Standard name
Human Ortholog
Description Subunit of the SF3a splicing factor complex; required for spliceosome assembly
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.1 0.18 0.1 0.11 0.12 0 0 0 0.05 0 0.05
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.29 0 0 0 0.08 0.11 0.09 0.55 0.31 0.35 0.34 0 0 0 0.05 0.08 0 0 0 0 0 0 0
Nucleus 0.93 0.89 0.95 0.95 0.93 0.93 0.89 0.86 0.84 0.84 0.85 0.85 0.91 0.89 0.8 0.85 0.82 0.84 0.91 0.89 0.88 0.81 0.8 0.66
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0.06 0.06 0.14
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0
Bud 0 1 0 0 0 0 4 1 5 2 6 6 0 2 0 1 0 0 2 3 3 1 3 6
Bud Neck 0 0 0 0 0 0 0 1 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 1 7 12 8 16 14 23 11 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2 11 14 12 12 14 19 30 16 21 10 8 11 33 56 19 18 22 0 5 4 7 13 14
Endoplasmic Reticulum 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 7 4 7 0 0 1 0 1 1
Endosome 1 0 3 0 0 3 3 7 0 2 0 0 0 0 1 0 1 0 3 3 2 1 2 4
Golgi 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 1
Mitochondria 8 74 1 6 5 42 87 65 272 201 202 188 3 6 1 10 13 4 0 3 3 1 3 8
Nucleus 174 227 401 275 315 483 690 622 416 543 492 470 116 293 250 160 139 152 176 225 396 115 248 179
Nuclear Periphery 1 0 8 2 3 10 19 4 4 10 2 4 0 2 0 2 3 3 0 0 2 0 3 1
Nucleolus 9 4 1 2 3 7 13 25 10 24 25 15 1 1 0 3 0 2 1 1 4 3 6 8
Peroxisomes 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 1 0 5 6 10 2 1 5 5 0 1 4 1 0 0 0 0 2 0 2 3
Vac/Vac Membrane 2 5 5 2 7 8 13 30 3 9 7 10 0 6 2 4 10 7 7 7 17 9 20 38
Unique Cell Count 187 256 424 290 337 522 772 727 498 648 576 556 127 330 312 188 169 182 193 254 453 142 310 273
Labelled Cell Count 197 322 436 300 346 579 867 804 745 827 774 718 132 344 315 207 188 197 193 254 453 142 310 273


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.3 4.6 7.0 6.2 6.8 5.3 5.3 5.6 4.1 4.7 4.4 4.8 7.4 6.4 6.0 6.5 7.4 8.3 9.6 9.2 9.4
Std Deviation (1e-4) 1.1 0.7 1.6 1.5 1.8 1.2 1.1 1.6 1.1 1.2 0.9 1.9 9.3 3.3 1.5 1.8 1.9 2.4 15.0 11.4 10.7
Intensity Change (Log2) – – – -0.16 -0.04 -0.4 -0.38 -0.31 -0.76 -0.57 -0.66 -0.55 0.09 -0.13 -0.21 -0.11 0.08 0.25 0.46 0.4 0.44


