Standard name
Human Ortholog
Description AP-1 accessory protein; colocalizes with clathrin to the late-Golgi apparatus; involved in TGN-endosome transport; physically interacts with AP-1; similar to the mammalian p200; may interact with ribosomes; YJL207C is a non-essential gene

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.08 0.1 0.07 0.1 0.07 0.09 0.11 0.1
Bud 0.05 0 0.12 0.09 0.16 0.13 0.12 0.14 0.16 0.09 0.15 0.13 0 0 0.11 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0.05 0.06 0 0.07 0.06 0.06 0.08 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.05 0 0 0 0 0 0.05 0.05 0.05 0 0 0 0 0 0.09 0.14 0.16 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Endosome 0.15 0.1 0.16 0 0.13 0 0 0 0 0 0 0 0.16 0.3 0.12 0.24 0.17 0.24 0.23 0.28 0.26 0.25 0.19 0.15
Golgi 0.3 0.12 0 0 0 0 0 0 0 0 0 0 0.06 0.07 0 0.42 0.45 0.41 0.36 0.31 0.25 0.39 0.41 0.47
Mitochondria 0.58 0.77 0.74 0.9 0.66 0.85 0.81 0.77 0.84 0.85 0.87 0.84 0.65 0.47 0.76 0.1 0.11 0.06 0.07 0.12 0.12 0.07 0.05 0.06
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0.06 0.07 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.09 0.05 0.17 0.1 0.32 0.15 0.15 0.1 0.1 0.08 0.08 0.1 0.19 0.28 0.1 0 0 0 0.18 0.12 0.26 0.13 0.2 0.17
SpindlePole 0.08 0 0.1 0.06 0.13 0.15 0.13 0.19 0.17 0.1 0.14 0.16 0.05 0.09 0 0.11 0.12 0.12 0.05 0 0 0 0 0
Vac/Vac Membrane 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0.08 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 7 14 10 5 4 6 4 8 1 5 2 0 5 2 2 16 12 15 9 37 37 22 22 26
Bud 5 10 61 22 72 84 81 102 61 46 64 78 7 6 23 5 5 4 0 3 2 1 0 2
Bud Neck 0 0 0 0 1 0 3 2 3 0 0 1 0 0 0 0 0 0 0 0 1 1 0 0
Bud Site 0 1 7 9 7 35 38 33 28 29 26 50 0 0 5 2 0 0
Cell Periphery 0 1 2 0 1 4 2 3 1 4 2 6 0 0 0 2 2 3 0 0 0 0 0 0
Cytoplasm 0 15 11 6 16 21 28 38 18 22 18 27 5 7 7 13 20 23 0 0 1 0 0 1
Endoplasmic Reticulum 0 9 7 1 3 1 4 1 0 3 3 3 3 0 1 5 9 2 0 2 1 0 0 1
Endosome 15 32 80 7 58 26 24 25 0 3 2 4 25 50 26 33 24 35 31 108 139 60 36 40
Golgi 30 38 3 0 0 0 0 0 0 0 0 0 9 11 3 59 65 58 47 120 131 95 80 124
Mitochondria 58 251 368 216 303 550 529 566 322 416 373 510 101 79 163 14 16 9 9 47 65 18 9 15
Nucleus 0 0 2 1 2 2 6 11 5 3 5 5 0 1 0 1 1 1 0 0 1 0 0 0
Nuclear Periphery 1 8 1 1 3 4 6 3 0 7 3 2 2 2 1 1 1 3 0 0 0 0 0 0
Nucleolus 4 9 6 3 9 16 22 44 26 15 12 22 7 8 8 3 1 4 0 1 0 0 0 0
Peroxisomes 9 18 84 24 148 94 96 72 40 37 35 59 30 48 21 4 3 2 24 46 138 32 39 45
SpindlePole 8 10 52 14 61 100 86 136 65 50 62 96 8 15 8 15 17 17 6 6 9 4 1 3
Vac/Vac Membrane 6 21 17 2 4 6 5 12 2 5 4 6 5 9 6 7 11 9 2 11 2 4 2 0
Unique Cell Count 100 328 496 240 460 647 653 734 384 487 431 608 156 169 214 140 144 143 133 390 537 247 197 264
Labelled Cell Count 143 437 711 311 692 949 934 1056 572 645 611 869 207 238 274 180 187 185 133 390 537 247 197 264


