Standard name | |
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Human Ortholog | |
Description | Third subunit of DNA polymerase delta; involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; forms a complex with Rev3p, Rev7p and Pol31p; interacts with Hys2p, PCNA (Pol30p), and Pol1p |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 1 | 1 | 1 | 0 | 1 | 3 |
Bud | 0 | 1 | 0 | 0 | 0 | 0 | 5 | 3 | 3 | 1 | 3 | 3 | 0 | 0 | 0 | – | – | – | 1 | 0 | 0 | 1 | 1 | 4 |
Bud Neck | 0 | 0 | 5 | 2 | 6 | 5 | 6 | 8 | 5 | 4 | 2 | 6 | 1 | 0 | 0 | – | – | – | 0 | 1 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | – | – | – | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 0 | 2 | 0 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 5 | 0 | 0 | – | – | – | 2 | 1 | 1 | 0 | 0 | 0 |
Cytoplasm | 252 | 182 | 166 | 198 | 268 | 283 | 348 | 275 | 194 | 270 | 173 | 163 | 153 | 139 | 158 | – | – | – | 222 | 145 | 126 | 59 | 130 | 158 |
Endoplasmic Reticulum | 1 | 1 | 1 | 0 | 2 | 0 | 0 | 0 | 5 | 1 | 1 | 2 | 1 | 0 | 0 | – | – | – | 0 | 1 | 1 | 0 | 0 | 0 |
Endosome | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 2 | 2 | 1 | 0 | 0 | 1 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 1 | 1 | 0 | 0 | 0 | 0 |
Mitochondria | 14 | 3 | 0 | 0 | 5 | 23 | 71 | 59 | 83 | 69 | 125 | 72 | 0 | 0 | 0 | – | – | – | 20 | 23 | 15 | 2 | 3 | 2 |
Nucleus | 0 | 1 | 0 | 0 | 1 | 2 | 2 | 3 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 0 | 1 | 1 | 1 | 7 | 4 | 2 | 1 | 4 | 3 | 0 | 0 | 0 | – | – | – | 7 | 2 | 1 | 1 | 2 | 1 |
Vac/Vac Membrane | 1 | 0 | 0 | 0 | 0 | 4 | 6 | 1 | 2 | 1 | 5 | 2 | 0 | 0 | 1 | – | – | – | 5 | 11 | 1 | 1 | 1 | 3 |
Unique Cell Count | 260 | 185 | 167 | 201 | 272 | 293 | 390 | 317 | 258 | 302 | 241 | 227 | 154 | 139 | 158 | 274 | 197 | 159 | 69 | 147 | 186 | |||
Labelled Cell Count | 271 | 189 | 173 | 204 | 284 | 322 | 446 | 353 | 298 | 348 | 314 | 253 | 160 | 139 | 159 | 274 | 197 | 159 | 69 | 147 | 186 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.2 | 3.9 | 4.5 | 4.4 | 4.3 | 3.6 | 3.3 | 3.8 | 2.9 | 3.2 | 2.9 | 3.3 | 4.5 | 4.8 | 4.8 | – | 4.9 | – | 4.3 | 4.5 | 5.3 |
Std Deviation (1e-4) | 0.7 | 0.9 | 1.3 | 1.6 | 1.3 | 1.6 | 1.5 | 1.5 | 0.7 | 0.9 | 1.0 | 0.9 | 1.1 | 1.1 | 1.4 | – | 0.8 | – | 1.9 | 1.5 | 2.1 |
Intensity Change (Log2) | – | – | – | -0.05 | -0.08 | -0.34 | -0.44 | -0.25 | -0.63 | -0.5 | -0.67 | -0.44 | 0.01 | 0.07 | 0.09 | – | 0.11 | – | -0.06 | -0.02 | 0.22 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.3445 | 0.0224 | 0.1585 | 0.5017 | 0.2693 | 0.2488 | -0.6219 | -0.5793 | -0.3439 | -0.7691 | -0.4313 | -0.6233 | -1.1584 | -0.2463 | -0.8432 | -0.687 | -1.4413 | -1.1215 |
Actin | 0.0242 | 0.0001 | 0.0202 | 0.0779 | 0.0024 | 0.045 | 0.0255 | 0.0002 | 0.016 | 0.0021 | 0.048 | 0.0049 | 0.0447 | 0.0096 | 0.0113 | 0.019 | 0.031 | 0.0014 |
Bud | 0.0006 | 0.0024 | 0.001 | 0.0022 | 0.0002 | 0.0003 | 0.001 | 0.0041 | 0.0008 | 0.0001 | 0.0026 | 0.0001 | 0.0006 | 0.0011 | 0.004 | 0.0004 | 0.0004 | 0.0002 |
Bud Neck | 0.0005 | 0.0001 | 0.0002 | 0.0037 | 0.0002 | 0.0038 | 0.0195 | 0.0001 | 0.0137 | 0.001 | 0.0003 | 0.0006 | 0.0009 | 0.0017 | 0.0023 | 0.0002 | 0.0004 | 0.0006 |
Bud Periphery | 0.0009 | 0.0023 | 0.0019 | 0.006 | 0.0003 | 0.0011 | 0.0038 | 0.0029 | 0.001 | 0.0002 | 0.0046 | 0.0002 | 0.0007 | 0.0031 | 0.0038 | 0.0008 | 0.0006 | 0.0004 |
