Standard name | |
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Human Ortholog | |
Description | Subunit of a Golgi mannosyltransferase complex; this complex also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | – | – | 1 | 6 | 15 | 4 | – | 10 | 8 | 2 | 4 | 7 | 14 | 27 | 15 | 0 | 1 | 2 | 0 | 1 | 5 | 0 | 2 | 2 |
Bud | – | – | 5 | 12 | 20 | 18 | – | 35 | 46 | 39 | 33 | 38 | 0 | 4 | 3 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Neck | – | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | – | – | 0 | 0 | 0 | 0 | – | 5 | 4 | 4 | 5 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | – | – | 0 | 0 | 0 | 0 | – | 0 | 2 | 1 | 1 | 0 | 0 | 2 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | – | – | 32 | 21 | 31 | 13 | – | 15 | 8 | 4 | 6 | 6 | 5 | 46 | 94 | 43 | 55 | 58 | 1 | 2 | 0 | 0 | 3 | 2 |
Endoplasmic Reticulum | – | – | 12 | 3 | 10 | 4 | – | 2 | 3 | 2 | 1 | 2 | 19 | 26 | 17 | 3 | 5 | 5 | 0 | 0 | 1 | 2 | 3 | 7 |
Endosome | – | – | 128 | 125 | 182 | 145 | – | 135 | 24 | 38 | 16 | 42 | 12 | 40 | 19 | 3 | 39 | 36 | 15 | 13 | 159 | 32 | 75 | 60 |
Golgi | – | – | 43 | 44 | 71 | 43 | – | 22 | 9 | 8 | 4 | 6 | 5 | 9 | 2 | 0 | 8 | 9 | 18 | 1 | 150 | 21 | 56 | 37 |
Mitochondria | – | – | 96 | 70 | 81 | 275 | – | 364 | 347 | 545 | 323 | 433 | 8 | 13 | 4 | 3 | 16 | 14 | 9 | 10 | 20 | 12 | 26 | 23 |
Nucleus | – | – | 0 | 0 | 0 | 0 | – | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 4 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | – | – | 3 | 0 | 1 | 1 | – | 3 | 2 | 5 | 1 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Nucleolus | – | – | 1 | 7 | 5 | 26 | – | 56 | 26 | 41 | 13 | 20 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | – | – | 5 | 4 | 28 | 37 | – | 135 | 55 | 66 | 34 | 75 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 13 | 1 | 7 | 1 |
SpindlePole | – | – | 13 | 20 | 32 | 29 | – | 87 | 35 | 40 | 31 | 35 | 8 | 27 | 6 | 0 | 4 | 3 | 0 | 0 | 1 | 0 | 0 | 0 |
Vac/Vac Membrane | – | – | 87 | 22 | 20 | 16 | – | 10 | 5 | 5 | 3 | 7 | 4 | 10 | 12 | 25 | 69 | 66 | 19 | 13 | 39 | 10 | 52 | 122 |
Unique Cell Count | 323 | 241 | 369 | 480 | 631 | 430 | 645 | 381 | 522 | 62 | 178 | 161 | 63 | 158 | 142 | 71 | 46 | 394 | 85 | 233 | 265 | |||
Labelled Cell Count | 426 | 334 | 496 | 611 | 881 | 574 | 800 | 476 | 682 | 75 | 207 | 181 | 81 | 200 | 197 | 71 | 46 | 394 | 85 | 233 | 265 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | – | – | 6.7 | 7.7 | 7.7 | 7.2 | – | 7.3 | 7.1 | 6.6 | 6.7 | 7.1 | 6.8 | 6.5 | 6.2 | 5.6 | 7.0 | 8.2 | 7.4 | 7.6 | 7.5 |
Std Deviation (1e-4) | – | – | 1.2 | 1.7 | 1.6 | 1.2 | – | 1.3 | 1.4 | 1.3 | 1.4 | 1.3 | 0.9 | 1.0 | 0.9 | 1.0 | 1.4 | 1.6 | 1.3 | 1.5 | 1.8 |
