Standard name | |
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Human Ortholog | |
Description | AP-1 accessory protein; colocalizes with clathrin to the late-Golgi apparatus; involved in TGN-endosome transport; physically interacts with AP-1; similar to the mammalian p200; may interact with ribosomes; YJL207C is a non-essential gene |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 7 | 14 | 10 | 5 | 4 | 6 | 4 | 8 | 1 | 5 | 2 | 0 | 5 | 2 | 2 | 16 | 12 | 15 | 9 | 37 | 37 | 22 | 22 | 26 |
Bud | 5 | 10 | 61 | 22 | 72 | 84 | 81 | 102 | 61 | 46 | 64 | 78 | 7 | 6 | 23 | 5 | 5 | 4 | 0 | 3 | 2 | 1 | 0 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 2 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 |
Bud Site | 0 | 1 | 7 | 9 | 7 | 35 | 38 | 33 | 28 | 29 | 26 | 50 | 0 | 0 | 5 | 2 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 2 | 0 | 1 | 4 | 2 | 3 | 1 | 4 | 2 | 6 | 0 | 0 | 0 | 2 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 15 | 11 | 6 | 16 | 21 | 28 | 38 | 18 | 22 | 18 | 27 | 5 | 7 | 7 | 13 | 20 | 23 | 0 | 0 | 1 | 0 | 0 | 1 |
Endoplasmic Reticulum | 0 | 9 | 7 | 1 | 3 | 1 | 4 | 1 | 0 | 3 | 3 | 3 | 3 | 0 | 1 | 5 | 9 | 2 | 0 | 2 | 1 | 0 | 0 | 1 |
Endosome | 15 | 32 | 80 | 7 | 58 | 26 | 24 | 25 | 0 | 3 | 2 | 4 | 25 | 50 | 26 | 33 | 24 | 35 | 31 | 108 | 139 | 60 | 36 | 40 |
Golgi | 30 | 38 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 9 | 11 | 3 | 59 | 65 | 58 | 47 | 120 | 131 | 95 | 80 | 124 |
Mitochondria | 58 | 251 | 368 | 216 | 303 | 550 | 529 | 566 | 322 | 416 | 373 | 510 | 101 | 79 | 163 | 14 | 16 | 9 | 9 | 47 | 65 | 18 | 9 | 15 |
Nucleus | 0 | 0 | 2 | 1 | 2 | 2 | 6 | 11 | 5 | 3 | 5 | 5 | 0 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 8 | 1 | 1 | 3 | 4 | 6 | 3 | 0 | 7 | 3 | 2 | 2 | 2 | 1 | 1 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 4 | 9 | 6 | 3 | 9 | 16 | 22 | 44 | 26 | 15 | 12 | 22 | 7 | 8 | 8 | 3 | 1 | 4 | 0 | 1 | 0 | 0 | 0 | 0 |
Peroxisomes | 9 | 18 | 84 | 24 | 148 | 94 | 96 | 72 | 40 | 37 | 35 | 59 | 30 | 48 | 21 | 4 | 3 | 2 | 24 | 46 | 138 | 32 | 39 | 45 |
SpindlePole | 8 | 10 | 52 | 14 | 61 | 100 | 86 | 136 | 65 | 50 | 62 | 96 | 8 | 15 | 8 | 15 | 17 | 17 | 6 | 6 | 9 | 4 | 1 | 3 |
Vac/Vac Membrane | 6 | 21 | 17 | 2 | 4 | 6 | 5 | 12 | 2 | 5 | 4 | 6 | 5 | 9 | 6 | 7 | 11 | 9 | 2 | 11 | 2 | 4 | 2 | 0 |
Unique Cell Count | 100 | 328 | 496 | 240 | 460 | 647 | 653 | 734 | 384 | 487 | 431 | 608 | 156 | 169 | 214 | 140 | 144 | 143 | 133 | 390 | 537 | 247 | 197 | 264 |
Labelled Cell Count | 143 | 437 | 711 | 311 | 692 | 949 | 934 | 1056 | 572 | 645 | 611 | 869 | 207 | 238 | 274 | 180 | 187 | 185 | 133 | 390 | 537 | 247 | 197 | 264 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.2 | 6.8 | 5.6 | 4.9 | 5.2 | 4.5 | 4.3 | 4.3 | 3.7 | 3.8 | 3.6 | 3.6 | 7.2 | 7.4 | 6.4 | 9.6 | 10.1 | 10.1 | 7.8 | 8.2 | 8.8 |
