Standard name
Human Ortholog
Description Member of the conserved GPN-loop GTPase family; has a role in transport of RNA polymerase II to the nucleus; exhibits GTP-dependent binding to PolII; has ATPase activity; involved in sister chromatid cohesion; phosphorylated by the Pcl1p-Pho85p kinase complex; human homolog XAB1 interacts with human RNA polymerase II; protein abundance increases in response to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0.06 0.07 0.06 0.07 0.07 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.96 0.92 0.95 0.91 0.85 0.66 0.66 0.68 0.61 0.58 0.6 0.98 0.99 0.98 0.96 0.96 0.9 0.95 0.95 0.9 0.96 0.96 0.93
Endoplasmic Reticulum 0 0 0.07 0 0 0 0.07 0.07 0.05 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.05 0.09 0.17 0.35 0.27 0.3 0.43 0.43 0.43 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.07 0.05 0 0 0.07 0 0 0 0 0 0.08 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 2 1 6 14 6 7 5 1 3 0 0 0 0 0 0 0 0 3 0 0 0
Bud 2 0 0 2 0 3 10 3 6 14 11 17 1 1 0 0 0 0 1 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 12 3 18 8 36 36 23 37 27 26 14 21 17 3 10 5 0 0 1 0 0 0
Cytoplasm 134 177 270 277 406 375 419 360 273 337 233 273 475 612 562 156 244 181 127 170 263 301 300 425
Endoplasmic Reticulum 0 4 19 4 10 13 45 39 22 24 13 11 11 13 8 3 5 3 1 2 13 1 2 10
Endosome 0 0 0 0 1 2 1 0 0 5 1 2 1 0 0 0 0 0 0 0 0 1 0 2
Golgi 1 0 1 1 1 1 0 0 0 0 0 0 0 0 0 0 0 1 1 0 2 0 0 0
Mitochondria 2 0 3 14 40 77 219 150 121 238 174 198 0 0 0 0 4 3 0 0 3 0 1 0
Nucleus 0 1 0 0 2 1 1 2 0 0 0 1 0 1 0 2 2 1 0 2 0 1 0 4
Nuclear Periphery 0 0 0 0 0 0 1 2 0 0 1 2 0 0 0 0 0 0 0 0 0 0 0 2
Nucleolus 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 1 2 2 9 10 23 36 22 24 18 30 1 0 3 4 7 17 0 2 0 5 4 4
Unique Cell Count 135 184 292 291 445 440 633 547 402 548 402 456 483 621 571 162 255 202 134 179 294 314 315 460
Labelled Cell Count 139 184 307 305 488 496 770 635 474 685 479 563 503 648 590 168 272 212 134 179 294 314 315 460


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.3 16.1 13.3 12.1 10.9 9.4 8.6 8.5 8.0 7.6 7.2 7.1 15.3 14.9 14.3 22.0 24.5 23.3 15.8 15.7 16.3
Std Deviation (1e-4) 2.1 2.9 2.1 1.7 1.5 1.6 1.6 1.7 1.6 1.7 1.4 1.5 2.8 2.7 2.6 7.8 8.1 7.8 2.6 2.9 3.2
Intensity Change (Log2) -0.14 -0.29 -0.51 -0.63 -0.64 -0.73 -0.81 -0.9 -0.91 0.2 0.16 0.1 0.73 0.88 0.81 0.24 0.24 0.29


