Standard name
Human Ortholog
Description Protein involved in C/D snoRNP assembly; regulates abundance of Rsa1p; required for growth at high temperature; similar to human ZNHIT3
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.06 0 0.06 0.09 0.08 0.08 0.06 0 0.07 0.07 0.1 0.16 0.21 0.07 0.14 0.14 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0 0.6 0.59 0.42 0.77 0.73 0.65 0.81 0.75 0.73 0.74 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.93 0.93 0.83 0.85 0.74 0.61 0.63 0.65 0.64 0.67 0.63 0.63 0.85 0.78 0.74 0.89 0.73 0.79 0.89 0.87 0.83 0.81 0.66 0.64
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.05 0.13 0.11 0.05 0 0.09 0.08 0.21 0.18
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0
Bud 0 0 2 1 1 1 6 9 5 14 5 8 0 0 1 0 0 0 8 2 1 2 2 2
Bud Neck 0 0 0 0 1 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 1 1 8 17 11 20 14 17 13 0 1 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 19 10 17 6 16 22 37 33 24 21 27 25 19 42 53 8 16 15 4 0 4 2 0 2
Endoplasmic Reticulum 3 0 0 0 1 1 1 0 0 0 0 1 0 2 0 2 3 3 0 0 1 1 0 0
Endosome 2 0 1 0 0 0 0 0 0 0 0 0 1 3 3 0 0 1 3 4 1 2 1 3
Golgi 1 1 0 0 0 1 0 0 0 0 0 0 0 1 0 0 1 1 4 2 0 2 0 4
Mitochondria 67 3 176 118 110 190 358 272 318 351 274 279 2 3 2 1 2 1 14 4 6 0 1 2
Nucleus 789 243 241 170 194 151 306 271 252 315 235 237 159 210 191 104 86 86 769 239 243 157 70 101
Nuclear Periphery 8 0 3 0 0 0 8 2 5 2 1 4 0 0 0 1 0 0 1 0 0 0 0 1
Nucleolus 8 1 1 1 1 1 10 3 2 8 5 5 1 0 0 3 2 0 1 1 1 2 2 4
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 2 0 2 1 4 3 1 2 0 2 1 1 2 0 1 0 5 2 3 3 1 3
Vac/Vac Membrane 17 6 3 1 4 3 6 11 6 6 5 8 8 14 14 6 15 12 41 11 25 15 21 28
Unique Cell Count 848 262 291 199 263 247 489 418 392 468 375 379 187 270 258 117 118 109 863 275 293 193 106 157
Labelled Cell Count 914 265 447 298 331 379 753 616 633 734 569 582 191 277 266 125 126 119 863 275 293 193 106 157


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 6.1 3.6 3.6 3.9 3.3 3.2 3.6 3.0 3.4 3.3 3.3 5.9 5.9 5.6 9.6 10.2 9.3 6.3 6.5 6.7
Std Deviation (1e-4) 0.6 1.1 0.7 1.0 0.9 1.1 1.0 1.3 0.8 0.9 1.4 1.4 1.0 1.3 1.2 2.6 2.7 1.9 1.3 1.6 2.0
Intensity Change (Log2) – – – -0.02 0.1 -0.16 -0.21 -0.03 -0.3 -0.11 -0.16 -0.14 0.7 0.69 0.62 1.41 1.49 1.35 0.79 0.83 0.88


