Standard name
Human Ortholog
Description Serine/threonine protein kinase; involved in regulation of ion transport across plasma membrane; carboxyl terminus is essential for glucose-dependent Pma1p activation via phosphorylation of Pma1p-Ser899; enhances spermine uptake; PTK2 has a paralog, PTK1, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.14 0.13 0.22 0.13 0.12 0.15 0.06 0.06 0.09 0.07 0.09 0.09 0.09 0.1 0.06 0.13 0.15 0.1 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.95 0.96 0.94 0.86 0.92 0.84 0.79 0.75 0.68 0.74 0.99 0.98 1.0 0.91 0.91 0.92 0.96 0.95 0.94 0.84 0.86 0.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.09 0.06
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.05 0.05 0.13 0.12 0.1 0.22 0.24 0.38 0.33 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.06 0 0.11 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 1 2 1 1 0 0 0 0 0 0 1 0 0 0 0 1 0
Bud 1 1 0 1 0 1 1 0 3 3 4 2 4 1 1 0 0 0 0 1 0 0 1 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 29 36 37 10 18 15 9 11 13 8 9 14 25 41 27 51 38 23 1 0 0 1 0 2
Cytoplasm 197 274 162 76 147 89 149 150 110 85 70 120 287 408 468 355 224 205 188 270 163 194 335 343
Endoplasmic Reticulum 0 4 0 0 0 1 2 0 3 2 1 3 3 11 1 12 10 7 2 2 1 25 33 22
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 3 0 0 1 0 1 0 1 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 2 0 0 1 0
Mitochondria 2 0 1 4 8 13 19 18 31 27 39 53 0 1 0 2 2 0 0 1 0 1 1 1
Nucleus 4 0 2 1 0 1 2 3 4 6 2 5 0 1 0 9 10 8 0 0 1 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 1 7 2 3 6 0 19 3 3 2 3 0 0 0 12 9 7 0 2 1 4 6 2
Unique Cell Count 201 279 171 79 156 103 162 179 140 114 103 163 291 418 469 389 247 223 197 283 175 231 390 381
Labelled Cell Count 233 316 209 94 176 126 183 202 169 136 128 200 319 466 497 444 294 251 197 283 175 231 390 381


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.3 12.5 9.4 8.1 8.9 7.8 7.4 8.6 6.8 6.3 6.3 6.7 10.1 11.4 12.1 13.7 13.9 12.7 9.2 9.4 10.4
Std Deviation (1e-4) 1.6 2.5 1.7 1.2 1.4 1.9 1.2 1.7 1.2 1.2 1.3 1.3 1.5 1.9 1.8 2.6 3.0 2.8 1.5 1.5 1.4
Intensity Change (Log2) -0.21 -0.07 -0.28 -0.35 -0.12 -0.47 -0.58 -0.58 -0.49 0.1 0.28 0.37 0.54 0.56 0.43 -0.03 0.01 0.14


