Standard name
Human Ortholog
Description Peptidylprolyl-cis/trans-isomerase (PPIase); specific for phosphorylated S/T residues N-terminal to proline; regulates phosphorylation of RNAPII large subunit (Rpo21p) C-terminal domain (CTD) at Ser7; associates with phospho-Ser5 form of RNAPII in vivo; present along entire coding length of genes; represses initiation of CUTs; required for efficient termination of mRNA transcription, trimethylation of histone H3; human ortholog PIN1 can complement yeast null and ts mutants
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0.06
Bud Neck 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.06 – 0 0.05 0.05 0.06 0.09 – 0.05 0.06 0.06 0.05 0.1 0.24 0.32 0.11 0.13 0.18 0 0 0 0 0.05 0.08
Endoplasmic Reticulum 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0.05
Golgi 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.08 0.13 0 0 0 0 0 0
Mitochondria 0 – 0 0 0 0.08 0 – 0.09 0 0.08 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.5 – 0.29 0.76 0.74 0.55 0.71 – 0.65 0.6 0.67 0.68 0.45 0.27 0.18 0.48 0.41 0.3 0.69 0.8 0.63 0.54 0.42 0.43
Nuclear Periphery 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.44 – 0.47 0 0 0.09 0 – 0.09 0.12 0.11 0.1 0.05 0 0 0 0 0 0.22 0.08 0.21 0.18 0.17 0.12
Peroxisomes 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.09
Vac/Vac Membrane 0.17 – 0.29 0.19 0.22 0.31 0.23 – 0.2 0.27 0.16 0.17 0.41 0.5 0.52 0.4 0.47 0.46 0 0 0.06 0.09 0.11 0.1
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 0 0 0 – 0 1 0 2 0 0 0 1 0 0 0 0 0 0 0 0
Bud 0 – 0 0 0 2 1 – 3 3 3 3 0 1 0 1 0 0 1 1 0 21 46 35
Bud Neck 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 9
Bud Site 0 – 0 0 0 0 0 – 4 4 3 3 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2 – 0 11 21 13 33 – 13 21 13 17 30 104 175 17 17 27 0 1 0 10 36 43
Endoplasmic Reticulum 0 – 0 0 0 0 0 – 0 0 0 0 0 1 0 5 5 8 0 0 0 1 4 5
Endosome 0 – 0 5 1 2 3 – 4 6 3 4 7 4 18 3 1 1 1 0 2 11 35 26
Golgi 0 – 0 0 5 0 4 – 0 1 0 0 10 18 17 6 10 19 0 0 0 0 1 3
Mitochondria 0 – 0 0 2 18 1 – 24 13 19 21 1 0 0 1 1 1 0 0 0 1 1 0
Nucleus 18 – 5 156 288 119 273 – 183 205 155 244 133 113 97 72 54 44 50 55 30 188 303 244
Nuclear Periphery 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1 10 6
Nucleolus 16 – 8 0 7 19 9 – 24 42 26 37 15 6 4 1 2 6 16 5 10 64 126 70
Peroxisomes 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 – 0 0 1 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 1 14 59 49
Vac/Vac Membrane 6 – 5 38 84 66 89 – 56 93 36 61 119 214 289 60 63 68 0 2 2 30 78 58
Unique Cell Count 36 17 204 389 216 387 282 344 231 359 293 426 552 151 133 149 73 70 49 352 724 568
Labelled Cell Count 42 18 210 409 239 413 311 389 258 392 315 461 600 167 153 174 73 70 49 352 724 568


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.4 – 8.9 11.8 12.4 8.0 11.3 – 9.5 10.2 9.5 9.9 14.8 14.7 14.8 18.2 19.8 19.5 12.2 12.4 12.8
Std Deviation (1e-4) 2.5 – 1.3 2.1 1.9 2.0 2.2 – 1.9 1.9 1.8 2.0 2.3 2.2 2.2 4.4 4.7 5.7 1.7 1.9 2.0
Intensity Change (Log2) – – – 0.41 0.49 -0.15 0.35 – 0.1 0.2 0.11 0.16 0.74 0.73 0.74 1.04 1.16 1.14 0.46 0.48 0.53


