Standard name | |
---|---|
Human Ortholog | |
Description | Alpha-1,2-mannosyltransferase; responsible for addition of the second alpha-1,2-linked mannose of the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 10 | – | 22 | 20 | 20 | 37 | – | 19 | 26 | 14 | 15 | – | 3 | 1 | 7 | 4 | 7 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud | 1 | 0 | – | 3 | 3 | 6 | 24 | – | 17 | 23 | 16 | 12 | – | 0 | 2 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | – | 0 | 0 | 0 | 2 | – | 1 | 0 | 4 | 2 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | – | 2 | 0 | 1 | 3 | – | 1 | 0 | 0 | 2 | – | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 13 | 33 | – | 8 | 15 | 20 | 22 | – | 7 | 21 | 10 | 15 | – | 5 | 51 | 28 | 41 | 23 | 1 | 2 | 0 | 1 | 0 | 2 |
Endoplasmic Reticulum | 5 | 8 | – | 2 | 7 | 7 | 3 | – | 3 | 2 | 3 | 6 | – | 2 | 1 | 10 | 8 | 12 | 1 | 0 | 0 | 0 | 0 | 0 |
Endosome | 19 | 9 | – | 66 | 62 | 51 | 126 | – | 42 | 67 | 19 | 55 | – | 17 | 42 | 25 | 27 | 27 | 47 | 31 | 12 | 2 | 16 | 20 |
Golgi | 11 | 19 | – | 53 | 50 | 40 | 75 | – | 33 | 67 | 13 | 23 | – | 22 | 3 | 28 | 54 | 42 | 43 | 47 | 5 | 7 | 34 | 28 |
Mitochondria | 65 | 9 | – | 25 | 9 | 50 | 74 | – | 110 | 86 | 131 | 97 | – | 8 | 3 | 15 | 13 | 15 | 9 | 6 | 2 | 4 | 7 | 6 |
Nucleus | 1 | 0 | – | 2 | 0 | 0 | 2 | – | 1 | 1 | 0 | 1 | – | 2 | 1 | 9 | 3 | 3 | 0 | 0 | 0 | 1 | 1 | 2 |
Nuclear Periphery | 0 | 0 | – | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 7 | 0 | – | 2 | 1 | 9 | 9 | – | 14 | 8 | 10 | 12 | – | 2 | 0 | 1 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | – | 1 | 7 | 4 | 11 | – | 1 | 11 | 2 | 4 | – | 0 | 1 | 0 | 0 | 0 | 2 | 1 | 1 | 0 | 0 | 0 |
SpindlePole | 1 | 2 | – | 16 | 16 | 13 | 40 | – | 19 | 37 | 7 | 18 | – | 4 | 4 | 1 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 56 | 35 | – | 27 | 30 | 20 | 26 | – | 18 | 24 | 9 | 22 | – | 20 | 48 | 233 | 222 | 168 | 41 | 36 | 24 | 7 | 23 | 14 |
Unique Cell Count | 130 | 98 | 157 | 164 | 184 | 322 | 197 | 267 | 176 | 217 | 63 | 124 | 283 | 289 | 223 | 148 | 129 | 51 | 25 | 85 | 80 | |||
Labelled Cell Count | 179 | 126 | 229 | 220 | 241 | 455 | 286 | 373 | 238 | 284 | 85 | 157 | 358 | 376 | 306 | 148 | 129 | 51 | 25 | 85 | 80 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.6 | 8.5 | – | 8.9 | 10.2 | 8.6 | 8.8 | – | 7.3 | 7.9 | 6.7 | 7.3 | – | 9.0 | 7.8 | 12.4 | 12.4 | 12.5 | – | 8.3 | 8.7 |
Std Deviation (1e-4) | 1.1 | 1.3 | – | 1.3 | 1.7 | 1.3 | 1.4 | – | 1.0 | 1.1 | 1.1 | 1.1 | – | 1.3 | 1.6 | 2.7 | 2.7 | 2.8 | – | 1.7 | 1.2 |
Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.7153 | 6.0023 | 5.2128 | 5.3189 | 4.3975 | 5.2804 | 4.2467 | 6.3393 | 6.0397 | 5.9792 | 4.5018 | 5.9706 | 3.2739 | 5.1398 | 4.8013 | 4.0406 | 3.9514 | 4.4959 |
Actin | 0.0082 | 0.0086 | 0.0096 | 0.0052 | 0.011 | 0.0062 | 0.0161 | 0.0017 | 0.008 | 0.0001 | 0.0003 | 0.0102 | 0.0385 | 0.005 | 0.011 | 0.0013 | 0.0431 | 0.0023 |
Bud | 0.0005 | 0.0032 | 0.0002 | 0.0003 | 0.0004 | 0.0001 | 0.0004 | 0.0001 | 0.0003 | 0 | 0.0002 | 0.0001 | 0.0003 | 0.0086 | 0.0001 | 0.0001 | 0.0002 | 0 |
Bud Neck | 0.0003 | 0.0002 | 0.0008 | 0.0004 | 0.0008 | 0.0003 | 0.0006 | 0.0001 | 0.0015 | 0.0001 | 0.0002 | 0.0003 | 0.0005 | 0.0002 | 0.0002 | 0.0002 | 0.0005 | 0.0002 |
Bud Periphery | 0.0002 | 0.0014 | 0.0003 | 0.0006 | 0.0003 | 0.0001 | 0.0005 | 0.0001 | 0.0004 | 0 | 0.0002 | 0.0002 | 0.0004 | 0.0033 | 0.0001 | 0.0001 | 0.0005 | 0 |