Std Deviation (1e-4) | 1.3 | 1.0 | 1.0 | 0.8 | 1.4 | 0.9 | 1.0 | 1.1 | 1.2 | 0.8 | 0.8 | 0.9 | 1.3 | 1.6 | 1.2 | 1.7 | 1.8 | 1.9 | 1.2 | 1.3 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.19 | -0.11 | -0.33 | -0.38 | -0.38 | -0.58 | -0.58 | -0.64 | -0.65 | 0.36 | 0.4 | 0.19 | 0.77 | 0.85 | 0.84 | 0.48 | 0.56 | 0.64 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | -1.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5.0 | 0 | 4.6 |
Bud | -1.3 | 1.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -2.8 | -3.3 | -0.6 | -3.0 | -3.1 | -3.3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 2.0 | 0.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.2 | 1.2 | 1.0 | 1.9 | 2.6 | 2.0 | 2.0 | 1.7 | 2.0 | 0 | 0 | 0.8 | 3.9 | 5.7 | 6.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | -5.2 | -1.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.8 | -1.4 | 2.0 | 0.2 | 2.3 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 14.6 | 15.3 | 14.3 |
Mitochondria | 5.0 | -2.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -2.3 | -6.6 | 0.6 | -13.7 | -13.6 | -14.5 |
Nucleus | 0 | 0 | 0 | 0 | 1.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | -2.5 | 5.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.7 | 3.2 | -2.5 | -4.3 | -4.6 | -4.8 |
SpindlePole | -2.1 | 1.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -2.0 | -0.6 | -3.0 | 0.1 | 0.5 | 0.5 |
Vacuole | -2.1 | -2.7 | -3.0 | -3.3 | -2.0 | -2.9 | -2.5 | -2.6 | -2.8 | -0.1 | 1.1 | -0.4 | 0.9 | 2.2 | 1.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.9953 | 5.0531 | 4.5957 | 4.2541 | 4.2414 | 4.8671 | 2.2178 | 3.3986 | 3.4125 | 3.3499 | 3.0729 | 3.5105 | 7.1128 | 7.8067 | 7.7506 | 7.0148 | 7.2776 | 7.5308 |
Actin | 0.0682 | 0.0338 | 0.0672 | 0.0337 | 0.0062 | 0.0499 | 0.0291 | 0.0122 | 0.0371 | 0.0055 | 0.0265 | 0.0222 | 0.0421 | 0.0766 | 0.0528 | 0.0174 | 0.0239 | 0.051 |
Bud | 0.0005 | 0.0013 | 0.0009 | 0.0006 | 0.0004 | 0.0002 | 0.0005 | 0.0008 | 0.0005 | 0.0005 | 0.004 | 0.0002 | 0.0003 | 0.0012 | 0.0007 | 0.0001 | 0.0002 | 0.0001 |
Bud Neck | 0.0023 | 0.0016 | 0.0022 | 0.0119 | 0.0004 | 0.0016 | 0.0022 | 0.0009 | 0.0008 | 0.0009 | 0.0006 | 0.0014 | 0.0024 | 0.0009 | 0.0008 | 0.0002 | 0.0007 | 0.0007 |
Bud Periphery | 0.0005 | 0.001 | 0.001 | 0.0006 | 0.0015 | 0.0002 | 0.0005 | 0.0005 | 0.0003 | 0.0003 | 0.0012 | 0.0003 | 0.0003 | 0.0007 | 0.0005 | 0.0001 | 0.0002 | 0.0001 |
Bud Site | 0.003 | 0.0258 | 0.0061 | 0.0058 | 0.0023 | 0.0006 | 0.0034 | 0.0066 | 0.0015 | 0.0025 | 0.0011 | 0.0006 | 0.002 | 0.0122 | 0.0016 | 0.0002 | 0.0007 | 0.0003 |
Cell Periphery | 0.0002 | 0.0002 | 0.0002 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Cytoplasm | 0.0021 | 0.0069 | 0.0046 | 0.0016 | 0.0008 | 0.0009 | 0.0036 | 0.0036 | 0.0028 | 0.002 | 0.0023 | 0.0011 | 0.004 | 0.0063 | 0.0059 | 0.0007 | 0.0009 | 0.0008 |