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 – 0 0 0 0 0 0 2.8 0 0 0
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleus 4.2 4.0 2.0 3.6 – 2.9 2.5 3.1 3.3 1.3 0 0 1.4 0.9 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0.1 0 – 0 0 0 0 0.9 1.7 1.9 0.8 1.4 1.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.4128 8.3845 7.9062 7.1942 7.4512 7.5831 7.8478 10.7195 8.9789 9.2889 8.6335 9.4461 9.2871 11.9752 10.0649 10.5739 9.8481 10.4116
Actin 0.0143 0 0.0001 0 0 0.0005 0.0222 0 0.0013 0 0.0001 0 0.0108 0 0.0046 0.0077 0 0.0025
Bud 0.0002 0.0002 0.0007 0 0 0.0001 0.0002 0 0.0004 0.0001 0.0001 0.0001 0.0002 0 0.0002 0.0002 0 0.0001
Bud Neck 0.0007 0.0002 0.0002 0.0001 0.0005 0.0021 0.0007 0.0001 0.0004 0.0002 0.0009 0.0024 0.0002 0.0001 0.0006 0.0003 0.0007 0.0016
Bud Periphery 0.0001 0 0.0001 0 0 0.0001 0.0003 0 0.0009 0 0.0001 0.0001 0.0002 0 0.0006 0.0007 0 0.0001
Bud Site 0.0003 0.0001 0.0006 0 0.0001 0.0001 0.001 0.0001 0.0055 0.0001 0.0003 0.0001 0.0004 0.0001 0.0004 0.0004 0.0001 0.0001
Cell Periphery 0 0 0 0 0 0 0.0001 0 0.0004 0 0 0 0.0001 0 0.0002 0.0002 0 0
Cytoplasm 0.0037 0.0024 0.0043 0.0003 0.0166 0.0017 0.0044 0.0115 0.0002 0.0004 0.0005 0.0019 0.0032 0.0058 0.0021 0.0004 0.0005 0.0003
Cytoplasmic Foci 0.0045 0.0004 0.0026 0 0.0001 0.0004 0.0055 0 0 0 0 0 0.024 0 0.0071 0.004 0.0013 0.0003
Eisosomes 0.0001 0 0 0 0 0 0.0004 0 0.0001 0 0 0 0.0001 0 0.0001 0.0002 0 0
Endoplasmic Reticulum 0.0011 0 0 0 0.0004 0.0004 0.0018 0 0.0002 0 0.0001 0.0001 0.0005 0 0.0004 0.0008 0 0.0002
Endosome 0.0047 0 0 0 0.0004 0.0008 0.0053 0 0.0002 0 0.0001 0 0.0049 0 0.0051 0.0038 0.0003 0.0018
Golgi 0.0008 0 0 0 0 0.0001 0.0022 0 0.0001 0 0 0 0.0015 0 0.0017 0.0022 0 0.0017
Lipid Particles 0.003 0 0 0 0 0.0001 0.0069 0 0.0001 0 0 0 0.0081 0 0.0017 0.0121 0.0003 0.0011
Mitochondria 0.0006 0.0001 0.0008 0.0001 0.0002 0.0002 0.0097 0.0001 0.0005 0.0001 0.0002 0 0.0046 0.0001 0.0071 0.0147 0.0001 0.0002
None 0.0018 0.0002 0.0018 0 0.0003 0.0004 0.0034 0 0.0001 0 0 0 0.0095 0 0.004 0.0003 0.0001 0
Nuclear Periphery 0.0064 0.0016 0.0017 0.0027 0.0065 0.005 0.0058 0.001 0.0009 0.0009 0.0026 0.0015 0.0041 0.0006 0.0012 0.0033 0.0014 0.0006
Nucleolus 0.0076 0.0057 0.0075 0.0021 0.0078 0.0186 0.0085 0.0046 0.003 0.003 0.0149 0.0084 0.0064 0.0062 0.0048 0.0053 0.0199 0.0092
Nucleus 0.8959 0.9745 0.9351 0.9943 0.9635 0.917 0.904 0.9822 0.9847 0.9949 0.9778 0.9841 0.8963 0.9859 0.9496 0.9111 0.9279 0.9794
Peroxisomes 0.0078 0 0 0 0 0 0.0085 0 0.0001 0 0 0 0.004 0 0.0048 0.0012 0 0.0002
Punctate Nuclear 0.0456 0.0145 0.0443 0.0002 0.0028 0.0519 0.0079 0.0002 0.0001 0.0003 0.002 0.0006 0.019 0.001 0.0016 0.0293 0.0471 0.0005
Vacuole 0.0006 0.0001 0.0001 0.0001 0.0006 0.0003 0.0006 0.0001 0.0007 0 0.0003 0.0007 0.0009 0 0.0016 0.0009 0.0001 0.0001
Vacuole Periphery 0.0002 0 0 0 0.0001 0.0002 0.0006 0 0.0001 0 0.0001 0.0001 0.0008 0 0.0004 0.001 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 108.6197 134.5932 112.7149 99.3244 79.4784 135.4308 123.9365 122.0288 134.2453 101.5444
Translational Efficiency 1.1392 1.0229 1.1689 1.7788 1.761 1.1488 1.1523 1.9236 1.3394 1.363

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Peptidylprolyl-cis/trans-isomerase (PPIase); specific for phosphorylated S/T residues N-terminal to proline; regulates phosphorylation of RNAPII large subunit (Rpo21p) C-terminal domain (CTD) at Ser7; associates with phospho-Ser5 form of RNAPII in vivo; present along entire coding length of genes; represses initiation of CUTs; required for efficient termination of mRNA transcription, trimethylation of histone H3; human ortholog PIN1 can complement yeast null and ts mutants
Localization
Cell Percentages nucleus (92%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-5

Ess1

Ess1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ess1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available