Standard name
Human Ortholog
Description Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP85 aka NUP75

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.08 0 0 0 0 0 0 0 0 0.1 0.1 0.09 0.07 0.08 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0.05 0.13 0.08 0.08 0 0 0 0 0.05 0.07
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0.15 0.21 0.2 0.2 0.4 0.36 0.47 0.46 0.49 0.5 0.53 0 0 0 0.13 0.1 0.09 0 0 0 0.1 0.14 0.21
Nucleus 0 0.06 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0.06 0.1 0.05 0 0 0 0 0 0
Nuclear Periphery 0.74 0.76 0.68 0.74 0.64 0.54 0.59 0.42 0.45 0.58 0.53 0.57 0.76 0.76 0.72 0.53 0.51 0.56 0.88 0.77 0.87 0.71 0.7 0.57
Nucleolus 0.12 0.08 0.2 0.23 0.19 0.31 0.27 0.3 0.3 0.35 0.37 0.33 0.09 0.09 0.08 0.18 0.15 0.18 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Vac/Vac Membrane 0.35 0.27 0.19 0.06 0.17 0.07 0.07 0.05 0.06 0.05 0 0 0.22 0.24 0.26 0.28 0.27 0.31 0 0.06 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 1 1 3 2 4
Bud 0 0 0 0 0 1 2 2 2 2 4 2 0 0 0 0 2 0 2 6 3 0 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 1 4 7 9 5 2 2 0 0 1 1 0 0
Cell Periphery 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 1 2 0 0 0 0 0 0 0
Cytoplasm 7 6 7 8 8 7 7 11 11 4 4 3 14 14 16 20 24 13 0 1 0 0 1 0
Endoplasmic Reticulum 0 0 1 1 0 2 0 1 0 0 1 0 1 1 1 2 12 3 0 0 0 0 0 1
Endosome 3 1 11 3 16 10 12 11 10 5 5 7 6 6 9 39 23 15 3 9 2 5 15 18
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 3 0 2 1 1 5 5
Mitochondria 24 49 55 21 45 115 125 224 224 167 134 192 0 0 3 37 29 18 6 12 11 14 47 56
Nucleus 10 19 5 3 6 3 7 19 19 15 12 12 8 8 7 19 27 10 3 5 1 0 0 0
Nuclear Periphery 221 244 180 77 144 155 204 201 215 199 143 205 112 109 125 157 145 111 289 278 252 100 238 152
Nucleolus 36 27 53 24 42 87 94 144 144 121 101 118 13 13 13 54 42 36 4 15 2 2 3 2
Peroxisomes 0 0 0 0 2 0 3 5 5 2 1 0 0 0 0 1 1 2 0 0 1 0 5 9
SpindlePole 1 0 2 0 3 3 7 8 8 4 1 2 2 2 5 12 13 7 1 2 3 3 4 3
Vac/Vac Membrane 103 87 51 6 38 20 24 24 27 16 8 12 33 35 45 81 77 61 10 21 4 4 10 9
Unique Cell Count 298 323 264 104 225 285 346 477 482 342 270 360 147 143 173 294 284 199 328 360 292 142 341 269
Labelled Cell Count 405 433 368 143 304 404 489 657 674 540 416 555 189 188 227 424 398 279 328 360 292 142 341 269


Nuclear Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.8 11.5 11.3 10.0 9.2 7.9 7.6 7.7 7.7 6.8 7.1 7.0 10.8 10.8 10.9 7.1 7.3 9.7 11.7 12.4 12.8
Std Deviation (1e-4) 2.7 2.9 3.3 2.6 2.2 2.5 2.2 2.6 2.6 1.9 2.0 2.0 3.0 3.0 3.5 1.3 1.9 2.4 2.6 2.5 3.1
Intensity Change (Log2) -0.17 -0.3 -0.52 -0.57 -0.55 -0.55 -0.72 -0.67 -0.68 -0.07 -0.07 -0.06 -0.68 -0.63 -0.22 0.04 0.14 0.18


