Standard name
Human Ortholog
Description Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA1 has a paralog, GEA2, that arose from the whole genome duplication
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.07 0.05 0 0 0 0 0 0 0 0 0 0 0.05 0 0.08 0.2 0.16 0 0 0 0 0 0.06
Bud 0 0.05 0 0 0 0.09 0.08 0.09 0.11 0.08 0.11 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.13 0.08 0.08 0.07 0.06 0.07 0.05 0.07 0 0.08 0.06 0.17 0.17 0.29 0 0.07 0.15 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.12 0.09 0 0 0 0 0 0 0 0 0 0.07 0.16 0.06 0 0 0 0 0 0 0 0 0
Endosome 0.16 0 0 0 0 0 0.06 0 0 0 0 0 0.07 0.06 0 0.25 0 0.19 0.16 0.15 0.21 0.18 0.16 0.14
Golgi 0 0.13 0 0 0 0 0 0 0 0 0 0 0.17 0.15 0.05 0.58 0.75 0.52 0.68 0.41 0.34 0.54 0.47 0.62
Mitochondria 0.86 0.71 0.96 0.93 0.84 0.9 0.86 0.89 0.92 0.95 0.91 0.93 0.74 0.56 0.73 0.11 0.2 0.13 0.07 0.26 0.28 0.15 0.22 0.11
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0.05 0 0.06 0.07 0 0 0 0 0 0 0 0 0 0 0.05 0 0.09 0 0 0
SpindlePole 0 0 0 0 0.05 0 0.09 0.1 0.05 0 0 0.05 0 0 0 0 0.07 0.08 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 10 7 3 3 7 1 1 2 2 2 0 0 8 6 8 8 12 1 6 4 0 7 16
Bud 4 8 4 8 9 28 46 50 33 43 30 46 2 3 4 4 1 1 0 1 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 3 0 2 4 19 3 16 5 9 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 1 3 2 0 4 1 2 0 1 1 2 1 3 0 0 0 0 0 0
Cytoplasm 1 20 10 14 18 18 41 28 21 23 23 27 9 26 51 2 3 11 0 5 0 2 2 2
Endoplasmic Reticulum 1 18 12 1 12 9 3 3 1 7 0 1 4 24 10 3 0 0 0 2 0 0 2 0
Endosome 24 2 0 2 10 9 35 13 0 1 0 0 4 9 2 25 1 14 27 24 29 10 28 36
Golgi 4 19 0 1 3 1 0 0 1 0 0 0 9 22 8 59 30 39 114 67 47 30 81 167
Mitochondria 128 107 128 167 228 280 472 519 283 533 255 421 40 84 127 11 8 10 11 43 39 8 38 29
Nucleus 0 1 0 1 0 0 1 2 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 3 0 0 0 0 0 2 0 0 2 1 1 0 0 1 0 0 0 0 0 0 0 0
Nucleolus 3 0 0 1 0 1 6 8 3 4 1 1 0 0 0 0 0 2 0 0 0 0 0 0
Peroxisomes 4 0 0 2 13 8 31 42 6 15 6 7 0 0 0 3 0 0 8 3 12 0 3 8
SpindlePole 2 0 1 6 14 9 47 61 14 23 9 21 0 0 1 4 3 6 0 1 0 0 0 0
Vac/Vac Membrane 9 11 2 4 6 3 9 8 2 5 4 6 0 5 3 5 2 3 2 7 3 1 3 2
Unique Cell Count 149 150 133 179 270 310 550 584 308 564 279 453 54 151 175 102 40 75 168 166 139 56 173 268
Labelled Cell Count 180 199 164 213 316 376 699 758 371 676 339 543 69 182 213 127 57 101 168 166 139 56 173 268


Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.1 6.0 4.4 4.5 4.6 4.3 4.3 4.1 3.9 3.7 3.8 3.3 6.6 6.5 5.6 8.6 10.2 9.7 6.1 6.6 6.9
Std Deviation (1e-4) 0.9 1.4 0.5 0.8 1.1 0.9 1.2 1.0 1.5 0.9 1.4 0.8 1.1 1.0 1.0 1.1 1.8 1.3 0.9 1.2 1.1
Intensity Change (Log2) – – – 0.03 0.05 -0.03 -0.05 -0.1 -0.19 -0.26 -0.22 -0.41 0.59 0.55 0.35 0.96 1.21 1.14 0.46 0.58 0.64