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.6423 4.032 3.7039 3.209 3.4313 3.6814 4.6277 4.8623 4.5201 5.0963 4.3064 4.8528 1.4533 1.8441 1.6926 1.6447 1.3075 1.6064
Actin 0.0076 0 0.003 0 0.0004 0.0021 0.0472 0 0.0137 0 0.0001 0.0038 0.0009 0 0.0002 0.0034 0 0
Bud 0.0002 0 0.0001 0 0 0 0.0007 0 0.0004 0 0 0.0001 0.0002 0 0.0001 0.0001 0 0
Bud Neck 0.0043 0.0001 0.001 0 0.0004 0.0011 0.0014 0.0001 0.0012 0 0.0005 0.0012 0.0011 0 0.0002 0.0202 0.0003 0.0005
Bud Periphery 0.0006 0 0.0003 0 0 0 0.0021 0 0.0008 0 0 0.0002 0.0005 0 0.0001 0.0001 0 0
Bud Site 0.0031 0 0.0022 0 0.0001 0.0001 0.0022 0.0001 0.0027 0 0 0.0004 0.0006 0 0.0001 0.0014 0 0
Cell Periphery 0.0004 0 0.0001 0 0 0 0.0006 0 0.0002 0 0 0.0001 0.0001 0 0 0.0001 0 0
Cytoplasm 0.0014 0.0001 0.0007 0 0.0017 0.0001 0.0027 0 0.0012 0.0001 0.0004 0.0001 0.0021 0.0001 0.0004 0.0002 0.0003 0
Cytoplasmic Foci 0.0144 0 0.0014 0 0.002 0.0005 0.0071 0 0.0029 0 0 0.002 0.0011 0 0 0.0019 0 0
Eisosomes 0.0004 0 0 0 0 0 0.0017 0 0.0001 0 0 0 0 0 0 0.0001 0 0
Endoplasmic Reticulum 0.0079 0 0.0002 0 0.0009 0.0001 0.0026 0 0.0015 0 0.0002 0.0001 0.0011 0 0.0004 0 0 0
Endosome 0.0105 0 0.0009 0 0.0089 0.001 0.016 0 0.0075 0 0.0004 0.0044 0.0031 0 0.001 0.0003 0 0
Golgi 0.0038 0 0.0011 0 0.0016 0.0008 0.0067 0 0.0029 0 0 0.007 0.0003 0 0 0.0006 0 0
Lipid Particles 0.0193 0 0.0008 0 0.0261 0.0031 0.0118 0 0.0094 0 0 0.0032 0.0007 0 0 0.0005 0 0
Mitochondria 0.002 0.0001 0.0003 0.0001 0.0033 0.0011 0.0123 0.0002 0.0083 0.0003 0.0003 0.0181 0.0014 0 0.0007 0.0003 0.0001 0
None 0.0072 0 0.0009 0 0.0011 0.0001 0.0117 0 0.0003 0.0001 0.0001 0.0001 0.0008 0 0.0001 0.0001 0.0001 0
Nuclear Periphery 0.006 0.0005 0.0018 0.0006 0.0296 0.0004 0.0153 0.0022 0.0047 0.0032 0.0081 0.0003 0.0057 0.0007 0.0052 0.0003 0.0017 0.0002
Nucleolus 0.0086 0.0074 0.0114 0.0038 0.0149 0.0181 0.0123 0.0111 0.0042 0.0075 0.0214 0.02 0.0112 0.0045 0.0047 0.0034 0.0195 0.0198
Nucleus 0.8602 0.9832 0.9572 0.9953 0.8972 0.9692 0.8226 0.986 0.929 0.9877 0.9664 0.9354 0.9607 0.9942 0.9857 0.9639 0.9726 0.9764
Peroxisomes 0.0263 0 0.0006 0 0.003 0.0009 0.0099 0 0.0057 0 0 0.002 0.0001 0 0 0.0028 0 0
Punctate Nuclear 0.0135 0.0086 0.0154 0.0001 0.0068 0.0011 0.0034 0.0002 0.0012 0.0007 0.0013 0.001 0.0062 0.0003 0.0003 0.0003 0.0052 0.0029
Vacuole 0.0015 0 0.0005 0 0.001 0.0001 0.0069 0 0.0014 0 0.0005 0.0003 0.0017 0 0.0003 0 0 0
Vacuole Periphery 0.0007 0 0.0001 0 0.001 0.0001 0.0029 0 0.0007 0.0001 0.0004 0.0002 0.0006 0 0.0003 0 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 3.2723 5.8504 13.0237 9.6427 11.0308 5.2327 4.1407 13.9736 9.5865 9.4639
Translational Efficiency 6.9676 3.6763 1.3098 1.5341 1.6319 4.4217 5.5355 1.4157 2.0653 1.944

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of the SF3a splicing factor complex; required for spliceosome assembly
Localization
Cell Percentages nucleus (100%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Prp21

Prp21


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Prp21-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available