Standard name | |
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Human Ortholog | |
Description | Cytochrome c, isoform 1; also known as iso-1-cytochrome c; electron carrier of mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration; CYC1 has a paralog, CYC7, that arose from the whole genome duplication; human homolog CYC1 can complement yeast null mutant; mutations in human CYC1 cause insulin-responsive hyperglycemia |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 9 | 5 | 6 | 7 | 3 | 0 | – | 0 | 1 | 3 | 1 | 0 | 6 | 3 | 1 | – | – | – | 7 | 21 | 20 | 6 | 16 | 14 |
Bud | 2 | 2 | 7 | 7 | 3 | 6 | – | 8 | 11 | 14 | 22 | 12 | 1 | 0 | 3 | – | – | – | 6 | 13 | 3 | 6 | 12 | 13 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 5 | 5 | 2 | 1 | 2 | 7 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 1 | 4 | 3 | 8 | 0 | 0 | 0 | – | – | – | – | – | – | – | – | – |
Cell Periphery | 2 | 10 | 8 | 8 | 3 | 0 | – | 2 | 5 | 10 | 3 | 4 | 6 | 1 | 4 | – | – | – | 0 | 1 | 4 | 1 | 1 | 2 |
Cytoplasm | 178 | 135 | 67 | 67 | 71 | 45 | – | 42 | 38 | 37 | 31 | 33 | 113 | 141 | 149 | – | – | – | 129 | 83 | 27 | 15 | 31 | 27 |
Endoplasmic Reticulum | 16 | 10 | 6 | 9 | 9 | 3 | – | 1 | 4 | 5 | 1 | 7 | 12 | 13 | 7 | – | – | – | 6 | 15 | 28 | 4 | 11 | 7 |
Endosome | 3 | 25 | 18 | 18 | 75 | 15 | – | 22 | 10 | 11 | 4 | 4 | 7 | 16 | 21 | – | – | – | 37 | 50 | 42 | 21 | 45 | 29 |
Golgi | 0 | 7 | 5 | 3 | 7 | 1 | – | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | – | – | – | 3 | 19 | 21 | 13 | 22 | 11 |
Mitochondria | 10 | 13 | 152 | 152 | 106 | 165 | – | 255 | 293 | 503 | 272 | 417 | 6 | 4 | 8 | – | – | – | 24 | 57 | 71 | 32 | 50 | 64 |
Nucleus | 1 | 1 | 0 | 0 | 0 | 3 | – | 5 | 2 | 11 | 8 | 5 | 0 | 1 | 0 | – | – | – | 5 | 3 | 0 | 0 | 1 | 2 |
Nuclear Periphery | 0 | 2 | 0 | 0 | 1 | 0 | – | 4 | 7 | 10 | 3 | 11 | 0 | 2 | 0 | – | – | – | 3 | 3 | 1 | 1 | 1 | 2 |
Nucleolus | 0 | 1 | 1 | 1 | 1 | 1 | – | 6 | 9 | 8 | 5 | 9 | 0 | 1 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 2 | 2 | 3 | 1 | – | 1 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | – | – | – | 0 | 0 | 2 | 1 | 0 | 0 |
SpindlePole | 0 | 1 | 1 | 1 | 4 | 1 | – | 5 | 4 | 5 | 9 | 7 | 0 | 0 | 0 | – | – | – | 4 | 3 | 8 | 2 | 7 | 5 |
Vac/Vac Membrane | 12 | 76 | 24 | 20 | 74 | 37 | – | 68 | 53 | 64 | 25 | 38 | 22 | 17 | 38 | – | – | – | 17 | 35 | 5 | 16 | 15 | 25 |
Unique Cell Count | 219 | 252 | 238 | 240 | 277 | 227 | 331 | 347 | 560 | 308 | 450 | 165 | 189 | 213 | 258 | 325 | 250 | 130 | 226 | 221 | ||||
Labelled Cell Count | 233 | 288 | 297 | 295 | 360 | 278 | 420 | 439 | 686 | 387 | 556 | 174 | 201 | 232 | 258 | 325 | 250 | 130 | 226 | 221 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.9 | 9.7 | 7.3 | 7.3 | 7.4 | 5.4 | – | 5.3 | 4.6 | 4.8 | 5.0 | 4.8 | 8.8 | 8.8 | 7.8 | – | – | – | 9.0 | 8.5 | 8.1 |
