Standard name
Human Ortholog
Description Type I membrane protein involved in autophagy and the Cvt pathway; may be involved in membrane delivery to the phagophore assembly site
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0.11 0.09 0 0.05 0.07 0.05 0.05 0 0.05 0.09 0.06 0.05 0.12 0.08 0.05 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0.07 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.34 0.39 0.3 0.37 0.48 0.42 0.57 0.57 0.62 0.61 0.62 0.52 0.2 0.1 0.16 0.29 0.18 0.13 0 0 0 0 0 0
Endoplasmic Reticulum 0.07 0.11 0.18 0.19 0.08 0.13 0.13 0 0 0.07 0 0.07 0.28 0.17 0.09 0.05 0 0 0.05 0 0.05 0.06 0 0.05
Endosome 0.13 0 0.14 0.17 0.22 0.13 0.17 0.23 0.12 0.15 0.1 0.17 0.1 0.16 0.19 0.18 0.19 0.05 0.3 0.22 0.32 0.34 0.39 0.35
Golgi 0.05 0 0.12 0.2 0.06 0 0 0 0 0 0 0 0.38 0.46 0.45 0.25 0.19 0.45 0.09 0.08 0.13 0.33 0.3 0.41
Mitochondria 0 0 0 0 0 0.25 0 0 0.12 0.08 0.17 0.12 0 0 0 0.06 0.07 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.59 0.55 0.42 0.19 0.25 0.23 0.18 0.19 0.13 0.12 0.13 0.16 0.22 0.28 0.28 0.4 0.49 0.37 0.49 0.58 0.42 0.2 0.2 0.13
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 8 7 2 18 25 2 11 27 5 10 4 9 35 32 28 14 7 2 1 0 1 3 6 7
Bud 0 1 0 0 4 2 1 16 7 10 4 4 2 1 4 1 0 0 1 2 0 1 1 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 1 3 0 3 1 2 0 1 0 0 0 0 0 0 0 0 0 0
Cytoplasm 155 89 39 60 135 29 140 209 66 124 63 91 74 53 91 34 16 5 17 9 2 3 8 10
Endoplasmic Reticulum 31 25 24 30 22 9 31 7 2 15 1 12 105 90 50 6 2 1 25 11 7 30 19 29
Endosome 62 3 18 28 62 9 42 84 13 30 10 30 36 86 106 21 17 2 153 61 49 162 193 196
Golgi 21 9 15 33 18 0 5 3 0 1 0 0 144 245 254 29 17 17 45 22 19 157 148 233
Mitochondria 3 3 0 1 0 17 1 8 13 17 17 21 5 17 15 7 6 0 7 3 4 9 6 4
Nucleus 0 2 0 1 0 1 0 2 1 2 1 2 2 0 1 0 0 0 1 0 0 0 1 0
Nuclear Periphery 0 0 0 0 0 0 0 1 1 0 1 0 0 0 0 0 0 0 6 1 1 3 2 2
Nucleolus 2 1 1 0 0 1 0 1 0 0 2 0 0 3 2 1 1 0 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0
SpindlePole 0 0 0 0 3 0 2 3 1 3 0 2 2 1 3 1 1 0 1 0 0 1 1 0
Vac/Vac Membrane 273 125 55 30 70 16 45 71 14 25 13 28 82 151 159 47 44 14 249 163 63 92 96 75
Unique Cell Count 460 229 130 161 281 69 244 368 107 204 102 174 378 538 570 117 89 38 514 281 152 470 494 566
Labelled Cell Count 555 265 154 201 339 86 279 435 123 240 117 202 487 680 713 161 111 41 514 281 152 470 494 566


