Standard name | |
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Human Ortholog | |
Description | Essential ATP-binding protein required for DNA replication; component of the pre-replicative complex (pre-RC) which requires ORC to associate with chromatin and is in turn required for Mcm2-7p DNA association; homologous to S. pombe Cdc18p; relocalizes from nucleus to cytoplasm upon DNA replication stress; degraded in response to plasma membrane stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2 | 0 | 0 | 3 | 1 |
Bud | 2 | 1 | 0 | 0 | 1 | 1 | 2 | 1 | 5 | 0 | 2 | 4 | 0 | 0 | 0 | – | 0 | 0 | 3 | 9 | 2 | 1 | 4 | 6 |
Bud Neck | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | – | 2 | 1 | 1 | 2 | 0 | 0 | 0 | 3 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 1 | 2 | 1 | 0 | 0 | 0 | – | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | – | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 1 |
Cytoplasm | 80 | 169 | 98 | 91 | 110 | 128 | 121 | 133 | 113 | 112 | 54 | 56 | 192 | 298 | 223 | – | 202 | 151 | 69 | 129 | 59 | 94 | 217 | 199 |
Endoplasmic Reticulum | 0 | 1 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 1 | 1 | 1 | 1 | 0 | – | 21 | 13 | 0 | 0 | 1 | 1 | 0 | 2 |
Endosome | 0 | 0 | 0 | 2 | 4 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 2 | 0 | – | 7 | 7 | 1 | 3 | 2 | 1 | 0 | 3 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 0 |
Mitochondria | 97 | 32 | 31 | 29 | 26 | 112 | 116 | 101 | 107 | 199 | 128 | 151 | 5 | 1 | 0 | – | 4 | 6 | 12 | 19 | 14 | 9 | 14 | 9 |
Nucleus | 63 | 75 | 27 | 8 | 3 | 14 | 8 | 12 | 7 | 12 | 11 | 9 | 11 | 1 | 0 | – | 50 | 62 | 72 | 98 | 32 | 21 | 1 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 1 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Nucleolus | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 1 | 4 | 2 | 2 | 1 | 1 | 2 | 1 | 5 | 1 | 0 | 0 | – | 0 | 3 | 7 | 24 | 11 | 7 | 1 | 4 |
Vac/Vac Membrane | 21 | 63 | 9 | 6 | 11 | 17 | 6 | 6 | 5 | 10 | 3 | 9 | 18 | 12 | 0 | – | 39 | 43 | 39 | 44 | 28 | 21 | 7 | 9 |
Unique Cell Count | 199 | 309 | 156 | 124 | 143 | 219 | 206 | 207 | 185 | 268 | 157 | 188 | 221 | 309 | 223 | 291 | 260 | 218 | 348 | 160 | 168 | 263 | 252 | |
Labelled Cell Count | 263 | 343 | 169 | 138 | 159 | 276 | 258 | 259 | 242 | 341 | 202 | 238 | 232 | 316 | 224 | 326 | 287 | 218 | 348 | 160 | 168 | 263 | 252 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.3 | 4.1 | 3.5 | 3.5 | 3.7 | 2.8 | 2.6 | 3.0 | 2.7 | 2.4 | 2.5 | 2.5 | 4.3 | 4.0 | 4.2 | 4.7 | 5.9 | 5.7 | 4.1 | 4.1 | 4.9 |
Std Deviation (1e-4) | 0.6 | 0.8 | 0.8 | 1.3 | 2.2 | 0.6 | 1.2 | 1.3 | 1.2 | 0.7 | 0.9 | 1.0 | 1.2 | 1.4 | 1.9 | 1.4 | 1.7 | 1.6 | 1.6 | 1.8 | 2.1 |
Intensity Change (Log2) | – | – | – | -0.01 | 0.06 | -0.35 | -0.43 | -0.25 | -0.38 | -0.55 | -0.47 | -0.53 | 0.27 | 0.19 | 0.24 | 0.4 | 0.74 | 0.69 | 0.22 | 0.22 | 0.46 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | 2.5 | 3.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.0646 | 0.0022 | 0.0006 | 0.0288 | 0.0002 | 0.0003 | 0.0397 | 0.0005 | 0.0057 | 0.0007 | 0.0433 | 0.0074 | 0.014 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0009 |
Bud | 0.0008 | 0.0101 | 0.0004 | 0.0003 | 0 | 0.002 | 0.0016 | 0.0068 | 0.0036 | 0.0023 | 0.0006 | 0.0024 | 0.001 | 0.0002 | 0.0014 | 0.0001 | 0.0094 | 0.0057 |
Bud Neck | 0.0149 | 0.0004 | 0.0005 | 0.0002 | 0.0001 | 0.0042 | 0.0018 | 0.0011 | 0.0006 | 0.0003 | 0.001 | 0.0028 | 0.0003 | 0.0003 | 0.0001 | 0.0024 | 0.0005 | 0.0045 |
Bud Periphery | 0.0009 | 0.0094 | 0.0006 | 0.0004 | 0 | 0.0035 | 0.0027 | 0.0063 | 0.0035 | 0.0065 | 0.0009 | 0.0085 | 0.001 | 0.0001 | 0.0019 | 0 | 0.0101 | 0.019 |