Bud Site | 0.0032 | 0.0078 | 0.0056 | 0.0097 | 0.0004 | 0.0004 | 0.0045 | 0.0036 | 0.0171 | 0.0003 | 0.0008 | 0.0002 | 0.0048 | 0.0108 | 0.0047 | 0.0007 | 0.0006 | 0.0001 |
Cell Periphery | 0.0002 | 0.0001 | 0.0001 | 0.0009 | 0.0001 | 0.0003 | 0.0005 | 0.0001 | 0.0004 | 0.0002 | 0.0002 | 0 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0 |
Cytoplasm | 0.0478 | 0.0653 | 0.0617 | 0.0522 | 0.0275 | 0.1076 | 0.0361 | 0.0303 | 0.0473 | 0.0699 | 0.0664 | 0.1043 | 0.0949 | 0.1362 | 0.1413 | 0.1064 | 0.2311 | 0.217 |
Cytoplasmic Foci | 0.0487 | 0.0043 | 0.005 | 0.0279 | 0.0341 | 0.0195 | 0.0418 | 0.0081 | 0.0171 | 0.0117 | 0.0096 | 0.0086 | 0.0173 | 0.0115 | 0.0166 | 0.0234 | 0.0072 | 0.0095 |
Eisosomes | 0.0005 | 0.0001 | 0.0002 | 0.0009 | 0.0001 | 0.0006 | 0.0009 | 0.0001 | 0.0006 | 0.0009 | 0.001 | 0.0001 | 0.0004 | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0021 | 0.0009 | 0.0009 | 0.0014 | 0.0004 | 0.0016 | 0.0036 | 0.0003 | 0.0027 | 0.0008 | 0.0034 | 0.001 | 0.0019 | 0.002 | 0.002 | 0.0017 | 0.0013 | 0.002 |
Endosome | 0.0448 | 0.0005 | 0.0013 | 0.0173 | 0.0493 | 0.0046 | 0.0248 | 0.0008 | 0.0121 | 0.0023 | 0.0142 | 0.0049 | 0.0291 | 0.0048 | 0.0132 | 0.0079 | 0.0038 | 0.0098 |
Golgi | 0.0213 | 0 | 0.0016 | 0.0103 | 0.0322 | 0.0047 | 0.0093 | 0.0001 | 0.0028 | 0.0001 | 0.0137 | 0.0027 | 0.0189 | 0.0015 | 0.0094 | 0.0036 | 0.0109 | 0.0018 |
Lipid Particles | 0.0219 | 0.0003 | 0.0017 | 0.0498 | 0.0536 | 0.0046 | 0.017 | 0.0002 | 0.0045 | 0.0005 | 0.0366 | 0.0058 | 0.0165 | 0.0009 | 0.0031 | 0.0125 | 0.0014 | 0.0071 |
Mitochondria | 0.0305 | 0.0002 | 0.0036 | 0.0281 | 0.0698 | 0.019 | 0.0077 | 0.0004 | 0.0034 | 0.0005 | 0.0438 | 0.0103 | 0.0055 | 0.0054 | 0.0049 | 0.0074 | 0.015 | 0.0038 |
None | 0.6918 | 0.9026 | 0.8843 | 0.6321 | 0.6971 | 0.7526 | 0.7326 | 0.9414 | 0.8227 | 0.8944 | 0.7145 | 0.8018 | 0.7235 | 0.7947 | 0.7349 | 0.7623 | 0.659 | 0.6879 |
Nuclear Periphery | 0.0134 | 0.0006 | 0.001 | 0.002 | 0.0006 | 0.002 | 0.0074 | 0.0004 | 0.0122 | 0.0012 | 0.0088 | 0.0025 | 0.0015 | 0.0014 | 0.0053 | 0.0037 | 0.0015 | 0.0024 |
Nucleolus | 0.0007 | 0.0002 | 0.0003 | 0.0007 | 0.0062 | 0.0003 | 0.0042 | 0.0004 | 0.0038 | 0.0017 | 0.0009 | 0.0006 | 0.0004 | 0.0005 | 0.0022 | 0.0009 | 0.0003 | 0.0006 |
Nucleus | 0.0143 | 0.0044 | 0.0025 | 0.0031 | 0.0016 | 0.0075 | 0.0121 | 0.0016 | 0.0082 | 0.0047 | 0.01 | 0.0353 | 0.0084 | 0.007 | 0.02 | 0.0249 | 0.0262 | 0.0283 |
Peroxisomes | 0.0135 | 0.0001 | 0.0045 | 0.0678 | 0.008 | 0.0116 | 0.0189 | 0.0002 | 0.0032 | 0.0003 | 0.0096 | 0.0033 | 0.0257 | 0.0026 | 0.0045 | 0.0131 | 0.003 | 0.0014 |
Punctate Nuclear | 0.0145 | 0.0075 | 0.002 | 0.0038 | 0.014 | 0.0119 | 0.0198 | 0.004 | 0.0072 | 0.0064 | 0.0071 | 0.0122 | 0.0018 | 0.0037 | 0.013 | 0.0093 | 0.0054 | 0.0244 |
Vacuole | 0.0031 | 0.0003 | 0.0005 | 0.0016 | 0.0011 | 0.0006 | 0.0075 | 0.0006 | 0.0021 | 0.0006 | 0.0016 | 0.0004 | 0.0019 | 0.0009 | 0.0024 | 0.0009 | 0.0006 | 0.0007 |
Vacuole Periphery | 0.0017 | 0 | 0.0001 | 0.0007 | 0.0008 | 0.0003 | 0.0014 | 0.0001 | 0.0008 | 0.0001 | 0.0022 | 0.0002 | 0.0006 | 0.0001 | 0.0008 | 0.0005 | 0.0002 | 0.0004 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 30.9121 | 9.3673 | 11.1215 | 17.2279 | 24.4549 | 38.7496 | 10.4184 | 9.9083 | 13.5141 | 22.9525 |
Translational Efficiency | 1.2114 | 1.3663 | 1.0857 | 0.9066 | 0.9766 | 0.9206 | 1.1985 | 1.3244 | 1.1156 | 1.2348 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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