Bud Site | 0.0006 | 0.0017 | 0.0006 | 0.0005 | 0.0007 | 0.0001 | 0.0029 | 0.0002 | 0.0012 | 0 | 0.0046 | 0.0002 | 0.0011 | 0.0077 | 0.0004 | 0.0003 | 0.001 | 0.0001 |
Cell Periphery | 0.0003 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0 | 0 | 0.0002 | 0 |
Cytoplasm | 0.0037 | 0.0026 | 0.0015 | 0.0008 | 0.0095 | 0.001 | 0.008 | 0.0129 | 0.0024 | 0.0003 | 0.0011 | 0.0009 | 0.0038 | 0.0035 | 0.0028 | 0.0022 | 0.0015 | 0.0014 |
Cytoplasmic Foci | 0.0812 | 0.1032 | 0.0753 | 0.0574 | 0.0972 | 0.0593 | 0.0786 | 0.0903 | 0.0646 | 0.0349 | 0.0597 | 0.0439 | 0.0798 | 0.0824 | 0.0826 | 0.0786 | 0.0654 | 0.0543 |
Eisosomes | 0.0002 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0 | 0.0002 | 0.0004 | 0 | 0.0001 | 0 | 0.0016 | 0 |
Endoplasmic Reticulum | 0.0011 | 0.0007 | 0.0012 | 0.0007 | 0.002 | 0.0011 | 0.0025 | 0.0015 | 0.0011 | 0.0004 | 0.001 | 0.001 | 0.0024 | 0.0003 | 0.001 | 0.0024 | 0.0024 | 0.0005 |
Endosome | 0.3145 | 0.3382 | 0.3038 | 0.3031 | 0.3007 | 0.268 | 0.3189 | 0.3774 | 0.3134 | 0.3682 | 0.3353 | 0.3524 | 0.2931 | 0.2604 | 0.2443 | 0.3057 | 0.3691 | 0.2913 |
Golgi | 0.4944 | 0.4815 | 0.5323 | 0.5643 | 0.4739 | 0.5885 | 0.4393 | 0.4549 | 0.5381 | 0.5512 | 0.4844 | 0.4976 | 0.4794 | 0.6018 | 0.6133 | 0.4882 | 0.4637 | 0.606 |
Lipid Particles | 0.0264 | 0.0177 | 0.0179 | 0.0168 | 0.0247 | 0.0151 | 0.0388 | 0.0138 | 0.018 | 0.0107 | 0.0453 | 0.0275 | 0.0255 | 0.0081 | 0.0055 | 0.0198 | 0.015 | 0.024 |
Mitochondria | 0.0227 | 0.0133 | 0.0241 | 0.0233 | 0.0127 | 0.0264 | 0.027 | 0.0096 | 0.0177 | 0.0136 | 0.0229 | 0.0268 | 0.0294 | 0.0088 | 0.01 | 0.0041 | 0.0079 | 0.0072 |
None | 0.0081 | 0.0008 | 0.0008 | 0.0016 | 0.002 | 0.0001 | 0.0075 | 0.0004 | 0.0006 | 0 | 0.0003 | 0.0002 | 0.0033 | 0.0002 | 0.0036 | 0.0016 | 0.0016 | 0 |
Nuclear Periphery | 0.0042 | 0.0004 | 0.0013 | 0.0006 | 0.0128 | 0.0033 | 0.0111 | 0.0007 | 0.0015 | 0.0002 | 0.001 | 0.0024 | 0.0048 | 0.0001 | 0.0011 | 0.0042 | 0.0046 | 0.0002 |
Nucleolus | 0.0024 | 0.0016 | 0.0003 | 0.0002 | 0.0008 | 0.0021 | 0.0013 | 0 | 0.002 | 0 | 0.0005 | 0.0001 | 0.0062 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0 |
Nucleus | 0.0021 | 0.0004 | 0.0004 | 0.0002 | 0.0099 | 0.0041 | 0.0082 | 0.0002 | 0.0012 | 0 | 0.0002 | 0.0001 | 0.0015 | 0.0001 | 0.0002 | 0.0005 | 0.0003 | 0 |
Peroxisomes | 0.0114 | 0.011 | 0.014 | 0.0058 | 0.0188 | 0.0048 | 0.0175 | 0.0164 | 0.0149 | 0.0008 | 0.0076 | 0.0041 | 0.0194 | 0.0041 | 0.0116 | 0.0383 | 0.0095 | 0.0035 |
Punctate Nuclear | 0.0032 | 0.0002 | 0.0026 | 0.0005 | 0.0066 | 0.0009 | 0.0048 | 0.0001 | 0.0017 | 0 | 0.0007 | 0.0002 | 0.0023 | 0.0001 | 0.0028 | 0.0033 | 0.0021 | 0.0001 |
Vacuole | 0.0074 | 0.006 | 0.0059 | 0.0096 | 0.0054 | 0.0076 | 0.0063 | 0.0109 | 0.0052 | 0.0076 | 0.0154 | 0.0114 | 0.0037 | 0.0023 | 0.0034 | 0.0179 | 0.0033 | 0.0033 |
Vacuole Periphery | 0.007 | 0.0072 | 0.0069 | 0.0078 | 0.0097 | 0.0109 | 0.0091 | 0.0086 | 0.0061 | 0.0116 | 0.019 | 0.02 | 0.0042 | 0.0026 | 0.0054 | 0.0309 | 0.0064 | 0.0056 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 67.9837 | 57.6926 | 39.4853 | 42.8386 | 58.4918 | 54.4821 | 48.2804 | 41.4732 | 38.8079 | 58.3627 |
Translational Efficiency | 1.347 | 1.2222 | 1.2471 | 1.1307 | 1.1606 | 1.1309 | 1.1059 | 1.1275 | 1.0031 | 1.0204 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|