Bud Site | 0.0277 | 0.0112 | 0.0033 | 0.0024 | 0.0001 | 0.0012 | 0.0102 | 0.0127 | 0.0084 | 0.0007 | 0.0016 | 0.0004 | 0.0009 | 0.0013 | 0.0011 | 0 | 0.0007 | 0.0018 |
Cell Periphery | 0.0005 | 0.0009 | 0.0003 | 0.0001 | 0.0001 | 0.0004 | 0.0006 | 0.0007 | 0.0004 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0004 | 0.0003 | 0.0001 | 0.0002 | 0.0009 |
Cytoplasm | 0.0225 | 0.0722 | 0.0519 | 0.0337 | 0.0803 | 0.0733 | 0.0634 | 0.0643 | 0.0676 | 0.0414 | 0.0785 | 0.037 | 0.0249 | 0.0838 | 0.1254 | 0.2229 | 0.0418 | 0.0736 |
Cytoplasmic Foci | 0.0586 | 0.0018 | 0.0027 | 0.0075 | 0.004 | 0.0018 | 0.0221 | 0.0021 | 0.0037 | 0.0031 | 0.0459 | 0.0019 | 0.0219 | 0.0008 | 0.0019 | 0.001 | 0.0009 | 0.0035 |
Eisosomes | 0.0009 | 0.0003 | 0.0005 | 0.0005 | 0.0001 | 0.0001 | 0.0006 | 0.0003 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0013 | 0.0006 | 0.0004 | 0.0002 | 0.0002 |
Endoplasmic Reticulum | 0.0011 | 0.0039 | 0.0019 | 0.0008 | 0.003 | 0.0023 | 0.0063 | 0.0021 | 0.001 | 0.0006 | 0.0019 | 0.001 | 0.0421 | 0.0007 | 0.0037 | 0.0016 | 0.0006 | 0.0012 |
Endosome | 0.0174 | 0.0011 | 0.001 | 0.0018 | 0.0046 | 0.0019 | 0.0253 | 0.001 | 0.0006 | 0.0009 | 0.048 | 0.0017 | 0.6323 | 0.0001 | 0.0009 | 0.0006 | 0.0001 | 0.0018 |
Golgi | 0.0135 | 0.0001 | 0.0002 | 0.0037 | 0.0001 | 0.0001 | 0.0149 | 0.0001 | 0.0004 | 0.0001 | 0.0131 | 0.0011 | 0.0585 | 0 | 0.0001 | 0 | 0 | 0.0001 |
Lipid Particles | 0.0431 | 0.0003 | 0.0005 | 0.0046 | 0.001 | 0.0002 | 0.0586 | 0.0003 | 0.0005 | 0.0001 | 0.0227 | 0.0035 | 0.0108 | 0.0001 | 0.0003 | 0 | 0 | 0.0002 |
Mitochondria | 0.0066 | 0.0028 | 0.0264 | 0.0154 | 0.0004 | 0.0012 | 0.036 | 0.0015 | 0.0113 | 0.0029 | 0.011 | 0.0014 | 0.0319 | 0.0006 | 0.0007 | 0.0001 | 0.0008 | 0.0012 |
None | 0.1939 | 0.5802 | 0.7397 | 0.8725 | 0.8754 | 0.2608 | 0.2374 | 0.5994 | 0.7684 | 0.875 | 0.6358 | 0.2548 | 0.0096 | 0.7408 | 0.8323 | 0.7473 | 0.7317 | 0.3853 |
Nuclear Periphery | 0.0058 | 0.0053 | 0.0024 | 0.0002 | 0.0046 | 0.0043 | 0.0188 | 0.0081 | 0.0024 | 0.0031 | 0.0011 | 0.0049 | 0.0952 | 0.0011 | 0.0044 | 0.0023 | 0.0031 | 0.0045 |
Nucleolus | 0.0126 | 0.0032 | 0.0043 | 0.0001 | 0.0009 | 0.0128 | 0.0128 | 0.0085 | 0.0024 | 0.0022 | 0.0031 | 0.015 | 0.0004 | 0.0007 | 0.0009 | 0.0001 | 0.0022 | 0.0076 |
Nucleus | 0.4486 | 0.284 | 0.1561 | 0.0007 | 0.0191 | 0.6163 | 0.3495 | 0.2637 | 0.1006 | 0.0426 | 0.0738 | 0.635 | 0.0074 | 0.1664 | 0.0169 | 0.0188 | 0.1939 | 0.474 |
Peroxisomes | 0.0333 | 0.0002 | 0.0002 | 0.0257 | 0.0001 | 0.0001 | 0.0604 | 0.0002 | 0.0046 | 0.0008 | 0.0082 | 0.0075 | 0.0017 | 0 | 0.0001 | 0 | 0 | 0.0003 |
Punctate Nuclear | 0.0295 | 0.0023 | 0.005 | 0.0004 | 0.0036 | 0.0086 | 0.029 | 0.0142 | 0.0125 | 0.0156 | 0.004 | 0.011 | 0.0032 | 0.0003 | 0.0017 | 0.0012 | 0.0006 | 0.0088 |
Vacuole | 0.0025 | 0.0068 | 0.0014 | 0.0002 | 0.0021 | 0.0042 | 0.0062 | 0.0054 | 0.0012 | 0.0007 | 0.0034 | 0.002 | 0.015 | 0.0007 | 0.0043 | 0.0005 | 0.0023 | 0.0044 |
Vacuole Periphery | 0.0007 | 0.0012 | 0.0002 | 0.0001 | 0.0002 | 0.0006 | 0.0018 | 0.0007 | 0.0002 | 0.0003 | 0.0014 | 0.0005 | 0.0277 | 0.0001 | 0.0007 | 0.0001 | 0.0007 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 10.3757 | 13.4331 | 42.2452 | 65.4446 | 12.5248 | 18.5944 | 18.1096 | 23.5725 | 41.1295 | 25.565 |
Translational Efficiency | 0.5371 | 0.8821 | 0.7373 | 0.7065 | 0.8342 | 0.5687 | 0.5214 | 0.5109 | 0.931 | 0.5626 |
Dataset | Hit |
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Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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