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.6 -0.1 -0.5 -0.3 -0.3 0 -1.0 0 3.0 3.1 3.0 2.3 2.9 2.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 3.0 0
Endosome 0 1.4 -0.4 -0.4 -1.4 -1.7 -2.1 -1.6 -1.6 0 0 0.5 3.8 1.9 1.6
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -0.1 -0.2 4.9 4.1 7.0 6.9 7.1 7.0 8.2 -5.9 -5.9 -5.8 -2.6 -3.4 -3.4
Nucleus 0 0.6 0 0.1 1.5 1.5 1.7 1.7 1.1 0 0 0 2.7 3.8 1.9
Nuclear Periphery 1.1 -1.0 -3.3 -2.3 -6.8 -6.2 -2.5 -3.6 -2.9 1.7 1.7 0.9 -3.6 -4.1 -2.7
Nucleolus 0.6 -0.4 2.8 2.0 3.0 2.9 4.1 4.4 3.5 -3.0 -2.9 -3.6 -0.5 -1.6 -0.5
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 2.5 2.7 0
Vacuole -3.2 -0.7 -4.3 -4.6 -6.2 -5.9 -5.7 -6.0 -6.5 0.8 1.2 1.7 2.3 2.2 2.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.4948 7.1391 6.2739 6.3773 5.6201 6.1661 8.2773 8.6007 7.5013 6.3457 6.6794 7.4757 10.2296 8.9191 8.9538 8.8107 9.407 8.6231
Actin 0.0026 0.0003 0.0007 0.0015 0.0013 0.0001 0.0036 0.0004 0.0008 0.001 0.0073 0.0005 0.0001 0.0002 0.0003 0.0008 0.0002 0.0001
Bud 0.0001 0 0 0.0001 0 0 0.0002 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0.0001 0 0 0.0001 0.0001 0 0.0002 0 0 0 0 0 0 0 0 0 0 0
Bud Periphery 0.0004 0.0001 0.0001 0.0003 0.0002 0 0.0007 0.0002 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001 0
Bud Site 0.0001 0 0.0001 0.0002 0 0 0.0013 0.0001 0 0 0.0001 0 0 0 0 0 0 0
Cell Periphery 0.0001 0 0 0.0001 0.0001 0 0.0003 0.0001 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.0011 0 0.0003 0.0005 0.0001 0 0.0011 0 0.0001 0 0 0 0 0 0 0 0 0
Cytoplasmic Foci 0.0038 0.0011 0.0005 0.0013 0.0002 0.0001 0.0014 0.0033 0.0002 0.0009 0.0025 0.0008 0.0001 0.0001 0.0001 0.0001 0 0
Eisosomes 0.0001 0 0 0.0001 0.0001 0 0.0003 0.0001 0 0 0.0001 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0019 0.0014 0.001 0.0019 0.0016 0.0003 0.0021 0.001 0.0016 0.0013 0.0007 0.0003 0.0003 0.0006 0.0007 0.0009 0.0004 0.0002
Endosome 0.0055 0.0006 0.0009 0.0032 0.0022 0.0009 0.0037 0.0015 0.0014 0.0005 0.0014 0.0003 0.0002 0.0003 0.0004 0.0005 0.0004 0.0002
Golgi 0.0013 0.001 0.0004 0.0009 0.0013 0.0003 0.0016 0.0057 0.0007 0.0005 0.0043 0.0003 0.0001 0.0002 0.0002 0.0004 0.0004 0.0002
Lipid Particles 0.0194 0.0453 0.0093 0.0058 0.0177 0.0067 0.0032 0.0262 0.0085 0.0042 0.0122 0.0037 0.0024 0.0032 0.0032 0.0021 0.0047 0.002
Mitochondria 0.0093 0.0018 0.0019 0.0021 0.0071 0.0011 0.0147 0.006 0.0025 0.0035 0.0083 0.0033 0.0011 0.0006 0.0008 0.0012 0.0034 0.0008
None 0.0038 0.0002 0.0004 0.001 0.0006 0.0001 0.0015 0.0003 0.0005 0.0002 0.0001 0.0001 0.0002 0.0001 0.0002 0.0002 0.0002 0.0001
Nuclear Periphery 0.9022 0.8996 0.9236 0.908 0.9024 0.9653 0.9039 0.8861 0.9258 0.8976 0.9001 0.9668 0.963 0.9497 0.9513 0.9575 0.9588 0.9812
Nucleolus 0.001 0.001 0.0036 0.0041 0.0028 0.0012 0.0008 0.0061 0.0017 0.0004 0.0003 0.0002 0.0003 0.0028 0.0006 0.0005 0.0006 0.0003
Nucleus 0.0042 0.0128 0.0088 0.0276 0.0233 0.0028 0.0037 0.0024 0.0085 0.0039 0.0014 0.001 0.0017 0.0057 0.0038 0.0023 0.0016 0.0013
Peroxisomes 0.0011 0.0005 0.0001 0.0002 0.0002 0 0.0012 0.0009 0.0001 0.0521 0.0347 0.007 0 0 0.0001 0.0001 0 0
Punctate Nuclear 0.0048 0.0018 0.0125 0.0095 0.0018 0.0023 0.0177 0.0021 0.0014 0.0007 0.0007 0.0006 0.0005 0.0031 0.002 0.001 0.001 0.0008
Vacuole 0.0011 0.0005 0.0006 0.0011 0.0009 0.0003 0.001 0.0009 0.0007 0.0003 0.0003 0.0001 0.0003 0.0004 0.0003 0.0003 0.0003 0.0001
Vacuole Periphery 0.0359 0.0321 0.035 0.0303 0.036 0.0184 0.0358 0.0566 0.0452 0.0327 0.0252 0.0147 0.0295 0.033 0.0358 0.0318 0.0278 0.0126

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 21.9694 21.6252 26.8539 32.3119 26.5639 24.1776 28.3356 30.8705 30.1059 31.1292
Translational Efficiency 1.6071 1.4054 1.0882 0.9036 1.0276 1.1344 1.0607 0.8299 0.8547 0.8968

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP85 aka NUP75
Localization
Cell Percentages nuclear periphery (93%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Nup85

Nup85


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nup85-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available