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 -0.8 0.8 0 2.6
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.1 -0.3 -0.7 0 -1.3 -0.3 -1.7 0.3 -0.6 1.9 2.5 4.7 -1.9 0 1.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 1.7 -1.1 -1.9 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 6.0 0 5.2
Golgi 0 0 0 0 0 0 0 0 0 0 4.6 0 10.1 11.0 9.2
Mitochondria 0 0 0 0 0 0 0 0 0 -4.5 -7.9 -5.5 -13.2 -10.3 -12.2
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.2546 2.8569 2.3446 2.4565 2.2942 2.7284 2.2039 3.5756 3.504 3.1369 3.1312 3.5733 1.0327 1.2853 1.2184 0.9425 1.2257 0.6189
Actin 0.1057 0.0428 0.0494 0.0018 0.0782 0.0685 0.0686 0.0318 0.0258 0.0714 0.0179 0.0107 0.0708 0.0081 0.0139 0.0006 0.0087 0.0084
Bud 0.0008 0.0009 0.0006 0.0012 0.0008 0.0003 0.0011 0.0003 0.0008 0.001 0.0005 0.0002 0.0007 0.0009 0.0005 0 0.0001 0.0002
Bud Neck 0.002 0.0013 0.001 0.0027 0.0012 0.0025 0.0007 0.0005 0.0003 0.0014 0.0006 0.0006 0.0009 0.0008 0.0003 0.0004 0.0005 0.0005
Bud Periphery 0.0011 0.0011 0.0004 0.0014 0.0007 0.0004 0.0012 0.0001 0.0008 0.0009 0.0004 0.0002 0.0009 0.0011 0.0005 0 0.0001 0.0002
Bud Site 0.0041 0.0153 0.0041 0.0157 0.0038 0.0013 0.0064 0.008 0.0006 0.0006 0.0017 0.0003 0.0022 0.0047 0.0025 0.0001 0.0004 0.0002
Cell Periphery 0.0008 0.001 0.0001 0.0004 0.0001 0.0002 0.0002 0.0001 0.0001 0.0002 0.0001 0.0001 0.0004 0.0002 0.0005 0 0 0.0001
Cytoplasm 0.0102 0.0263 0.0056 0.0057 0.0015 0.0091 0.0105 0.0213 0.0043 0.0061 0.009 0.006 0.0337 0.0048 0.0213 0.0373 0.0035 0.0054
Cytoplasmic Foci 0.1176 0.1508 0.1268 0.1527 0.1193 0.1245 0.1624 0.1791 0.1089 0.0927 0.1312 0.1072 0.0884 0.2188 0.156 0.0656 0.1804 0.1048
Eisosomes 0.0007 0.0005 0.0001 0.0001 0.0001 0.0004 0.0003 0 0 0.0002 0.0001 0 0.001 0.0002 0.0003 0 0 0.0001
Endoplasmic Reticulum 0.0028 0.006 0.0018 0.0009 0.0005 0.0121 0.0064 0.0027 0.0017 0.0008 0.0006 0.0022 0.0049 0.0005 0.018 0.0019 0.0004 0.0026
Endosome 0.1731 0.1918 0.2276 0.2522 0.2145 0.1625 0.24 0.272 0.2801 0.3049 0.2668 0.1821 0.175 0.1341 0.2187 0.2323 0.3339 0.2421
Golgi 0.3923 0.3946 0.4898 0.4738 0.523 0.4887 0.3731 0.4488 0.5447 0.4666 0.5323 0.6308 0.2747 0.4367 0.3603 0.5796 0.3005 0.4622
Lipid Particles 0.0379 0.0134 0.0167 0.0076 0.0172 0.046 0.0376 0.0064 0.0089 0.0085 0.0046 0.0183 0.1007 0.0211 0.027 0.0051 0.0214 0.052
Mitochondria 0.0247 0.0931 0.0338 0.0067 0.0155 0.0432 0.0163 0.0036 0.011 0.0256 0.0181 0.0075 0.0386 0.1135 0.0403 0.0046 0.0289 0.0534
None 0.0613 0.0256 0.0008 0.0082 0.0001 0.0011 0.0072 0.0004 0.0002 0.0065 0.0002 0.0019 0.1365 0.0023 0.0438 0.0671 0.0003 0.003
Nuclear Periphery 0.0017 0.001 0.0004 0.0002 0.0001 0.0014 0.0104 0.0004 0.0003 0.0002 0.0002 0.0003 0.0142 0.0001 0.0049 0.0004 0.0001 0.0006
Nucleolus 0.0018 0.0003 0 0.0013 0 0.0001 0.0004 0 0 0.0007 0 0 0.0039 0.0003 0.0003 0.0001 0.0001 0.0002
Nucleus 0.0019 0.0006 0.0001 0.0006 0 0.0002 0.0027 0.0001 0.0001 0.0005 0.0001 0.0001 0.0136 0.0002 0.0015 0.0003 0.0001 0.0002
Peroxisomes 0.0281 0.0276 0.0375 0.0056 0.0216 0.0329 0.0428 0.0217 0.0081 0.0074 0.012 0.0295 0.0158 0.0489 0.0654 0.0018 0.1162 0.0532
Punctate Nuclear 0.0179 0.0006 0.0004 0.002 0.0003 0.0009 0.0049 0.0002 0.0002 0.0003 0.0003 0.0002 0.0136 0.0003 0.0008 0.0007 0.0004 0.0005
Vacuole 0.0106 0.0038 0.0016 0.0542 0.0006 0.0016 0.0029 0.0014 0.0015 0.0017 0.0018 0.0009 0.0058 0.0016 0.0176 0.0009 0.0025 0.0076
Vacuole Periphery 0.0028 0.0016 0.0014 0.0048 0.0008 0.002 0.0036 0.0012 0.0017 0.0021 0.0016 0.0011 0.0035 0.0008 0.0055 0.0012 0.0013 0.0027

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 25.7122 21.235 15.7573 19.1133 21.4913 16.1753 27.8411 24.1255 18.1627 27.6034
Translational Efficiency 0.6673 0.8051 0.7427 0.5499 0.6395 0.726 0.5247 0.5224 0.5813 0.5005

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA1 has a paralog, GEA2, that arose from the whole genome duplication
Localization
Cell Percentages Golgi (19%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Gea1

Gea1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gea1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available