Standard name | |
---|---|
Human Ortholog | |
Description | Deoxyhypusine hydroxylase; HEAT-repeat containing metalloenzyme that catalyzes hypusine formation; binds to and is required for the modification of Hyp2p (eIF5A); complements S. pombe mmd1 mutants defective in mitochondrial positioning; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 1 | 7 | 1 | 9 | 8 | 4 | 6 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 4 | 0 | 0 | 2 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 1 | 4 | 1 | 4 | 4 | 10 | 5 | 22 | 11 | 4 | 9 | 1 | 6 | 7 | 1 | 8 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 451 | 282 | 184 | 186 | 229 | 256 | 316 | 228 | 238 | 195 | 137 | 137 | 392 | 656 | 738 | 255 | 333 | 273 | 431 | 258 | 174 | 206 | 363 | 420 |
Endoplasmic Reticulum | 1 | 1 | 2 | 0 | 2 | 6 | 23 | 17 | 17 | 12 | 3 | 8 | 3 | 1 | 2 | 13 | 11 | 13 | 0 | 0 | 1 | 0 | 4 | 5 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 3 | 0 | 1 | 3 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 1 | 1 | 0 | 8 | 2 | 15 | 11 | 15 | 9 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 1 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 5 | 4 | 1 | 2 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 1 | 0 | 1 | 2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 3 | 1 | 1 | 0 | 5 | 7 | 7 | 6 | 27 | 12 | 11 | 22 | 2 | 6 | 7 | 6 | 5 | 6 | 1 | 3 | 0 | 0 | 6 | 3 |
Unique Cell Count | 454 | 283 | 189 | 189 | 237 | 274 | 361 | 256 | 302 | 243 | 168 | 182 | 395 | 663 | 746 | 263 | 339 | 279 | 444 | 272 | 183 | 213 | 384 | 441 |
Labelled Cell Count | 456 | 285 | 191 | 189 | 242 | 279 | 377 | 261 | 335 | 254 | 176 | 195 | 398 | 669 | 754 | 278 | 360 | 300 | 444 | 272 | 183 | 213 | 384 | 441 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 41.7 | 50.5 | 33.2 | 31.2 | 24.6 | 19.7 | 17.9 | 18.5 | 16.6 | 16.9 | 15.0 | 16.4 | 36.8 | 36.9 | 36.0 | 71.2 | 78.3 | 74.6 | 45.5 | 43.2 | 44.8 |
Std Deviation (1e-4) | 7.5 | 11.2 | 6.9 | 7.1 | 5.3 | 4.2 | 3.9 | 4.0 | 3.9 | 4.0 | 3.7 | 3.5 | 8.9 | 8.0 | 6.8 | 24.5 | 26.4 | 27.2 | 10.7 | 10.3 | 10.2 |
Intensity Change (Log2) | – | – | – | -0.09 | -0.43 | -0.75 | -0.89 | -0.84 | -1.0 | -0.97 | -1.15 | -1.02 | 0.15 | 0.15 | 0.12 | 1.1 | 1.24 | 1.17 | 0.46 | 0.38 | 0.43 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 2.5 | 1.4 | 0 | 1.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.7 | -0.4 | -1.9 | -3.8 | -3.3 | -5.8 | -5.4 | -5.0 | -6.2 | 1.8 | 1.6 | 1.6 | -0.2 | 0.7 | 0.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 2.8 | 2.9 | 2.6 | 2.3 | 0 | 0 | 0 | 0 | 0 | 2.3 | 0 | 2.2 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 3.1 | 0 | 4.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 3.9 | 2.7 | 3.1 | 4.6 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 27.3809 | 33.2447 | 33.2041 | 32.4353 | 31.6006 | 30.585 | 37.0162 | 40.8041 | 40.4191 | 37.8774 | 37.1924 | 37.1793 | 40.7605 | 41.4513 | 40.1217 | 37.662 | 38.8551 | 38.4272 |
Actin | 0.0021 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0009 | 0.0001 | 0.0009 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0006 |
