Standard name
Human Ortholog
Description Ub-ligase substrate-specificity factor; proteolytically-cleaved form acts as a pseudosubstrate, binding and altering the substrate specificity of Ubr1p towards misfolded and native ER membrane and cytosolic proteins, as part of the stress-induced homeostatically regulated protein degradation (SHRED) pathway; hydrophilin essential during desiccation-rehydration; induced by osmotic stress, starvation and during stationary phase; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.97 0.97 0.92 0.86 – 0.62 0.65 0.62 0.58 0.54 0.98 0.98 0.99 0.86 0.77 0.87 0.95 0.92 0.89 0.95 0.93 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.06 0.26 0.11 0.27 – 0.31 0.45 0.47 0.55 0.59 0 0 0 0 0.06 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0.15 0.09 0.09 0.07 0 0 0 0 0.07 0.16 0.09 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 2 0 – 1 0 6 3 2 0 0 0 3 1 1 2 1 1 0 0 0
Bud 3 0 4 5 2 1 – 6 5 8 13 11 0 0 1 1 1 0 1 1 0 0 1 4
Bud Neck 1 0 1 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 3 1 1 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 1 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 10 4 1 1 6 8 – 19 11 26 25 22 0 1 0 5 1 3 0 0 1 0 0 0
Cytoplasm 610 382 201 311 489 387 – 398 236 351 256 371 84 175 159 116 122 129 598 373 176 73 131 128
Endoplasmic Reticulum 3 1 2 0 12 4 – 4 6 12 3 17 0 0 0 3 8 6 0 1 2 0 0 0
Endosome 0 2 0 0 0 1 – 3 0 2 0 0 0 1 0 4 12 4 1 2 0 0 0 2
Golgi 1 0 0 0 0 0 – 0 0 0 0 0 0 0 0 2 2 1 3 4 0 0 0 0
Mitochondria 8 2 12 85 56 121 – 198 163 267 245 402 3 2 1 5 9 1 6 8 8 3 3 0
Nucleus 0 1 2 0 2 1 – 9 1 1 2 3 1 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 1 0 0 0 0 0 – 12 3 3 4 10 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 2 1 0 0 0 0 0 0 3 11 3 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 0 0 0 – 0 0 1 0 0 0 0 0 2 6 1 1 0 0 0 0 2
Vac/Vac Membrane 0 2 0 0 8 9 – 96 32 52 29 27 0 0 1 10 25 14 3 4 0 0 1 1
Unique Cell Count 619 389 207 322 530 452 638 363 570 442 683 86 178 160 135 159 148 629 404 199 77 141 144
Labelled Cell Count 638 394 223 402 577 532 748 458 730 581 865 88 179 162 154 198 163 629 404 199 77 141 144


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.3 5.0 3.5 4.1 7.1 6.9 – 6.6 6.0 6.2 5.6 5.4 5.0 5.7 5.7 8.6 9.3 8.4 4.6 5.2 5.7
Std Deviation (1e-4) 0.6 1.0 1.0 1.1 1.5 1.5 – 1.7 1.8 2.1 2.4 1.9 1.0 1.1 1.1 4.3 4.2 3.8 0.8 1.3 1.5
Intensity Change (Log2) – – – 0.24 1.03 1.0 – 0.94 0.78 0.84 0.68 0.63 0.52 0.73 0.72 1.32 1.42 1.28 0.39 0.57 0.72