Intensity Change (Log2) | – | – | – | 0.2 | 0.21 | 0.1 | – | 0.13 | 0.09 | -0.02 | 0.01 | 0.08 | 0.02 | -0.04 | -0.11 | -0.25 | 0.07 | 0.29 | 0.14 | 0.18 | 0.17 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 3.3 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 6.9 | 5.2 | 0 | 0 | 0 |
Bud | 2.4 | 2.7 | 1.8 | – | 2.9 | 4.9 | 3.2 | 4.2 | 3.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.5 | -0.7 | -4.3 | – | -5.1 | -4.9 | -7.2 | -4.9 | -6.0 | -0.5 | 4.7 | 11.5 | 10.7 | 6.7 | 7.8 |
Endoplasmic Reticulum | -1.8 | -0.8 | -2.9 | – | 0 | -2.9 | 0 | -3.4 | -3.7 | 0 | 4.4 | 3.0 | 0 | -0.3 | -0.1 |
Endosome | 2.9 | 2.6 | -2.8 | – | -6.0 | -11.5 | -13.1 | -11.6 | -11.1 | -3.0 | -3.9 | -6.3 | -5.3 | -3.2 | -3.0 |
Golgi | 1.6 | 2.1 | -2.0 | – | -5.7 | -6.0 | -7.9 | -6.5 | -7.4 | -1.1 | -2.9 | -4.3 | -3.1 | -2.8 | -2.2 |
Mitochondria | -0.2 | -2.3 | 7.7 | – | 8.2 | 14.1 | 17.0 | 14.8 | 15.5 | -2.7 | -5.8 | -7.0 | -4.1 | -4.8 | -4.6 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 3.9 | – | 5.3 | 4.2 | 4.4 | 2.9 | 3.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 3.7 | 3.8 | – | 8.2 | 5.6 | 4.9 | 4.3 | 6.2 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 2.1 | 2.5 | 1.3 | – | 4.7 | 2.3 | 1.4 | 2.2 | 1.6 | 0 | 4.4 | -0.2 | 0 | -0.8 | 0 |
Vacuole | -5.3 | -7.8 | -9.8 | – | -12.3 | -10.7 | -13.1 | -10.4 | -11.5 | -3.5 | -5.8 | -5.0 | 2.0 | 3.7 | 4.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.2135 | 5.5351 | 4.782 | 5.3625 | 4.5849 | 4.9952 | 4.3538 | 6.594 | 5.7137 | 4.9707 | 5.2354 | 5.8213 | 4.9152 | 4.207 | 4.0103 | 5.9059 | 5.5306 | 4.4653 |
Actin | 0.0357 | 0.0083 | 0.0273 | 0.0006 | 0.0022 | 0.0045 | 0.0083 | 0.0027 | 0.0019 | 0.0008 | 0.0061 | 0.0111 | 0.0049 | 0.0028 | 0.0028 | 0.0038 | 0.0001 | 0.001 |
Bud | 0.0006 | 0.0045 | 0.0051 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0005 | 0 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0 | 0 |
Bud Neck | 0.0035 | 0.0004 | 0.0007 | 0.0001 | 0.0004 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0003 | 0.0003 | 0.0002 | 0.0004 | 0.0002 | 0.0002 | 0.0004 | 0.0001 | 0.0001 |
Bud Periphery | 0.0009 | 0.0015 | 0.0023 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0008 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0 | 0 |
Bud Site | 0.0057 | 0.0108 | 0.0033 | 0.0004 | 0.0002 | 0.0001 | 0.0003 | 0.0006 | 0.0001 | 0.0002 | 0.0011 | 0.0001 | 0.0006 | 0.0001 | 0.0003 | 0.0003 | 0 | 0 |
Cell Periphery | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 |
Cytoplasm | 0.0147 | 0.0046 | 0.0017 | 0.0018 | 0.001 | 0.0033 | 0.0018 | 0.0011 | 0.0008 | 0.0008 | 0.0008 | 0.0006 | 0.0014 | 0.0005 | 0.0005 | 0.0004 | 0.0002 | 0.0003 |