Bud | 0.0009 | 0.0001 | 0 | 0 | 0 | 0.0003 | 0.0001 | 0 | 0.0008 | 0.0003 | 0 | 0.0004 | 0.0001 | 0.0006 | 0.0007 | 0.0001 | 0.0004 | 0.0005 |
Bud Neck | 0.0007 | 0.0003 | 0.0002 | 0.0003 | 0.0003 | 0.0006 | 0.0002 | 0.0002 | 0.0003 | 0.0004 | 0.0004 | 0.0008 | 0.0002 | 0.0002 | 0.0002 | 0.0005 | 0.0004 | 0.0007 |
Bud Periphery | 0.0006 | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0002 |
Bud Site | 0.0004 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0003 | 0.0002 | 0 | 0.0001 | 0 |
Cell Periphery | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0 |
Cytoplasm | 0.9068 | 0.9825 | 0.9823 | 0.9828 | 0.9762 | 0.9625 | 0.957 | 0.9833 | 0.9764 | 0.9731 | 0.9674 | 0.9586 | 0.9567 | 0.9751 | 0.9702 | 0.9775 | 0.9796 | 0.9526 |
Cytoplasmic Foci | 0.0059 | 0.0003 | 0.0003 | 0.0003 | 0.0005 | 0.0004 | 0.0002 | 0.0002 | 0.0002 | 0.0005 | 0.0003 | 0.0004 | 0.0001 | 0.0006 | 0.0002 | 0.0002 | 0.0005 | 0.0006 |
Eisosomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0036 | 0.0006 | 0.0003 | 0.0006 | 0.0005 | 0.0007 | 0.0009 | 0.0006 | 0.0003 | 0.0006 | 0.0012 | 0.0008 | 0.0009 | 0.0006 | 0.001 | 0.0005 | 0.0004 | 0.0011 |
Endosome | 0.0093 | 0.0001 | 0.0001 | 0.0004 | 0.0002 | 0.0002 | 0.0003 | 0 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0003 |
Golgi | 0.0011 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Lipid Particles | 0.0009 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Mitochondria | 0.0047 | 0 | 0 | 0 | 0 | 0.0002 | 0.0007 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0006 | 0.0003 | 0 | 0 | 0.0001 |
None | 0.0042 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0003 | 0.0004 | 0.0002 | 0.0002 | 0.0011 | 0.0002 | 0.0005 | 0.0003 | 0.0007 | 0.0003 | 0.0002 | 0.0008 | 0.0004 |
Nuclear Periphery | 0.0072 | 0.0025 | 0.0018 | 0.0017 | 0.0029 | 0.0039 | 0.0044 | 0.0017 | 0.0017 | 0.002 | 0.0047 | 0.0037 | 0.0045 | 0.0026 | 0.0028 | 0.0023 | 0.0018 | 0.0051 |
Nucleolus | 0.0004 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0024 | 0.0002 | 0 | 0.0001 | 0.0001 |
Nucleus | 0.0417 | 0.0119 | 0.0143 | 0.0126 | 0.0182 | 0.0296 | 0.0333 | 0.013 | 0.0191 | 0.0195 | 0.0248 | 0.0324 | 0.0361 | 0.0141 | 0.0222 | 0.0176 | 0.0139 | 0.0361 |
Peroxisomes | 0.0003 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Punctate Nuclear | 0.0019 | 0.0002 | 0.0002 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0006 | 0.0002 | 0.0005 | 0.0001 | 0.0004 | 0.0002 | 0.0002 | 0.0009 | 0.0006 |
Vacuole | 0.0051 | 0.0005 | 0.0002 | 0.0007 | 0.0004 | 0.0007 | 0.0013 | 0.0004 | 0.0002 | 0.0004 | 0.0004 | 0.0005 | 0.0005 | 0.0007 | 0.0009 | 0.0006 | 0.0005 | 0.0008 |
Vacuole Periphery | 0.0022 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0006 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 239.1838 | 269.2867 | 205.8096 | 142.7671 | 184.312 | 168.0125 | 150.9243 | 116.3173 | 104.7273 | 146.5962 |
Translational Efficiency | 1.9654 | 2.094 | 2.044 | 2.306 | 2.5772 | 1.8578 | 1.961 | 2.4288 | 2.1527 | 2.1575 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|