Std Deviation (1e-4) | 1.9 | 2.1 | 2.5 | 2.5 | 2.5 | 2.0 | – | 2.1 | 1.5 | 1.5 | 1.6 | 1.4 | 2.2 | 2.4 | 2.0 | – | – | – | 2.6 | 2.3 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.0 | 0.03 | -0.44 | – | -0.45 | -0.66 | -0.59 | -0.53 | -0.59 | 0.27 | 0.28 | 0.1 | – | – | – | 0.31 | 0.23 | 0.16 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0.3 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud | 0 | 0 | -0.2 | – | -0.4 | 0 | 0 | 2.2 | 0 | 0 | 0 | 0 | – | – | – |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Cell Periphery | 0 | -1.8 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0.1 | 0 | -1.0 | – | – | – |
Cytoplasm | -0.1 | -0.6 | -2.1 | – | -4.6 | -5.3 | -8.3 | -5.5 | -7.4 | 8.0 | 9.5 | 8.9 | – | – | – |
Endoplasmic Reticulum | 0.8 | 0.5 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2.3 | 2.2 | 0.5 | – | – | – |
Endosome | 0 | 5.7 | -0.4 | – | -0.4 | 0 | 0 | -3.7 | 0 | -1.4 | 0.3 | 0.9 | – | – | – |
Golgi | 0 | 0.3 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Mitochondria | -0.1 | -5.8 | 2.0 | – | 3.4 | 0 | 0 | 6.8 | 0 | -12.2 | -13.2 | -13.3 | – | – | – |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Vacuole | -0.7 | 4.8 | 2.0 | – | 3.3 | 1.8 | 0.6 | -0.8 | -0.7 | 1.0 | -0.4 | 2.4 | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.572 | 6.5602 | 5.8206 | 5.449 | 4.4412 | 5.9994 | 4.7345 | 6.505 | 7.1294 | 6.6103 | 4.9566 | 7.1751 | 6.2097 | 8.9913 | 8.2635 | 8.3549 | 6.0155 | 7.8441 |
Actin | 0.0697 | 0.0429 | 0.042 | 0.0098 | 0.0773 | 0.0407 | 0.0561 | 0.0582 | 0.0621 | 0.0389 | 0.0111 | 0.0216 | 0.058 | 0.0748 | 0.0629 | 0.0227 | 0.1221 | 0.0392 |
Bud | 0.0024 | 0.0079 | 0.0073 | 0.0104 | 0.0112 | 0.0036 | 0.0051 | 0.0091 | 0.0077 | 0.0182 | 0.0016 | 0.0029 | 0.0034 | 0.0101 | 0.0049 | 0.0066 | 0.0103 | 0.0071 |
Bud Neck | 0.0034 | 0.0031 | 0.007 | 0.0024 | 0.0021 | 0.0117 | 0.002 | 0.0018 | 0.0027 | 0.0023 | 0.0015 | 0.007 | 0.024 | 0.0021 | 0.0058 | 0.0022 | 0.0028 | 0.0091 |
Bud Periphery | 0.0019 | 0.0051 | 0.0106 | 0.0151 | 0.0085 | 0.0032 | 0.0133 | 0.0058 | 0.0067 | 0.0157 | 0.0005 | 0.0023 | 0.0019 | 0.0061 | 0.0027 | 0.0043 | 0.0187 | 0.0057 |
Bud Site | 0.0099 | 0.0425 | 0.0108 | 0.0048 | 0.0044 | 0.0041 | 0.0074 | 0.0261 | 0.0144 | 0.0108 | 0.0018 | 0.0025 | 0.0237 | 0.049 | 0.0115 | 0.0024 | 0.0182 | 0.019 |
Cell Periphery | 0.0006 | 0.0011 | 0.0007 | 0.0009 | 0.0007 | 0.0006 | 0.001 | 0.0009 | 0.0009 | 0.0006 | 0.0001 | 0.0004 | 0.0004 | 0.0009 | 0.0005 | 0.0003 | 0.0006 | 0.0009 |
Cytoplasm | 0.2508 | 0.3905 | 0.3268 | 0.2479 | 0.3289 | 0.319 | 0.2589 | 0.3899 | 0.387 | 0.3151 | 0.4677 | 0.4024 | 0.3263 | 0.3888 | 0.5054 | 0.482 | 0.1223 | 0.3693 |
Cytoplasmic Foci | 0.1244 | 0.1159 | 0.1093 | 0.1802 | 0.0669 | 0.131 | 0.1001 | 0.1287 | 0.1272 | 0.0632 | 0.144 | 0.1208 | 0.1335 | 0.1176 | 0.0996 | 0.1385 | 0.1524 | 0.0789 |