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 3.3 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -2.3 0 -1.9 1.0 1.5 1.0 1.6 1.5 1.0 -0.9 -0.5 -0.9 -1.3 -0.1 -1.0
Cytoplasm 1.4 -0.6 -3.0 -8.5 -8.5 -7.7 -9.5 -10.0 -9.8 4.1 4.7 4.5 1.6 1.6 -1.1
Endoplasmic Reticulum -3.2 -2.9 -2.3 0.3 0.3 -0.6 -1.3 -2.0 -2.8 -3.0 -3.4 -4.1 -2.2 -2.6 -2.7
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.7 4.5 7.0 11.1 9.4 9.9 12.9 12.6 12.8 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 2.6 3.9 3.4 2.9 2.9 3.9 0 0 0 0 0 4.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 10.7598 14.1615 12.6967 12.322 10.8947 12.7188 9.1941 13.3073 12.2707 10.6878 11.3606 12.2369 6.8945 11.7274 9.8708 8.8211 9.9018 10.8437
Actin 0.021 0.0002 0.0029 0.0007 0.0378 0.0024 0.0188 0.0004 0.0042 0.0189 0.0011 0.0007 0.0296 0.0011 0.0071 0.0225 0.0004 0.0002
Bud 0.0052 0.0002 0.006 0.0004 0.0005 0.0005 0.0008 0.0002 0.0002 0.0014 0.0002 0.0002 0.0019 0.0002 0.0004 0.0012 0.0004 0.0002
Bud Neck 0.0012 0.0002 0.0007 0.0007 0.0007 0.0013 0.0037 0.0002 0.0004 0.0013 0.0004 0.001 0.0028 0.0001 0.0005 0.0006 0.0005 0.0009
Bud Periphery 0.0022 0.0001 0.0025 0.0001 0.0003 0.0003 0.0008 0.0001 0.0001 0.0013 0.0001 0.0001 0.0015 0.0001 0.0001 0.0014 0.0001 0
Bud Site 0.0054 0.0004 0.0023 0.0004 0.0005 0.0001 0.0019 0.0005 0.0003 0.0026 0.0003 0 0.0075 0.0003 0.0007 0.0034 0.0002 0
Cell Periphery 0.0006 0.0002 0.0004 0.0002 0.0002 0.0002 0.0004 0.0002 0.0002 0.0003 0.0001 0.0002 0.0006 0.0001 0.0001 0.0004 0 0.0001
Cytoplasm 0.8236 0.9542 0.8907 0.8128 0.8098 0.892 0.7937 0.9568 0.9091 0.7762 0.8757 0.9132 0.7419 0.9571 0.8792 0.7851 0.8635 0.9484
Cytoplasmic Foci 0.0234 0.0079 0.0131 0.0215 0.0157 0.0123 0.017 0.0058 0.0105 0.0236 0.0216 0.0103 0.0328 0.0054 0.0135 0.0315 0.0133 0.0088
Eisosomes 0.0005 0 0 0 0.0001 0 0.0001 0 0 0.0001 0 0 0.0002 0 0 0.0002 0 0
Endoplasmic Reticulum 0.0293 0.0106 0.0214 0.0074 0.0159 0.0274 0.0412 0.0138 0.0087 0.0144 0.0071 0.0357 0.0192 0.0104 0.0115 0.0122 0.0101 0.0116
Endosome 0.0432 0.0192 0.0365 0.116 0.0752 0.0337 0.0519 0.0125 0.0322 0.0797 0.0634 0.0256 0.0648 0.0126 0.0431 0.0531 0.0786 0.0219
Golgi 0.0086 0.0015 0.004 0.0061 0.0073 0.0057 0.017 0.0021 0.0021 0.0121 0.0066 0.0041 0.0254 0.0022 0.013 0.0294 0.0173 0.0019
Lipid Particles 0.0052 0 0.0009 0.0001 0.0007 0.0006 0.0056 0.0001 0.0029 0.0016 0.0058 0.0001 0.0149 0.0004 0.0017 0.0025 0.0025 0
Mitochondria 0.0056 0.0004 0.0019 0.0011 0.0048 0.0075 0.0135 0.0005 0.0078 0.0184 0.0045 0.0015 0.0136 0.0025 0.0132 0.0276 0.0015 0.0012
None 0.0046 0.0005 0.0013 0.0026 0.0006 0.0031 0.0036 0.0005 0.001 0.0017 0.0008 0.0005 0.0029 0.0022 0.001 0.0006 0.0003 0.0003
Nuclear Periphery 0.0027 0.0004 0.0036 0.0043 0.0017 0.0067 0.0072 0.0008 0.0021 0.0075 0.0017 0.0017 0.0115 0.001 0.0033 0.0015 0.0025 0.0005
Nucleolus 0.0002 0 0.0001 0.0001 0 0 0.0002 0 0 0.0001 0.0001 0 0.0003 0 0 0.0001 0 0
Nucleus 0.0015 0.0005 0.0015 0.0067 0.0013 0.001 0.0041 0.0009 0.001 0.0034 0.0012 0.001 0.0072 0.001 0.0012 0.001 0.0009 0.0007
Peroxisomes 0.0045 0 0.0002 0.0001 0.0028 0.0002 0.0026 0 0.0043 0.0028 0.0036 0 0.0073 0.0001 0.0009 0.0061 0.0003 0
Punctate Nuclear 0.0028 0 0.0006 0.0009 0.0001 0.0003 0.004 0.0001 0.0006 0.0005 0.0008 0.0001 0.0022 0.0004 0.0011 0.0003 0.0003 0
Vacuole 0.0069 0.0028 0.0077 0.0138 0.0207 0.0029 0.0093 0.0039 0.01 0.0242 0.0033 0.0028 0.0088 0.0024 0.0067 0.0148 0.0045 0.0022
Vacuole Periphery 0.0017 0.0007 0.0018 0.004 0.0032 0.0017 0.0025 0.0006 0.0025 0.0077 0.0017 0.001 0.003 0.0005 0.0018 0.0046 0.0026 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 92.4268 63.8845 77.1122 64.8462 69.1833 68.5675 55.3351 65.9757 58.7128 58.8145
Translational Efficiency 1.1734 1.5563 1.1825 1.4838 1.2994 1.2842 1.3892 1.102 1.2282 1.2417

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Member of the conserved GPN-loop GTPase family; has a role in transport of RNA polymerase II to the nucleus; exhibits GTP-dependent binding to PolII; has ATPase activity; involved in sister chromatid cohesion; phosphorylated by the Pcl1p-Pho85p kinase complex; human homolog XAB1 interacts with human RNA polymerase II; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Npa3

Npa3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Npa3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available