Standard name
Human Ortholog
Description Non-classical phosphatidylinositol transfer protein (PITP); exhibits PI- but not PC-transfer activity; localizes to the peripheral endoplasmic reticulum, cytosol and microsomes; similar to Sec14p; partially relocalizes to the plasma membrane upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0.06 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.05 0.09 0.06 0.24 0.45 0.25 0.16 0.11 0.14 0.17 0.17 0.16 0.21 0.48 0.63 0.09 0.09 0.11 0 0 0 0 0 0
Cytoplasm 0.98 0.97 0.98 0.94 0.73 0.9 0.89 0.92 0.82 0.87 0.76 0.78 0.84 0.87 0.8 0.93 0.95 0.96 0.96 0.95 0.92 0.81 0.91 0.91
Endoplasmic Reticulum 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0.09 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.05 0.06 0 0.2 0.14 0.26 0.18 0 0.06 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 20 6 3 3 3 5 10 15 3 2 2 0 0 0 0 0 1 0 0 2
Bud 0 3 4 0 6 2 4 9 8 9 20 13 1 5 0 1 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 1 0 1 2 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 8 25 17 60 157 98 94 72 78 109 98 92 8 41 104 8 9 14 0 0 1 0 0 0
Cytoplasm 171 282 286 239 251 356 523 601 453 560 427 459 32 74 131 86 96 120 166 281 278 20 92 114
Endoplasmic Reticulum 3 2 1 5 18 4 14 4 7 11 8 18 1 1 1 8 4 5 0 0 0 0 0 1
Endosome 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 1 1 2 1 2 0 0 0
Golgi 1 2 2 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 3 4 0 0 1
Mitochondria 0 3 0 0 1 18 37 22 111 92 149 105 1 5 2 0 1 0 1 1 5 0 0 1
Nucleus 2 0 2 2 2 1 3 1 2 3 2 2 1 0 4 1 2 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 2 0 3 1 2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 1 0 0 0 0 0 1 0 2 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 1 0 1 1 1 1 0 0 0 4 1 2 0 0 0 0 0 0
Vac/Vac Membrane 0 2 4 2 2 3 10 18 6 8 6 20 0 0 0 2 1 2 0 2 1 0 2 0
Unique Cell Count 174 290 293 253 346 396 586 654 550 643 563 589 38 85 164 92 101 125 174 295 303 25 102 125
Labelled Cell Count 185 319 317 309 460 489 691 734 670 802 725 728 47 129 244 111 115 144 174 295 303 25 102 125


