Standard name | |
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Human Ortholog | |
Description | Ub-ligase substrate-specificity factor; proteolytically-cleaved form acts as a pseudosubstrate, binding and altering the substrate specificity of Ubr1p towards misfolded and native ER membrane and cytosolic proteins, as part of the stress-induced homeostatically regulated protein degradation (SHRED) pathway; hydrophilin essential during desiccation-rehydration; induced by osmotic stress, starvation and during stationary phase; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 2 | 0 | – | 1 | 0 | 6 | 3 | 2 | 0 | 0 | 0 | 3 | 1 | 1 | 2 | 1 | 1 | 0 | 0 | 0 |
Bud | 3 | 0 | 4 | 5 | 2 | 1 | – | 6 | 5 | 8 | 13 | 11 | 0 | 0 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 0 | 1 | 4 |
Bud Neck | 1 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 1 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 10 | 4 | 1 | 1 | 6 | 8 | – | 19 | 11 | 26 | 25 | 22 | 0 | 1 | 0 | 5 | 1 | 3 | 0 | 0 | 1 | 0 | 0 | 0 |
Cytoplasm | 610 | 382 | 201 | 311 | 489 | 387 | – | 398 | 236 | 351 | 256 | 371 | 84 | 175 | 159 | 116 | 122 | 129 | 598 | 373 | 176 | 73 | 131 | 128 |
Endoplasmic Reticulum | 3 | 1 | 2 | 0 | 12 | 4 | – | 4 | 6 | 12 | 3 | 17 | 0 | 0 | 0 | 3 | 8 | 6 | 0 | 1 | 2 | 0 | 0 | 0 |
Endosome | 0 | 2 | 0 | 0 | 0 | 1 | – | 3 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 4 | 12 | 4 | 1 | 2 | 0 | 0 | 0 | 2 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 1 | 3 | 4 | 0 | 0 | 0 | 0 |
Mitochondria | 8 | 2 | 12 | 85 | 56 | 121 | – | 198 | 163 | 267 | 245 | 402 | 3 | 2 | 1 | 5 | 9 | 1 | 6 | 8 | 8 | 3 | 3 | 0 |
Nucleus | 0 | 1 | 2 | 0 | 2 | 1 | – | 9 | 1 | 1 | 2 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 0 | – | 12 | 3 | 3 | 4 | 10 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 11 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 6 | 1 | 1 | 0 | 0 | 0 | 0 | 2 |
Vac/Vac Membrane | 0 | 2 | 0 | 0 | 8 | 9 | – | 96 | 32 | 52 | 29 | 27 | 0 | 0 | 1 | 10 | 25 | 14 | 3 | 4 | 0 | 0 | 1 | 1 |
Unique Cell Count | 619 | 389 | 207 | 322 | 530 | 452 | 638 | 363 | 570 | 442 | 683 | 86 | 178 | 160 | 135 | 159 | 148 | 629 | 404 | 199 | 77 | 141 | 144 | |
Labelled Cell Count | 638 | 394 | 223 | 402 | 577 | 532 | 748 | 458 | 730 | 581 | 865 | 88 | 179 | 162 | 154 | 198 | 163 | 629 | 404 | 199 | 77 | 141 | 144 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.3 | 5.0 | 3.5 | 4.1 | 7.1 | 6.9 | – | 6.6 | 6.0 | 6.2 | 5.6 | 5.4 | 5.0 | 5.7 | 5.7 | 8.6 | 9.3 | 8.4 | 4.6 | 5.2 | 5.7 |
