Standard name | |
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Human Ortholog | |
Description | Subunit of a two-component GTPase-activating protein, Bfa1p-Bub2p; contributes to GAP activity, inactivating Tem1 by stimulating GTP hydrolysis following damage or misalignment of the mitotic spindle; functions as a guanine-nucleotide exchange inhibitor (GDI) for Tem1p; involved in multiple cell cycle checkpoint pathways that control mitotic exit; required when telomeres are damaged, but not for all types of chromosomal DNA damage; phosphorylated by the Polo-like kinase Cdc5p |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 1 | 2 | 4 | 4 | 5 | 3 | – | 1 | 3 | 3 | 2 | 1 | 1 | 0 | 1 | 0 | 1 | 2 | 1 | 1 | 0 | 1 | 4 |
Bud | 16 | 8 | 4 | 8 | 7 | 16 | 25 | – | 15 | 27 | 31 | 23 | 1 | 2 | 2 | 3 | 2 | 3 | 10 | 9 | 4 | 5 | 6 | 7 |
Bud Neck | 10 | 6 | 11 | 6 | 6 | 12 | 15 | – | 7 | 19 | 12 | 11 | 5 | 2 | 4 | 2 | 4 | 2 | 3 | 8 | 0 | 3 | 3 | 2 |
Bud Site | 30 | 3 | 1 | 2 | 1 | 11 | 15 | – | 17 | 19 | 21 | 28 | 0 | 0 | 1 | 4 | 5 | 1 | – | – | – | – | – | – |
Cell Periphery | 2 | 1 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 63 | 33 | 22 | 30 | 22 | 25 | 36 | – | 25 | 30 | 32 | 20 | 40 | 62 | 72 | 27 | 35 | 20 | 25 | 16 | 9 | 36 | 85 | 116 |
Endoplasmic Reticulum | 0 | 1 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 36 | 51 | 4 | 1 | 2 | 7 | 6 | – | 4 | 2 | 2 | 3 | 8 | 14 | 31 | 16 | 23 | 20 | 16 | 18 | 6 | 4 | 5 | 3 |
Golgi | 0 | 1 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 1 | 2 | 1 | 1 | 0 | 0 | 0 |
Mitochondria | 19 | 10 | 1 | 6 | 2 | 13 | 16 | – | 27 | 21 | 43 | 35 | 0 | 0 | 0 | 6 | 7 | 4 | 4 | 5 | 7 | 0 | 1 | 4 |
Nucleus | 1 | 0 | 0 | 1 | 2 | 3 | 3 | – | 6 | 1 | 2 | 3 | 0 | 0 | 3 | 6 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 10 | 13 | 2 | 0 | 1 | 8 | 12 | – | 8 | 9 | 12 | 9 | 0 | 0 | 4 | 17 | 26 | 18 | 3 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 28 | 46 | 1 | 2 | 0 | 4 | 4 | – | 9 | 1 | 11 | 4 | 4 | 4 | 15 | 30 | 44 | 25 | 2 | 5 | 1 | 0 | 0 | 0 |
SpindlePole | 325 | 235 | 100 | 129 | 128 | 163 | 280 | – | 160 | 170 | 173 | 191 | 88 | 146 | 155 | 236 | 271 | 173 | 365 | 245 | 115 | 27 | 60 | 50 |
Vac/Vac Membrane | 5 | 0 | 1 | 0 | 0 | 3 | 1 | – | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 5 | 2 | 1 | 0 | 0 | 0 | 1 | 0 |
Unique Cell Count | 422 | 308 | 128 | 169 | 155 | 216 | 334 | 212 | 221 | 243 | 236 | 137 | 218 | 249 | 294 | 331 | 213 | 441 | 315 | 152 | 83 | 171 | 194 | |
