | 0.0005 | 0.0019 | 0 | 0.0038 | 0.0004 | 0.0019 | 0.0002 | 0.0004 | 0 | 0 | 0 | 0.0008 | 0.0002 |
Bud Periphery | 0.0031 | 0 | 0.0018 | 0.0003 | 0.0005 | 0.0006 | 0.0023 | 0.0003 | 0.0003 | 0.0013 | 0.003 | 0.0001 | 0.0004 | 0 | 0 | 0 | 0.0005 | 0 |
Bud Site | 0.0042 | 0 | 0.0024 | 0.0004 | 0.0007 | 0.0002 | 0.0114 | 0.0001 | 0.011 | 0.0041 | 0.0059 | 0.0001 | 0.0003 | 0 | 0 | 0 | 0.0002 | 0.0001 |
Cell Periphery | 0.0015 | 0 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0011 | 0.0001 | 0.0002 | 0.0001 | 0.0004 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0.0013 | 0.0001 |
Cytoplasm | 0.0289 | 0.0001 | 0.001 | 0.001 | 0.0026 | 0.0001 | 0.0094 | 0 | 0.0119 | 0.0099 | 0.0117 | 0 | 0.0047 | 0 | 0 | 0 | 0 | 0.0002 |
Cytoplasmic Foci | 0.0386 | 0.0002 | 0.0032 | 0.0017 | 0.0288 | 0.0006 | 0.0215 | 0.0024 | 0.0036 | 0.0006 | 0.0606 | 0.0011 | 0.0048 | 0 | 0 | 0 | 0 | 0.0019 |
Eisosomes | 0.0008 | 0 | 0.0002 | 0.0001 | 0.0005 | 0.0001 | 0.0004 | 0 | 0 | 0 | 0.0004 | 0 | 0.0003 | 0 | 0 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0067 | 0.0003 | 0.0009 | 0.0008 | 0.0009 | 0.0003 | 0.0059 | 0 | 0.0002 | 0.0007 | 0.0051 | 0 | 0.0025 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0.0338 | 0.0017 | 0.0087 | 0.001 | 0.0075 | 0.0022 | 0.0274 | 0.0001 | 0.0005 | 0.0005 | 0.0813 | 0.0004 | 0.012 | 0 | 0 | 0 | 0.0001 | 0.0001 |
Golgi | 0.01 | 0.0004 | 0.0035 | 0.0003 | 0.002 | 0.0012 | 0.0078 | 0 | 0.001 | 0.0001 | 0.0403 | 0.0008 | 0.0021 | 0 | 0 | 0 | 0 | 0 |
Lipid Particles | 0.0315 | 0.0003 | 0.0023 | 0.0095 | 0.0109 | 0.0026 | 0.0191 | 0.0029 | 0.0013 | 0.0001 | 0.1193 | 0.0008 | 0.0032 | 0 | 0 | 0 | 0.0001 | 0.001 |
Mitochondria | 0.0219 | 0.0005 | 0.0194 | 0.0514 | 0.0627 | 0.0144 | 0.0129 | 0.0059 | 0.0004 | 0.0027 | 0.0379 | 0.0191 | 0.006 | 0.0001 | 0.0002 | 0.0004 | 0.0113 | 0.0002 |
None | 0.0203 | 0.0001 | 0.0006 | 0.0006 | 0.0048 | 0.0003 | 0.0098 | 0 | 0.0001 | 0.0006 | 0.0026 | 0.0001 | 0.0217 | 0 | 0 | 0 | 0 | 0.0001 |
Nuclear Periphery | 0.0551 | 0.0047 | 0.0072 | 0.024 | 0.026 | 0.0176 | 0.0456 | 0.0008 | 0.0005 | 0.0169 | 0.0169 | 0.0001 | 0.0517 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0006 |
Nucleolus | 0.1904 | 0.0842 | 0.1077 | 0.11 | 0.1378 | 0.2566 | 0.1126 | 0.0768 | 0.1039 | 0.1611 | 0.1813 | 0.304 | 0.1663 | 0.0748 | 0.0769 | 0.0519 | 0.5321 | 0.2929 |
Nucleus | 0.4283 | 0.9048 | 0.7876 | 0.7857 | 0.649 | 0.6667 | 0.6079 | 0.9017 | 0.8475 | 0.7915 | 0.3372 | 0.6714 | 0.6938 | 0.925 | 0.9224 | 0.9475 | 0.4499 | 0.694 |
Peroxisomes | 0.0082 | 0 | 0.0017 | 0.0011 | 0.0014 | 0.0008 | 0.0089 | 0.0006 | 0.0034 | 0.0005 | 0.0293 | 0.0001 | 0.0014 | 0 | 0 | 0 | 0.0001 | 0 |
Punctate Nuclear | 0.0476 | 0.001 | 0.0221 | 0.0049 | 0.051 | 0.0302 | 0.038 | 0.0001 | 0.0017 | 0.004 | 0.0111 | 0.0005 | 0.0042 | 0 | 0.0001 | 0 | 0.0001 | 0.0085 |
Vacuole | 0.0082 | 0.0002 | 0.0015 | 0.0009 | 0.001 | 0.0004 | 0.0057 | 0.0025 | 0.0004 | 0.001 | 0.0073 | 0.0003 | 0.001 | 0 | 0 | 0 | 0.0016 | 0.0001 |
Vacuole Periphery | 0.0084 | 0.0003 | 0.0017 | 0.0038 | 0.0031 | 0.0009 | 0.0047 | 0.0054 | 0.0002 | 0.0005 | 0.0051 | 0.0004 | 0.0029 | 0 | 0 | 0 | 0.0015 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 59.6435 | 54.7987 | 45.059 | 62.664 | 79.4784 | 44.7078 | 51.6632 | 71.7597 | 72.8057 | 61.6573 |
Translational Efficiency | 2.0405 | 2.2367 | 2.3391 | 2.4557 | 1.4512 | 2.3717 | 2.0444 | 1.66 | 1.6866 | 1.7223 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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