Eisosomes | 0.0005 | 0.0002 | 0.0002 | 0.0002 | 0.0004 | 0.0001 | 0.0003 | 0.0002 | 0.0005 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0004 | 0.0001 | 0.0006 | 0.0003 |
Endoplasmic Reticulum | 0.0249 | 0.0128 | 0.0256 | 0.0083 | 0.021 | 0.0165 | 0.03 | 0.0124 | 0.0155 | 0.0059 | 0.0029 | 0.0315 | 0.0266 | 0.0148 | 0.0185 | 0.0053 | 0.0084 | 0.0242 |
Endosome | 0.2495 | 0.1107 | 0.1732 | 0.1673 | 0.1311 | 0.2108 | 0.2442 | 0.1342 | 0.1168 | 0.1311 | 0.1223 | 0.1776 | 0.1731 | 0.1357 | 0.1013 | 0.0842 | 0.1502 | 0.1513 |
Golgi | 0.0954 | 0.1003 | 0.1067 | 0.0826 | 0.1263 | 0.1088 | 0.0745 | 0.1351 | 0.108 | 0.1247 | 0.1585 | 0.102 | 0.0762 | 0.0846 | 0.0959 | 0.0897 | 0.1136 | 0.086 |
Lipid Particles | 0.0142 | 0.0034 | 0.0077 | 0.0028 | 0.0057 | 0.0073 | 0.0158 | 0.0012 | 0.0031 | 0.0232 | 0.0082 | 0.0023 | 0.0196 | 0.0027 | 0.0034 | 0.0074 | 0.0206 | 0.0017 |
Mitochondria | 0.0607 | 0.1047 | 0.1023 | 0.219 | 0.1303 | 0.0971 | 0.0782 | 0.0621 | 0.1103 | 0.1986 | 0.0234 | 0.0734 | 0.0302 | 0.0623 | 0.0408 | 0.0723 | 0.0906 | 0.1348 |
None | 0.0027 | 0.0078 | 0.0041 | 0.0033 | 0.0253 | 0.0032 | 0.0071 | 0.0017 | 0.002 | 0.0011 | 0.0016 | 0.0046 | 0.0364 | 0.0024 | 0.015 | 0.0016 | 0.0159 | 0.0043 |
Nuclear Periphery | 0.0243 | 0.0033 | 0.0122 | 0.0054 | 0.0125 | 0.007 | 0.018 | 0.0023 | 0.003 | 0.0097 | 0.0013 | 0.0098 | 0.0186 | 0.0045 | 0.0037 | 0.0019 | 0.0116 | 0.0287 |
Nucleolus | 0.0004 | 0.0016 | 0.0003 | 0.0005 | 0.0005 | 0.0002 | 0.0006 | 0.0001 | 0.0003 | 0.0005 | 0.0001 | 0.0007 | 0.0005 | 0.0003 | 0.0002 | 0.0001 | 0.0011 | 0.0003 |
Nucleus | 0.0188 | 0.0049 | 0.0082 | 0.006 | 0.003 | 0.0067 | 0.0212 | 0.0037 | 0.0076 | 0.0046 | 0.0044 | 0.0096 | 0.0057 | 0.0128 | 0.0037 | 0.0334 | 0.0037 | 0.0175 |
Peroxisomes | 0.0254 | 0.0191 | 0.026 | 0.0213 | 0.0121 | 0.0121 | 0.0214 | 0.0172 | 0.0117 | 0.0191 | 0.0388 | 0.0124 | 0.0153 | 0.0188 | 0.014 | 0.0343 | 0.1022 | 0.0077 |
Punctate Nuclear | 0.0024 | 0.0013 | 0.0023 | 0.0021 | 0.01 | 0.0033 | 0.0034 | 0.0007 | 0.0035 | 0.0004 | 0.0021 | 0.0016 | 0.0135 | 0.0014 | 0.0014 | 0.001 | 0.0248 | 0.0037 |
Vacuole | 0.0114 | 0.0183 | 0.0106 | 0.0062 | 0.0124 | 0.0085 | 0.0297 | 0.0058 | 0.0064 | 0.0085 | 0.0056 | 0.0097 | 0.0095 | 0.0079 | 0.0066 | 0.0074 | 0.005 | 0.0061 |
Vacuole Periphery | 0.0063 | 0.0027 | 0.0061 | 0.0037 | 0.0094 | 0.0043 | 0.0117 | 0.0026 | 0.0025 | 0.0078 | 0.0024 | 0.0049 | 0.0036 | 0.0025 | 0.002 | 0.0022 | 0.0041 | 0.0043 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 30.0928 | 50.8133 | 24.3978 | 36.7987 | 65.0278 | 26.7344 | 25.6886 | 19.3779 | 24.4892 | 35.553 |
Translational Efficiency | 3.4221 | 1.8929 | 3.5331 | 3.0988 | 3.7195 | 3.0959 | 2.6325 | 4.4127 | 2.8324 | 3.0744 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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