Labelled Cell Count | 546 | 409 | 149 | 190 | 176 | 270 | 417 | 279 | 302 | 342 | 329 | 148 | 233 | 289 | 356 | 427 | 271 | 441 | 315 | 152 | 83 | 171 | 194 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.3 | 5.5 | 5.4 | 5.3 | 5.7 | 4.6 | 4.6 | – | 4.0 | 4.1 | 3.8 | 3.9 | 5.8 | 5.9 | 5.7 | 8.7 | 8.9 | 9.1 | 4.8 | 5.4 | 5.5 |
Std Deviation (1e-4) | 0.7 | 0.9 | 0.8 | 1.4 | 1.5 | 1.1 | 1.0 | – | 0.9 | 1.2 | 1.1 | 1.1 | 1.2 | 1.2 | 1.4 | 1.8 | 2.0 | 2.6 | 1.4 | 1.5 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.05 | 0.06 | -0.25 | -0.23 | – | -0.43 | -0.39 | -0.51 | -0.49 | 0.09 | 0.12 | 0.07 | 0.69 | 0.72 | 0.75 | -0.19 | -0.0 | 0.02 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0.7 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | -1.9 | -1.7 | 0 | 0 | – | 0 | 0 | 0 | 0 | -1.7 | 0 | -3.3 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.1 | -0.7 | -1.5 | -1.9 | – | -1.4 | -0.9 | -1.0 | -2.5 | 2.3 | 2.4 | 2.5 | -2.4 | -1.9 | -2.1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1.1 | 1.3 | 3.0 | 1.0 | 1.6 | 2.2 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.9 | 2.5 | 2.6 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 2.4 | 3.4 | 4.0 | 3.7 |
SpindlePole | -0.4 | 0.9 | 0 | 0 | – | 0 | 0 | 0 | 0 | -2.5 | -2.2 | -3.1 | 0.5 | 0.9 | 0.7 |
Vacuole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.4931 | 0.4294 | 0.268 | 0.2038 | 0.4585 | 0.1734 | 0.0922 | 0.4495 | 0.4511 | 0.4483 | 0.2324 | 0.5343 | 0.454 | 0.1198 | 0.0759 | 0.3301 | 0.4377 | 0.0401 |
Actin | 0.0012 | 0.0015 | 0.0008 | 0.0015 | 0.0005 | 0.0007 | 0.0087 | 0.0004 | 0.0057 | 0.0009 | 0.0031 | 0.0017 | 0.027 | 0.0019 | 0.0184 | 0.0001 | 0.0002 | 0.0003 |
Bud | 0.0001 | 0.0022 | 0.0004 | 0.0001 | 0.0005 | 0.0001 | 0.0026 | 0.0015 | 0.0017 | 0.0001 | 0.0025 | 0.0004 | 0.0077 | 0.0024 | 0.0015 | 0.0002 | 0.0001 | 0 |
Bud Neck | 0.0012 | 0.0045 | 0.0126 | 0.0653 | 0.0094 | 0.0051 | 0.0022 | 0.0042 | 0.0288 | 0.0972 | 0.0034 | 0.0065 | 0.0272 | 0.0011 | 0.0029 | 0.0026 | 0.0002 | 0.0007 |
Bud Periphery | 0.0001 | 0.0019 | 0.0004 | 0.0001 | 0.0011 | 0.0001 | 0.0024 | 0.0019 | 0.0009 | 0.0002 | 0.0038 | 0.0008 | 0.006 | 0.0048 | 0.0021 | 0.0001 | 0.0001 | 0 |
Bud Site | 0.0023 | 0.0244 | 0.0078 | 0.0018 | 0.0014 | 0.001 | 0.0037 | 0.0243 | 0.0045 | 0.0005 | 0.0099 | 0.0009 | 0.0156 | 0.008 | 0.0091 | 0.0003 | 0.0002 | 0.0002 |
Cell Periphery | 0 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0.0003 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.001 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Cytoplasm | 0.0066 | 0.016 | 0.0022 | 0.0023 | 0.0013 | 0.0042 | 0.0066 | 0.0174 | 0.0026 | 0.0014 | 0.0019 | 0.013 | 0.0048 | 0.0416 | 0.0044 | 0.0005 | 0.0004 | 0.0062 |