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0.7 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 2.7 3.1 2.2 0 0 0 0 0 0
Cytoplasm -0.3 -2.4 -4.4 – -9.6 -8.7 -9.7 -10.2 -11.3 0.3 0.8 1.6 -3.9 -6.0 -3.6
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 4.0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 2.0 6.2 – 7.3 9.7 10.5 12.0 13.4 0 -2.4 -2.7 -0.9 -0.1 -2.5
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 5.9 4.4 4.5 3.8 2.9 0 0 0 0 5.9 4.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.5969 1.5754 0.961 0.9132 0.7666 1.0945 -0.3929 0.3872 0.1532 0.6008 -0.282 0.3192 0.9829 2.396 1.888 1.8201 1.954 1.9276
Actin 0.0006 0.0006 0.0003 0.0001 0.0003 0.0013 0.0176 0.0045 0.011 0.0027 0.0021 0.0107 0.0119 0.0004 0.0004 0.0001 0.0001 0.0013
Bud 0.0036 0.001 0.0004 0.0005 0.0002 0.0002 0.0029 0.0027 0.0032 0.0007 0.0006 0.001 0.001 0.0071 0.0014 0.0002 0.0001 0.0001
Bud Neck 0.0003 0.0004 0.0002 0.0001 0.0002 0.0012 0.0143 0.0002 0.0008 0.0205 0.0001 0.0061 0.003 0.0001 0.0001 0.0001 0.0002 0.0026
Bud Periphery 0.0021 0.0006 0.0004 0.0008 0.0002 0.0001 0.007 0.0067 0.0037 0.0007 0.0008 0.0024 0.0014 0.0016 0.0004 0.0001 0.0001 0.0001
Bud Site 0.0027 0.0084 0.0006 0.0003 0.0001 0.0002 0.0091 0.012 0.0093 0.0073 0.0007 0.0004 0.0067 0.0026 0.0013 0.0001 0.0001 0.0001
Cell Periphery 0.0003 0.0002 0.0001 0.0001 0 0 0.0007 0.0004 0.0003 0.0003 0 0.0001 0.0004 0.0004 0.0001 0 0.0001 0
Cytoplasm 0.3291 0.5786 0.5034 0.4264 0.4887 0.56 0.3022 0.5541 0.4977 0.457 0.5769 0.569 0.3615 0.6009 0.5658 0.6965 0.6471 0.6823
Cytoplasmic Foci 0.0181 0.0187 0.0136 0.0164 0.0117 0.0201 0.042 0.0132 0.018 0.0244 0.0165 0.0252 0.0223 0.0116 0.0094 0.011 0.0103 0.0154
Eisosomes 0.0001 0.0001 0.0001 0 0 0 0.0004 0.0001 0.0001 0.0001 0 0.0001 0.0003 0.0001 0 0 0 0
Endoplasmic Reticulum 0.0041 0.0023 0.0036 0.0024 0.0039 0.0024 0.0051 0.0026 0.0038 0.0067 0.0007 0.0034 0.0045 0.0017 0.0024 0.0015 0.0027 0.0014
Endosome 0.0117 0.0069 0.0129 0.0077 0.0097 0.0098 0.0305 0.0052 0.0141 0.0232 0.0073 0.0161 0.0126 0.0021 0.0047 0.0076 0.0059 0.0077
Golgi 0.0007 0.0004 0.0004 0.0002 0.0003 0.0006 0.0077 0.0005 0.0044 0.0085 0.0023 0.0051 0.0021 0.0003 0.0002 0.0003 0.0002 0.0004
Lipid Particles 0.0008 0.0004 0.0005 0.0002 0.0001 0.0003 0.0089 0.0003 0.0039 0.0137 0.0034 0.0021 0.0029 0.0004 0.0001 0 0.0001 0.0002
Mitochondria 0.0017 0.0005 0.0007 0.0005 0.0004 0.0004 0.0049 0.0004 0.0106 0.0034 0.0015 0.0029 0.002 0.0011 0.0004 0.0003 0.0002 0.0005
None 0.609 0.3724 0.453 0.5357 0.4772 0.3961 0.5145 0.3917 0.4028 0.4075 0.3538 0.3452 0.5461 0.3651 0.4084 0.2781 0.3291 0.2817
Nuclear Periphery 0.0026 0.0008 0.0016 0.0009 0.0016 0.0007 0.0041 0.0009 0.0022 0.0019 0.0007 0.0016 0.0024 0.0004 0.0007 0.0004 0.0006 0.0015
Nucleolus 0.0006 0.0004 0.0002 0.0002 0.0001 0.0001 0.0024 0.0002 0.0003 0.001 0.0001 0.0001 0.001 0.0005 0.0002 0 0 0.0002
Nucleus 0.0053 0.0025 0.003 0.0016 0.0021 0.0017 0.0034 0.0011 0.0023 0.0013 0.0009 0.0012 0.0047 0.0013 0.0012 0.0008 0.0009 0.002
Peroxisomes 0.0004 0.0002 0.0002 0.0001 0.0001 0.002 0.0131 0.0002 0.0066 0.0129 0.0267 0.0036 0.0037 0.0003 0.0001 0.0001 0.0001 0.0002
Punctate Nuclear 0.0018 0.0022 0.0009 0.0008 0.0007 0.0012 0.0021 0.0008 0.0019 0.001 0.0038 0.0011 0.0068 0.0007 0.0005 0.0003 0.0003 0.0015
Vacuole 0.0036 0.0022 0.0037 0.0045 0.0021 0.0014 0.006 0.0019 0.0024 0.004 0.0008 0.0019 0.0024 0.0012 0.0019 0.0019 0.0016 0.0008
Vacuole Periphery 0.0006 0.0002 0.0006 0.0006 0.0004 0.0002 0.0011 0.0003 0.0007 0.0014 0.0002 0.0009 0.0004 0.0002 0.0003 0.0004 0.0002 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression – – – – – – – – – –
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression –
Translational Efficiency –
Standard name
Human Ortholog
Description Ub-ligase substrate-specificity factor; proteolytically-cleaved form acts as a pseudosubstrate, binding and altering the substrate specificity of Ubr1p towards misfolded and native ER membrane and cytosolic proteins, as part of the stress-induced homeostatically regulated protein degradation (SHRED) pathway; hydrophilin essential during desiccation-rehydration; induced by osmotic stress, starvation and during stationary phase; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (27%), nucleus (5%), mixed (41%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Roq1

Roq1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Roq1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available