Cytoplasmic Foci | 0.089 | 0.0974 | 0.0966 | 0.178 | 0.1219 | 0.086 | 0.0761 | 0.06 | 0.0553 | 0.0596 | 0.0835 | 0.0498 | 0.065 | 0.0884 | 0.0567 | 0.068 | 0.023 | 0.0503 |
Eisosomes | 0.0002 | 0 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0046 | 0.0008 | 0.0006 | 0.0005 | 0.0617 | 0.0019 | 0.0014 | 0.0004 | 0.0004 | 0.0008 | 0.0004 | 0.0004 | 0.0011 | 0.0005 | 0.0004 | 0.0001 | 0.0004 | 0.0004 |
Endosome | 0.2738 | 0.312 | 0.2594 | 0.292 | 0.1274 | 0.2575 | 0.253 | 0.2834 | 0.2188 | 0.31 | 0.2825 | 0.1761 | 0.4453 | 0.1955 | 0.2513 | 0.3086 | 0.3032 | 0.1942 |
Golgi | 0.4558 | 0.496 | 0.5193 | 0.4755 | 0.588 | 0.5644 | 0.4974 | 0.6101 | 0.6784 | 0.5166 | 0.5407 | 0.6767 | 0.375 | 0.5868 | 0.5465 | 0.5359 | 0.6081 | 0.4877 |
Lipid Particles | 0.0362 | 0.0081 | 0.0137 | 0.0203 | 0.0391 | 0.0174 | 0.0284 | 0.004 | 0.0036 | 0.0077 | 0.0096 | 0.0143 | 0.0436 | 0.099 | 0.0063 | 0.0045 | 0.0125 | 0.2144 |
Mitochondria | 0.0254 | 0.0171 | 0.0386 | 0.0129 | 0.0366 | 0.0335 | 0.0386 | 0.0262 | 0.0293 | 0.0152 | 0.0547 | 0.0357 | 0.0346 | 0.0128 | 0.1162 | 0.0291 | 0.0061 | 0.0183 |
None | 0.0025 | 0.0003 | 0.0004 | 0.0016 | 0.0001 | 0.0002 | 0.0117 | 0 | 0 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0 | 0 |
Nuclear Periphery | 0.0045 | 0.0004 | 0.0003 | 0.0004 | 0.0004 | 0.0077 | 0.0146 | 0.0001 | 0.0001 | 0.0005 | 0.0002 | 0.0001 | 0.0006 | 0.0002 | 0.0001 | 0 | 0.0006 | 0.0003 |
Nucleolus | 0.0002 | 0.0004 | 0.0002 | 0.0011 | 0.0001 | 0.0001 | 0.0002 | 0 | 0 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0.0017 | 0.0002 | 0.0001 | 0.0004 | 0 | 0.003 | 0.0005 | 0 | 0 | 0.0002 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0.0357 | 0.0182 | 0.0192 | 0.0079 | 0.0185 | 0.011 | 0.027 | 0.004 | 0.007 | 0.005 | 0.0122 | 0.0276 | 0.0088 | 0.0075 | 0.0127 | 0.0428 | 0.0003 | 0.0207 |
Punctate Nuclear | 0.0022 | 0.0002 | 0.0009 | 0.0028 | 0.0009 | 0.0005 | 0.0071 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0004 | 0.0002 | 0 | 0.0001 | 0 | 0.0002 |
Vacuole | 0.0037 | 0.0081 | 0.0037 | 0.0013 | 0.0005 | 0.003 | 0.0127 | 0.0025 | 0.0012 | 0.0602 | 0.0023 | 0.0018 | 0.0085 | 0.0015 | 0.0016 | 0.0014 | 0.0113 | 0.004 |
Vacuole Periphery | 0.0029 | 0.0105 | 0.0064 | 0.0021 | 0.0007 | 0.0052 | 0.0202 | 0.0042 | 0.0026 | 0.0217 | 0.0036 | 0.0052 | 0.0085 | 0.0037 | 0.0039 | 0.0038 | 0.0339 | 0.0078 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 84.3422 | 85.5015 | 72.6743 | 63.3533 | 58.6224 | 51.2725 | 80.9491 | 77.1788 | 65.2063 | 73.5477 |
Translational Efficiency | 0.7321 | 0.7659 | 0.6851 | 0.6702 | 0.7225 | 0.8854 | 0.7492 | 0.6019 | 0.6265 | 0.6263 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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