Std Deviation (1e-4) | 0.6 | 1.0 | 1.0 | 1.1 | 1.5 | 1.5 | – | 1.7 | 1.8 | 2.1 | 2.4 | 1.9 | 1.0 | 1.1 | 1.1 | 4.3 | 4.2 | 3.8 | 0.8 | 1.3 | 1.5 |
Intensity Change (Log2) | – | – | – | 0.24 | 1.03 | 1.0 | – | 0.94 | 0.78 | 0.84 | 0.68 | 0.63 | 0.52 | 0.73 | 0.72 | 1.32 | 1.42 | 1.28 | 0.39 | 0.57 | 0.72 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | 0.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 2.7 | 3.1 | 2.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.3 | -2.4 | -4.4 | – | -9.6 | -8.7 | -9.7 | -10.2 | -11.3 | 0.3 | 0.8 | 1.6 | -3.9 | -6.0 | -3.6 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 2.0 | 6.2 | – | 7.3 | 9.7 | 10.5 | 12.0 | 13.4 | 0 | -2.4 | -2.7 | -0.9 | -0.1 | -2.5 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | – | 5.9 | 4.4 | 4.5 | 3.8 | 2.9 | 0 | 0 | 0 | 0 | 5.9 | 4.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.5969 | 1.5754 | 0.961 | 0.9132 | 0.7666 | 1.0945 | -0.3929 | 0.3872 | 0.1532 | 0.6008 | -0.282 | 0.3192 | 0.9829 | 2.396 | 1.888 | 1.8201 | 1.954 | 1.9276 |
Actin | 0.0006 | 0.0006 | 0.0003 | 0.0001 | 0.0003 | 0.0013 | 0.0176 | 0.0045 | 0.011 | 0.0027 | 0.0021 | 0.0107 | 0.0119 | 0.0004 | 0.0004 | 0.0001 | 0.0001 | 0.0013 |
Bud | 0.0036 | 0.001 | 0.0004 | 0.0005 | 0.0002 | 0.0002 | 0.0029 | 0.0027 | 0.0032 | 0.0007 | 0.0006 | 0.001 | 0.001 | 0.0071 | 0.0014 | 0.0002 | 0.0001 | 0.0001 |
Bud Neck | 0.0003 | 0.0004 | 0.0002 | 0.0001 | 0.0002 | 0.0012 | 0.0143 | 0.0002 | 0.0008 | 0.0205 | 0.0001 | 0.0061 | 0.003 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0026 |
Bud Periphery | 0.0021 | 0.0006 | 0.0004 | 0.0008 | 0.0002 | 0.0001 | 0.007 | 0.0067 | 0.0037 | 0.0007 | 0.0008 | 0.0024 | 0.0014 | 0.0016 | 0.0004 | 0.0001 | 0.0001 | 0.0001 |
Bud Site | 0.0027 | 0.0084 | 0.0006 | 0.0003 | 0.0001 | 0.0002 | 0.0091 | 0.012 | 0.0093 | 0.0073 | 0.0007 | 0.0004 | 0.0067 | 0.0026 | 0.0013 | 0.0001 | 0.0001 | 0.0001 |
Cell Periphery | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0.0007 | 0.0004 | 0.0003 | 0.0003 | 0 | 0.0001 | 0.0004 | 0.0004 | 0.0001 | 0 | 0.0001 | 0 |
Cytoplasm | 0.3291 | 0.5786 | 0.5034 | 0.4264 | 0.4887 | 0.56 | 0.3022 | 0.5541 | 0.4977 | 0.457 | 0.5769 | 0.569 | 0.3615 | 0.6009 | 0.5658 | 0.6965 | 0.6471 | 0.6823 |
Cytoplasmic Foci | 0.0181 | 0.0187 | 0.0136 | 0.0164 | 0.0117 | 0.0201 | 0.042 | 0.0132 | 0.018 | 0.0244 | 0.0165 | 0.0252 | 0.0223 | 0.0116 | 0.0094 | 0.011 | 0.0103 | 0.0154 |