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.8 8.1 8.6 8.3 8.2 6.5 6.4 6.7 5.6 5.8 5.6 5.8 7.6 8.8 9.0 7.7 8.9 9.8 – 8.2 9.4
Std Deviation (1e-4) 1.2 1.3 1.4 1.3 1.3 1.2 1.2 1.4 1.2 1.0 1.4 1.0 1.2 1.5 1.5 1.8 1.9 1.9 – 1.7 1.5
Intensity Change (Log2) – – – -0.06 -0.07 -0.4 -0.42 -0.37 -0.62 -0.57 -0.62 -0.58 -0.18 0.03 0.06 -0.16 0.05 0.18 – -0.07 0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 4.2 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 1.8 0.9 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 6.0 11.2 6.6 4.3 2.5 3.7 4.6 4.7 4.2 0 9.6 13.4 1.0 1.1 1.9
Cytoplasm -1.9 -8.6 -4.0 -4.3 -3.3 -6.4 -5.1 -8.1 -7.6 -4.0 -4.0 -6.4 -1.9 -1.3 -0.9
Endoplasmic Reticulum 0 3.6 0 2.2 0 0 0 0 2.6 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.7 4.4 3.2 8.3 6.8 9.7 7.7 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 1.3 0 0 0 1.7 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.9958 7.5618 6.8142 6.3352 5.7503 6.5024 5.1713 6.8654 5.5763 4.8639 5.1381 5.7999 3.4189 5.0699 4.391 4.1672 3.2403 4.4678
Actin 0.0515 0.0013 0.0054 0.014 0.056 0.0086 0.0594 0.0026 0.0136 0.0454 0.0246 0.0052 0.0119 0.0059 0.0038 0.0014 0.0838 0.0079
Bud 0.0023 0.0001 0.0018 0.0001 0.0004 0.0005 0.0008 0.0001 0.0045 0.0003 0.0002 0.0004 0.0021 0.002 0.0001 0.0001 0.0015 0.0003
Bud Neck 0.0032 0.0007 0.0012 0.001 0.0025 0.006 0.0031 0.0006 0.001 0.0021 0.0025 0.0057 0.001 0.0005 0.0007 0.0022 0.0024 0.0046
Bud Periphery 0.002 0.0001 0.0033 0.0001 0.0004 0.0004 0.0017 0.0001 0.0037 0.001 0.0003 0.0012 0.0017 0.0007 0.0001 0.0001 0.0032 0.0007
Bud Site 0.016 0.0054 0.0045 0.0009 0.0016 0.0004 0.0061 0.0045 0.0142 0.0009 0.0009 0.0007 0.0029 0.0036 0.0018 0.0002 0.0082 0.0004
Cell Periphery 0.0035 0.0051 0.0128 0.0007 0.0021 0.0059 0.0034 0.0029 0.0021 0.0005 0.0016 0.0011 0.0011 0.0021 0.0012 0.0005 0.0007 0.0126
Cytoplasm 0.7423 0.9729 0.9202 0.9229 0.8568 0.9335 0.712 0.979 0.806 0.8676 0.7255 0.9538 0.8652 0.9645 0.9766 0.9885 0.7359 0.9632
Cytoplasmic Foci 0.0403 0.0008 0.0029 0.0259 0.0231 0.0069 0.0272 0.0009 0.0181 0.0094 0.0351 0.0022 0.0224 0.0011 0.0014 0.0008 0.0559 0.0011
Eisosomes 0.0005 0.0001 0.0001 0 0.0005 0.0001 0.0004 0 0.0002 0.0003 0.0001 0 0.0001 0 0 0 0.0005 0
Endoplasmic Reticulum 0.0069 0.002 0.0034 0.0012 0.0056 0.0062 0.009 0.0018 0.0034 0.0023 0.0036 0.0049 0.0107 0.0021 0.0024 0.0028 0.006 0.0021
Endosome 0.0199 0.0001 0.0054 0.0024 0.0229 0.0017 0.0258 0.0001 0.0141 0.003 0.0197 0.0053 0.01 0.0002 0.0003 0.0003 0.0241 0.0016
Golgi 0.0112 0.0001 0.0013 0.0043 0.018 0.0032 0.0136 0.0002 0.0091 0.0055 0.024 0.0028 0.0031 0.0001 0.0002 0.0001 0.017 0.0009
Lipid Particles 0.0127 0.0001 0.003 0.0022 0.0029 0.0028 0.0089 0.0001 0.0098 0.0048 0.0124 0.0004 0.0058 0.0003 0.001 0 0.0172 0.0002
Mitochondria 0.0115 0 0.0015 0.0007 0.0029 0.0014 0.0058 0 0.0083 0.0398 0.0299 0.0011 0.0008 0.0001 0.0003 0 0.0095 0.0001
None 0.0304 0.0104 0.016 0.0023 0.0006 0.0123 0.0975 0.0062 0.0731 0.0025 0.0302 0.0121 0.0436 0.0153 0.002 0.0018 0.0058 0.0028
Nuclear Periphery 0.0103 0.0001 0.003 0.0001 0.0002 0.0005 0.0012 0.0002 0.0016 0.0002 0.0449 0.0009 0.002 0.0002 0.0002 0.0003 0.002 0.0004
Nucleolus 0.001 0 0.0007 0 0 0.0001 0.0002 0 0.0006 0 0.0002 0.0001 0.0007 0 0 0 0.0058 0
Nucleus 0.0089 0.0004 0.0021 0.0002 0.0003 0.0009 0.0007 0.0004 0.0008 0.0004 0.0039 0.0008 0.0032 0.0006 0.0004 0.0006 0.0032 0.0005
Peroxisomes 0.0164 0 0.0064 0.0206 0.0019 0.0071 0.0152 0 0.0122 0.0127 0.0362 0.0001 0.0049 0.0001 0.0073 0 0.0054 0.0001
Punctate Nuclear 0.0068 0.0001 0.0011 0.0002 0.0002 0.0008 0.0057 0.0001 0.0018 0.0003 0.0027 0.0002 0.0051 0.0002 0.0001 0.0001 0.003 0.0004
Vacuole 0.0014 0.0002 0.0035 0.0001 0.0009 0.0005 0.0019 0.0001 0.0014 0.0005 0.0005 0.0008 0.0016 0.0004 0.0002 0.0002 0.0074 0.0002
Vacuole Periphery 0.001 0 0.0005 0 0.0002 0.0001 0.0006 0 0.0004 0.0006 0.001 0.0002 0.0003 0 0 0 0.0016 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 46.131 28.9783 45.4874 55.6703 43.6458 61.0586 47.0489 47.9175 49.231 61.4465
Translational Efficiency 2.3099 3.8416 2.4479 1.8026 2.3694 1.598 2.0136 2.1996 1.9935 1.6973

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Non-classical phosphatidylinositol transfer protein (PITP); exhibits PI- but not PC-transfer activity; localizes to the peripheral endoplasmic reticulum, cytosol and microsomes; similar to Sec14p; partially relocalizes to the plasma membrane upon DNA replication stress
Localization
Cell Percentages cytoplasm (89%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-10

Sfh5

Sfh5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sfh5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available