Late Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.2 6.8 5.6 4.9 5.2 4.5 4.3 4.3 3.7 3.8 3.6 3.6 7.2 7.4 6.4 9.6 10.1 10.1 7.8 8.2 8.8
Std Deviation (1e-4) 1.3 1.0 1.0 0.8 1.4 0.9 1.0 1.1 1.2 0.8 0.8 0.9 1.3 1.6 1.2 1.7 1.8 1.9 1.2 1.3 1.4
Intensity Change (Log2) -0.19 -0.11 -0.33 -0.38 -0.38 -0.58 -0.58 -0.64 -0.65 0.36 0.4 0.19 0.77 0.85 0.84 0.48 0.56 0.64


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 -1.5 0 0 0 0 0 0 0 0 0 0 5.0 0 4.6
Bud -1.3 1.5 0 0 0 0 0 0 0 -2.8 -3.3 -0.6 -3.0 -3.1 -3.3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 2.0 0.1 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.2 1.2 1.0 1.9 2.6 2.0 2.0 1.7 2.0 0 0 0.8 3.9 5.7 6.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome -5.2 -1.5 0 0 0 0 0 0 0 0 3.8 -1.4 2.0 0.2 2.3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 14.6 15.3 14.3
Mitochondria 5.0 -2.8 0 0 0 0 0 0 0 -2.3 -6.6 0.6 -13.7 -13.6 -14.5
Nucleus 0 0 0 0 1.8 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0.9 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes -2.5 5.5 0 0 0 0 0 0 0 0.7 3.2 -2.5 -4.3 -4.6 -4.8
SpindlePole -2.1 1.3 0 0 0 0 0 0 0 -2.0 -0.6 -3.0 0.1 0.5 0.5
Vacuole -2.1 -2.7 -3.0 -3.3 -2.0 -2.9 -2.5 -2.6 -2.8 -0.1 1.1 -0.4 0.9 2.2 1.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.9953 5.0531 4.5957 4.2541 4.2414 4.8671 2.2178 3.3986 3.4125 3.3499 3.0729 3.5105 7.1128 7.8067 7.7506 7.0148 7.2776 7.5308
Actin 0.0682 0.0338 0.0672 0.0337 0.0062 0.0499 0.0291 0.0122 0.0371 0.0055 0.0265 0.0222 0.0421 0.0766 0.0528 0.0174 0.0239 0.051
Bud 0.0005 0.0013 0.0009 0.0006 0.0004 0.0002 0.0005 0.0008 0.0005 0.0005 0.004 0.0002 0.0003 0.0012 0.0007 0.0001 0.0002 0.0001
Bud Neck 0.0023 0.0016 0.0022 0.0119 0.0004 0.0016 0.0022 0.0009 0.0008 0.0009 0.0006 0.0014 0.0024 0.0009 0.0008 0.0002 0.0007 0.0007
Bud Periphery 0.0005 0.001 0.001 0.0006 0.0015 0.0002 0.0005 0.0005 0.0003 0.0003 0.0012 0.0003 0.0003 0.0007 0.0005 0.0001 0.0002 0.0001
Bud Site 0.003 0.0258 0.0061 0.0058 0.0023 0.0006 0.0034 0.0066 0.0015 0.0025 0.0011 0.0006 0.002 0.0122 0.0016 0.0002 0.0007 0.0003
Cell Periphery 0.0002 0.0002 0.0002 0.0003 0.0003 0.0001 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0 0 0
Cytoplasm 0.0021 0.0069 0.0046 0.0016 0.0008 0.0009 0.0036 0.0036 0.0028 0.002 0.0023 0.0011 0.004 0.0063 0.0059 0.0007 0.0009 0.0008
Cytoplasmic Foci 0.1662 0.1936 0.1468 0.1808 0.1524 0.1298 0.1518 0.2079 0.1585 0.1826 0.188 0.1463 0.1714 0.1835 0.1442 0.1856 0.1524 0.1434
Eisosomes 0.001 0.0001 0.0002 0.0002 0.0007 0.0001 0.0001 0.0001 0 0 0.0001 0.0001 0.0001 0.0001 0.0001 0 0 0.0001