Cytoplasmic Foci | 0.1662 | 0.1936 | 0.1468 | 0.1808 | 0.1524 | 0.1298 | 0.1518 | 0.2079 | 0.1585 | 0.1826 | 0.188 | 0.1463 | 0.1714 | 0.1835 | 0.1442 | 0.1856 | 0.1524 | 0.1434 |
Eisosomes | 0.001 | 0.0001 | 0.0002 | 0.0002 | 0.0007 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0007 | 0.0005 | 0.0012 | 0.0004 | 0.0009 | 0.0002 | 0.0009 | 0.0003 | 0.0008 | 0.0013 | 0.0002 | 0.0003 | 0.001 | 0.0016 | 0.0009 | 0.0001 | 0.0001 | 0.0001 |
Endosome | 0.1362 | 0.1716 | 0.141 | 0.2099 | 0.1849 | 0.1094 | 0.2921 | 0.2469 | 0.2086 | 0.2108 | 0.268 | 0.1412 | 0.1884 | 0.1549 | 0.1491 | 0.153 | 0.1271 | 0.1046 |
Golgi | 0.1873 | 0.2584 | 0.3104 | 0.3211 | 0.3161 | 0.343 | 0.3081 | 0.3562 | 0.4224 | 0.4338 | 0.349 | 0.4797 | 0.3589 | 0.3379 | 0.368 | 0.5113 | 0.4783 | 0.3824 |
Lipid Particles | 0.0775 | 0.055 | 0.0445 | 0.0548 | 0.161 | 0.1057 | 0.0479 | 0.0285 | 0.0266 | 0.0368 | 0.0212 | 0.0279 | 0.0462 | 0.0144 | 0.0352 | 0.0204 | 0.0077 | 0.0369 |
Mitochondria | 0.0266 | 0.0189 | 0.0443 | 0.017 | 0.0456 | 0.0171 | 0.0254 | 0.0189 | 0.0198 | 0.0131 | 0.0473 | 0.0273 | 0.0204 | 0.0178 | 0.0276 | 0.0035 | 0.0132 | 0.0123 |
None | 0.0018 | 0.0002 | 0.0028 | 0.0001 | 0.0023 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0004 | 0.0015 | 0.0001 | 0.0029 | 0.0002 | 0.0003 | 0 | 0.0001 | 0.0001 |
Nuclear Periphery | 0.0108 | 0.0002 | 0.0028 | 0.0001 | 0.0009 | 0.0001 | 0.005 | 0.0009 | 0.0008 | 0.0046 | 0.0001 | 0.0001 | 0.0008 | 0.0006 | 0.0002 | 0 | 0 | 0 |
Nucleolus | 0.0004 | 0.0002 | 0.0011 | 0.0002 | 0.0005 | 0.0001 | 0.0003 | 0.0008 | 0.0001 | 0.0001 | 0.0033 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0 |
Nucleus | 0.0025 | 0.0002 | 0.0056 | 0.0001 | 0.0005 | 0.0001 | 0.0169 | 0.0003 | 0.0005 | 0.0004 | 0.0009 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 | 0 |
Peroxisomes | 0.3059 | 0.2255 | 0.2104 | 0.1562 | 0.1188 | 0.2383 | 0.1052 | 0.0998 | 0.1163 | 0.0996 | 0.0768 | 0.1479 | 0.1556 | 0.1882 | 0.2098 | 0.1065 | 0.1932 | 0.266 |
Punctate Nuclear | 0.004 | 0.001 | 0.0036 | 0.0006 | 0.0008 | 0.0011 | 0.0009 | 0.0015 | 0.0003 | 0.0007 | 0.0003 | 0.0003 | 0.0012 | 0.0005 | 0.0003 | 0.0001 | 0.0002 | 0.0003 |
Vacuole | 0.0014 | 0.003 | 0.0019 | 0.0023 | 0.0016 | 0.0007 | 0.0034 | 0.0099 | 0.0011 | 0.0018 | 0.0039 | 0.0017 | 0.0009 | 0.0014 | 0.0009 | 0.0002 | 0.0004 | 0.0003 |
Vacuole Periphery | 0.0011 | 0.001 | 0.0013 | 0.0017 | 0.0013 | 0.0006 | 0.0024 | 0.0032 | 0.001 | 0.0022 | 0.0033 | 0.0011 | 0.0009 | 0.0007 | 0.0008 | 0.0006 | 0.0004 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 31.6693 | 24.8022 | 23.0054 | 32.4534 | 30.2925 | 23.5336 | 32.5842 | 32.7377 | 26.7957 | 35.3431 |
Translational Efficiency | 0.4346 | 0.4642 | 0.4411 | 0.3154 | 0.3203 | 0.4525 | 0.3895 | 0.2626 | 0.3288 | 0.3334 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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