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.7 -2.4 -1.4 -4.3 -4.2 -3.0 -3.3 -2.8 -3.3 -4.0 -3.8 -5.9 -2.6 -1.6 -3.1
Cytoplasm 0.5 -0.2 -2.4 -1.0 -3.3 -4.3 -4.9 -6.0 -5.3 2.4 1.8 4.6 -1.4 -1.5 -1.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.4 4.3 3.9 6.2 6.4 8.5 7.9 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 2.3 0 0 0 0 0 0 0 -0.6 -0.2 -0.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.422 9.6902 8.3505 7.8996 6.5466 8.5972 8.0651 10.5827 8.8661 6.9507 7.9736 9.294 7.0301 8.2682 6.9258 8.4368 5.6769 7.6133
Actin 0.0306 0.0007 0.0145 0.0026 0.0188 0.0086 0.0322 0.0019 0.0168 0.0709 0.0044 0.0028 0.0015 0.0021 0.0157 0.004 0.0267 0.0012
Bud 0.0007 0.0001 0.0033 0.0009 0.0008 0.0005 0.0013 0.0037 0.0008 0.0035 0.0006 0.0003 0.0001 0.0002 0.0005 0.0004 0.0011 0.0001
Bud Neck 0.0023 0.0004 0.0046 0.0021 0.0014 0.0033 0.0038 0.0004 0.0012 0.0016 0.002 0.0027 0.0003 0.0009 0.0015 0.0011 0.0016 0.002
Bud Periphery 0.0013 0.0001 0.0044 0.0021 0.0016 0.001 0.0018 0.0014 0.001 0.0052 0.0009 0.0009 0.0002 0.0002 0.0008 0.0004 0.0013 0.0002
Bud Site 0.0115 0.0021 0.008 0.0026 0.0024 0.0013 0.0085 0.0173 0.0016 0.0032 0.0007 0.0002 0.0006 0.004 0.0051 0.0006 0.0014 0.0001
Cell Periphery 0.0067 0.0043 0.0085 0.0044 0.0059 0.0031 0.0044 0.0029 0.0032 0.0016 0.002 0.0018 0.004 0.0023 0.0029 0.0015 0.0008 0.0013
Cytoplasm 0.7617 0.9802 0.9078 0.8776 0.7708 0.8383 0.7976 0.962 0.9008 0.619 0.8839 0.9421 0.9449 0.9496 0.9094 0.9277 0.8668 0.9759
Cytoplasmic Foci 0.0246 0.0008 0.0075 0.0046 0.0325 0.0065 0.0249 0.0023 0.0048 0.0183 0.0107 0.0046 0.0032 0.0035 0.0081 0.0075 0.0108 0.0023
Eisosomes 0.0006 0 0.0001 0.0001 0.0002 0.0003 0.0003 0 0.0001 0.0002 0.0001 0 0 0 0.0005 0 0.0002 0
Endoplasmic Reticulum 0.0366 0.008 0.0089 0.0065 0.0066 0.0231 0.0188 0.0027 0.0081 0.0064 0.0435 0.0128 0.0235 0.0249 0.0128 0.0036 0.0142 0.0102
Endosome 0.0239 0.0003 0.0063 0.0164 0.0337 0.0162 0.0297 0.0008 0.0203 0.0857 0.0273 0.0098 0.0057 0.0026 0.0108 0.0126 0.031 0.0028
Golgi 0.0117 0.0002 0.0046 0.0081 0.0426 0.0104 0.0217 0.0003 0.0092 0.0197 0.0123 0.0043 0.0018 0.0009 0.0046 0.0022 0.0138 0.0008
Lipid Particles 0.0191 0.0001 0.0054 0.0056 0.0196 0.0035 0.0103 0.0003 0.0041 0.0035 0.0011 0.0035 0.001 0.0009 0.0035 0.0022 0.0029 0.0003
Mitochondria 0.0087 0 0.0012 0.0539 0.0204 0.0424 0.0134 0.0001 0.0147 0.0727 0.0042 0.0049 0.0073 0.0002 0.0108 0.0003 0.0047 0.0002
None 0.0127 0.0011 0.0015 0.0007 0.0204 0.0217 0.0091 0.0008 0.0007 0.0036 0.0009 0.0005 0.0017 0.0034 0.0031 0.002 0.0045 0.0006
Nuclear Periphery 0.0092 0.0002 0.0004 0.0005 0.0012 0.0112 0.0017 0.0002 0.0024 0.0065 0.0007 0.0008 0.0005 0.0003 0.0017 0.0079 0.004 0.0003
Nucleolus 0.0011 0 0.0001 0.0002 0.0002 0.0004 0.0003 0.0001 0.0003 0.0003 0 0 0 0 0.0001 0.0003 0.0001 0
Nucleus 0.0035 0.0002 0.0008 0.0007 0.0007 0.0021 0.0013 0.0006 0.001 0.0026 0.0005 0.0005 0.0003 0.0004 0.0009 0.0022 0.0015 0.0003
Peroxisomes 0.0115 0 0.0063 0.0008 0.0161 0.0008 0.0095 0 0.0009 0.0107 0.0002 0.0052 0.0002 0 0.0015 0.0005 0.0068 0
Punctate Nuclear 0.0088 0 0.0002 0.0001 0.0011 0.0015 0.0009 0.0002 0.0004 0.0061 0.0001 0.0004 0.0001 0.0001 0.0013 0.0196 0.0009 0
Vacuole 0.0117 0.0012 0.0054 0.0085 0.0024 0.0022 0.0072 0.0017 0.0058 0.0393 0.0032 0.0013 0.003 0.0032 0.0039 0.003 0.0038 0.0011
Vacuole Periphery 0.0014 0 0.0002 0.0012 0.0007 0.0017 0.0013 0.0001 0.0017 0.0194 0.0006 0.0005 0.0001 0.0001 0.0004 0.0002 0.001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 17.0653 20.4742 21.1507 37.3003 26.4928 25.5837 33.4877 45.0669 41.0428 35.2405
Translational Efficiency 0.9114 0.9343 1.0371 0.7699 0.8533 0.9604 0.7544 0.7595 0.9352 0.7399

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Serine/threonine protein kinase; involved in regulation of ion transport across plasma membrane; carboxyl terminus is essential for glucose-dependent Pma1p activation via phosphorylation of Pma1p-Ser899; enhances spermine uptake; PTK2 has a paralog, PTK1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-4

Ptk2

Ptk2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ptk2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available