Endoplasmic Reticulum 0.0007 0.0005 0.0012 0.0004 0.0009 0.0002 0.0009 0.0003 0.0008 0.0013 0.0002 0.0003 0.001 0.0016 0.0009 0.0001 0.0001 0.0001
Endosome 0.1362 0.1716 0.141 0.2099 0.1849 0.1094 0.2921 0.2469 0.2086 0.2108 0.268 0.1412 0.1884 0.1549 0.1491 0.153 0.1271 0.1046
Golgi 0.1873 0.2584 0.3104 0.3211 0.3161 0.343 0.3081 0.3562 0.4224 0.4338 0.349 0.4797 0.3589 0.3379 0.368 0.5113 0.4783 0.3824
Lipid Particles 0.0775 0.055 0.0445 0.0548 0.161 0.1057 0.0479 0.0285 0.0266 0.0368 0.0212 0.0279 0.0462 0.0144 0.0352 0.0204 0.0077 0.0369
Mitochondria 0.0266 0.0189 0.0443 0.017 0.0456 0.0171 0.0254 0.0189 0.0198 0.0131 0.0473 0.0273 0.0204 0.0178 0.0276 0.0035 0.0132 0.0123
None 0.0018 0.0002 0.0028 0.0001 0.0023 0.0001 0.0002 0.0003 0.0001 0.0004 0.0015 0.0001 0.0029 0.0002 0.0003 0 0.0001 0.0001
Nuclear Periphery 0.0108 0.0002 0.0028 0.0001 0.0009 0.0001 0.005 0.0009 0.0008 0.0046 0.0001 0.0001 0.0008 0.0006 0.0002 0 0 0
Nucleolus 0.0004 0.0002 0.0011 0.0002 0.0005 0.0001 0.0003 0.0008 0.0001 0.0001 0.0033 0.0001 0.0001 0.0001 0 0 0.0001 0
Nucleus 0.0025 0.0002 0.0056 0.0001 0.0005 0.0001 0.0169 0.0003 0.0005 0.0004 0.0009 0.0001 0.0001 0.0002 0.0001 0 0 0
Peroxisomes 0.3059 0.2255 0.2104 0.1562 0.1188 0.2383 0.1052 0.0998 0.1163 0.0996 0.0768 0.1479 0.1556 0.1882 0.2098 0.1065 0.1932 0.266
Punctate Nuclear 0.004 0.001 0.0036 0.0006 0.0008 0.0011 0.0009 0.0015 0.0003 0.0007 0.0003 0.0003 0.0012 0.0005 0.0003 0.0001 0.0002 0.0003
Vacuole 0.0014 0.003 0.0019 0.0023 0.0016 0.0007 0.0034 0.0099 0.0011 0.0018 0.0039 0.0017 0.0009 0.0014 0.0009 0.0002 0.0004 0.0003
Vacuole Periphery 0.0011 0.001 0.0013 0.0017 0.0013 0.0006 0.0024 0.0032 0.001 0.0022 0.0033 0.0011 0.0009 0.0007 0.0008 0.0006 0.0004 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 31.6693 24.8022 23.0054 32.4534 30.2925 23.5336 32.5842 32.7377 26.7957 35.3431
Translational Efficiency 0.4346 0.4642 0.4411 0.3154 0.3203 0.4525 0.3895 0.2626 0.3288 0.3334

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description AP-1 accessory protein; colocalizes with clathrin to the late-Golgi apparatus; involved in TGN-endosome transport; physically interacts with AP-1; similar to the mammalian p200; may interact with ribosomes; YJL207C is a non-essential gene
Localization
Cell Percentages mitochondrion (12%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Laa1

Laa1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Laa1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available