Eisosomes | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0003 | 0.0001 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0041 | 0.0023 | 0.0036 | 0.0024 | 0.0039 | 0.0024 | 0.0051 | 0.0026 | 0.0038 | 0.0067 | 0.0007 | 0.0034 | 0.0045 | 0.0017 | 0.0024 | 0.0015 | 0.0027 | 0.0014 |
Endosome | 0.0117 | 0.0069 | 0.0129 | 0.0077 | 0.0097 | 0.0098 | 0.0305 | 0.0052 | 0.0141 | 0.0232 | 0.0073 | 0.0161 | 0.0126 | 0.0021 | 0.0047 | 0.0076 | 0.0059 | 0.0077 |
Golgi | 0.0007 | 0.0004 | 0.0004 | 0.0002 | 0.0003 | 0.0006 | 0.0077 | 0.0005 | 0.0044 | 0.0085 | 0.0023 | 0.0051 | 0.0021 | 0.0003 | 0.0002 | 0.0003 | 0.0002 | 0.0004 |
Lipid Particles | 0.0008 | 0.0004 | 0.0005 | 0.0002 | 0.0001 | 0.0003 | 0.0089 | 0.0003 | 0.0039 | 0.0137 | 0.0034 | 0.0021 | 0.0029 | 0.0004 | 0.0001 | 0 | 0.0001 | 0.0002 |
Mitochondria | 0.0017 | 0.0005 | 0.0007 | 0.0005 | 0.0004 | 0.0004 | 0.0049 | 0.0004 | 0.0106 | 0.0034 | 0.0015 | 0.0029 | 0.002 | 0.0011 | 0.0004 | 0.0003 | 0.0002 | 0.0005 |
None | 0.609 | 0.3724 | 0.453 | 0.5357 | 0.4772 | 0.3961 | 0.5145 | 0.3917 | 0.4028 | 0.4075 | 0.3538 | 0.3452 | 0.5461 | 0.3651 | 0.4084 | 0.2781 | 0.3291 | 0.2817 |
Nuclear Periphery | 0.0026 | 0.0008 | 0.0016 | 0.0009 | 0.0016 | 0.0007 | 0.0041 | 0.0009 | 0.0022 | 0.0019 | 0.0007 | 0.0016 | 0.0024 | 0.0004 | 0.0007 | 0.0004 | 0.0006 | 0.0015 |
Nucleolus | 0.0006 | 0.0004 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0024 | 0.0002 | 0.0003 | 0.001 | 0.0001 | 0.0001 | 0.001 | 0.0005 | 0.0002 | 0 | 0 | 0.0002 |
Nucleus | 0.0053 | 0.0025 | 0.003 | 0.0016 | 0.0021 | 0.0017 | 0.0034 | 0.0011 | 0.0023 | 0.0013 | 0.0009 | 0.0012 | 0.0047 | 0.0013 | 0.0012 | 0.0008 | 0.0009 | 0.002 |
Peroxisomes | 0.0004 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.002 | 0.0131 | 0.0002 | 0.0066 | 0.0129 | 0.0267 | 0.0036 | 0.0037 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0002 |
Punctate Nuclear | 0.0018 | 0.0022 | 0.0009 | 0.0008 | 0.0007 | 0.0012 | 0.0021 | 0.0008 | 0.0019 | 0.001 | 0.0038 | 0.0011 | 0.0068 | 0.0007 | 0.0005 | 0.0003 | 0.0003 | 0.0015 |
Vacuole | 0.0036 | 0.0022 | 0.0037 | 0.0045 | 0.0021 | 0.0014 | 0.006 | 0.0019 | 0.0024 | 0.004 | 0.0008 | 0.0019 | 0.0024 | 0.0012 | 0.0019 | 0.0019 | 0.0016 | 0.0008 |
Vacuole Periphery | 0.0006 | 0.0002 | 0.0006 | 0.0006 | 0.0004 | 0.0002 | 0.0011 | 0.0003 | 0.0007 | 0.0014 | 0.0002 | 0.0009 | 0.0004 | 0.0002 | 0.0003 | 0.0004 | 0.0002 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | – | – | – | – | – | – | – | – | – | – |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | – |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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