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.5 0.1 1.4 0.9 1.1 0.2 -0.8 0.7 0.4 1.7 3.7 5.2 0.4 2.6 2.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 -13.1 -15.1 -14.9 -11.0 -10.9 -10.7
Nucleus 0.8 -2.6 -5.6 -6.0 -5.3 -5.3 -4.7 -5.7 -5.8 0.6 -1.5 -2.5 1.5 -2.3 -0.9
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 1.5 0 0 0 0 0 2.9 3.0 0 5.2 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.019 0.221 -0.0531 -0.2889 -0.4589 -0.1102 0.5764 1.1862 0.699 0.7665 0.5159 0.6338 0.1798 0.2053 -0.0034 0.6148 0.9019 0.1427
Actin 0.0169 0.0003 0.0087 0.0251 0.0022 0.0035 0.0187 0.0007 0.0085 0.0108 0.0117 0.0016 0.0048 0 0.0083 0.0003 0.0021 0.0026
Bud 0.0022 0.0004 0.0006 0.0004 0.0012 0.0001 0.0002 0.0002 0.0013 0.0016 0.0033 0.0001 0.0005 0 0.0002 0.0001 0.001 0.0002
Bud Neck 0.0029 0.0002 0.0009 0.0004 0.0013 0.0008 0.0021 0.0023 0.0013 0.0008 0.0272 0.001 0.004 0.0001 0.0004 0.0009 0.0008 0.0098
Bud Periphery 0.0019 0.0001 0.0008 0.0006 0.0031 0.0001 0.0003 0.0002 0.0018 0.0044 0.0015 0.0002 0.001 0 0.0005 0.0001 0.0043 0.0004
Bud Site 0.0026 0.0016 0.0035 0.0033 0.0017 0.0002 0.0018 0.0021 0.0057 0.0031 0.0022 0.0001 0.0061 0 0.0007 0.0003 0.0017 0.0008
Cell Periphery 0.0003 0 0.0002 0.0001 0.0004 0 0.0001 0 0.0002 0.0002 0.0003 0 0.0004 0 0.0001 0 0.0002 0.0001
Cytoplasm 0.007 0.0098 0.0039 0.0051 0.014 0.0005 0.0039 0.002 0.0039 0.0025 0.0029 0.0034 0.0061 0.0017 0.0011 0.0007 0.0024 0.0012
Cytoplasmic Foci 0.0227 0.0013 0.008 0.0026 0.0161 0.0023 0.0219 0.0011 0.0049 0.0325 0.0045 0.0005 0.0113 0 0.0062 0.0004 0.0126 0.0033
Eisosomes 0.0002 0 0.0002 0.0002 0.0001 0 0.0002 0 0.0002 0.0002 0.0005 0 0.0001 0 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.0021 0.0005 0.0008 0.0009 0.0008 0.0008 0.0011 0.0001 0.0007 0.0005 0.0005 0.0014 0.0014 0.0002 0.0004 0.0001 0.0016 0.0004
Endosome 0.0158 0.0007 0.0196 0.0068 0.0021 0.0098 0.014 0.0005 0.0094 0.0365 0.0021 0.002 0.012 0.0001 0.0033 0.0008 0.0364 0.0049
Golgi 0.0044 0 0.004 0.0035 0.0006 0.0102 0.0048 0.0005 0.0036 0.0117 0.002 0.0007 0.0038 0 0.0018 0.0002 0.025 0.0021
Lipid Particles 0.0175 0.0002 0.0068 0.0043 0.0015 0.0111 0.0098 0.0003 0.0047 0.0397 0.0227 0.0006 0.0135 0 0.0016 0.0006 0.0287 0.0031
Mitochondria 0.0116 0.0003 0.012 0.0019 0.0053 0.0044 0.0016 0.0004 0.0024 0.0275 0.0017 0.0184 0.0029 0.0001 0.0095 0.0002 0.0344 0.0089
None 0.0108 0.0341 0.0106 0.0087 0.0582 0.0004 0.0073 0.0013 0.0008 0.002 0.0042 0.0006 0.0145 0.0005 0.0076 0.0003 0.0024 0.0005
Nuclear Periphery 0.0287 0.025 0.0094 0.0228 0.0494 0.0086 0.0061 0.0077 0.006 0.0037 0.0029 0.0039 0.012 0.006 0.0032 0.0074 0.0242 0.0115
Nucleolus 0.0182 0.0056 0.0062 0.0045 0.0168 0.0167 0.0059 0.0052 0.0034 0.0046 0.0196 0.0131 0.0051 0.0038 0.0037 0.0206 0.0195 0.0111
Nucleus 0.7626 0.8825 0.8712 0.8975 0.8123 0.9121 0.8799 0.971 0.9325 0.7179 0.8481 0.9445 0.8869 0.9841 0.9433 0.9286 0.7231 0.9219
Peroxisomes 0.0256 0 0.0087 0.0025 0.0008 0.0019 0.009 0.0004 0.0041 0.0366 0.0226 0.0004 0.0035 0 0.0039 0.0002 0.0111 0.0022
Punctate Nuclear 0.036 0.0358 0.0196 0.0078 0.0098 0.0153 0.01 0.0035 0.0025 0.0613 0.0187 0.007 0.0062 0.0031 0.0023 0.0381 0.037 0.0133
Vacuole 0.007 0.0008 0.0033 0.0005 0.0017 0.0005 0.001 0.0002 0.0018 0.0011 0.0006 0.0002 0.0027 0.0001 0.0011 0.0002 0.014 0.001
Vacuole Periphery 0.003 0.0004 0.0011 0.0005 0.001 0.0006 0.0004 0.0003 0.0004 0.0007 0.0003 0.0002 0.0013 0 0.0008 0.0001 0.0174 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 3.3436 1.9927 2.9573 7.7102 2.8901 2.2279 5.037 4.0796 6.3885 9.3623
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –
Standard name
Human Ortholog
Description Protein involved in C/D snoRNP assembly; regulates abundance of Rsa1p; required for growth at high temperature; similar to human ZNHIT3
Localization
Cell Percentages nucleus (97%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Hit1

Hit1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hit1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available