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.1 12.7 12.6 12.1 11.9 6.4 8.8 8.4 7.7 7.5 7.2 7.4 12.5 13.0 12.9 14.6 14.4 16.1 11.5 11.9 12.1
Std Deviation (1e-4) 2.1 2.4 2.2 1.7 1.9 1.7 1.6 1.5 1.5 1.5 1.5 1.5 2.1 2.3 2.3 3.5 3.0 3.3 2.3 2.4 2.3
Intensity Change (Log2) – – – -0.06 -0.09 -0.97 -0.52 -0.59 -0.7 -0.74 -0.81 -0.77 -0.01 0.04 0.03 0.21 0.19 0.35 -0.13 -0.08 -0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 3.2 2.8 0 0 2.4 0 0 0 0 2.9 2.1 1.7 3.3 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.3 3.4 1.7 5.0 5.3 4.9 5.5 4.8 3.9 -2.5 -6.0 -3.7 -0.2 -2.0 -2.1
Endoplasmic Reticulum 0 -3.2 -1.0 -1.5 -6.7 -4.1 -3.1 -4.3 -3.1 2.1 -0.5 -3.2 -3.2 -3.6 -2.4
Endosome 0.8 2.0 -0.2 0.8 2.2 -0.4 0.2 -0.9 0.8 -1.4 0.6 1.3 0.9 1.0 0
Golgi 2.0 -1.8 -2.9 -3.9 0 -3.6 -4.6 -3.5 -4.6 5.6 7.1 7.0 2.7 1.6 4.6
Mitochondria 0 0 5.9 0 0 4.1 3.4 4.8 4.1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -4.4 -3.6 -2.7 -5.0 -5.2 -4.9 -6.3 -4.9 -5.1 -4.6 -3.2 -3.2 -0.3 1.0 -0.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.0764 6.5629 6.1725 5.3631 4.4039 5.6988 6.8614 8.4494 8.1109 8.1714 7.6611 8.4652 8.9119 10.398 9.6257 9.9273 9.2026 10.0704
Actin 0.0159 0.0002 0.0078 0 0.0155 0.0001 0.0227 0 0.0102 0 0.0016 0.0007 0.0398 0.0011 0.0082 0.0019 0 0.0001
Bud 0.0002 0.0006 0.0013 0.0001 0.0003 0.0001 0.0005 0.0002 0.0005 0.0003 0.0002 0.0002 0.0005 0.0003 0.0013 0.0001 0 0.0004
Bud Neck 0.0003 0.0003 0.0006 0.0001 0.001 0.0001 0.001 0.0002 0.0003 0.0002 0.0004 0.0004 0.0071 0.0002 0.0004 0.0001 0.0001 0.0004
Bud Periphery 0.0002 0.0003 0.0008 0.0001 0.0004 0.0001 0.0006 0.0002 0.0006 0.0002 0.0002 0.0002 0.0006 0.0002 0.001 0.0001 0 0.0004
Bud Site 0.0004 0.0005 0.0017 0 0.0015 0.0001 0.0013 0.001 0.0009 0.0002 0.0004 0.0001 0.0026 0.0004 0.001 0.0002 0 0.0003
Cell Periphery 0.0001 0.0001 0.0002 0 0.0001 0 0.0003 0.0002 0.0002 0.0001 0.0001 0.0001 0.0006 0.0002 0.0003 0.0001 0 0.0001
Cytoplasm 0.019 0.0465 0.0421 0.0096 0.008 0.0202 0.0311 0.0646 0.0321 0.0662 0.0165 0.0419 0.0605 0.0962 0.0356 0.0071 0.0038 0.0458
Cytoplasmic Foci 0.0251 0.0308 0.0292 0.0094 0.0234 0.0192 0.0226 0.0214 0.0255 0.0075 0.0199 0.0126 0.0229 0.02 0.0165 0.0243 0.0141 0.0123
Eisosomes 0.0001 0 0.0001 0 0.0001 0 0.0002 0 0.0001 0 0.0001 0 0.0005 0 0 0 0 0
Endoplasmic Reticulum 0.0162 0.0099 0.012 0.0043 0.0136 0.0074 0.0199 0.0108 0.0105 0.0038 0.003 0.0126 0.035 0.0164 0.0129 0.0017 0.0025 0.0127
Endosome 0.6553 0.6092 0.6068 0.6107 0.7284 0.6861 0.5195 0.4278 0.4965 0.3602 0.5259 0.4948 0.4255 0.4709 0.4354 0.4713 0.5013 0.4541
Golgi 0.0567 0.0543 0.0606 0.0103 0.0609 0.0586 0.0338 0.012 0.0253 0.0063 0.014 0.0201 0.0519 0.0402 0.0472 0.0289 0.0419 0.0364
Lipid Particles 0.0149 0.0018 0.003 0.0008 0.0082 0.0013 0.0117 0.0015 0.0094 0.0004 0.0184 0.0061 0.0193 0.0026 0.0063 0.0107 0.0004 0.0011
Mitochondria 0.0134 0.0066 0.0096 0.0018 0.0023 0.0065 0.0163 0.0009 0.0134 0.0006 0.003 0.0027 0.0079 0.0073 0.0107 0.0039 0.0005 0.0097
None 0.0013 0.0004 0.0097 0.0001 0.0005 0.0096 0.0028 0.0002 0.0008 0.0002 0.0004 0.0004 0.0027 0.0068 0.0005 0.0002 0 0.0004
Nuclear Periphery 0.0174 0.0085 0.0182 0.0052 0.0057 0.0059 0.0382 0.0129 0.0185 0.0084 0.0035 0.0256 0.0756 0.023 0.0254 0.0042 0.008 0.0181
Nucleolus 0.0002 0.0001 0.0002 0 0.0002 0 0.001 0.0002 0.0007 0.0002 0.0009 0.0001 0.0006 0.0001 0.0006 0.0001 0 0.0002
Nucleus 0.001 0.0009 0.0015 0.0004 0.0005 0.0005 0.0107 0.0024 0.0022 0.0019 0.0009 0.0022 0.0231 0.0026 0.0031 0.0006 0.0005 0.0066
Peroxisomes 0.0034 0.0004 0.0034 0 0.0043 0.0001 0.003 0.0001 0.0022 0 0.0101 0.0002 0.0013 0.0002 0.0006 0.0117 0 0.0001
Punctate Nuclear 0.0009 0.0001 0.0007 0 0.002 0.0001 0.0042 0.0001 0.0008 0.0001 0.0041 0.0006 0.0038 0.0003 0.0002 0.0006 0 0.0002
Vacuole 0.092 0.1447 0.1181 0.163 0.0674 0.0888 0.17 0.329 0.2353 0.3637 0.2606 0.2099 0.1419 0.2082 0.2465 0.2864 0.2513 0.192
Vacuole Periphery 0.0658 0.0839 0.0724 0.1837 0.0557 0.0951 0.0887 0.1141 0.114 0.1797 0.1162 0.1685 0.0764 0.1027 0.1463 0.1458 0.1753 0.2086

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 60.8495 62.8573 62.7884 65.7837 64.8684 63.5187 59.8883 55.6814 71.9096 64.0543
Translational Efficiency 1.2778 1.3181 1.288 1.4284 1.3054 1.2321 1.2483 1.4881 1.0406 1.1655

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Type I membrane protein involved in autophagy and the Cvt pathway; may be involved in membrane delivery to the phagophore assembly site
Localization
Cell Percentages vacuolar membrane (31%)
Cell Cycle Regulation No
Subcompartmental Group vac memb-1

Atg27

Atg27


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Atg27-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available