Standard name
Human Ortholog
Description Third subunit of DNA polymerase delta; involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; forms a complex with Rev3p, Rev7p and Pol31p; interacts with Hys2p, PCNA (Pol30p), and Pol1p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.98 0.99 0.99 0.99 0.97 0.89 0.87 0.75 0.89 0.72 0.72 0.99 1.0 1.0 0.81 0.74 0.79 0.86 0.89 0.85
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0 0 0 0 0.08 0.18 0.19 0.32 0.23 0.52 0.32 0 0 0 0.07 0.12 0.1 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 1 0 0 0 1 0 0 0 0 0 0 1 1 1 0 1 3
Bud 0 1 0 0 0 0 5 3 3 1 3 3 0 0 0 1 0 0 1 1 4
Bud Neck 0 0 5 2 6 5 6 8 5 4 2 6 1 0 0 0 1 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0
Cell Periphery 1 0 0 2 0 4 1 0 0 0 0 0 5 0 0 2 1 1 0 0 0
Cytoplasm 252 182 166 198 268 283 348 275 194 270 173 163 153 139 158 222 145 126 59 130 158
Endoplasmic Reticulum 1 1 1 0 2 0 0 0 5 1 1 2 1 0 0 0 1 1 0 0 0
Endosome 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 2 1 0 0 1
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0
Mitochondria 14 3 0 0 5 23 71 59 83 69 125 72 0 0 0 20 23 15 2 3 2
Nucleus 0 1 0 0 1 2 2 3 3 0 0 1 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 1 1 1 7 4 2 1 4 3 0 0 0 7 2 1 1 2 1
Vac/Vac Membrane 1 0 0 0 0 4 6 1 2 1 5 2 0 0 1 5 11 1 1 1 3
Unique Cell Count 260 185 167 201 272 293 390 317 258 302 241 227 154 139 158 274 197 159 69 147 186
Labelled Cell Count 271 189 173 204 284 322 446 353 298 348 314 253 160 139 159 274 197 159 69 147 186


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.2 3.9 4.5 4.4 4.3 3.6 3.3 3.8 2.9 3.2 2.9 3.3 4.5 4.8 4.8 4.9 4.3 4.5 5.3
Std Deviation (1e-4) 0.7 0.9 1.3 1.6 1.3 1.6 1.5 1.5 0.7 0.9 1.0 0.9 1.1 1.1 1.4 0.8 1.9 1.5 2.1
Intensity Change (Log2) -0.05 -0.08 -0.34 -0.44 -0.25 -0.63 -0.5 -0.67 -0.44 0.01 0.07 0.09 0.11 -0.06 -0.02 0.22