Cytoplasmic Foci | 0.3086 | 0.1307 | 0.1863 | 0.2895 | 0.2875 | 0.1688 | 0.2676 | 0.1863 | 0.1315 | 0.2551 | 0.2244 | 0.1469 | 0.3122 | 0.2914 | 0.2859 | 0.3476 | 0.5318 | 0.2813 |
Eisosomes | 0 | 0.0001 | 0 | 0.0001 | 0.0002 | 0 | 0.0016 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0002 | 0.0017 | 0.0003 | 0 | 0.0001 | 0.0002 | 0.0018 | 0.0007 | 0.0008 | 0.0015 | 0.0002 | 0.0006 | 0.0037 | 0.0019 | 0.0003 | 0 | 0.0001 | 0.0001 |
Endosome | 0.0069 | 0.0246 | 0.0058 | 0.0069 | 0.0045 | 0.0123 | 0.0163 | 0.0147 | 0.0064 | 0.0131 | 0.0055 | 0.0106 | 0.0253 | 0.017 | 0.0062 | 0.0007 | 0.0036 | 0.0089 |
Golgi | 0.0021 | 0.0041 | 0.0019 | 0.0031 | 0.0036 | 0.0019 | 0.0036 | 0.0031 | 0.0026 | 0.003 | 0.0041 | 0.0021 | 0.014 | 0.0041 | 0.0023 | 0.0009 | 0.0034 | 0.0026 |
Lipid Particles | 0.032 | 0.0458 | 0.0234 | 0.0211 | 0.0702 | 0.0248 | 0.0378 | 0.0266 | 0.0127 | 0.0367 | 0.0505 | 0.0152 | 0.0445 | 0.047 | 0.0128 | 0.0047 | 0.0844 | 0.0415 |
Mitochondria | 0.0001 | 0.0011 | 0.0002 | 0.0001 | 0.0007 | 0.0004 | 0.0101 | 0.0005 | 0.0029 | 0.0002 | 0.001 | 0.0014 | 0.0133 | 0.0079 | 0.0016 | 0.0002 | 0.0002 | 0 |
None | 0.053 | 0.0768 | 0.0204 | 0.0241 | 0.0417 | 0.0431 | 0.0343 | 0.063 | 0.0204 | 0.0202 | 0.0451 | 0.0602 | 0.004 | 0.0622 | 0.0115 | 0.0007 | 0.0006 | 0.0147 |
Nuclear Periphery | 0.0004 | 0.0045 | 0.001 | 0.0003 | 0.0006 | 0.0012 | 0.0043 | 0.0023 | 0.0026 | 0.0115 | 0.0012 | 0.0048 | 0.0107 | 0.0012 | 0.0004 | 0.0002 | 0.0003 | 0.0003 |
Nucleolus | 0.0085 | 0.0322 | 0.0178 | 0.0011 | 0.0097 | 0.0098 | 0.0021 | 0.0199 | 0.0146 | 0.0083 | 0.005 | 0.013 | 0.0039 | 0.0053 | 0.0016 | 0.0015 | 0.0008 | 0.001 |
Nucleus | 0.0069 | 0.0234 | 0.008 | 0.0005 | 0.0012 | 0.0067 | 0.0023 | 0.0139 | 0.01 | 0.0069 | 0.0017 | 0.0076 | 0.0088 | 0.0014 | 0.001 | 0.0006 | 0.0001 | 0.0005 |
Peroxisomes | 0.0387 | 0.0417 | 0.0228 | 0.0298 | 0.1214 | 0.0184 | 0.0284 | 0.0284 | 0.0214 | 0.012 | 0.0916 | 0.0152 | 0.0297 | 0.0263 | 0.0154 | 0.0063 | 0.0341 | 0.0124 |
Punctate Nuclear | 0.5307 | 0.559 | 0.6865 | 0.5522 | 0.4427 | 0.7004 | 0.5613 | 0.5856 | 0.7274 | 0.5286 | 0.5431 | 0.6973 | 0.4288 | 0.4717 | 0.6217 | 0.6327 | 0.3392 | 0.6292 |
Vacuole | 0.0003 | 0.003 | 0.0011 | 0.0001 | 0.0013 | 0.0005 | 0.0014 | 0.0048 | 0.0019 | 0.0022 | 0.0015 | 0.0014 | 0.0093 | 0.0024 | 0.0007 | 0 | 0.0001 | 0.0001 |
Vacuole Periphery | 0 | 0.0007 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0004 | 0.0003 | 0.0004 | 0.0005 | 0.0002 | 0.0004 | 0.0021 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 7.0362 | 13.1517 | 1.6972 | 3.1032 | 7.8787 | 3.8358 | 9.3951 | 6.6337 | 4.0738 | 10.5082 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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