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.3445 0.0224 0.1585 0.5017 0.2693 0.2488 -0.6219 -0.5793 -0.3439 -0.7691 -0.4313 -0.6233 -1.1584 -0.2463 -0.8432 -0.687 -1.4413 -1.1215
Actin 0.0242 0.0001 0.0202 0.0779 0.0024 0.045 0.0255 0.0002 0.016 0.0021 0.048 0.0049 0.0447 0.0096 0.0113 0.019 0.031 0.0014
Bud 0.0006 0.0024 0.001 0.0022 0.0002 0.0003 0.001 0.0041 0.0008 0.0001 0.0026 0.0001 0.0006 0.0011 0.004 0.0004 0.0004 0.0002
Bud Neck 0.0005 0.0001 0.0002 0.0037 0.0002 0.0038 0.0195 0.0001 0.0137 0.001 0.0003 0.0006 0.0009 0.0017 0.0023 0.0002 0.0004 0.0006
Bud Periphery 0.0009 0.0023 0.0019 0.006 0.0003 0.0011 0.0038 0.0029 0.001 0.0002 0.0046 0.0002 0.0007 0.0031 0.0038 0.0008 0.0006 0.0004
Bud Site 0.0032 0.0078 0.0056 0.0097 0.0004 0.0004 0.0045 0.0036 0.0171 0.0003 0.0008 0.0002 0.0048 0.0108 0.0047 0.0007 0.0006 0.0001
Cell Periphery 0.0002 0.0001 0.0001 0.0009 0.0001 0.0003 0.0005 0.0001 0.0004 0.0002 0.0002 0 0.0002 0.0002 0.0002 0.0001 0.0001 0
Cytoplasm 0.0478 0.0653 0.0617 0.0522 0.0275 0.1076 0.0361 0.0303 0.0473 0.0699 0.0664 0.1043 0.0949 0.1362 0.1413 0.1064 0.2311 0.217
Cytoplasmic Foci 0.0487 0.0043 0.005 0.0279 0.0341 0.0195 0.0418 0.0081 0.0171 0.0117 0.0096 0.0086 0.0173 0.0115 0.0166 0.0234 0.0072 0.0095
Eisosomes 0.0005 0.0001 0.0002 0.0009 0.0001 0.0006 0.0009 0.0001 0.0006 0.0009 0.001 0.0001 0.0004 0.0002 0.0002 0.0003 0.0001 0.0001
Endoplasmic Reticulum 0.0021 0.0009 0.0009 0.0014 0.0004 0.0016 0.0036 0.0003 0.0027 0.0008 0.0034 0.001 0.0019 0.002 0.002 0.0017 0.0013 0.002
Endosome 0.0448 0.0005 0.0013 0.0173 0.0493 0.0046 0.0248 0.0008 0.0121 0.0023 0.0142 0.0049 0.0291 0.0048 0.0132 0.0079 0.0038 0.0098
Golgi 0.0213 0 0.0016 0.0103 0.0322 0.0047 0.0093 0.0001 0.0028 0.0001 0.0137 0.0027 0.0189 0.0015 0.0094 0.0036 0.0109 0.0018
Lipid Particles 0.0219 0.0003 0.0017 0.0498 0.0536 0.0046 0.017 0.0002 0.0045 0.0005 0.0366 0.0058 0.0165 0.0009 0.0031 0.0125 0.0014 0.0071
Mitochondria 0.0305 0.0002 0.0036 0.0281 0.0698 0.019 0.0077 0.0004 0.0034 0.0005 0.0438 0.0103 0.0055 0.0054 0.0049 0.0074 0.015 0.0038
None 0.6918 0.9026 0.8843 0.6321 0.6971 0.7526 0.7326 0.9414 0.8227 0.8944 0.7145 0.8018 0.7235 0.7947 0.7349 0.7623 0.659 0.6879
Nuclear Periphery 0.0134 0.0006 0.001 0.002 0.0006 0.002 0.0074 0.0004 0.0122 0.0012 0.0088 0.0025 0.0015 0.0014 0.0053 0.0037 0.0015 0.0024
Nucleolus 0.0007 0.0002 0.0003 0.0007 0.0062 0.0003 0.0042 0.0004 0.0038 0.0017 0.0009 0.0006 0.0004 0.0005 0.0022 0.0009 0.0003 0.0006
Nucleus 0.0143 0.0044 0.0025 0.0031 0.0016 0.0075 0.0121 0.0016 0.0082 0.0047 0.01 0.0353 0.0084 0.007 0.02 0.0249 0.0262 0.0283
Peroxisomes 0.0135 0.0001 0.0045 0.0678 0.008 0.0116 0.0189 0.0002 0.0032 0.0003 0.0096 0.0033 0.0257 0.0026 0.0045 0.0131 0.003 0.0014
Punctate Nuclear 0.0145 0.0075 0.002 0.0038 0.014 0.0119 0.0198 0.004 0.0072 0.0064 0.0071 0.0122 0.0018 0.0037 0.013 0.0093 0.0054 0.0244
Vacuole 0.0031 0.0003 0.0005 0.0016 0.0011 0.0006 0.0075 0.0006 0.0021 0.0006 0.0016 0.0004 0.0019 0.0009 0.0024 0.0009 0.0006 0.0007
Vacuole Periphery 0.0017 0 0.0001 0.0007 0.0008 0.0003 0.0014 0.0001 0.0008 0.0001 0.0022 0.0002 0.0006 0.0001 0.0008 0.0005 0.0002 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 30.9121 9.3673 11.1215 17.2279 24.4549 38.7496 10.4184 9.9083 13.5141 22.9525
Translational Efficiency 1.2114 1.3663 1.0857 0.9066 0.9766 0.9206 1.1985 1.3244 1.1156 1.2348

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Third subunit of DNA polymerase delta; involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; forms a complex with Rev3p, Rev7p and Pol31p; interacts with Hys2p, PCNA (Pol30p), and Pol1p
Localization
Cell Percentages cytoplasm (10%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Pol32

Pol32


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pol32-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available